Feasibility of sodium/iodide symporter gene as a new imaging reporter gene: comparison with HSV1-tk View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2003-12-19

AUTHORS

Jae Hoon Shin, June-Key Chung, Joo Hyun Kang, Yong Jin Lee, Kwang Il Kim, Chul Woo Kim, Jae Min Jeong, Dong Soo Lee, Myung Chul Lee

ABSTRACT

Positron emission tomography (PET) imaging reporter genes, such as HSV1-tk and D2 receptor genes, make it possible to visualise gene expression non-invasively and repetitively in vivo. However, these systems require the synthesis of complicated substrates and the availability of expensive PET equipment. Expression of the sodium/iodide symporter (NIS) gene can be easily monitored with radioiodines and technetium-99m using a gamma camera. To evaluate the possibility of using NIS as an imaging reporter gene, we compared its characteristics with those of the conventional HSV1-tk gene. The CM cell line was made by transfecting the HSV1-tk gene into CT-26 (mouse colon carcinoma cell line). The CTN and CMN cell lines were then made by transfecting the NIS gene into CT-26 and CM. We measured the uptake of iodine-125 iodovinyldeoxyuridine ([125I]IVDU) and 125I to evaluate the expression of the HSV1-tk and NIS genes, respectively. Each cell line was injected into four flank sites in Balb/c mice. The biodistribution study was performed after intravenously injecting [125I]IVDU and 131I, and 131I scintigraphy was performed for the evaluation of NIS expression. In vitro studies indicated that CTN and CMN had 40- to 79-fold and 150- to 256-fold higher uptake of 125I than CT-26 and CM, respectively. Furthermore, CM and CMN showed 57- to 69-fold higher uptake of [125I]IVDU than CT-26 and CTN. NIS gene expression and 125I accumulation were found to be directly correlated (R2=0.923), as were HSV1-tk gene expression and [125I]IVDU accumulation (R2=0.956). Calculated signal per unit NIS and HSV1-tk mRNA expression was 23,240±3,755 cpm and 34,039±5,346 cpm, respectively. In vivo study indicated that CTN and CMN had 2.3- and 5.8-fold higher uptake of 131I than CT-26 and CM, and 1.8- and 3.5-fold higher uptake of [125I]IVDU than CT-26 and CTN. Scintigraphy using 131I easily visualised CTN and CMN tumours. In conclusion, the NIS gene may be viewed as an imaging reporter gene with comparable performance to the HSV1-tk gene for monitoring target gene expression. More... »

PAGES

425-432

References to SciGraph publications

  • 2001-07-01. Direct correlation between positron emission tomographic images of two reporter genes delivered by two distinct adenoviral vectors in GENE THERAPY
  • 2001-12. Annexin-V imaging for noninvasive detection of cardiac allograft rejection in NATURE MEDICINE
  • 2002-04-11. Establishment of radioactive astatine and iodine uptake in cancer cell lines expressing the human sodium/iodide symporter in EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
  • 2001-06. In vivo molecular target assessment of matrix metalloproteinase inhibition in NATURE MEDICINE
  • 2002-01. Functional genomics and proteomics – the role of nuclear medicine in EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
  • 2000-08. Quantification of target gene expression by imaging reporter gene expression in living animals in NATURE MEDICINE
  • 2002-09. Molecular imaging of cancer with positron emission tomography in NATURE REVIEWS CANCER
  • 1999-05-01. Repetitive, non-invasive imaging of the dopamine D2 receptor as a reporter gene in living animals in GENE THERAPY
  • 2001-10-01. In vivo sodium iodide symporter gene therapy of prostate cancer in GENE THERAPY
  • 1998-06-01. Imaging and tissue biodistribution of 99mTc-labeled adenovirus knob (serotype 5) in GENE THERAPY
  • 2002-08-13. In vivo imaging and radioiodine therapy following sodium iodide symporter gene transfer in animal model of intracerebral gliomas in GENE THERAPY
  • 2002-01. Molecular imaging of small animals with dedicated PET tomographs in EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
  • 1996-02. Cloning and characterization of the thyroid iodide transporter in NATURE
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00259-003-1394-8

