Clinical-scale selection and viral transduction of human naïve and central memory CD8+ T cells for adoptive cell therapy of cancer ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2013-10

AUTHORS

Anna Casati, Azam Varghaei-Nahvi, Steven Alexander Feldman, Mario Assenmacher, Steven Aaron Rosenberg, Mark Edward Dudley, Alexander Scheffold

ABSTRACT

The adoptive transfer of lymphocytes genetically engineered to express tumor-specific antigen receptors is a potent strategy to treat cancer patients. T lymphocyte subsets, such as naïve or central memory T cells, selected in vitro prior to genetic engineering have been extensively investigated in preclinical mouse models, where they demonstrated improved therapeutic efficacy. However, so far, this is challenging to realize in the clinical setting, since good manufacturing practices (GMP) procedures for complex cell sorting and genetic manipulation are limited. To be able to directly compare the immunological attributes and therapeutic efficacy of naïve (T(N)) and central memory (T(CM)) CD8(+) T cells, we investigated clinical-scale procedures for their parallel selection and in vitro manipulation. We also evaluated currently available GMP-grade reagents for stimulation of T cell subsets, including a new type of anti-CD3/anti-CD28 nanomatrix. An optimized protocol was established for the isolation of both CD8(+) T(N) cells (CD4(-)CD62L(+)CD45RA(+)) and CD8(+) T(CM) (CD4(-)CD62L(+)CD45RA(-)) from a single patient. The highly enriched T cell subsets can be efficiently transduced and expanded to large cell numbers, sufficient for clinical applications and equivalent to or better than current cell and gene therapy approaches with unselected lymphocyte populations. The GMP protocols for selection of T(N) and T(CM) we reported here will be the basis for clinical trials analyzing safety, in vivo persistence and clinical efficacy in cancer patients and will help to generate a more reliable and efficacious cellular product. More... »

