Use of catabolite repression mutants for fermentation of sugar mixtures to ethanol View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2001-07

AUTHORS

N. Nichols, B. Dien, R. Bothast

ABSTRACT

. Use of agricultural biomass, other than cornstarch, to produce fuel ethanol requires a microorganism that can ferment the mixture of sugars derived from hemicellulose. Escherichiacoli metabolizes a wide range of substrates and has been engineered to produce ethanol in high yield from sugar mixtures. E. coli metabolizes glucose in preference to other sugars and, as a result, utilization of the pentoses in hemicellulose-derived sugar mixtures is delayed and may be incomplete. Residual sugar lowers the ethanol yield and is problematic for downstream processing of fermentation products. Therefore, a catabolite repression mutant that simultaneously utilizes glucose and pentoses would be useful for fermentation of complex substrate mixtures. We constructed ethanologenic E. coli strains with a glucose phosphotransferase (ptsG) mutation and used the mutants to ferment glucose, arabinose, and xylose, singly and in mixtures, to ethanol. Yields were 87–94% of theoretical for both the wild type and mutants, but the mutants had an altered pattern of mixed sugar utilization. Phosphotransferase mutants metabolized the pentoses simultaneously with glucose, rather than sequentially. Based upon fermentations of sugar mixtures, a catabolite-repression mutant of ethanologenic E. coli is expected to provide more efficient fermentation of hemicellulose hydrolysates by allowing direct utilization of pentoses. More... »

PAGES

120-125

Journal

TITLE

Applied Microbiology and Biotechnology

ISSUE

1

VOLUME

56

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s002530100628

DOI

http://dx.doi.org/10.1007/s002530100628

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1031253667

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/11499918


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0605", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Microbiology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Biomass", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Carbohydrate Metabolism", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Escherichia coli", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Ethanol", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fermentation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Phosphoenolpyruvate Sugar Phosphotransferase System", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Fermentation Biochemistry Research Unit, National Center for Agricultural Utilization Research, USDA, Agricultural Research Service, 1815\u00a0N. University Street, Peoria, IL\u00a061604, USA", 
          "id": "http://www.grid.ac/institutes/grid.507311.1", 
          "name": [
            "Fermentation Biochemistry Research Unit, National Center for Agricultural Utilization Research, USDA, Agricultural Research Service, 1815\u00a0N. University Street, Peoria, IL\u00a061604, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Nichols", 
        "givenName": "N.", 
        "id": "sg:person.01362477120.95", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01362477120.95"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Fermentation Biochemistry Research Unit, National Center for Agricultural Utilization Research, USDA, Agricultural Research Service, 1815\u00a0N. University Street, Peoria, IL\u00a061604, USA", 
          "id": "http://www.grid.ac/institutes/grid.507311.1", 
          "name": [
            "Fermentation Biochemistry Research Unit, National Center for Agricultural Utilization Research, USDA, Agricultural Research Service, 1815\u00a0N. University Street, Peoria, IL\u00a061604, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Dien", 
        "givenName": "B.", 
        "id": "sg:person.0643421433.32", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0643421433.32"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Fermentation Biochemistry Research Unit, National Center for Agricultural Utilization Research, USDA, Agricultural Research Service, 1815\u00a0N. University Street, Peoria, IL\u00a061604, USA", 
          "id": "http://www.grid.ac/institutes/grid.507311.1", 
          "name": [
            "Fermentation Biochemistry Research Unit, National Center for Agricultural Utilization Research, USDA, Agricultural Research Service, 1815\u00a0N. University Street, Peoria, IL\u00a061604, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bothast", 
        "givenName": "R.", 
        "id": "sg:person.077623511.44", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.077623511.44"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "2001-07", 
    "datePublishedReg": "2001-07-01", 
    "description": "Abstract. Use of agricultural biomass, other than cornstarch, to produce fuel ethanol requires a microorganism that can ferment the mixture of sugars derived from hemicellulose. Escherichiacoli metabolizes a wide range of substrates and has been engineered to produce ethanol in high yield from sugar mixtures. E. coli metabolizes glucose in preference to other sugars and, as a result, utilization of the pentoses in hemicellulose-derived sugar mixtures is delayed and may be incomplete. Residual sugar lowers the ethanol yield and is problematic for downstream processing of fermentation products. Therefore, a catabolite repression mutant that simultaneously utilizes glucose and pentoses would be useful for fermentation of complex substrate mixtures. We constructed ethanologenic E. coli strains with a glucose phosphotransferase (ptsG) mutation and used the mutants to ferment glucose, arabinose, and xylose, singly and in mixtures, to ethanol. Yields were 87\u201394% of theoretical for both the wild type and mutants, but the mutants had an altered pattern of mixed sugar utilization. Phosphotransferase mutants metabolized the pentoses simultaneously with glucose, rather than sequentially. Based upon fermentations of sugar mixtures, a catabolite-repression mutant of ethanologenic E. coli is expected to provide more efficient fermentation of hemicellulose hydrolysates by allowing direct utilization of pentoses.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/s002530100628", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1083533", 
        "issn": [
          "0175-7598", 
          "1432-0614"
        ], 
        "name": "Applied Microbiology and Biotechnology", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "56"
      }
    ], 
    "keywords": [
      "catabolite repression mutants", 
      "ethanologenic E. coli", 
      "E. coli", 
      "sugar mixtures", 
      "complex substrate mixtures", 
      "mixed sugar utilization", 
      "wild type", 
      "mutants", 
      "efficient fermentation", 
      "ethanol yield", 
      "fermentation products", 
      "fuel ethanol", 
      "hemicellulose hydrolysate", 
      "residual sugar", 
      "sugar utilization", 
      "downstream processing", 
      "coli", 
      "pentose", 
      "fermentation", 
      "altered pattern", 
      "mixture of sugars", 
      "substrate mixture", 
      "agricultural biomass", 
      "sugars", 
      "ethanol", 
      "high yields", 
      "mutations", 
      "microorganisms", 
      "Escherichiacoli", 
      "direct utilization", 
      "biomass", 
      "arabinose", 
      "utilization", 
      "glucose", 
      "yield", 
      "xylose", 
      "wide range", 
      "substrate", 
      "hydrolysate", 
      "hemicellulose", 
      "patterns", 
      "mixture", 
      "range", 
      "processing", 
      "preferences", 
      "types", 
      "products", 
      "use", 
      "results", 
      "cornstarch"
    ], 
    "name": "Use of catabolite repression mutants for fermentation of sugar mixtures to ethanol", 
    "pagination": "120-125", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1031253667"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s002530100628"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "11499918"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s002530100628", 
      "https://app.dimensions.ai/details/publication/pub.1031253667"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-12-01T06:22", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20221201/entities/gbq_results/article/article_308.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/s002530100628"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s002530100628'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s002530100628'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s002530100628'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s002530100628'


