Establishment of a gene transfer system for Rhodococcus opacus PD630 based on electroporation and its application for recombinant biosynthesis of ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1999-10

AUTHORS

R. Kalscheuer, M. Arenskötter, A. Steinbüchel

ABSTRACT

A gene transfer system for Rhodococcus opacus PD630 based on electroporation was established and optimized employing the Escherichia coli-Rhodococcus shuttle vectors pNC9501 and pNC9503 as well as the E. coli-Corynebacterium glutamicum shuttle vector pJC1 as suitable cloning vectors for R. opacus PD630, resulting in transformation efficiencies up to 1.5 x 10(5) CFUs/microgram plasmid DNA. Applying the optimized electroporation protocol to the pNC9501-derivatives pAK68 and pAK71 harboring the entire PHB synthesis operon from Ralstonia eutropha and the PHA synthase gene phaC1 from Pseudomonas aeruginosa, respectively, recombinant PHA biosynthesis was established in R. opacus PD630 and the TAG-negative mutant ROM34. Plasmid pAK68 enabled synthesis and accumulation of poly(3HB) in R. opacus PD630 and ROM34 during cultivation under storage conditions from 1% (w/v) gluconate, of poly(3HB-co-3HV) from 0.2% (w/v) propionate and of poly(3HV) from 0.1% (w/v) valerate. Under storage conditions, recombinant strains of PD630 and ROM34 harboring pAK71 were able to synthesize and accumulate PHA of the medium chain length hydroxyalkanoic acids 3HHx, 3HO, 3HD and 3HDD from 0.1% (w/v) hexadecane or octadecane and a copolyester composed of 3HHp, 3HN and 3HUD from 0.1% (w/v) pentadecane or heptadecane. In the recombinant strains of PD630 and ROM34, the thiostrepton-induced overexpression of a 20 kDa protein was observed with its N-terminus exhibiting a homology of 60% identical amino acids to TipA from Streptomyces lividans. More... »

PAGES

508-515

Journal

TITLE

Applied Microbiology and Biotechnology

ISSUE

4

VOLUME

52

Author Affiliations

Related Patents

  • Recombinant Microalgae Cells Producing Novel Oils
  • Recombinant Microalgae Including Keto-Acyl Acp Synthase
  • Thioesterases And Cells For Production Of Tailored Oils
  • Oleaginous Bacterial Cells And Methods For Producing Lipids
  • Variant Thioesterases And Methods Of Use
  • Thioesterases And Cells For Production Of Tailored Oils
  • Methods Of Producing Triacylglyceride Compositions Comprising Tailored Oils
  • Tailored Oils Produced From Recombinant Heterotrophic Microorganisms
  • Tailored Oils
  • Renewable Chemical Production From Novel Fatty Acid Feedstocks
  • Tailored Oils
  • Genetically Engineered Microbial Strains Including Prototheca Lipid Pathway Genes
  • Production Of Hydroxylated Fatty Acids In Prototheca Moriformis
  • Pyrolysis Oil And Other Combustible Compositions From Microbial Biomass
  • Variant Thioesterases And Methods Of Use
  • Microbial Oils With Lowered Pour Points, Dielectric Fluids Produced Therefrom, And Related Methods
  • Production Of Oil In Microorganisms
  • Methods For Producing A Triglyceride Composition From Algae
  • Hydroxylated Triacylglycerides
  • Genetically Engineered Microbial Strains Including Prototheca Lipid Pathway Genes
  • Recombinant Microalgae Cells Producing Novel Oils
  • Genetically Engineered Microorganisms That Metabolize Xylose
  • Ketoacyl Acp Synthase Genes And Uses Thereof
  • Renewable Fuels Produced From Oleaginous Microorganisms
  • Heterotrophic Microalgae Expressing Invertase
  • Nucleic Acids Useful In The Manufacture Of Oil
  • Recombinant Microalgae Including Sucrose Invertase And Thioesterase
  • Recombinant Microalgae Cells Producing Novel Oils
  • Genetically Engineered Microorganisms That Metabolize Xylose
  • Production Of Oil In Microorganisms
  • Microorganisms Expressing Ketoacyl-Coa Synthase And Uses Thereof
  • Structuring Fats And Methods Of Producing Structuring Fats
  • Nucleic Acids Useful In The Manufacture Of Oil
  • Tailored Oils Produced From Recombinant Heterotrophic Microorganisms
  • Tailored Oils Produced From Recombinant Heterotrophic Microorganisms
  • Genetically Engineered Microbial Strains Including Chlorella Protothecoides Lipid Pathway Genes
  • Renewable Diesel And Jet Fuel From Microbial Sources
  • Tailored Oils
  • Tailored Oils
  • Tailored Oils
  • Chlorella Species Containing Exogenous Genes
  • Production Of Oil In Microorganisms
  • Renewable Chemical Production From Novel Fatty Acid Feedstocks
  • Tailored Oils Produced From Recombinant Oleaginous Microorganisms
  • Structuring Fats And Methods Of Producing Structuring Fats
  • Tailored Oils
  • Tailored Oils
  • Tailored Oils Produced From Recombinant Heterotrophic Microorganisms
  • Recombinant Microalgae Cells Producing Novel Oils
  • Variant Thioesterases And Methods Of Use
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s002530051553