PAGES

1563-1573

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00262-013-1459-x

DOI

http://dx.doi.org/10.1007/s00262-013-1459-x

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1013158633

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/23903715


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1107", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Immunology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "CD8-Positive T-Lymphocytes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Differentiation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Immunologic Memory", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Immunophenotyping", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Immunotherapy, Adoptive", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Melanoma", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "T-Lymphocyte Subsets", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transduction, Genetic", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Miltenyi Biotec (Germany)", 
          "id": "https://www.grid.ac/institutes/grid.59409.31", 
          "name": [
            "Research and Development, Miltenyi Biotec GmbH, Bergisch Gladbach, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Casati", 
        "givenName": "Anna", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Institute of Child Health and Human Development", 
          "id": "https://www.grid.ac/institutes/grid.420089.7", 
          "name": [
            "Surgery Branch, National Cancer Institute, National Institute of Health, 20892, Bethesda, MD, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Varghaei-Nahvi", 
        "givenName": "Azam", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Institute of Child Health and Human Development", 
          "id": "https://www.grid.ac/institutes/grid.420089.7", 
          "name": [
            "Surgery Branch, National Cancer Institute, National Institute of Health, 20892, Bethesda, MD, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Feldman", 
        "givenName": "Steven Alexander", 
        "id": "sg:person.01137060061.40", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01137060061.40"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Miltenyi Biotec (Germany)", 
          "id": "https://www.grid.ac/institutes/grid.59409.31", 
          "name": [
            "Research and Development, Miltenyi Biotec GmbH, Bergisch Gladbach, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Assenmacher", 
        "givenName": "Mario", 
        "id": "sg:person.01160641160.49", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01160641160.49"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Institute of Child Health and Human Development", 
          "id": "https://www.grid.ac/institutes/grid.420089.7", 
          "name": [
            "Surgery Branch, National Cancer Institute, National Institute of Health, 20892, Bethesda, MD, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Rosenberg", 
        "givenName": "Steven Aaron", 
        "id": "sg:person.0717624506.16", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0717624506.16"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "National Institute of Child Health and Human Development", 
          "id": "https://www.grid.ac/institutes/grid.420089.7", 
          "name": [
            "Surgery Branch, National Cancer Institute, National Institute of Health, 20892, Bethesda, MD, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Dudley", 
        "givenName": "Mark Edward", 
        "id": "sg:person.0607775241.72", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0607775241.72"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Leibniz Association", 
          "id": "https://www.grid.ac/institutes/grid.413453.4", 
          "name": [
            "Department of Rheumatology and Clinical Immunology, Charit\u00e9, Universit\u00e4tsmedizin Berlin, Charit\u00e9platz 1, 10117, Berlin, Germany", 
            "German Rheumatism Research Centre (DRFZ) Berlin, Leibniz Association, Berlin, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Scheffold", 
        "givenName": "Alexander", 
        "id": "sg:person.01107745373.14", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01107745373.14"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/j.immuni.2009.09.014", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001113648"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/01.cji.0000158855.92792.7a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002151888"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/01.cji.0000158855.92792.7a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002151888"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1056/nejm200010053431407", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002831560"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1056/nejmoa1103849", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009197831"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/ijc.2910510623", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011270012"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0907448106", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011880450"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1113748109", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012607217"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nri3191", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013056074", 
          "https://doi.org/10.1038/nri3191"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/eji.200939555", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013787753"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00002371-200307000-00005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014162674"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00002371-200307000-00005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014162674"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/00002371-200307000-00005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014162674"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/44385", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016546162", 
          "https://doi.org/10.1038/44385"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/44385", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016546162", 
          "https://doi.org/10.1038/44385"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1200/jco.2010.32.2537", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017008867"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.92.17.7739", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017993705"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1182/blood-2008-05-156059", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020008181"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/scitranslmed.3003634", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020921605"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.1129003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021480946"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/cji.0b013e31819c8bda", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028333928"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/cji.0b013e31819c8bda", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028333928"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1097/cji.0b013e31819c8bda", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028333928"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.coi.2010.01.020", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030001269"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0503726102", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030180802"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1182/blood-2009-03-211714", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031594949"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4049/jimmunol.175.10.7046", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031627761"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1182/blood-2010-04-281931", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033707875"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1182/blood-2010-05-286286", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034635209"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1158/1078-0432.ccr-11-0116", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034962577"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1535-6108(03)00113-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035078557"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nri1842", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036296603", 