    DOI

    http://dx.doi.org/10.1007/s00259-003-1394-8

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1051529397

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/14689242


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Medical and Health Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1103", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Clinical Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Animals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Biomarkers, Tumor", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cell Line, Tumor", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Colonic Neoplasms", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Feasibility Studies", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Expression Profiling", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genes, Reporter", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Herpesvirus 1, Human", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Idoxuridine", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mice", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Organ Specificity", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Radionuclide Imaging", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Radiopharmaceuticals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Symporters", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Thymidine Kinase", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Tissue Distribution", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Transfection", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Laboratory of Molecular Imaging and Therapy, Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea", 
              "id": "http://www.grid.ac/institutes/grid.31501.36", 
              "name": [
                "Department of Nuclear Medicine, Cancer Research Institute, Seoul National University College of Medicine, 28 Yongon-dong, 110-744, Chongno-gu, Seoul, Korea", 
                "Department of Tumor Biology, Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea", 
                "Laboratory of Molecular Imaging and Therapy, Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Shin", 
            "givenName": "Jae Hoon", 
            "id": "sg:person.0576155617.47", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0576155617.47"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Laboratory of Molecular Imaging and Therapy, Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea", 
              "id": "http://www.grid.ac/institutes/grid.31501.36", 
              "name": [
                "Department of Nuclear Medicine, Cancer Research Institute, Seoul National University College of Medicine, 28 Yongon-dong, 110-744, Chongno-gu, Seoul, Korea", 
                "Department of Tumor Biology, Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea", 
                "Laboratory of Molecular Imaging and Therapy, Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Chung", 
            "givenName": "June-Key", 
            "id": "sg:person.0751347234.39", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0751347234.39"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Laboratory of Molecular Imaging and Therapy, Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea", 
              "id": "http://www.grid.ac/institutes/grid.31501.36", 
              "name": [
                "Department of Nuclear Medicine, Cancer Research Institute, Seoul National University College of Medicine, 28 Yongon-dong, 110-744, Chongno-gu, Seoul, Korea", 
                "Laboratory of Molecular Imaging and Therapy, Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kang", 
            "givenName": "Joo Hyun", 
            "id": "sg:person.01356373637.49", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01356373637.49"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Laboratory of Molecular Imaging and Therapy, Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea", 
              "id": "http://www.grid.ac/institutes/grid.31501.36", 
              "name": [
                "Department of Nuclear Medicine, Cancer Research Institute, Seoul National University College of Medicine, 28 Yongon-dong, 110-744, Chongno-gu, Seoul, Korea", 
                "Department of Tumor Biology, Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea", 
                "Laboratory of Molecular Imaging and Therapy, Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Lee", 
            "givenName": "Yong Jin", 
            "id": "sg:person.0724572466.08", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0724572466.08"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Laboratory of Molecular Imaging and Therapy, Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea", 
              "id": "http://www.grid.ac/institutes/grid.31501.36", 
              "name": [
                "Department of Nuclear Medicine, Cancer Research Institute, Seoul National University College of Medicine, 28 Yongon-dong, 110-744, Chongno-gu, Seoul, Korea", 
                "Department of Tumor Biology, Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea", 
