Substitution analog peptide derived from HER-2 can efficiently induce HER-2-specific, HLA-A24 restricted CTLs View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2006-11

AUTHORS

Kousaku Mimura, Koji Kono, Scott Southwood, John Fikes, Akihiro Takahashi, Naoto Miyagawa, Hidemitsu Sugai, Hideki Fujii

ABSTRACT

In order to broaden the possibility for anti-HER-2/neu (HER-2) immune targeting, it is important to identify HLA-A24 restricted peptide epitopes derived from HER-2, since HLA-A24 is one of the most common alleles in Japanese and Asian people. In the present study, we have screened HER-2-derived, HLA-A24 binding peptides for cytotoxic T lymphocyte (CTL) epitopes. A panel of HER-2-derived peptides with HLA-A24 binding motifs and the corresponding analogs designed to enhance HLA-A24 binding affinity were selected. Identification of HER-2-reactive and HLA-A24 restricted CTL epitopes were performed by a reverse immunology approach. To induce HER-2-reactive and HLA-A24 restricted CTLs, PBMCs from healthy donors were repeatedly stimulated with monocytes-derived, mature DCs pulsed with HER-2 peptide. Subsequent peptide-induced T cells were tested for the specificity by enzyme linked immunospot, cytotoxicity and tetramer assays. CTL clones were then obtained from the CTL lines by limiting dilution. Of the peptides containing HLA-A24 binding motifs, 16 peptides (nine mers) including wild type peptides (IC50 <1,000 nM) and substituted analog peptides (IC50 <50 nM) were selected for the present study. Our studies show that an analog peptide, HER-2(905AA), derived from HER-2(905) could efficiently induce HER-2-reactive and HLA-A24 restricted CTLs. The reactivity of the HER-2(905AA)-induced CTL (CTL905AA) was confirmed by different CTL assays. The CTL905AA clones also were able to lyse HER-2(+), HLA-A24(+) tumor cells and cytotoxicity could be significantly reduced in cold target inhibition assays using cold targets pulsed with the HER-2(905) wild type peptide as well as the inducing HER-2(905AA) analog peptide. A newly identified HER-2(905) peptide epitope is naturally processed and presented as a CTL epitope on HER-2 overexpressing tumor cells, and an MHC anchor-substituted analog, HER-2(905AA), can efficiently induce HER-2-specific, HLA-A24 restricted CTLs. More... »