          "https://doi.org/10.1038/nri1842"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nri1842", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036296603", 
          "https://doi.org/10.1038/nri1842"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1182/blood-2010-07-294520", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037865345"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1182/blood-2002-11-3577", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037916496"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0307298101", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038276939"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1182/blood-2010-10-310599", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038542835"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/mt.2010.24", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039784712"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1182/blood-2007-09-113050", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040845426"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt0202-143", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046168734", 
          "https://doi.org/10.1038/nbt0202-143"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt0202-143", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046168734", 
          "https://doi.org/10.1038/nbt0202-143"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci43490", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046210483"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci43490", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046210483"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1056/nejmoa0800251", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050025318"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nm.1882", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051448639", 
          "https://doi.org/10.1038/nm.1882"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci24480", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053677825"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1038/mt.2010.272", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053678344"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/hum.2010.064", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059273502"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.272.5270.1939", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062553450"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1172/jci32103", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1063416958"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2174/156652311796150390", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1069184639"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4049/jimmunol.176.12.7726", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077250169"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4049/jimmunol.177.9.6548", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077312039"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4049/jimmunol.0902047", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1078152676"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2013-10", 
    "datePublishedReg": "2013-10-01", 
    "description": "The adoptive transfer of lymphocytes genetically engineered to express tumor-specific antigen receptors is a potent strategy to treat cancer patients. T lymphocyte subsets, such as na\u00efve or central memory T cells, selected in vitro prior to genetic engineering have been extensively investigated in preclinical mouse models, where they demonstrated improved therapeutic efficacy. However, so far, this is challenging to realize in the clinical setting, since good manufacturing practices (GMP) procedures for complex cell sorting and genetic manipulation are limited. To be able to directly compare the immunological attributes and therapeutic efficacy of na\u00efve (T(N)) and central memory (T(CM)) CD8(+) T cells, we investigated clinical-scale procedures for their parallel selection and in vitro manipulation. We also evaluated currently available GMP-grade reagents for stimulation of T cell subsets, including a new type of anti-CD3/anti-CD28 nanomatrix. An optimized protocol was established for the isolation of both CD8(+) T(N) cells (CD4(-)CD62L(+)CD45RA(+)) and CD8(+) T(CM) (CD4(-)CD62L(+)CD45RA(-)) from a single patient. The highly enriched T cell subsets can be efficiently transduced and expanded to large cell numbers, sufficient for clinical applications and equivalent to or better than current cell and gene therapy approaches with unselected lymphocyte populations. The GMP protocols for selection of T(N) and T(CM) we reported here will be the basis for clinical trials analyzing safety, in vivo persistence and clinical efficacy in cancer patients and will help to generate a more reliable and efficacious cellular product.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s00262-013-1459-x", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2723975", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2726503", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1096240", 
        "issn": [
          "0340-7004", 
          "1432-0851"
        ], 
        "name": "Cancer Immunology, Immunotherapy", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "10", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "62"
      }
    ], 
    "name": "Clinical-scale selection and viral transduction of human na\u00efve and central memory CD8+ T cells for adoptive cell therapy of cancer patients", 
    "pagination": "1563-1573", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "3eaf1b6ee1c9c5b44a3435504e9eaf40248c1fd342be596a40ec7bef32e808bf"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "23903715"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "8605732"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00262-013-1459-x"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1013158633"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00262-013-1459-x", 
      "https://app.dimensions.ai/details/publication/pub.1013158633"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T20:01", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8681_00000531.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007%2Fs00262-013-1459-x"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00262-013-1459-x'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00262-013-1459-x'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00262-013-1459-x'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00262-013-1459-x'