                "Laboratory of Molecular Imaging and Therapy, Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kim", 
            "givenName": "Kwang Il", 
            "id": "sg:person.01167722776.13", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01167722776.13"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Pathology, Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea", 
              "id": "http://www.grid.ac/institutes/grid.31501.36", 
              "name": [
                "Department of Pathology, Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kim", 
            "givenName": "Chul Woo", 
            "id": "sg:person.01152623257.56", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01152623257.56"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Laboratory of Molecular Imaging and Therapy, Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea", 
              "id": "http://www.grid.ac/institutes/grid.31501.36", 
              "name": [
                "Department of Nuclear Medicine, Cancer Research Institute, Seoul National University College of Medicine, 28 Yongon-dong, 110-744, Chongno-gu, Seoul, Korea", 
                "Laboratory of Molecular Imaging and Therapy, Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Jeong", 
            "givenName": "Jae Min", 
            "id": "sg:person.01301360400.94", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01301360400.94"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Laboratory of Molecular Imaging and Therapy, Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea", 
              "id": "http://www.grid.ac/institutes/grid.31501.36", 
              "name": [
                "Department of Nuclear Medicine, Cancer Research Institute, Seoul National University College of Medicine, 28 Yongon-dong, 110-744, Chongno-gu, Seoul, Korea", 
                "Laboratory of Molecular Imaging and Therapy, Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Lee", 
            "givenName": "Dong Soo", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Nuclear Medicine, Cancer Research Institute, Seoul National University College of Medicine, 28 Yongon-dong, 110-744, Chongno-gu, Seoul, Korea", 
              "id": "http://www.grid.ac/institutes/grid.31501.36", 
              "name": [
                "Department of Nuclear Medicine, Cancer Research Institute, Seoul National University College of Medicine, 28 Yongon-dong, 110-744, Chongno-gu, Seoul, Korea"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Lee", 
            "givenName": "Myung Chul", 
            "id": "sg:person.01337220140.25", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01337220140.25"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1038/sj.gt.3300659", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002153931", 
              "https://doi.org/10.1038/sj.gt.3300659"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/sj.gt.3301490", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004860596", 
              "https://doi.org/10.1038/sj.gt.3301490"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/78704", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036861569", 
              "https://doi.org/10.1038/78704"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/sj.gt.3301787", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022895142", 
              "https://doi.org/10.1038/sj.gt.3301787"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00259-002-0784-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014654388", 
              "https://doi.org/10.1007/s00259-002-0784-7"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00259-001-0683-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018614151", 
              "https://doi.org/10.1007/s00259-001-0683-3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/sj.gt.3300877", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014043920", 
              "https://doi.org/10.1038/sj.gt.3300877"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/sj.gt.3301558", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1007301948", 
              "https://doi.org/10.1038/sj.gt.3301558"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrc882", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036705264", 
              "https://doi.org/10.1038/nrc882"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nm1201-1347", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011016385", 
              "https://doi.org/10.1038/nm1201-1347"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/379458a0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016268461", 
              "https://doi.org/10.1038/379458a0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/89126", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038138866", 
              "https://doi.org/10.1038/89126"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00259-001-0682-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031716696", 
              "https://doi.org/10.1007/s00259-001-0682-4"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2003-12-19", 
        "datePublishedReg": "2003-12-19", 