    DOI

    http://dx.doi.org/10.1007/s002530051553

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1027492239

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/10570798


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Acyltransferases", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Amino Acid Sequence", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Bacterial Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Chromatography, Gas", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Culture Media", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Electrophoresis, Polyacrylamide Gel", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Electroporation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Transfer Techniques", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genes, Bacterial", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genetic Vectors", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Molecular Sequence Data", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mutation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Pseudomonas aeruginosa", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Recombinant Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Rhodococcus", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Thiostrepton", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Trans-Activators", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Triglycerides", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "University of M\u00fcnster", 
              "id": "https://www.grid.ac/institutes/grid.5949.1", 
              "name": [
                "Institut f\u00fcr Mikrobiologie, Westf\u00e4lische Wilhelms-Universit\u00e4t M\u00fcnster, Corrensstrasse 3, D-48149 M\u00fcnster, Germany e-mail: steinbu@uni-muenster.de Tel.: +49-251-8339821 Fax: +49-251-8338388, DE"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Kalscheuer", 
            "givenName": "R.", 
            "id": "sg:person.01066703776.89", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01066703776.89"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of M\u00fcnster", 
              "id": "https://www.grid.ac/institutes/grid.5949.1", 
              "name": [
                "Institut f\u00fcr Mikrobiologie, Westf\u00e4lische Wilhelms-Universit\u00e4t M\u00fcnster, Corrensstrasse 3, D-48149 M\u00fcnster, Germany e-mail: steinbu@uni-muenster.de Tel.: +49-251-8339821 Fax: +49-251-8338388, DE"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Arensk\u00f6tter", 
            "givenName": "M.", 
            "id": "sg:person.01322673464.77", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01322673464.77"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of M\u00fcnster", 
              "id": "https://www.grid.ac/institutes/grid.5949.1", 
              "name": [
                "Institut f\u00fcr Mikrobiologie, Westf\u00e4lische Wilhelms-Universit\u00e4t M\u00fcnster, Corrensstrasse 3, D-48149 M\u00fcnster, Germany e-mail: steinbu@uni-muenster.de Tel.: +49-251-8339821 Fax: +49-251-8338388, DE"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Steinb\u00fcchel", 
            "givenName": "A.", 
            "id": "sg:person.0673051146.21", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0673051146.21"
            ], 
            "type": "Person"
          }
        ], 
        "datePublished": "1999-10", 
        "datePublishedReg": "1999-10-01", 
        "description": "A gene transfer system for Rhodococcus opacus PD630 based on electroporation was established and optimized employing the Escherichia coli-Rhodococcus shuttle vectors pNC9501 and pNC9503 as well as the E. coli-Corynebacterium glutamicum shuttle vector pJC1 as suitable cloning vectors for R. opacus PD630, resulting in transformation efficiencies up to 1.5 x 10(5) CFUs/microgram plasmid DNA. Applying the optimized electroporation protocol to the pNC9501-derivatives pAK68 and pAK71 harboring the entire PHB synthesis operon from Ralstonia eutropha and the PHA synthase gene phaC1 from Pseudomonas aeruginosa, respectively, recombinant PHA biosynthesis was established in R. opacus PD630 and the TAG-negative mutant ROM34. Plasmid pAK68 enabled synthesis and accumulation of poly(3HB) in R. opacus PD630 and ROM34 during cultivation under storage conditions from 1% (w/v) gluconate, of poly(3HB-co-3HV) from 0.2% (w/v) propionate and of poly(3HV) from 0.1% (w/v) valerate. Under storage conditions, recombinant strains of PD630 and ROM34 harboring pAK71 were able to synthesize and accumulate PHA of the medium chain length hydroxyalkanoic acids 3HHx, 3HO, 3HD and 3HDD from 0.1% (w/v) hexadecane or octadecane and a copolyester composed of 3HHp, 3HN and 3HUD from 0.1% (w/v) pentadecane or heptadecane. In the recombinant strains of PD630 and ROM34, the thiostrepton-induced overexpression of a 20 kDa protein was observed with its N-terminus exhibiting a homology of 60% identical amino acids to TipA from Streptomyces lividans.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1007/s002530051553", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1083533", 
            "issn": [
              "0175-7598", 
              "1432-0614"
            ], 
            "name": "Applied Microbiology and Biotechnology", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "4", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "52"
          }
        ], 
        "name": "Establishment of a gene transfer system for Rhodococcus opacus PD630 based on electroporation and its application for recombinant biosynthesis of poly(3-hydroxyalkanoic acids)", 
        "pagination": "508-515", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "82f6c386a9e5ca0a9603d34eaa567c2a258159a6182e4b340a68cababf4bdee1"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "10570798"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "8406612"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/s002530051553"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1027492239"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/s002530051553", 
          "https://app.dimensions.ai/details/publication/pub.1027492239"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T15:52", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8664_00000513.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://link.springer.com/10.1007%2Fs002530051553"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s002530051553'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s002530051553'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s002530051553'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s002530051553'


