T-cell-mediated lysis of B cells induced by a CD19xCD3 bispecific single-chain antibody is perforin dependent and death receptor independent View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2004-07

AUTHORS

Michael Gruen, Kurt Bommert, Ralf C. Bargou

ABSTRACT

A recently developed bispecific antibody construct, directed against CD19 and CD3 (bscCD19xCD3), induces T-cell-mediated lysis of allogeneic and autologous B cells in a specific and highly efficient manner. Since knowledge of the molecular mechanisms underlying this lysis is limited, a study on bscCD19xCD3-activated T-cell-effector pathways was performed. BscCD19xCD3-induced lysis of target B-cell lines Nalm-6, Daudi, and Raji and of autologous primary B cells is caused by the perforin-dependent granule-exocytosis pathway but not by the death ligands FasL, TRAIL, or TNF-alpha. When activated by bscCD19xCD3 and Raji cells, T cells express FasL mRNA, but incubation of Raji cells with cell-free supernatants from cytotoxicity experiments caused an upregulation of c-Flipl, possibly accounting for the cells' insensitivity toward death-receptor-mediated lysis. In addition to granule exocytosis, Raji cells are lysed by at least one mechanism independent of perforin, which requires transport through the T cell's Golgi apparatus. More... »

PAGES

625-632

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00262-003-0496-2

DOI

http://dx.doi.org/10.1007/s00262-003-0496-2

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1034809523

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/15175907


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antibodies, Bispecific", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antigens, CD19", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Apoptosis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Apoptosis Regulatory Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "B-Lymphocytes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "CASP8 and FADD-Like Apoptosis Regulating Protein", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "CD3 Complex", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Carrier Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cytoplasmic Granules", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cytotoxicity, Immunologic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Exocytosis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fas Ligand Protein", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Humans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Immunotherapy", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Intracellular Signaling Peptides and Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Ligands", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Lymphocyte Activation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Membrane Glycoproteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Perforin", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Pore Forming Cytotoxic Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "T-Lymphocytes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "T-Lymphocytes, Cytotoxic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "TNF-Related Apoptosis-Inducing Ligand", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tumor Cells, Cultured", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tumor Necrosis Factor-alpha", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Up-Regulation", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Friedrich Schiller University Jena", 
          "id": "https://www.grid.ac/institutes/grid.9613.d", 
          "name": [
            "Department of Hematology, Oncology and Tumorimmunology, Robert R\u00f6ssle Clinic, Charit\u00e9, Humboldt University of Berlin, Berlin, Germany", 
            "Max Delbr\u00fcck Center for Molecular Medicine, Robert-R\u00f6ssle-Str. 10, 13125, Berlin, Germany", 
            "Research Unit Molecular Cell Biology, University of Jena, Jena, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gruen", 
        "givenName": "Michael", 
        "id": "sg:person.01253507773.99", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01253507773.99"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Max Delbr\u00fcck Center for Molecular Medicine", 
          "id": "https://www.grid.ac/institutes/grid.419491.0", 
          "name": [
            "Max Delbr\u00fcck Center for Molecular Medicine, Robert-R\u00f6ssle-Str. 10, 13125, Berlin, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bommert", 
        "givenName": "Kurt", 
        "id": "sg:person.01200355130.11", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01200355130.11"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Helios Hospital Berlin-Buch", 
          "id": "https://www.grid.ac/institutes/grid.491869.b", 
          "name": [
            "Department of Hematology, Oncology and Tumorimmunology, Robert R\u00f6ssle Clinic, Charit\u00e9, Humboldt University of Berlin, Berlin, Germany", 
            "Max Delbr\u00fcck Center for Molecular Medicine, Robert-R\u00f6ssle-Str. 10, 13125, Berlin, Germany", 
            "Helios Klinikum Berlin-Buch, Berlin, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bargou", 
        "givenName": "Ralf C.", 
        "id": "sg:person.01146675164.26", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01146675164.26"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/sj.leu.2402890", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000138204", 
          "https://doi.org/10.1038/sj.leu.2402890"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.leu.2402890", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000138204", 
          "https://doi.org/10.1038/sj.leu.2402890"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0167-5699(00)01659-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001971615"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1084/jem.175.2.553", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005248559"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/ijc.10557", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005681080"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35037728", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022325441", 