        "description": "Positron emission tomography (PET) imaging reporter genes, such as HSV1-tk and D2 receptor genes, make it possible to visualise gene expression non-invasively and repetitively in vivo. However, these systems require the synthesis of complicated substrates and the availability of expensive PET equipment. Expression of the sodium/iodide symporter (NIS) gene can be easily monitored with radioiodines and technetium-99m using a gamma camera. To evaluate the possibility of using NIS as an imaging reporter gene, we compared its characteristics with those of the conventional HSV1-tk gene. The CM cell line was made by transfecting the HSV1-tk gene into CT-26 (mouse colon carcinoma cell line). The CTN and CMN cell lines were then made by transfecting the NIS gene into CT-26 and CM. We measured the uptake of iodine-125 iodovinyldeoxyuridine ([125I]IVDU) and 125I to evaluate the expression of the HSV1-tk and NIS genes, respectively. Each cell line was injected into four flank sites in Balb/c mice. The biodistribution study was performed after intravenously injecting [125I]IVDU and 131I, and 131I scintigraphy was performed for the evaluation of NIS expression. In vitro studies indicated that CTN and CMN had 40- to 79-fold and 150- to 256-fold higher uptake of 125I than CT-26 and CM, respectively. Furthermore, CM and CMN showed 57- to 69-fold higher uptake of [125I]IVDU than CT-26 and CTN. NIS gene expression and 125I accumulation were found to be directly correlated (R2=0.923), as were HSV1-tk gene expression and [125I]IVDU accumulation (R2=0.956). Calculated signal per unit NIS and HSV1-tk mRNA expression was 23,240\u00b13,755\u00a0cpm and 34,039\u00b15,346\u00a0cpm, respectively. In vivo study indicated that CTN and CMN had 2.3- and 5.8-fold higher uptake of 131I than CT-26 and CM, and 1.8- and 3.5-fold higher uptake of [125I]IVDU than CT-26 and CTN. Scintigraphy using 131I easily visualised CTN and CMN tumours. In conclusion, the NIS gene may be viewed as an imaging reporter gene with comparable performance to the HSV1-tk gene for monitoring target gene expression.", 
        "genre": "article", 
        "id": "sg:pub.10.1007/s00259-003-1394-8", 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1297401", 
            "issn": [
              "1619-7070", 
              "1619-7089"
            ], 
            "name": "European Journal of Nuclear Medicine and Molecular Imaging", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "3", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "31"
          }
        ], 
        "keywords": [
          "imaging reporter gene", 
          "HSV1-tk gene", 
          "sodium/iodide symporter gene", 
          "NIS gene", 
          "CT-26", 
          "high uptake", 
          "BALB/c mice", 
          "cell lines", 
          "HSV1-tk", 
          "symporter gene", 
          "HSV1-tk gene expression", 
          "CM cell lines", 
          "gene expression", 
          "NIS gene expression", 
          "D2 receptor gene", 
          "c mice", 
          "NIS expression", 
          "mRNA expression", 
          "biodistribution studies", 
          "reporter gene", 
          "vivo studies", 
          "receptor gene", 
          "cTn", 
          "scintigraphy", 
          "PET equipment", 
          "target gene expression", 
          "gamma camera", 
          "expression", 
          "uptake", 
          "tumors", 
          "genes", 
          "NIS", 
          "mice", 
          "radioiodine", 
          "study", 
          "technetium", 
          "CPM", 
          "flank sites", 
          "vivo", 
          "CM", 
          "accumulation", 
          "conclusion", 
          "CMN", 
          "lines", 
          "evaluation", 
          "sites", 
          "feasibility", 
          "comparison", 
          "availability", 
          "characteristics", 
          "possibility", 
          "synthesis", 
          "system", 
          "signals", 
          "equipment", 
          "substrate", 
          "comparable performance", 
          "complicated substrates", 
          "performance", 
          "camera"
        ], 
        "name": "Feasibility of sodium/iodide symporter gene as a new imaging reporter gene: comparison with HSV1-tk", 
        "pagination": "425-432", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1051529397"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/s00259-003-1394-8"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "14689242"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/s00259-003-1394-8", 
          "https://app.dimensions.ai/details/publication/pub.1051529397"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2022-10-01T06:32", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20221001/entities/gbq_results/article/article_376.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1007/s00259-003-1394-8"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00259-003-1394-8'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00259-003-1394-8'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00259-003-1394-8'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00259-003-1394-8'