     

    This table displays all metadata directly associated to this object as RDF triples.

    155 TRIPLES      20 PREDICATES      47 URIs      39 LITERALS      27 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/s002530051553 schema:about N1689fca556134570a9c2af0a0bd2e68e
    2 N1e4773566c574cbca4cab075626037bc
    3 N20262d31d63e4f289d3b916a214455cc
    4 N292509b38b904666adf4e4185380f5a9
    5 N4209aab5c0054925bd7d9c7ef36491d2
    6 N47aeeb2a219740f18fe9c80353b24d68
    7 N6c9ccc95939e4a839637e53c47c59621
    8 N7a69bdb6357b47f085733096c0d4cda0
    9 N7f0d7f5f116e4721842173e54323e813
    10 N82dae2c5707a40c7a79f892fa12dc810
    11 Na81c448baaef49bf87336395f3ae2af4
    12 Nb3032c22f1fd4834893158071a305b7b
    13 Nc5e3eddfb73a4d2e8061610bd6bdda1d
    14 Nc7b5619e285541ebb79d6e24eefba6fd
    15 Nd3ba769096ec4976b2fd5d6ee4fb3372
    16 Nda0b7b4a35eb4bb2ac721b1a9e431cf3
    17 Ne4e61973f5804b79857e39c07df6d8b8
    18 Ne815b7c546644297b337e4a6ee6a3c49
    19 anzsrc-for:06
    20 anzsrc-for:0601
    21 schema:author Ncd280487112e4bac9d256785b323fffb
    22 schema:datePublished 1999-10
    23 schema:datePublishedReg 1999-10-01
    24 schema:description A gene transfer system for Rhodococcus opacus PD630 based on electroporation was established and optimized employing the Escherichia coli-Rhodococcus shuttle vectors pNC9501 and pNC9503 as well as the E. coli-Corynebacterium glutamicum shuttle vector pJC1 as suitable cloning vectors for R. opacus PD630, resulting in transformation efficiencies up to 1.5 x 10(5) CFUs/microgram plasmid DNA. Applying the optimized electroporation protocol to the pNC9501-derivatives pAK68 and pAK71 harboring the entire PHB synthesis operon from Ralstonia eutropha and the PHA synthase gene phaC1 from Pseudomonas aeruginosa, respectively, recombinant PHA biosynthesis was established in R. opacus PD630 and the TAG-negative mutant ROM34. Plasmid pAK68 enabled synthesis and accumulation of poly(3HB) in R. opacus PD630 and ROM34 during cultivation under storage conditions from 1% (w/v) gluconate, of poly(3HB-co-3HV) from 0.2% (w/v) propionate and of poly(3HV) from 0.1% (w/v) valerate. Under storage conditions, recombinant strains of PD630 and ROM34 harboring pAK71 were able to synthesize and accumulate PHA of the medium chain length hydroxyalkanoic acids 3HHx, 3HO, 3HD and 3HDD from 0.1% (w/v) hexadecane or octadecane and a copolyester composed of 3HHp, 3HN and 3HUD from 0.1% (w/v) pentadecane or heptadecane. In the recombinant strains of PD630 and ROM34, the thiostrepton-induced overexpression of a 20 kDa protein was observed with its N-terminus exhibiting a homology of 60% identical amino acids to TipA from Streptomyces lividans.
    25 schema:genre research_article
    26 schema:inLanguage en
    27 schema:isAccessibleForFree false
    28 schema:isPartOf N1135166d47a84112919cdfb0628b6e84
    29 N1cc512824da94547bfd0e078d9ad51a9