 

This table displays all metadata directly associated to this object as RDF triples.

153 TRIPLES      20 PREDICATES      83 URIs      75 LITERALS      14 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s002530100628 schema:about N003d9bbf8b2e4182a9a183ecffa6ed1f
2 N105909a3454d4ea4b7097e27d3446ce5
3 N2229b34c7e274a76a135d67923482330
4 N37643fc29cb34370ae092bae81ff05a7
5 Ncdcb54a684804cb4955c2e0c238e71ba
6 Ne15fc269de794b509de6b175ccfae9e7
7 Ne4ad3af4d7c344ba90db4d398350dde5
8 anzsrc-for:06
9 anzsrc-for:0605
10 schema:author N1c1dc2b69b5846818e4877fe1ebcbaa9
11 schema:datePublished 2001-07
12 schema:datePublishedReg 2001-07-01
13 schema:description Abstract. Use of agricultural biomass, other than cornstarch, to produce fuel ethanol requires a microorganism that can ferment the mixture of sugars derived from hemicellulose. Escherichiacoli metabolizes a wide range of substrates and has been engineered to produce ethanol in high yield from sugar mixtures. E. coli metabolizes glucose in preference to other sugars and, as a result, utilization of the pentoses in hemicellulose-derived sugar mixtures is delayed and may be incomplete. Residual sugar lowers the ethanol yield and is problematic for downstream processing of fermentation products. Therefore, a catabolite repression mutant that simultaneously utilizes glucose and pentoses would be useful for fermentation of complex substrate mixtures. We constructed ethanologenic E. coli strains with a glucose phosphotransferase (ptsG) mutation and used the mutants to ferment glucose, arabinose, and xylose, singly and in mixtures, to ethanol. Yields were 87–94% of theoretical for both the wild type and mutants, but the mutants had an altered pattern of mixed sugar utilization. Phosphotransferase mutants metabolized the pentoses simultaneously with glucose, rather than sequentially. Based upon fermentations of sugar mixtures, a catabolite-repression mutant of ethanologenic E. coli is expected to provide more efficient fermentation of hemicellulose hydrolysates by allowing direct utilization of pentoses.
14 schema:genre article
15 schema:isAccessibleForFree false
16 schema:isPartOf N1076f79094124da6a363170b9185e86c
17 N282c259f15da455a814dfd3c609212df
18 sg:journal.1083533
19 schema:keywords E. coli
20 Escherichiacoli
21 agricultural biomass
22 altered pattern
23 arabinose
24 biomass
25 catabolite repression mutants
26 coli
27 complex substrate mixtures
28 cornstarch
29 direct utilization
30 downstream processing
31 efficient fermentation
32 ethanol
33 ethanol yield
34 ethanologenic E. coli
35 fermentation
36 fermentation products
37 fuel ethanol
38 glucose
39 hemicellulose
40 hemicellulose hydrolysate
41 high yields
42 hydrolysate
43 microorganisms
44 mixed sugar utilization
45 mixture
46 mixture of sugars
47 mutants
48 mutations
49 patterns
50 pentose
51 preferences
52 processing
53 products
54 range
55 residual sugar
56 results
57 substrate
58 substrate mixture
59 sugar mixtures
60 sugar utilization
61 sugars
62 types
63 use
64 utilization
65 wide range
66 wild type
67 xylose
68 yield
69 schema:name Use of catabolite repression mutants for fermentation of sugar mixtures to ethanol
70 schema:pagination 120-125
71 schema:productId N4e0329ad63bc45a7b831d57f36920472
72 N6e088f95d660469fa0ff9193bdbf5091
73 N723a53a451bd4c959f45d057e5843819
74 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031253667
75 https://doi.org/10.1007/s002530100628
76 schema:sdDatePublished 2022-12-01T06:22
77 schema:sdLicense https://scigraph.springernature.com/explorer/license/
78 schema:sdPublisher Na7606662ae5f400a8b1d378645a0b4fb
79 schema:url https://doi.org/10.1007/s002530100628
80 sgo:license sg:explorer/license/
81 sgo:sdDataset articles