     

    This table displays all metadata directly associated to this object as RDF triples.

    305 TRIPLES      21 PREDICATES      116 URIs      95 LITERALS      25 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/s00259-003-1394-8 schema:about N020346b47f9a40da9b304ef816a28ffc
    2 N0dc5684406224bcbbcc9b60ca3c361d7
    3 N144a9fd7f53947a48941e36db2ae382f
    4 N14fca58849bd4c019c3cedc6b562e59f
    5 N48c3fbb04bad46c58d57884487b9e202
    6 N6b9c566459064cb4a126ec029c8683df
    7 N716817ff4b9f4878a36a55c52dd5f1d4
    8 N778cec30d6364015a1624be32cef75b6
    9 N927716b2c3204f8cbed8a6ba0f3b9d42
    10 N9455a268b0884d6181601e568a69cd04
    11 N95ca49ae764f4e76b4f1e04f9990be22
    12 Nb11248111bd24e62988e496265d07112
    13 Nbce1d10587294c23be6794a4a6ca0b4c
    14 Nbdb1f87016ac4c4eadbd427838d15436
    15 Ncdb0d5c0bccc406a98c81195facc09cd
    16 Nce110408a8de4a41b98278002a0236f8
    17 Ndecceb21691645dca154e5da1e94388a
    18 Nf61223a538d64f7ebf4c426495b450f3
    19 anzsrc-for:11
    20 anzsrc-for:1103
    21 schema:author N262b81f8d78f4056b46131f5bf9583c5
    22 schema:citation sg:pub.10.1007/s00259-001-0682-4
    23 sg:pub.10.1007/s00259-001-0683-3
    24 sg:pub.10.1007/s00259-002-0784-7
    25 sg:pub.10.1038/379458a0
    26 sg:pub.10.1038/78704
    27 sg:pub.10.1038/89126
    28 sg:pub.10.1038/nm1201-1347
    29 sg:pub.10.1038/nrc882
    30 sg:pub.10.1038/sj.gt.3300659
    31 sg:pub.10.1038/sj.gt.3300877
    32 sg:pub.10.1038/sj.gt.3301490
    33 sg:pub.10.1038/sj.gt.3301558
    34 sg:pub.10.1038/sj.gt.3301787
    35 schema:datePublished 2003-12-19
    36 schema:datePublishedReg 2003-12-19
    37 schema:description Positron emission tomography (PET) imaging reporter genes, such as HSV1-tk and D2 receptor genes, make it possible to visualise gene expression non-invasively and repetitively in vivo. However, these systems require the synthesis of complicated substrates and the availability of expensive PET equipment. Expression of the sodium/iodide symporter (NIS) gene can be easily monitored with radioiodines and technetium-99m using a gamma camera. To evaluate the possibility of using NIS as an imaging reporter gene, we compared its characteristics with those of the conventional HSV1-tk gene. The CM cell line was made by transfecting the HSV1-tk gene into CT-26 (mouse colon carcinoma cell line). The CTN and CMN cell lines were then made by transfecting the NIS gene into CT-26 and CM. We measured the uptake of iodine-125 iodovinyldeoxyuridine ([125I]IVDU) and 125I to evaluate the expression of the HSV1-tk and NIS genes, respectively. Each cell line was injected into four flank sites in Balb/c mice. The biodistribution study was performed after intravenously injecting [125I]IVDU and 131I, and 131I scintigraphy was performed for the evaluation of NIS expression. In vitro studies indicated that CTN and CMN had 40- to 79-fold and 150- to 256-fold higher uptake of 125I than CT-26 and CM, respectively. Furthermore, CM and CMN showed 57- to 69-fold higher uptake of [125I]IVDU than CT-26 and CTN. NIS gene expression and 125I accumulation were found to be directly correlated (R2=0.923), as were HSV1-tk gene expression and [125I]IVDU accumulation (R2=0.956). Calculated signal per unit NIS and HSV1-tk mRNA expression was 23,240±3,755 cpm and 34,039±5,346 cpm, respectively. In vivo study indicated that CTN and CMN had 2.3- and 5.8-fold higher uptake of 131I than CT-26 and CM, and 1.8- and 3.5-fold higher uptake of [125I]IVDU than CT-26 and CTN. Scintigraphy using 131I easily visualised CTN and CMN tumours. In conclusion, the NIS gene may be viewed as an imaging reporter gene with comparable performance to the HSV1-tk gene for monitoring target gene expression.
    38 schema:genre article
    39 schema:isAccessibleForFree false
    40 schema:isPartOf N6d9bbf2bd1ae47d6800d02b4f4411bd3
    41 N80a383beacb946c8bc66a5443eaf7bf0
    42 sg:journal.1297401
    43 schema:keywords BALB/c mice
    44 CM
    45 CM cell lines
    46 CMN
    47 CPM
    48 CT-26
    49 D2 receptor gene
    50 HSV1-tk
    51 HSV1-tk gene
    52 HSV1-tk gene expression
    53 NIS
    54 NIS expression
    55 NIS gene
    56 NIS gene expression