 

This table displays all metadata directly associated to this object as RDF triples.

296 TRIPLES      21 PREDICATES      83 URIs      30 LITERALS      18 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00262-013-1459-x schema:about N28d65854f712496eb90be42f82021d1d
2 N2ad414bbf19c4f1c9dd65209735f2885
3 N2fe8372f50ba4323b51d611667d0920e
4 N38b6a6d2ef9f4213990d0f5f1aadc7f5
5 N6091be3276fe4c1d9a96db5593bb3774
6 N73245cf7bbd942ad9bf6f6e62161ed2c
7 N7a24c6f1bade4e88a5da89426af42407
8 Na5746bf840d247eab2fba91366a08464
9 Nb6071c93391d45c69236dd9775ec9e05
10 anzsrc-for:11
11 anzsrc-for:1107
12 schema:author N4163dc3c8b6c414f9c0cb3ae73cca7c5
13 schema:citation sg:pub.10.1038/44385
14 sg:pub.10.1038/nbt0202-143
15 sg:pub.10.1038/nm.1882
16 sg:pub.10.1038/nri1842
17 sg:pub.10.1038/nri3191
18 https://doi.org/10.1002/eji.200939555
19 https://doi.org/10.1002/ijc.2910510623
20 https://doi.org/10.1016/j.coi.2010.01.020
21 https://doi.org/10.1016/j.immuni.2009.09.014
22 https://doi.org/10.1016/s1535-6108(03)00113-2
23 https://doi.org/10.1038/mt.2010.24
24 https://doi.org/10.1038/mt.2010.272
25 https://doi.org/10.1056/nejm200010053431407
26 https://doi.org/10.1056/nejmoa0800251
27 https://doi.org/10.1056/nejmoa1103849
28 https://doi.org/10.1073/pnas.0307298101
29 https://doi.org/10.1073/pnas.0503726102
30 https://doi.org/10.1073/pnas.0907448106
31 https://doi.org/10.1073/pnas.1113748109
32 https://doi.org/10.1073/pnas.92.17.7739
33 https://doi.org/10.1089/hum.2010.064
34 https://doi.org/10.1097/00002371-200307000-00005
35 https://doi.org/10.1097/01.cji.0000158855.92792.7a
36 https://doi.org/10.1097/cji.0b013e31819c8bda
37 https://doi.org/10.1126/science.1129003
38 https://doi.org/10.1126/science.272.5270.1939
39 https://doi.org/10.1126/scitranslmed.3003634
40 https://doi.org/10.1158/1078-0432.ccr-11-0116
41 https://doi.org/10.1172/jci24480
42 https://doi.org/10.1172/jci32103
43 https://doi.org/10.1172/jci43490
44 https://doi.org/10.1182/blood-2002-11-3577
45 https://doi.org/10.1182/blood-2007-09-113050
46 https://doi.org/10.1182/blood-2008-05-156059
47 https://doi.org/10.1182/blood-2009-03-211714
48 https://doi.org/10.1182/blood-2010-04-281931
49 https://doi.org/10.1182/blood-2010-05-286286
50 https://doi.org/10.1182/blood-2010-07-294520
51 https://doi.org/10.1182/blood-2010-10-310599
52 https://doi.org/10.1200/jco.2010.32.2537
53 https://doi.org/10.2174/156652311796150390
54 https://doi.org/10.4049/jimmunol.0902047
55 https://doi.org/10.4049/jimmunol.175.10.7046
56 https://doi.org/10.4049/jimmunol.176.12.7726
57 https://doi.org/10.4049/jimmunol.177.9.6548
58 schema:datePublished 2013-10
59 schema:datePublishedReg 2013-10-01
60 schema:description The adoptive transfer of lymphocytes genetically engineered to express tumor-specific antigen receptors is a potent strategy to treat cancer patients. T lymphocyte subsets, such as naïve or central memory T cells, selected in vitro prior to genetic engineering have been extensively investigated in preclinical mouse models, where they demonstrated improved therapeutic efficacy. However, so far, this is challenging to realize in the clinical setting, since good manufacturing practices (GMP) procedures for complex cell sorting and genetic manipulation are limited. To be able to directly compare the immunological attributes and therapeutic efficacy of naïve (T(N)) and central memory (T(CM)) CD8(+) T cells, we investigated clinical-scale procedures for their parallel selection and in vitro manipulation. We also evaluated currently available GMP-grade reagents for stimulation of T cell subsets, including a new type of anti-CD3/anti-CD28 nanomatrix. An optimized protocol was established for the isolation of both CD8(+) T(N) cells (CD4(-)CD62L(+)CD45RA(+)) and CD8(+) T(CM) (CD4(-)CD62L(+)CD45RA(-)) from a single patient. The highly enriched T cell subsets can be efficiently transduced and expanded to large cell numbers, sufficient for clinical applications and equivalent to or better than current cell and gene therapy approaches with unselected lymphocyte populations. The GMP protocols for selection of T(N) and T(CM) we reported here will be the basis for clinical trials analyzing safety, in vivo persistence and clinical efficacy in cancer patients and will help to generate a more reliable and efficacious cellular product.
61 schema:genre research_article
62 schema:inLanguage en
63 schema:isAccessibleForFree false
64 schema:isPartOf N13752f8b026f4bab84699b0ae5ed26cd
65 N257680c0320240c6b98fded8dbd11a9c
66 sg:journal.1096240