    30 sg:journal.1083533
    31 schema:name Establishment of a gene transfer system for Rhodococcus opacus PD630 based on electroporation and its application for recombinant biosynthesis of poly(3-hydroxyalkanoic acids)
    32 schema:pagination 508-515
    33 schema:productId N134f5024f0794de2b4c62d7f74459ebb
    34 N32d36eb4143a4635b6af148a48097a1b
    35 N683d516d9e59473f90bf53f0f09d5d7c
    36 N8380f521bd82400e90ed7e294cf49c54
    37 Nf71dc31b0f404c31add17cb7960618e5
    38 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027492239
    39 https://doi.org/10.1007/s002530051553
    40 schema:sdDatePublished 2019-04-10T15:52
    41 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    42 schema:sdPublisher Ne2a1ccad7e404dffbf867b54672a9cb2
    43 schema:url http://link.springer.com/10.1007%2Fs002530051553
    44 sgo:license sg:explorer/license/
    45 sgo:sdDataset articles
    46 rdf:type schema:ScholarlyArticle
    47 N1135166d47a84112919cdfb0628b6e84 schema:volumeNumber 52
    48 rdf:type schema:PublicationVolume
    49 N134f5024f0794de2b4c62d7f74459ebb schema:name pubmed_id
    50 schema:value 10570798
    51 rdf:type schema:PropertyValue
    52 N1689fca556134570a9c2af0a0bd2e68e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    53 schema:name Bacterial Proteins
    54 rdf:type schema:DefinedTerm
    55 N1b1ba6a3d88940d2a03dd769efd8db2f rdf:first sg:person.01322673464.77
    56 rdf:rest Ne65a581c22be40f8a53a36a03e6c029d
    57 N1cc512824da94547bfd0e078d9ad51a9 schema:issueNumber 4
    58 rdf:type schema:PublicationIssue
    59 N1e4773566c574cbca4cab075626037bc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    60 schema:name Pseudomonas aeruginosa
    61 rdf:type schema:DefinedTerm
    62 N20262d31d63e4f289d3b916a214455cc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    63 schema:name Rhodococcus
    64 rdf:type schema:DefinedTerm
    65 N292509b38b904666adf4e4185380f5a9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    66 schema:name Acyltransferases
    67 rdf:type schema:DefinedTerm
    68 N32d36eb4143a4635b6af148a48097a1b schema:name readcube_id
    69 schema:value 82f6c386a9e5ca0a9603d34eaa567c2a258159a6182e4b340a68cababf4bdee1
    70 rdf:type schema:PropertyValue
    71 N4209aab5c0054925bd7d9c7ef36491d2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    72 schema:name Genetic Vectors
    73 rdf:type schema:DefinedTerm
    74 N47aeeb2a219740f18fe9c80353b24d68 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    75 schema:name Genes, Bacterial
    76 rdf:type schema:DefinedTerm
    77 N683d516d9e59473f90bf53f0f09d5d7c schema:name dimensions_id
    78 schema:value pub.1027492239
    79 rdf:type schema:PropertyValue
    80 N6c9ccc95939e4a839637e53c47c59621 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    81 schema:name Thiostrepton
    82 rdf:type schema:DefinedTerm