          "https://doi.org/10.1038/35037728"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/35037728", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022325441", 
          "https://doi.org/10.1038/35037728"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/(sici)1097-0215(19990301)80:5<715::aid-ijc15>3.0.co;2-p", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026331307"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0952-7915(00)00094-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031996667"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.271.46.29073", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034054657"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.cr.7290053", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034520577", 
          "https://doi.org/10.1038/sj.cr.7290053"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/sj.cr.7290053", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034520577", 
          "https://doi.org/10.1038/sj.cr.7290053"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1182/blood.v98.5.1326", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040463948"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s002620050357", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042485673", 
          "https://doi.org/10.1007/s002620050357"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3322/canjclin.49.1.8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044953296"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0171-2985(97)80062-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047053183"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4049/jimmunol.170.8.4397", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049032729"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.immunol.20.100201.131730", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052629241"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/jnci/85.12.1003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059817975"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074262614", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074361775", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074525887", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074601318", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074610551", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4049/jimmunol.165.6.3023", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1074694833"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4049/jimmunol.165.8.4405", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1074713293"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074889443", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074999528", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082882712", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083072553", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2004-07", 
    "datePublishedReg": "2004-07-01", 
    "description": "A recently developed bispecific antibody construct, directed against CD19 and CD3 (bscCD19xCD3), induces T-cell-mediated lysis of allogeneic and autologous B cells in a specific and highly efficient manner. Since knowledge of the molecular mechanisms underlying this lysis is limited, a study on bscCD19xCD3-activated T-cell-effector pathways was performed. BscCD19xCD3-induced lysis of target B-cell lines Nalm-6, Daudi, and Raji and of autologous primary B cells is caused by the perforin-dependent granule-exocytosis pathway but not by the death ligands FasL, TRAIL, or TNF-alpha. When activated by bscCD19xCD3 and Raji cells, T cells express FasL mRNA, but incubation of Raji cells with cell-free supernatants from cytotoxicity experiments caused an upregulation of c-Flipl, possibly accounting for the cells' insensitivity toward death-receptor-mediated lysis. In addition to granule exocytosis, Raji cells are lysed by at least one mechanism independent of perforin, which requires transport through the T cell's Golgi apparatus.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s00262-003-0496-2", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1096240", 
        "issn": [
          "0340-7004", 
          "1432-0851"
        ], 
        "name": "Cancer Immunology, Immunotherapy", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "7", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "53"
      }
    ], 
    "name": "T-cell-mediated lysis of B cells induced by a CD19xCD3 bispecific single-chain antibody is perforin dependent and death receptor independent", 
    "pagination": "625-632", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "a5d1b6aea866c78633610fe73211f9bf62fd33f005977c18fe92858d00d02ac7"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "15175907"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "8605732"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00262-003-0496-2"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1034809523"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00262-003-0496-2", 
      "https://app.dimensions.ai/details/publication/pub.1034809523"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T15:05", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8663_00000533.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007%2Fs00262-003-0496-2"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00262-003-0496-2'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00262-003-0496-2'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00262-003-0496-2'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00262-003-0496-2'


 

This table displays all metadata directly associated to this object as RDF triples.