82 rdf:type schema:ScholarlyArticle
83 N003d9bbf8b2e4182a9a183ecffa6ed1f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
84 schema:name Phosphoenolpyruvate Sugar Phosphotransferase System
85 rdf:type schema:DefinedTerm
86 N105909a3454d4ea4b7097e27d3446ce5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
87 schema:name Biomass
88 rdf:type schema:DefinedTerm
89 N1076f79094124da6a363170b9185e86c schema:volumeNumber 56
90 rdf:type schema:PublicationVolume
91 N1c1dc2b69b5846818e4877fe1ebcbaa9 rdf:first sg:person.01362477120.95
92 rdf:rest Nb4736069ba30473a97f9fe20a89805af
93 N2229b34c7e274a76a135d67923482330 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
94 schema:name Carbohydrate Metabolism
95 rdf:type schema:DefinedTerm
96 N282c259f15da455a814dfd3c609212df schema:issueNumber 1
97 rdf:type schema:PublicationIssue
98 N37643fc29cb34370ae092bae81ff05a7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Ethanol
100 rdf:type schema:DefinedTerm
101 N4e0329ad63bc45a7b831d57f36920472 schema:name pubmed_id
102 schema:value 11499918
103 rdf:type schema:PropertyValue
104 N6e088f95d660469fa0ff9193bdbf5091 schema:name doi
105 schema:value 10.1007/s002530100628
106 rdf:type schema:PropertyValue
107 N723a53a451bd4c959f45d057e5843819 schema:name dimensions_id
108 schema:value pub.1031253667
109 rdf:type schema:PropertyValue
110 N933f550aa8c245628c29f98ce3b2e2d3 rdf:first sg:person.077623511.44
111 rdf:rest rdf:nil
112 Na7606662ae5f400a8b1d378645a0b4fb schema:name Springer Nature - SN SciGraph project
113 rdf:type schema:Organization
114 Nb4736069ba30473a97f9fe20a89805af rdf:first sg:person.0643421433.32
115 rdf:rest N933f550aa8c245628c29f98ce3b2e2d3
116 Ncdcb54a684804cb4955c2e0c238e71ba schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
117 schema:name Mutation
118 rdf:type schema:DefinedTerm
119 Ne15fc269de794b509de6b175ccfae9e7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
120 schema:name Fermentation
121 rdf:type schema:DefinedTerm
122 Ne4ad3af4d7c344ba90db4d398350dde5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
123 schema:name Escherichia coli
124 rdf:type schema:DefinedTerm
125 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
126 schema:name Biological Sciences
127 rdf:type schema:DefinedTerm
128 anzsrc-for:0605 schema:inDefinedTermSet anzsrc-for:
129 schema:name Microbiology
130 rdf:type schema:DefinedTerm
131 sg:journal.1083533 schema:issn 0175-7598
132 1432-0614
133 schema:name Applied Microbiology and Biotechnology
134 schema:publisher Springer Nature
135 rdf:type schema:Periodical
136 sg:person.01362477120.95 schema:affiliation grid-institutes:grid.507311.1
137 schema:familyName Nichols
138 schema:givenName N.
139 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01362477120.95
140 rdf:type schema:Person
141 sg:person.0643421433.32 schema:affiliation grid-institutes:grid.507311.1
142 schema:familyName Dien
143 schema:givenName B.
144 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0643421433.32
145 rdf:type schema:Person
146 sg:person.077623511.44 schema:affiliation grid-institutes:grid.507311.1
147 schema:familyName Bothast
148 schema:givenName R.
149 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.077623511.44
150 rdf:type schema:Person
151 grid-institutes:grid.507311.1 schema:alternateName Fermentation Biochemistry Research Unit, National Center for Agricultural Utilization Research, USDA, Agricultural Research Service, 1815 N. University Street, Peoria, IL 61604, USA
152 schema:name Fermentation Biochemistry Research Unit, National Center for Agricultural Utilization Research, USDA, Agricultural Research Service, 1815 N. University Street, Peoria, IL 61604, USA
153 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...