67 schema:name Clinical-scale selection and viral transduction of human naïve and central memory CD8+ T cells for adoptive cell therapy of cancer patients
68 schema:pagination 1563-1573
69 schema:productId N02fdb3aeaaab4792984a71be7c35a613
70 N0c058b8aa4334cf999d123111651f9c4
71 Na5a8d6fa979743298b74c5bb056cd1c6
72 Naf61c0bdb7b54ee1a02acd7888cd3bb8
73 Nc41c54449094485aab5904f78d4ee42f
74 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013158633
75 https://doi.org/10.1007/s00262-013-1459-x
76 schema:sdDatePublished 2019-04-10T20:01
77 schema:sdLicense https://scigraph.springernature.com/explorer/license/
78 schema:sdPublisher Na5a581ada17d43f48eace6de66141197
79 schema:url http://link.springer.com/10.1007%2Fs00262-013-1459-x
80 sgo:license sg:explorer/license/
81 sgo:sdDataset articles
82 rdf:type schema:ScholarlyArticle
83 N02fdb3aeaaab4792984a71be7c35a613 schema:name readcube_id
84 schema:value 3eaf1b6ee1c9c5b44a3435504e9eaf40248c1fd342be596a40ec7bef32e808bf
85 rdf:type schema:PropertyValue
86 N0c058b8aa4334cf999d123111651f9c4 schema:name doi
87 schema:value 10.1007/s00262-013-1459-x
88 rdf:type schema:PropertyValue
89 N13752f8b026f4bab84699b0ae5ed26cd schema:issueNumber 10
90 rdf:type schema:PublicationIssue
91 N257680c0320240c6b98fded8dbd11a9c schema:volumeNumber 62
92 rdf:type schema:PublicationVolume
93 N28d65854f712496eb90be42f82021d1d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
94 schema:name Immunotherapy, Adoptive
95 rdf:type schema:DefinedTerm
96 N2ad414bbf19c4f1c9dd65209735f2885 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
97 schema:name Cell Differentiation
98 rdf:type schema:DefinedTerm
99 N2fe8372f50ba4323b51d611667d0920e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
100 schema:name T-Lymphocyte Subsets
101 rdf:type schema:DefinedTerm
102 N38b6a6d2ef9f4213990d0f5f1aadc7f5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
103 schema:name Humans
104 rdf:type schema:DefinedTerm
105 N4163dc3c8b6c414f9c0cb3ae73cca7c5 rdf:first N4792b5ef947445a890cf7b2b98e04e40
106 rdf:rest Ndc2070ceca164b81905868a1d6174227
107 N4792b5ef947445a890cf7b2b98e04e40 schema:affiliation https://www.grid.ac/institutes/grid.59409.31
108 schema:familyName Casati
109 schema:givenName Anna
110 rdf:type schema:Person
111 N6091be3276fe4c1d9a96db5593bb3774 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name CD8-Positive T-Lymphocytes
113 rdf:type schema:DefinedTerm
114 N73245cf7bbd942ad9bf6f6e62161ed2c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
115 schema:name Melanoma
116 rdf:type schema:DefinedTerm
117 N7a24c6f1bade4e88a5da89426af42407 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Immunophenotyping
119 rdf:type schema:DefinedTerm
120 N8274f1b9b6f046b0bfd3a4c9b53589b1 rdf:first sg:person.01137060061.40
121 rdf:rest Ne9151f62fa3b469aa3b18ddde13b102a
122 N82fa9d53cbb94c9a83c6ba43fdaa2933 rdf:first sg:person.0607775241.72
123 rdf:rest N9ce3c792c04c492b8bd4232de9c1aac3
124 N852328ab656e460c934dd25651ac23e6 rdf:first sg:person.0717624506.16
125 rdf:rest N82fa9d53cbb94c9a83c6ba43fdaa2933
126 N9ce3c792c04c492b8bd4232de9c1aac3 rdf:first sg:person.01107745373.14
127 rdf:rest rdf:nil
128 Na5746bf840d247eab2fba91366a08464 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
129 schema:name Transduction, Genetic
130 rdf:type schema:DefinedTerm
131 Na5a581ada17d43f48eace6de66141197 schema:name Springer Nature - SN SciGraph project
132 rdf:type schema:Organization
133 Na5a8d6fa979743298b74c5bb056cd1c6 schema:name nlm_unique_id
134 schema:value 8605732
135 rdf:type schema:PropertyValue
136 Naf61c0bdb7b54ee1a02acd7888cd3bb8 schema:name dimensions_id
137 schema:value pub.1013158633
138 rdf:type schema:PropertyValue
139 Nb6071c93391d45c69236dd9775ec9e05 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
140 schema:name Immunologic Memory
141 rdf:type schema:DefinedTerm
142 Nc41c54449094485aab5904f78d4ee42f schema:name pubmed_id
143 schema:value 23903715
144 rdf:type schema:PropertyValue
145 Ndc2070ceca164b81905868a1d6174227 rdf:first Nfd54eb90fa99416b94400f86385bb3ea
146 rdf:rest N8274f1b9b6f046b0bfd3a4c9b53589b1
147 Ne9151f62fa3b469aa3b18ddde13b102a rdf:first sg:person.01160641160.49
148 rdf:rest N852328ab656e460c934dd25651ac23e6
149 Nfd54eb90fa99416b94400f86385bb3ea schema:affiliation https://www.grid.ac/institutes/grid.420089.7
150 schema:familyName Varghaei-Nahvi
151 schema:givenName Azam
152 rdf:type schema:Person
153 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
154 schema:name Medical and Health Sciences
155 rdf:type schema:DefinedTerm