273 TRIPLES      21 PREDICATES      82 URIs      47 LITERALS      35 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00262-003-0496-2 schema:about N1d5c5b56f3d94e3cbb2b7d9ab1e7a8fe
2 N31419f7d4ada44f5abb089f4ebc8b250
3 N4e9191cbd40148c082deb3fe2a27e299
4 N5a63a0e221284e36b6cd6b968613af02
5 N5c5dee8135504e1f8ae81c7eafa541d3
6 N5d6ea036c986428baee5d0867e54f88a
7 N63f5e3aa19fc48139bd746f9e5b7a325
8 N683bc8922b1942459d333341ba2f6b92
9 N6b134474d36a42b980a7113c92e6d724
10 N6ed17a1a215445b2ae05bcbb2e9092fe
11 N72f43b10f1134670ad15b7a9b7181e4f
12 N7cd30087efa4431aad4b6654db05ca2e
13 N86e033eb50d94d089c39c30f4ffe6ae5
14 N9523a052b6a547cabc00941d14e2566e
15 N99a6c40d8cab418ebfe7727c5f846ef4
16 Na3b39b6486984a2a87bb23597ddaa7a9
17 Nae34fbfca9a74ea59c74201d94889139
18 Nb1f6a6764cb94fedb3cba2ed973bafd3
19 Nbc46798a5dd64099a76ba81898be6cd1
20 Nbfb02dcd26b1455f809e1578da2eaffd
21 Nc06133bdab5c42888d5676eebeddd286
22 Nd8f0131000e146828329aff7cfbffc0d
23 Nea306575595546a392c9e73552550fea
24 Neb141849b95a4a0696c7be6726264c52
25 Nf0f143d0d8be4f12a097fe3a63e7e163
26 Nf44da7989089448f825e4553252971a0
27 anzsrc-for:06
28 anzsrc-for:0601
29 schema:author N801d3fbb37134cd9a146e0666de280d3
30 schema:citation sg:pub.10.1007/s002620050357
31 sg:pub.10.1038/35037728
32 sg:pub.10.1038/sj.cr.7290053
33 sg:pub.10.1038/sj.leu.2402890
34 https://app.dimensions.ai/details/publication/pub.1074262614
35 https://app.dimensions.ai/details/publication/pub.1074361775
36 https://app.dimensions.ai/details/publication/pub.1074525887
37 https://app.dimensions.ai/details/publication/pub.1074601318
38 https://app.dimensions.ai/details/publication/pub.1074610551
39 https://app.dimensions.ai/details/publication/pub.1074889443
40 https://app.dimensions.ai/details/publication/pub.1074999528
41 https://app.dimensions.ai/details/publication/pub.1082882712
42 https://app.dimensions.ai/details/publication/pub.1083072553
43 https://doi.org/10.1002/(sici)1097-0215(19990301)80:5<715::aid-ijc15>3.0.co;2-p
44 https://doi.org/10.1002/ijc.10557
45 https://doi.org/10.1016/s0167-5699(00)01659-5
46 https://doi.org/10.1016/s0171-2985(97)80062-9
47 https://doi.org/10.1016/s0952-7915(00)00094-7
48 https://doi.org/10.1074/jbc.271.46.29073
49 https://doi.org/10.1084/jem.175.2.553
50 https://doi.org/10.1093/jnci/85.12.1003
51 https://doi.org/10.1146/annurev.immunol.20.100201.131730
52 https://doi.org/10.1182/blood.v98.5.1326
53 https://doi.org/10.3322/canjclin.49.1.8
54 https://doi.org/10.4049/jimmunol.165.6.3023
55 https://doi.org/10.4049/jimmunol.165.8.4405
56 https://doi.org/10.4049/jimmunol.170.8.4397
57 schema:datePublished 2004-07
58 schema:datePublishedReg 2004-07-01
59 schema:description A recently developed bispecific antibody construct, directed against CD19 and CD3 (bscCD19xCD3), induces T-cell-mediated lysis of allogeneic and autologous B cells in a specific and highly efficient manner. Since knowledge of the molecular mechanisms underlying this lysis is limited, a study on bscCD19xCD3-activated T-cell-effector pathways was performed. BscCD19xCD3-induced lysis of target B-cell lines Nalm-6, Daudi, and Raji and of autologous primary B cells is caused by the perforin-dependent granule-exocytosis pathway but not by the death ligands FasL, TRAIL, or TNF-alpha. When activated by bscCD19xCD3 and Raji cells, T cells express FasL mRNA, but incubation of Raji cells with cell-free supernatants from cytotoxicity experiments caused an upregulation of c-Flipl, possibly accounting for the cells' insensitivity toward death-receptor-mediated lysis. In addition to granule exocytosis, Raji cells are lysed by at least one mechanism independent of perforin, which requires transport through the T cell's Golgi apparatus.
60 schema:genre research_article
61 schema:inLanguage en
62 schema:isAccessibleForFree false
63 schema:isPartOf N022aebcd18c14c01b8cff21a1d55e9ae
64 Naa21ac8aacf546f486e1579e5d202c76
65 sg:journal.1096240
66 schema:name T-cell-mediated lysis of B cells induced by a CD19xCD3 bispecific single-chain antibody is perforin dependent and death receptor independent