    57 PET equipment
    58 accumulation
    59 availability
    60 biodistribution studies
    61 c mice
    62 cTn
    63 camera
    64 cell lines
    65 characteristics
    66 comparable performance
    67 comparison
    68 complicated substrates
    69 conclusion
    70 equipment
    71 evaluation
    72 expression
    73 feasibility
    74 flank sites
    75 gamma camera
    76 gene expression
    77 genes
    78 high uptake
    79 imaging reporter gene
    80 lines
    81 mRNA expression
    82 mice
    83 performance
    84 possibility
    85 radioiodine
    86 receptor gene
    87 reporter gene
    88 scintigraphy
    89 signals
    90 sites
    91 sodium/iodide symporter gene
    92 study
    93 substrate
    94 symporter gene
    95 synthesis
    96 system
    97 target gene expression
    98 technetium
    99 tumors
    100 uptake
    101 vivo
    102 vivo studies
    103 schema:name Feasibility of sodium/iodide symporter gene as a new imaging reporter gene: comparison with HSV1-tk
    104 schema:pagination 425-432
    105 schema:productId N9e2e81274acc46ca9bef44e3bc98b573
    106 Na672e9c33abc4297bf442bcddffb6ac6
    107 Nf48449cf51ba4a148b00a6e30161efe5
    108 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051529397
    109 https://doi.org/10.1007/s00259-003-1394-8
    110 schema:sdDatePublished 2022-10-01T06:32
    111 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    112 schema:sdPublisher N952d82689f66407ba384f97856db02bc
    113 schema:url https://doi.org/10.1007/s00259-003-1394-8
    114 sgo:license sg:explorer/license/
    115 sgo:sdDataset articles
    116 rdf:type schema:ScholarlyArticle
    117 N011c259edc9740f09d0014d8b2dd8d40 rdf:first sg:person.01301360400.94
    118 rdf:rest Nddc4281002774d70b8cc6713eb441481
    119 N020346b47f9a40da9b304ef816a28ffc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    120 schema:name Cell Line, Tumor
    121 rdf:type schema:DefinedTerm
    122 N0dc5684406224bcbbcc9b60ca3c361d7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    123 schema:name Animals
    124 rdf:type schema:DefinedTerm
    125 N144a9fd7f53947a48941e36db2ae382f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    126 schema:name Idoxuridine
    127 rdf:type schema:DefinedTerm
    128 N14fca58849bd4c019c3cedc6b562e59f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    129 schema:name Transfection
    130 rdf:type schema:DefinedTerm
    131 N25760d0f87564891b6b58c790a5e9361 rdf:first sg:person.01337220140.25
    132 rdf:rest rdf:nil
    133 N262b81f8d78f4056b46131f5bf9583c5 rdf:first sg:person.0576155617.47
    134 rdf:rest N84410a870ad84bfc9ce6725d10fd3e9c
    135 N48c3fbb04bad46c58d57884487b9e202 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    136 schema:name Biomarkers, Tumor
    137 rdf:type schema:DefinedTerm
    138 N6b9c566459064cb4a126ec029c8683df schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    139 schema:name Radiopharmaceuticals
    140 rdf:type schema:DefinedTerm
    141 N6d9bbf2bd1ae47d6800d02b4f4411bd3 schema:volumeNumber 31
    142 rdf:type schema:PublicationVolume
    143 N716817ff4b9f4878a36a55c52dd5f1d4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    144 schema:name Feasibility Studies
    145 rdf:type schema:DefinedTerm
    146 N735513e945884e1b8c8bb67ec90243c5 rdf:first sg:person.01152623257.56
    147 rdf:rest N011c259edc9740f09d0014d8b2dd8d40
    148 N778cec30d6364015a1624be32cef75b6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    149 schema:name Colonic Neoplasms
    150 rdf:type schema:DefinedTerm
    151 N7926e7a3465d4d6dbad1174236562fe6 rdf:first sg:person.01167722776.13
    152 rdf:rest N735513e945884e1b8c8bb67ec90243c5
    153 N80a383beacb946c8bc66a5443eaf7bf0 schema:issueNumber 3
    154 rdf:type schema:PublicationIssue
    155 N84410a870ad84bfc9ce6725d10fd3e9c rdf:first sg:person.0751347234.39
    156 rdf:rest Ne720217bb1974d6a8ddcbe5c61110695
    157 N90eb2d1d973542e0aef64cfaac05f61d rdf:first sg:person.0724572466.08
    158 rdf:rest N7926e7a3465d4d6dbad1174236562fe6
    159 N927716b2c3204f8cbed8a6ba0f3b9d42 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    160 schema:name Radionuclide Imaging