PAGES

1358-1366

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00262-006-0123-0

DOI

http://dx.doi.org/10.1007/s00262-006-0123-0

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1030792908

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/16435129


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1107", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Immunology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Alleles", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antigens, Neoplasm", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Line, Tumor", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cytotoxicity, Immunologic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Dendritic Cells", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Epitopes, T-Lymphocyte", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "HLA-A Antigens", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "HLA-A24 Antigen", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Inhibitory Concentration 50", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Leukocytes, Mononuclear", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Peptides", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Receptor, ErbB-2", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "T-Lymphocytes, Cytotoxic", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Yamanashi", 
          "id": "https://www.grid.ac/institutes/grid.267500.6", 
          "name": [
            "First Department of Surgery, University of Yamanashi, 1110 Tamaho, 409-3898, Yamanashi, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mimura", 
        "givenName": "Kousaku", 
        "id": "sg:person.01033220160.97", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01033220160.97"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Yamanashi", 
          "id": "https://www.grid.ac/institutes/grid.267500.6", 
          "name": [
            "First Department of Surgery, University of Yamanashi, 1110 Tamaho, 409-3898, Yamanashi, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kono", 
        "givenName": "Koji", 
        "id": "sg:person.01362133435.33", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01362133435.33"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Epimmune, Inc., 92121, San Diego, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Southwood", 
        "givenName": "Scott", 
        "id": "sg:person.0736656633.97", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0736656633.97"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Epimmune, Inc., 92121, San Diego, CA, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Fikes", 
        "givenName": "John", 
        "id": "sg:person.0650035240.22", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0650035240.22"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Yamanashi", 
          "id": "https://www.grid.ac/institutes/grid.267500.6", 
          "name": [
            "First Department of Surgery, University of Yamanashi, 1110 Tamaho, 409-3898, Yamanashi, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Takahashi", 
        "givenName": "Akihiro", 
        "id": "sg:person.0732251172.76", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0732251172.76"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Yamanashi", 
          "id": "https://www.grid.ac/institutes/grid.267500.6", 
          "name": [
            "First Department of Surgery, University of Yamanashi, 1110 Tamaho, 409-3898, Yamanashi, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Miyagawa", 
        "givenName": "Naoto", 
        "id": "sg:person.01215561760.26", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01215561760.26"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Yamanashi", 
          "id": "https://www.grid.ac/institutes/grid.267500.6", 
          "name": [
            "First Department of Surgery, University of Yamanashi, 1110 Tamaho, 409-3898, Yamanashi, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sugai", 
        "givenName": "Hidemitsu", 
        "id": "sg:person.01015045552.72", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01015045552.72"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Yamanashi", 
          "id": "https://www.grid.ac/institutes/grid.267500.6", 
          "name": [
            "First Department of Surgery, University of Yamanashi, 1110 Tamaho, 409-3898, Yamanashi, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Fujii", 
        "givenName": "Hideki", 
        "id": "sg:person.014173406752.52", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014173406752.52"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1056/nejm198812223192527", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001852342"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nm1295-1297", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003113140", 
          "https://doi.org/10.1038/nm1295-1297"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1054/bjoc.2000.1547", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006250216", 
          "https://doi.org/10.1054/bjoc.2000.1547"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(93)90472-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006443560"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/1521-4141(200011)30:11<3338::aid-immu3338>3.0.co;2-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007747472"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/1097-0215(20000815)87:4<553::aid-ijc15>3.0.co;2-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014996107"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.91.6.2105", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015460123"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/(sici)1097-0215(19970106)70:1<112::aid-ijc17>3.0.co;2-n", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017409608"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4049/jimmunol.165.2.948", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017957829"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/ijc.1508", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018118873"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.180.1.347", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023959186"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0198-8859(97)00255-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026346952"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.178.2.489", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033051074"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/ijc.10179", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033329756"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.181.6.2109", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041879226"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.180.1.35", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045042533"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/ijc.10257", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045202646"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.176.5.1453", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045431536"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/(sici)1097-0215(19981005)78:2<202::aid-ijc14>3.0.co;2-c", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046885808"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.91.14.6458", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049481800"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1399-0039.1996.tb02520.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052814994"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.2470152", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062539373"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.2999974", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062580096"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.7513441", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062647396"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074487446", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4049/jimmunol.167.2.787", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1074845547"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1075189856", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082427399", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082427421", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082473611", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082671976", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082960813", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083044482", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083141937", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083213112", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2006-11", 
    "datePublishedReg": "2006-11-01", 
    "description": "In order to broaden the possibility for anti-HER-2/neu (HER-2) immune targeting, it is important to identify HLA-A24 restricted peptide epitopes derived from HER-2, since HLA-A24 is one of the most common alleles in Japanese and Asian people. In the present study, we have screened HER-2-derived, HLA-A24 binding peptides for cytotoxic T lymphocyte (CTL) epitopes. A panel of HER-2-derived peptides with HLA-A24 binding motifs and the corresponding analogs designed to enhance HLA-A24 binding affinity were selected. Identification of HER-2-reactive and HLA-A24 restricted CTL epitopes were performed by a reverse immunology approach. To induce HER-2-reactive and HLA-A24 restricted CTLs, PBMCs from healthy donors were repeatedly stimulated with monocytes-derived, mature DCs pulsed with HER-2 peptide. Subsequent peptide-induced T cells were tested for the specificity by enzyme linked immunospot, cytotoxicity and tetramer assays. CTL clones were then obtained from the CTL lines by limiting dilution. Of the peptides containing HLA-A24 binding motifs, 16 peptides (nine mers) including wild type peptides (IC50 <1,000 nM) and substituted analog peptides (IC50 <50 nM) were selected for the present study. Our studies show that an analog peptide, HER-2(905AA), derived from HER-2(905) could efficiently induce HER-2-reactive and HLA-A24 restricted CTLs. The reactivity of the HER-2(905AA)-induced CTL (CTL905AA) was confirmed by different CTL assays. The CTL905AA clones also were able to lyse HER-2(+), HLA-A24(+) tumor cells and cytotoxicity could be significantly reduced in cold target inhibition assays using cold targets pulsed with the HER-2(905) wild type peptide as well as the inducing HER-2(905AA) analog peptide. A newly identified HER-2(905) peptide epitope is naturally processed and presented as a CTL epitope on HER-2 overexpressing tumor cells, and an MHC anchor-substituted analog, HER-2(905AA), can efficiently induce HER-2-specific, HLA-A24 restricted CTLs.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s00262-006-0123-0", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1096240", 
        "issn": [
          "0340-7004", 
          "1432-0851"
        ], 
        "name": "Cancer Immunology, Immunotherapy", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "11", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "55"
      }
    ], 
    "name": "Substitution analog peptide derived from HER-2 can efficiently induce HER-2-specific, HLA-A24 restricted CTLs", 
    "pagination": "1358-1366", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "92b1878e27c3e0e1523b1b1c1cc3f495ea5092171b22b0552c3fecd3ee8b1ca3"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "16435129"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "8605732"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00262-006-0123-0"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1030792908"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00262-006-0123-0", 
      "https://app.dimensions.ai/details/publication/pub.1030792908"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T12:36", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000363_0000000363/records_70031_00000001.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007%2Fs00262-006-0123-0"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00262-006-0123-0'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00262-006-0123-0'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00262-006-0123-0'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00262-006-0123-0'