67 schema:pagination 625-632
68 schema:productId N1171b1d30a844ba18d0e77939b1c94e6
69 Nc429b086dddc483cbf23e7d2d37d32ea
70 Nc4a34154fa63417a8b66be94d0b9a37f
71 Nc7a8cd719d954664b92c5d2e839f718a
72 Nd104d1adfadb459d9b57ee4ec7725a22
73 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034809523
74 https://doi.org/10.1007/s00262-003-0496-2
75 schema:sdDatePublished 2019-04-10T15:05
76 schema:sdLicense https://scigraph.springernature.com/explorer/license/
77 schema:sdPublisher N6bf1568cde35489cb38b37c0aed92ce6
78 schema:url http://link.springer.com/10.1007%2Fs00262-003-0496-2
79 sgo:license sg:explorer/license/
80 sgo:sdDataset articles
81 rdf:type schema:ScholarlyArticle
82 N022aebcd18c14c01b8cff21a1d55e9ae schema:volumeNumber 53
83 rdf:type schema:PublicationVolume
84 N1171b1d30a844ba18d0e77939b1c94e6 schema:name readcube_id
85 schema:value a5d1b6aea866c78633610fe73211f9bf62fd33f005977c18fe92858d00d02ac7
86 rdf:type schema:PropertyValue
87 N1d5c5b56f3d94e3cbb2b7d9ab1e7a8fe schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
88 schema:name B-Lymphocytes
89 rdf:type schema:DefinedTerm
90 N284555ecab15439883cad5337f8bc2d0 rdf:first sg:person.01200355130.11
91 rdf:rest Nba5ffbf7a50e4ed38e056216bc858844
92 N31419f7d4ada44f5abb089f4ebc8b250 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name T-Lymphocytes
94 rdf:type schema:DefinedTerm
95 N4e9191cbd40148c082deb3fe2a27e299 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
96 schema:name Immunotherapy
97 rdf:type schema:DefinedTerm
98 N5a63a0e221284e36b6cd6b968613af02 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name CD3 Complex
100 rdf:type schema:DefinedTerm
101 N5c5dee8135504e1f8ae81c7eafa541d3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
102 schema:name Cytoplasmic Granules
103 rdf:type schema:DefinedTerm
104 N5d6ea036c986428baee5d0867e54f88a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
105 schema:name Antibodies, Bispecific
106 rdf:type schema:DefinedTerm
107 N63f5e3aa19fc48139bd746f9e5b7a325 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
108 schema:name CASP8 and FADD-Like Apoptosis Regulating Protein
109 rdf:type schema:DefinedTerm
110 N683bc8922b1942459d333341ba2f6b92 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
111 schema:name Carrier Proteins
112 rdf:type schema:DefinedTerm
113 N6b134474d36a42b980a7113c92e6d724 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
114 schema:name Apoptosis
115 rdf:type schema:DefinedTerm
116 N6bf1568cde35489cb38b37c0aed92ce6 schema:name Springer Nature - SN SciGraph project
117 rdf:type schema:Organization
118 N6ed17a1a215445b2ae05bcbb2e9092fe schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
119 schema:name Pore Forming Cytotoxic Proteins
120 rdf:type schema:DefinedTerm
121 N72f43b10f1134670ad15b7a9b7181e4f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
122 schema:name Lymphocyte Activation
123 rdf:type schema:DefinedTerm
124 N7cd30087efa4431aad4b6654db05ca2e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
125 schema:name Apoptosis Regulatory Proteins
126 rdf:type schema:DefinedTerm
127 N801d3fbb37134cd9a146e0666de280d3 rdf:first sg:person.01253507773.99
128 rdf:rest N284555ecab15439883cad5337f8bc2d0
129 N86e033eb50d94d089c39c30f4ffe6ae5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
130 schema:name Antigens, CD19
131 rdf:type schema:DefinedTerm
132 N9523a052b6a547cabc00941d14e2566e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
133 schema:name Ligands
134 rdf:type schema:DefinedTerm
135 N99a6c40d8cab418ebfe7727c5f846ef4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
136 schema:name Fas Ligand Protein
137 rdf:type schema:DefinedTerm
138 Na3b39b6486984a2a87bb23597ddaa7a9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
139 schema:name Tumor Necrosis Factor-alpha
140 rdf:type schema:DefinedTerm
141 Naa21ac8aacf546f486e1579e5d202c76 schema:issueNumber 7
142 rdf:type schema:PublicationIssue