    161 rdf:type schema:DefinedTerm
    162 N9455a268b0884d6181601e568a69cd04 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    163 schema:name Tissue Distribution
    164 rdf:type schema:DefinedTerm
    165 N952d82689f66407ba384f97856db02bc schema:name Springer Nature - SN SciGraph project
    166 rdf:type schema:Organization
    167 N95ca49ae764f4e76b4f1e04f9990be22 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    168 schema:name Symporters
    169 rdf:type schema:DefinedTerm
    170 N9e2e81274acc46ca9bef44e3bc98b573 schema:name doi
    171 schema:value 10.1007/s00259-003-1394-8
    172 rdf:type schema:PropertyValue
    173 Na672e9c33abc4297bf442bcddffb6ac6 schema:name pubmed_id
    174 schema:value 14689242
    175 rdf:type schema:PropertyValue
    176 Nb11248111bd24e62988e496265d07112 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    177 schema:name Mice
    178 rdf:type schema:DefinedTerm
    179 Nbce1d10587294c23be6794a4a6ca0b4c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    180 schema:name Organ Specificity
    181 rdf:type schema:DefinedTerm
    182 Nbdb1f87016ac4c4eadbd427838d15436 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    183 schema:name Humans
    184 rdf:type schema:DefinedTerm
    185 Nc0ff8b1108e14f8a9aa33f55385858be schema:affiliation grid-institutes:grid.31501.36
    186 schema:familyName Lee
    187 schema:givenName Dong Soo
    188 rdf:type schema:Person
    189 Ncdb0d5c0bccc406a98c81195facc09cd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    190 schema:name Gene Expression Profiling
    191 rdf:type schema:DefinedTerm
    192 Nce110408a8de4a41b98278002a0236f8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    193 schema:name Genes, Reporter
    194 rdf:type schema:DefinedTerm
    195 Nddc4281002774d70b8cc6713eb441481 rdf:first Nc0ff8b1108e14f8a9aa33f55385858be
    196 rdf:rest N25760d0f87564891b6b58c790a5e9361
    197 Ndecceb21691645dca154e5da1e94388a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    198 schema:name Herpesvirus 1, Human
    199 rdf:type schema:DefinedTerm
    200 Ne720217bb1974d6a8ddcbe5c61110695 rdf:first sg:person.01356373637.49
    201 rdf:rest N90eb2d1d973542e0aef64cfaac05f61d
    202 Nf48449cf51ba4a148b00a6e30161efe5 schema:name dimensions_id
    203 schema:value pub.1051529397
    204 rdf:type schema:PropertyValue
    205 Nf61223a538d64f7ebf4c426495b450f3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    206 schema:name Thymidine Kinase
    207 rdf:type schema:DefinedTerm
    208 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
    209 schema:name Medical and Health Sciences
    210 rdf:type schema:DefinedTerm
    211 anzsrc-for:1103 schema:inDefinedTermSet anzsrc-for:
    212 schema:name Clinical Sciences
    213 rdf:type schema:DefinedTerm
    214 sg:journal.1297401 schema:issn 1619-7070
    215 1619-7089
    216 schema:name European Journal of Nuclear Medicine and Molecular Imaging
    217 schema:publisher Springer Nature
    218 rdf:type schema:Periodical
    219 sg:person.01152623257.56 schema:affiliation grid-institutes:grid.31501.36
    220 schema:familyName Kim
    221 schema:givenName Chul Woo
    222 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01152623257.56
    223 rdf:type schema:Person
    224 sg:person.01167722776.13 schema:affiliation grid-institutes:grid.31501.36
    225 schema:familyName Kim
    226 schema:givenName Kwang Il
    227 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01167722776.13
    228 rdf:type schema:Person
    229 sg:person.01301360400.94 schema:affiliation grid-institutes:grid.31501.36
    230 schema:familyName Jeong
    231 schema:givenName Jae Min
    232 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01301360400.94
    233 rdf:type schema:Person
    234 sg:person.01337220140.25 schema:affiliation grid-institutes:grid.31501.36
    235 schema:familyName Lee
    236 schema:givenName Myung Chul
    237 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01337220140.25
    238 rdf:type schema:Person
    239 sg:person.01356373637.49 schema:affiliation grid-institutes:grid.31501.36
    240 schema:familyName Kang
    241 schema:givenName Joo Hyun