 

This table displays all metadata directly associated to this object as RDF triples.

275 TRIPLES      21 PREDICATES      78 URIs      35 LITERALS      23 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00262-006-0123-0 schema:about N07b06cba7af945a2b95c746bb57f0333
2 N1dc01d8c12ab4034944b7fbab55247d7
3 N38583591af6142459ad34136746a2dde
4 N672af64d44cc4f3a851c66f7f44a3893
5 N8297f193d1c9489eb8e6b1ed84e2e6a8
6 N8eedb4fb7f9247e0b0f57be1512cad9a
7 Na555c77396cd4a10af8d67d271d91fe5
8 Nb94c350f61fc4405904fe172a349b10a
9 Nccb2605476714d8ab59f3dd3d48a6e8f
10 Ncd20ee8cd000416ab89b4ad3f3674888
11 Nd6a183b2e5d54ecab35085c3d552d543
12 Nd86bf5bffc434d9d9879120852479328
13 Neb2dc6fdd5a84f78a900724f2731a90d
14 Nf8d0841d78614c15bc25c363a1832972
15 anzsrc-for:11
16 anzsrc-for:1107
17 schema:author Na4a2623defaa4b259213926d99028f9e
18 schema:citation sg:pub.10.1038/nm1295-1297
19 sg:pub.10.1054/bjoc.2000.1547
20 https://app.dimensions.ai/details/publication/pub.1074487446
21 https://app.dimensions.ai/details/publication/pub.1075189856
22 https://app.dimensions.ai/details/publication/pub.1082427399
23 https://app.dimensions.ai/details/publication/pub.1082427421
24 https://app.dimensions.ai/details/publication/pub.1082473611
25 https://app.dimensions.ai/details/publication/pub.1082671976
26 https://app.dimensions.ai/details/publication/pub.1082960813
27 https://app.dimensions.ai/details/publication/pub.1083044482
28 https://app.dimensions.ai/details/publication/pub.1083141937
29 https://app.dimensions.ai/details/publication/pub.1083213112
30 https://doi.org/10.1002/(sici)1097-0215(19970106)70:1<112::aid-ijc17>3.0.co;2-n
31 https://doi.org/10.1002/(sici)1097-0215(19981005)78:2<202::aid-ijc14>3.0.co;2-c
32 https://doi.org/10.1002/1097-0215(20000815)87:4<553::aid-ijc15>3.0.co;2-8
33 https://doi.org/10.1002/1521-4141(200011)30:11<3338::aid-immu3338>3.0.co;2-3
34 https://doi.org/10.1002/ijc.10179
35 https://doi.org/10.1002/ijc.10257
36 https://doi.org/10.1002/ijc.1508
37 https://doi.org/10.1016/0092-8674(93)90472-3
38 https://doi.org/10.1016/s0198-8859(97)00255-3
39 https://doi.org/10.1056/nejm198812223192527
40 https://doi.org/10.1073/pnas.91.14.6458
41 https://doi.org/10.1073/pnas.91.6.2105
42 https://doi.org/10.1084/jem.176.5.1453
43 https://doi.org/10.1084/jem.178.2.489
44 https://doi.org/10.1084/jem.180.1.347
45 https://doi.org/10.1084/jem.180.1.35
46 https://doi.org/10.1084/jem.181.6.2109
47 https://doi.org/10.1111/j.1399-0039.1996.tb02520.x
48 https://doi.org/10.1126/science.2470152
49 https://doi.org/10.1126/science.2999974
50 https://doi.org/10.1126/science.7513441
51 https://doi.org/10.4049/jimmunol.165.2.948
52 https://doi.org/10.4049/jimmunol.167.2.787
53 schema:datePublished 2006-11
54 schema:datePublishedReg 2006-11-01
55 schema:description In order to broaden the possibility for anti-HER-2/neu (HER-2) immune targeting, it is important to identify HLA-A24 restricted peptide epitopes derived from HER-2, since HLA-A24 is one of the most common alleles in Japanese and Asian people. In the present study, we have screened HER-2-derived, HLA-A24 binding peptides for cytotoxic T lymphocyte (CTL) epitopes. A panel of HER-2-derived peptides with HLA-A24 binding motifs and the corresponding analogs designed to enhance HLA-A24 binding affinity were selected. Identification of HER-2-reactive and HLA-A24 restricted CTL epitopes were performed by a reverse immunology approach. To induce HER-2-reactive and HLA-A24 restricted CTLs, PBMCs from healthy donors were repeatedly stimulated with monocytes-derived, mature DCs pulsed with HER-2 peptide. Subsequent peptide-induced T cells were tested for the specificity by enzyme linked immunospot, cytotoxicity and tetramer assays. CTL clones were then obtained from the CTL lines by limiting dilution. Of the peptides containing HLA-A24 binding motifs, 16 peptides (nine mers) including wild type peptides (IC50 <1,000 nM) and substituted analog peptides (IC50 <50 nM) were selected for the present study. Our studies show that an analog peptide, HER-2(905AA), derived from HER-2(905) could efficiently induce HER-2-reactive and HLA-A24 restricted CTLs. The reactivity of the HER-2(905AA)-induced CTL (CTL905AA) was confirmed by different CTL assays. The CTL905AA clones also were able to lyse HER-2(+), HLA-A24(+) tumor cells and cytotoxicity could be significantly reduced in cold target inhibition assays using cold targets pulsed with the HER-2(905) wild type peptide as well as the inducing HER-2(905AA) analog peptide. A newly identified HER-2(905) peptide epitope is naturally processed and presented as a CTL epitope on HER-2 overexpressing tumor cells, and an MHC anchor-substituted analog, HER-2(905AA), can efficiently induce HER-2-specific, HLA-A24 restricted CTLs.