156 anzsrc-for:1107 schema:inDefinedTermSet anzsrc-for:
157 schema:name Immunology
158 rdf:type schema:DefinedTerm
159 sg:grant.2723975 http://pending.schema.org/fundedItem sg:pub.10.1007/s00262-013-1459-x
160 rdf:type schema:MonetaryGrant
161 sg:grant.2726503 http://pending.schema.org/fundedItem sg:pub.10.1007/s00262-013-1459-x
162 rdf:type schema:MonetaryGrant
163 sg:journal.1096240 schema:issn 0340-7004
164 1432-0851
165 schema:name Cancer Immunology, Immunotherapy
166 rdf:type schema:Periodical
167 sg:person.01107745373.14 schema:affiliation https://www.grid.ac/institutes/grid.413453.4
168 schema:familyName Scheffold
169 schema:givenName Alexander
170 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01107745373.14
171 rdf:type schema:Person
172 sg:person.01137060061.40 schema:affiliation https://www.grid.ac/institutes/grid.420089.7
173 schema:familyName Feldman
174 schema:givenName Steven Alexander
175 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01137060061.40
176 rdf:type schema:Person
177 sg:person.01160641160.49 schema:affiliation https://www.grid.ac/institutes/grid.59409.31
178 schema:familyName Assenmacher
179 schema:givenName Mario
180 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01160641160.49
181 rdf:type schema:Person
182 sg:person.0607775241.72 schema:affiliation https://www.grid.ac/institutes/grid.420089.7
183 schema:familyName Dudley
184 schema:givenName Mark Edward
185 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0607775241.72
186 rdf:type schema:Person
187 sg:person.0717624506.16 schema:affiliation https://www.grid.ac/institutes/grid.420089.7
188 schema:familyName Rosenberg
189 schema:givenName Steven Aaron
190 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0717624506.16
191 rdf:type schema:Person
192 sg:pub.10.1038/44385 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016546162
193 https://doi.org/10.1038/44385
194 rdf:type schema:CreativeWork
195 sg:pub.10.1038/nbt0202-143 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046168734
196 https://doi.org/10.1038/nbt0202-143
197 rdf:type schema:CreativeWork
198 sg:pub.10.1038/nm.1882 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051448639
199 https://doi.org/10.1038/nm.1882
200 rdf:type schema:CreativeWork
201 sg:pub.10.1038/nri1842 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036296603
202 https://doi.org/10.1038/nri1842
203 rdf:type schema:CreativeWork
204 sg:pub.10.1038/nri3191 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013056074
205 https://doi.org/10.1038/nri3191
206 rdf:type schema:CreativeWork
207 https://doi.org/10.1002/eji.200939555 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013787753
208 rdf:type schema:CreativeWork
209 https://doi.org/10.1002/ijc.2910510623 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011270012
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1016/j.coi.2010.01.020 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030001269
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1016/j.immuni.2009.09.014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001113648
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1016/s1535-6108(03)00113-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035078557
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1038/mt.2010.24 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039784712
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1038/mt.2010.272 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053678344
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1056/nejm200010053431407 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002831560
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1056/nejmoa0800251 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050025318
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1056/nejmoa1103849 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009197831
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1073/pnas.0307298101 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038276939
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1073/pnas.0503726102 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030180802
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1073/pnas.0907448106 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011880450
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1073/pnas.1113748109 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012607217
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1073/pnas.92.17.7739 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017993705