143 Nae34fbfca9a74ea59c74201d94889139 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
144 schema:name Up-Regulation
145 rdf:type schema:DefinedTerm
146 Nb1f6a6764cb94fedb3cba2ed973bafd3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
147 schema:name Membrane Glycoproteins
148 rdf:type schema:DefinedTerm
149 Nba5ffbf7a50e4ed38e056216bc858844 rdf:first sg:person.01146675164.26
150 rdf:rest rdf:nil
151 Nbc46798a5dd64099a76ba81898be6cd1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
152 schema:name Humans
153 rdf:type schema:DefinedTerm
154 Nbfb02dcd26b1455f809e1578da2eaffd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
155 schema:name Cytotoxicity, Immunologic
156 rdf:type schema:DefinedTerm
157 Nc06133bdab5c42888d5676eebeddd286 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
158 schema:name Exocytosis
159 rdf:type schema:DefinedTerm
160 Nc429b086dddc483cbf23e7d2d37d32ea schema:name nlm_unique_id
161 schema:value 8605732
162 rdf:type schema:PropertyValue
163 Nc4a34154fa63417a8b66be94d0b9a37f schema:name dimensions_id
164 schema:value pub.1034809523
165 rdf:type schema:PropertyValue
166 Nc7a8cd719d954664b92c5d2e839f718a schema:name pubmed_id
167 schema:value 15175907
168 rdf:type schema:PropertyValue
169 Nd104d1adfadb459d9b57ee4ec7725a22 schema:name doi
170 schema:value 10.1007/s00262-003-0496-2
171 rdf:type schema:PropertyValue
172 Nd8f0131000e146828329aff7cfbffc0d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
173 schema:name T-Lymphocytes, Cytotoxic
174 rdf:type schema:DefinedTerm
175 Nea306575595546a392c9e73552550fea schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
176 schema:name Tumor Cells, Cultured
177 rdf:type schema:DefinedTerm
178 Neb141849b95a4a0696c7be6726264c52 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
179 schema:name Intracellular Signaling Peptides and Proteins
180 rdf:type schema:DefinedTerm
181 Nf0f143d0d8be4f12a097fe3a63e7e163 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
182 schema:name Perforin
183 rdf:type schema:DefinedTerm
184 Nf44da7989089448f825e4553252971a0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
185 schema:name TNF-Related Apoptosis-Inducing Ligand
186 rdf:type schema:DefinedTerm
187 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
188 schema:name Biological Sciences
189 rdf:type schema:DefinedTerm
190 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
191 schema:name Biochemistry and Cell Biology
192 rdf:type schema:DefinedTerm
193 sg:journal.1096240 schema:issn 0340-7004
194 1432-0851
195 schema:name Cancer Immunology, Immunotherapy
196 rdf:type schema:Periodical
197 sg:person.01146675164.26 schema:affiliation https://www.grid.ac/institutes/grid.491869.b
198 schema:familyName Bargou
199 schema:givenName Ralf C.
200 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01146675164.26
201 rdf:type schema:Person
202 sg:person.01200355130.11 schema:affiliation https://www.grid.ac/institutes/grid.419491.0
203 schema:familyName Bommert
204 schema:givenName Kurt
205 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01200355130.11
206 rdf:type schema:Person
207 sg:person.01253507773.99 schema:affiliation https://www.grid.ac/institutes/grid.9613.d
208 schema:familyName Gruen
209 schema:givenName Michael
210 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01253507773.99
211 rdf:type schema:Person
212 sg:pub.10.1007/s002620050357 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042485673
213 https://doi.org/10.1007/s002620050357
214 rdf:type schema:CreativeWork
215 sg:pub.10.1038/35037728 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022325441
216 https://doi.org/10.1038/35037728
217 rdf:type schema:CreativeWork
218 sg:pub.10.1038/sj.cr.7290053 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034520577
219 https://doi.org/10.1038/sj.cr.7290053
220 rdf:type schema:CreativeWork
221 sg:pub.10.1038/sj.leu.2402890 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000138204
222 https://doi.org/10.1038/sj.leu.2402890