56 schema:genre research_article
57 schema:inLanguage en
58 schema:isAccessibleForFree false
59 schema:isPartOf N2fec2262056b41e790aa94cf011f97fc
60 Nede2dfdba960480aa5485ff3cd74bc8a
61 sg:journal.1096240
62 schema:name Substitution analog peptide derived from HER-2 can efficiently induce HER-2-specific, HLA-A24 restricted CTLs
63 schema:pagination 1358-1366
64 schema:productId N25eec1d6331e4e71b32035ce45b8e4fc
65 N31a42ea8c0ab4e60a38e2b7b0323e92c
66 N69d47fcd66c0499f9fce28540942582e
67 N995446551c0642ff83cee46c9b00f218
68 N9a836c69166b495aabe0b1b6cbb2ef56
69 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030792908
70 https://doi.org/10.1007/s00262-006-0123-0
71 schema:sdDatePublished 2019-04-11T12:36
72 schema:sdLicense https://scigraph.springernature.com/explorer/license/
73 schema:sdPublisher Ne673a75e089f433792f9a30a968e5495
74 schema:url http://link.springer.com/10.1007%2Fs00262-006-0123-0
75 sgo:license sg:explorer/license/
76 sgo:sdDataset articles
77 rdf:type schema:ScholarlyArticle
78 N07b06cba7af945a2b95c746bb57f0333 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
79 schema:name Leukocytes, Mononuclear
80 rdf:type schema:DefinedTerm
81 N0849c9c0add24521bd0dfbf79a309ef5 rdf:first sg:person.014173406752.52
82 rdf:rest rdf:nil
83 N0d80068cb7424212a752b99897f2d1ac rdf:first sg:person.0736656633.97
84 rdf:rest N34d527c0a7764562ba40517a89c07445
85 N1dc01d8c12ab4034944b7fbab55247d7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
86 schema:name Peptides
87 rdf:type schema:DefinedTerm
88 N25eec1d6331e4e71b32035ce45b8e4fc schema:name doi
89 schema:value 10.1007/s00262-006-0123-0
90 rdf:type schema:PropertyValue
91 N294dd99d6f1e4e149a6e9f783b3dd5cb rdf:first sg:person.01362133435.33
92 rdf:rest N0d80068cb7424212a752b99897f2d1ac
93 N2fec2262056b41e790aa94cf011f97fc schema:volumeNumber 55
94 rdf:type schema:PublicationVolume
95 N31a42ea8c0ab4e60a38e2b7b0323e92c schema:name readcube_id
96 schema:value 92b1878e27c3e0e1523b1b1c1cc3f495ea5092171b22b0552c3fecd3ee8b1ca3
97 rdf:type schema:PropertyValue
98 N34d527c0a7764562ba40517a89c07445 rdf:first sg:person.0650035240.22
99 rdf:rest N5840e558e1d3438ca8a870c157bb8979
100 N38583591af6142459ad34136746a2dde schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
101 schema:name Cytotoxicity, Immunologic
102 rdf:type schema:DefinedTerm
103 N5840e558e1d3438ca8a870c157bb8979 rdf:first sg:person.0732251172.76
104 rdf:rest Nfeccb93c51184c37b54760e54f4dc3c7
105 N672af64d44cc4f3a851c66f7f44a3893 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
106 schema:name HLA-A Antigens
107 rdf:type schema:DefinedTerm
108 N69d47fcd66c0499f9fce28540942582e schema:name pubmed_id
109 schema:value 16435129
110 rdf:type schema:PropertyValue
111 N70237148835c4e1ba679b51518e794da rdf:first sg:person.01015045552.72
112 rdf:rest N0849c9c0add24521bd0dfbf79a309ef5
113 N788ca328b0d24ba990bf0a26c342faa6 schema:name Epimmune, Inc., 92121, San Diego, CA, USA
114 rdf:type schema:Organization
115 N8297f193d1c9489eb8e6b1ed84e2e6a8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
116 schema:name Dendritic Cells
117 rdf:type schema:DefinedTerm
118 N8eedb4fb7f9247e0b0f57be1512cad9a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
119 schema:name Humans
120 rdf:type schema:DefinedTerm
121 N98e1363cbde14da9ab07ba4682b76b9f schema:name Epimmune, Inc., 92121, San Diego, CA, USA
122 rdf:type schema:Organization
123 N995446551c0642ff83cee46c9b00f218 schema:name nlm_unique_id
124 schema:value 8605732
125 rdf:type schema:PropertyValue
126 N9a836c69166b495aabe0b1b6cbb2ef56 schema:name dimensions_id
127 schema:value pub.1030792908
128 rdf:type schema:PropertyValue
129 Na4a2623defaa4b259213926d99028f9e rdf:first sg:person.01033220160.97
130 rdf:rest N294dd99d6f1e4e149a6e9f783b3dd5cb
131 Na555c77396cd4a10af8d67d271d91fe5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
132 schema:name Antigens, Neoplasm