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1089/hum.2010.064 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059273502
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1097/00002371-200307000-00005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014162674
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1097/01.cji.0000158855.92792.7a schema:sameAs https://app.dimensions.ai/details/publication/pub.1002151888
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1097/cji.0b013e31819c8bda schema:sameAs https://app.dimensions.ai/details/publication/pub.1028333928
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1126/science.1129003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021480946
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1126/science.272.5270.1939 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062553450
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1126/scitranslmed.3003634 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020921605
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1158/1078-0432.ccr-11-0116 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034962577
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1172/jci24480 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053677825
254 rdf:type schema:CreativeWork
255 https://doi.org/10.1172/jci32103 schema:sameAs https://app.dimensions.ai/details/publication/pub.1063416958
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1172/jci43490 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046210483
258 rdf:type schema:CreativeWork
259 https://doi.org/10.1182/blood-2002-11-3577 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037916496
260 rdf:type schema:CreativeWork
261 https://doi.org/10.1182/blood-2007-09-113050 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040845426
262 rdf:type schema:CreativeWork
263 https://doi.org/10.1182/blood-2008-05-156059 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020008181
264 rdf:type schema:CreativeWork
265 https://doi.org/10.1182/blood-2009-03-211714 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031594949
266 rdf:type schema:CreativeWork
267 https://doi.org/10.1182/blood-2010-04-281931 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033707875
268 rdf:type schema:CreativeWork
269 https://doi.org/10.1182/blood-2010-05-286286 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034635209
270 rdf:type schema:CreativeWork
271 https://doi.org/10.1182/blood-2010-07-294520 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037865345
272 rdf:type schema:CreativeWork
273 https://doi.org/10.1182/blood-2010-10-310599 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038542835
274 rdf:type schema:CreativeWork
275 https://doi.org/10.1200/jco.2010.32.2537 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017008867
276 rdf:type schema:CreativeWork
277 https://doi.org/10.2174/156652311796150390 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069184639
278 rdf:type schema:CreativeWork
279 https://doi.org/10.4049/jimmunol.0902047 schema:sameAs https://app.dimensions.ai/details/publication/pub.1078152676
280 rdf:type schema:CreativeWork
281 https://doi.org/10.4049/jimmunol.175.10.7046 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031627761
282 rdf:type schema:CreativeWork
283 https://doi.org/10.4049/jimmunol.176.12.7726 schema:sameAs https://app.dimensions.ai/details/publication/pub.1077250169
284 rdf:type schema:CreativeWork
285 https://doi.org/10.4049/jimmunol.177.9.6548 schema:sameAs https://app.dimensions.ai/details/publication/pub.1077312039
286 rdf:type schema:CreativeWork
287 https://www.grid.ac/institutes/grid.413453.4 schema:alternateName Leibniz Association
288 schema:name Department of Rheumatology and Clinical Immunology, Charité, Universitätsmedizin Berlin, Charitéplatz 1, 10117, Berlin, Germany
289 German Rheumatism Research Centre (DRFZ) Berlin, Leibniz Association, Berlin, Germany
290 rdf:type schema:Organization
291 https://www.grid.ac/institutes/grid.420089.7 schema:alternateName National Institute of Child Health and Human Development
292 schema:name Surgery Branch, National Cancer Institute, National Institute of Health, 20892, Bethesda, MD, USA
293 rdf:type schema:Organization
294 https://www.grid.ac/institutes/grid.59409.31 schema:alternateName Miltenyi Biotec (Germany)
295 schema:name Research and Development, Miltenyi Biotec GmbH, Bergisch Gladbach, Germany
296 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...