223 rdf:type schema:CreativeWork
224 https://app.dimensions.ai/details/publication/pub.1074262614 schema:CreativeWork
225 https://app.dimensions.ai/details/publication/pub.1074361775 schema:CreativeWork
226 https://app.dimensions.ai/details/publication/pub.1074525887 schema:CreativeWork
227 https://app.dimensions.ai/details/publication/pub.1074601318 schema:CreativeWork
228 https://app.dimensions.ai/details/publication/pub.1074610551 schema:CreativeWork
229 https://app.dimensions.ai/details/publication/pub.1074889443 schema:CreativeWork
230 https://app.dimensions.ai/details/publication/pub.1074999528 schema:CreativeWork
231 https://app.dimensions.ai/details/publication/pub.1082882712 schema:CreativeWork
232 https://app.dimensions.ai/details/publication/pub.1083072553 schema:CreativeWork
233 https://doi.org/10.1002/(sici)1097-0215(19990301)80:5<715::aid-ijc15>3.0.co;2-p schema:sameAs https://app.dimensions.ai/details/publication/pub.1026331307
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1002/ijc.10557 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005681080
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1016/s0167-5699(00)01659-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001971615
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1016/s0171-2985(97)80062-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047053183
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1016/s0952-7915(00)00094-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031996667
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1074/jbc.271.46.29073 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034054657
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1084/jem.175.2.553 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005248559
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1093/jnci/85.12.1003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059817975
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1146/annurev.immunol.20.100201.131730 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052629241
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1182/blood.v98.5.1326 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040463948
252 rdf:type schema:CreativeWork
253 https://doi.org/10.3322/canjclin.49.1.8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044953296
254 rdf:type schema:CreativeWork
255 https://doi.org/10.4049/jimmunol.165.6.3023 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074694833
256 rdf:type schema:CreativeWork
257 https://doi.org/10.4049/jimmunol.165.8.4405 schema:sameAs https://app.dimensions.ai/details/publication/pub.1074713293
258 rdf:type schema:CreativeWork
259 https://doi.org/10.4049/jimmunol.170.8.4397 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049032729
260 rdf:type schema:CreativeWork
261 https://www.grid.ac/institutes/grid.419491.0 schema:alternateName Max Delbrück Center for Molecular Medicine
262 schema:name Max Delbrück Center for Molecular Medicine, Robert-Rössle-Str. 10, 13125, Berlin, Germany
263 rdf:type schema:Organization
264 https://www.grid.ac/institutes/grid.491869.b schema:alternateName Helios Hospital Berlin-Buch
265 schema:name Department of Hematology, Oncology and Tumorimmunology, Robert Rössle Clinic, Charité, Humboldt University of Berlin, Berlin, Germany
266 Helios Klinikum Berlin-Buch, Berlin, Germany
267 Max Delbrück Center for Molecular Medicine, Robert-Rössle-Str. 10, 13125, Berlin, Germany
268 rdf:type schema:Organization
269 https://www.grid.ac/institutes/grid.9613.d schema:alternateName Friedrich Schiller University Jena
270 schema:name Department of Hematology, Oncology and Tumorimmunology, Robert Rössle Clinic, Charité, Humboldt University of Berlin, Berlin, Germany
271 Max Delbrück Center for Molecular Medicine, Robert-Rössle-Str. 10, 13125, Berlin, Germany
272 Research Unit Molecular Cell Biology, University of Jena, Jena, Germany
273 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...