133 rdf:type schema:DefinedTerm
134 Nb94c350f61fc4405904fe172a349b10a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
135 schema:name Receptor, ErbB-2
136 rdf:type schema:DefinedTerm
137 Nccb2605476714d8ab59f3dd3d48a6e8f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
138 schema:name Cell Line, Tumor
139 rdf:type schema:DefinedTerm
140 Ncd20ee8cd000416ab89b4ad3f3674888 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
141 schema:name T-Lymphocytes, Cytotoxic
142 rdf:type schema:DefinedTerm
143 Nd6a183b2e5d54ecab35085c3d552d543 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
144 schema:name Epitopes, T-Lymphocyte
145 rdf:type schema:DefinedTerm
146 Nd86bf5bffc434d9d9879120852479328 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
147 schema:name Inhibitory Concentration 50
148 rdf:type schema:DefinedTerm
149 Ne673a75e089f433792f9a30a968e5495 schema:name Springer Nature - SN SciGraph project
150 rdf:type schema:Organization
151 Neb2dc6fdd5a84f78a900724f2731a90d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
152 schema:name HLA-A24 Antigen
153 rdf:type schema:DefinedTerm
154 Nede2dfdba960480aa5485ff3cd74bc8a schema:issueNumber 11
155 rdf:type schema:PublicationIssue
156 Nf8d0841d78614c15bc25c363a1832972 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
157 schema:name Alleles
158 rdf:type schema:DefinedTerm
159 Nfeccb93c51184c37b54760e54f4dc3c7 rdf:first sg:person.01215561760.26
160 rdf:rest N70237148835c4e1ba679b51518e794da
161 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
162 schema:name Medical and Health Sciences
163 rdf:type schema:DefinedTerm
164 anzsrc-for:1107 schema:inDefinedTermSet anzsrc-for:
165 schema:name Immunology
166 rdf:type schema:DefinedTerm
167 sg:journal.1096240 schema:issn 0340-7004
168 1432-0851
169 schema:name Cancer Immunology, Immunotherapy
170 rdf:type schema:Periodical
171 sg:person.01015045552.72 schema:affiliation https://www.grid.ac/institutes/grid.267500.6
172 schema:familyName Sugai
173 schema:givenName Hidemitsu
174 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01015045552.72
175 rdf:type schema:Person
176 sg:person.01033220160.97 schema:affiliation https://www.grid.ac/institutes/grid.267500.6
177 schema:familyName Mimura
178 schema:givenName Kousaku
179 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01033220160.97
180 rdf:type schema:Person
181 sg:person.01215561760.26 schema:affiliation https://www.grid.ac/institutes/grid.267500.6
182 schema:familyName Miyagawa
183 schema:givenName Naoto
184 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01215561760.26
185 rdf:type schema:Person
186 sg:person.01362133435.33 schema:affiliation https://www.grid.ac/institutes/grid.267500.6
187 schema:familyName Kono
188 schema:givenName Koji
189 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01362133435.33
190 rdf:type schema:Person
191 sg:person.014173406752.52 schema:affiliation https://www.grid.ac/institutes/grid.267500.6
192 schema:familyName Fujii
193 schema:givenName Hideki
194 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014173406752.52
195 rdf:type schema:Person
196 sg:person.0650035240.22 schema:affiliation N98e1363cbde14da9ab07ba4682b76b9f
197 schema:familyName Fikes
198 schema:givenName John
199 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0650035240.22
200 rdf:type schema:Person
201 sg:person.0732251172.76 schema:affiliation https://www.grid.ac/institutes/grid.267500.6
202 schema:familyName Takahashi
203 schema:givenName Akihiro
204 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0732251172.76
205 rdf:type schema:Person
206 sg:person.0736656633.97 schema:affiliation N788ca328b0d24ba990bf0a26c342faa6
207 schema:familyName Southwood
208 schema:givenName Scott
209 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0736656633.97
210 rdf:type schema:Person
211 sg:pub.10.1038/nm1295-1297 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003113140
212 https://doi.org/10.1038/nm1295-1297
213 rdf:type schema:CreativeWork
214 sg:pub.10.1054/bjoc.2000.1547 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006250216
215 https://doi.org/10.1054/bjoc.2000.1547
216 rdf:type schema:CreativeWork
217 https://app.dimensions.ai/details/publication/pub.1074487446 schema:CreativeWork
218 https://app.dimensions.ai/details/publication/pub.1075189856 schema:CreativeWork