    242 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01356373637.49
    243 rdf:type schema:Person
    244 sg:person.0576155617.47 schema:affiliation grid-institutes:grid.31501.36
    245 schema:familyName Shin
    246 schema:givenName Jae Hoon
    247 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0576155617.47
    248 rdf:type schema:Person
    249 sg:person.0724572466.08 schema:affiliation grid-institutes:grid.31501.36
    250 schema:familyName Lee
    251 schema:givenName Yong Jin
    252 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0724572466.08
    253 rdf:type schema:Person
    254 sg:person.0751347234.39 schema:affiliation grid-institutes:grid.31501.36
    255 schema:familyName Chung
    256 schema:givenName June-Key
    257 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0751347234.39
    258 rdf:type schema:Person
    259 sg:pub.10.1007/s00259-001-0682-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031716696
    260 https://doi.org/10.1007/s00259-001-0682-4
    261 rdf:type schema:CreativeWork
    262 sg:pub.10.1007/s00259-001-0683-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018614151
    263 https://doi.org/10.1007/s00259-001-0683-3
    264 rdf:type schema:CreativeWork
    265 sg:pub.10.1007/s00259-002-0784-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014654388
    266 https://doi.org/10.1007/s00259-002-0784-7
    267 rdf:type schema:CreativeWork
    268 sg:pub.10.1038/379458a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016268461
    269 https://doi.org/10.1038/379458a0
    270 rdf:type schema:CreativeWork
    271 sg:pub.10.1038/78704 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036861569
    272 https://doi.org/10.1038/78704
    273 rdf:type schema:CreativeWork
    274 sg:pub.10.1038/89126 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038138866
    275 https://doi.org/10.1038/89126
    276 rdf:type schema:CreativeWork
    277 sg:pub.10.1038/nm1201-1347 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011016385
    278 https://doi.org/10.1038/nm1201-1347
    279 rdf:type schema:CreativeWork
    280 sg:pub.10.1038/nrc882 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036705264
    281 https://doi.org/10.1038/nrc882
    282 rdf:type schema:CreativeWork
    283 sg:pub.10.1038/sj.gt.3300659 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002153931
    284 https://doi.org/10.1038/sj.gt.3300659
    285 rdf:type schema:CreativeWork
    286 sg:pub.10.1038/sj.gt.3300877 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014043920
    287 https://doi.org/10.1038/sj.gt.3300877
    288 rdf:type schema:CreativeWork
    289 sg:pub.10.1038/sj.gt.3301490 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004860596
    290 https://doi.org/10.1038/sj.gt.3301490
    291 rdf:type schema:CreativeWork
    292 sg:pub.10.1038/sj.gt.3301558 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007301948
    293 https://doi.org/10.1038/sj.gt.3301558
    294 rdf:type schema:CreativeWork
    295 sg:pub.10.1038/sj.gt.3301787 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022895142
    296 https://doi.org/10.1038/sj.gt.3301787
    297 rdf:type schema:CreativeWork
    298 grid-institutes:grid.31501.36 schema:alternateName Department of Nuclear Medicine, Cancer Research Institute, Seoul National University College of Medicine, 28 Yongon-dong, 110-744, Chongno-gu, Seoul, Korea
    299 Department of Pathology, Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea
    300 Laboratory of Molecular Imaging and Therapy, Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea
    301 schema:name Department of Nuclear Medicine, Cancer Research Institute, Seoul National University College of Medicine, 28 Yongon-dong, 110-744, Chongno-gu, Seoul, Korea
    302 Department of Pathology, Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea
    303 Department of Tumor Biology, Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea
    304 Laboratory of Molecular Imaging and Therapy, Cancer Research Institute, Seoul National University College of Medicine, Seoul, Korea
    305 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...