219 https://app.dimensions.ai/details/publication/pub.1082427399 schema:CreativeWork
220 https://app.dimensions.ai/details/publication/pub.1082427421 schema:CreativeWork
221 https://app.dimensions.ai/details/publication/pub.1082473611 schema:CreativeWork
222 https://app.dimensions.ai/details/publication/pub.1082671976 schema:CreativeWork
223 https://app.dimensions.ai/details/publication/pub.1082960813 schema:CreativeWork
224 https://app.dimensions.ai/details/publication/pub.1083044482 schema:CreativeWork
225 https://app.dimensions.ai/details/publication/pub.1083141937 schema:CreativeWork
226 https://app.dimensions.ai/details/publication/pub.1083213112 schema:CreativeWork
227 https://doi.org/10.1002/(sici)1097-0215(19970106)70:1<112::aid-ijc17>3.0.co;2-n schema:sameAs https://app.dimensions.ai/details/publication/pub.1017409608
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1002/(sici)1097-0215(19981005)78:2<202::aid-ijc14>3.0.co;2-c schema:sameAs https://app.dimensions.ai/details/publication/pub.1046885808
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1002/1097-0215(20000815)87:4<553::aid-ijc15>3.0.co;2-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014996107
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1002/1521-4141(200011)30:11<3338::aid-immu3338>3.0.co;2-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007747472
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1002/ijc.10179 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033329756
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1002/ijc.10257 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045202646
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1002/ijc.1508 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018118873
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1016/0092-8674(93)90472-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006443560
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1016/s0198-8859(97)00255-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026346952
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1056/nejm198812223192527 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001852342
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1073/pnas.91.14.6458 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049481800
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1073/pnas.91.6.2105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015460123
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1084/jem.176.5.1453 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045431536
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1084/jem.178.2.489 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033051074
254 rdf:type schema:CreativeWork
255 https://doi.org/10.1084/jem.180.1.347 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023959186
256 rdf:type schema:CreativeWork
257 https://doi.org/10.1084/jem.180.1.35 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045042533
258 rdf:type schema:CreativeWork
259 https://doi.org/10.1084/jem.181.6.2109 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041879226
260 rdf:type schema:CreativeWork
261 https://doi.org/10.1111/j.1399-0039.1996.tb02520.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1052814994
262 rdf:type schema:CreativeWork
263 https://doi.org/10.1126/science.2470152 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062539373
264 rdf:type schema:CreativeWork
265 https://doi.org/10.1126/science.2999974 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062580096
266 rdf:type schema:CreativeWork
267 https://doi.org/10.1126/science.7513441 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062647396
268 rdf:type schema:CreativeWork
269 https://doi.org/10.4049/jimmunol.165.2.948 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017957829
270 rdf:type schema:CreativeWork
271 https://doi.org/10.4049/jimmunol.167.2.787 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074845547
272 rdf:type schema:CreativeWork
273 https://www.grid.ac/institutes/grid.267500.6 schema:alternateName University of Yamanashi
274 schema:name First Department of Surgery, University of Yamanashi, 1110 Tamaho, 409-3898, Yamanashi, Japan
275 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...