    83 N7a69bdb6357b47f085733096c0d4cda0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    84 schema:name Triglycerides
    85 rdf:type schema:DefinedTerm
    86 N7f0d7f5f116e4721842173e54323e813 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    87 schema:name Molecular Sequence Data
    88 rdf:type schema:DefinedTerm
    89 N82dae2c5707a40c7a79f892fa12dc810 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    90 schema:name Culture Media
    91 rdf:type schema:DefinedTerm
    92 N8380f521bd82400e90ed7e294cf49c54 schema:name doi
    93 schema:value 10.1007/s002530051553
    94 rdf:type schema:PropertyValue
    95 Na81c448baaef49bf87336395f3ae2af4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    96 schema:name Trans-Activators
    97 rdf:type schema:DefinedTerm
    98 Nb3032c22f1fd4834893158071a305b7b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    99 schema:name Electrophoresis, Polyacrylamide Gel
    100 rdf:type schema:DefinedTerm
    101 Nc5e3eddfb73a4d2e8061610bd6bdda1d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    102 schema:name Gene Transfer Techniques
    103 rdf:type schema:DefinedTerm
    104 Nc7b5619e285541ebb79d6e24eefba6fd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    105 schema:name Mutation
    106 rdf:type schema:DefinedTerm
    107 Ncd280487112e4bac9d256785b323fffb rdf:first sg:person.01066703776.89
    108 rdf:rest N1b1ba6a3d88940d2a03dd769efd8db2f
    109 Nd3ba769096ec4976b2fd5d6ee4fb3372 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    110 schema:name Chromatography, Gas
    111 rdf:type schema:DefinedTerm
    112 Nda0b7b4a35eb4bb2ac721b1a9e431cf3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    113 schema:name Recombinant Proteins
    114 rdf:type schema:DefinedTerm
    115 Ne2a1ccad7e404dffbf867b54672a9cb2 schema:name Springer Nature - SN SciGraph project
    116 rdf:type schema:Organization
    117 Ne4e61973f5804b79857e39c07df6d8b8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    118 schema:name Electroporation
    119 rdf:type schema:DefinedTerm
    120 Ne65a581c22be40f8a53a36a03e6c029d rdf:first sg:person.0673051146.21
    121 rdf:rest rdf:nil
    122 Ne815b7c546644297b337e4a6ee6a3c49 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    123 schema:name Amino Acid Sequence
    124 rdf:type schema:DefinedTerm
    125 Nf71dc31b0f404c31add17cb7960618e5 schema:name nlm_unique_id
    126 schema:value 8406612
    127 rdf:type schema:PropertyValue
    128 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    129 schema:name Biological Sciences
    130 rdf:type schema:DefinedTerm
    131 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    132 schema:name Biochemistry and Cell Biology
    133 rdf:type schema:DefinedTerm
    134 sg:journal.1083533 schema:issn 0175-7598
    135 1432-0614
    136 schema:name Applied Microbiology and Biotechnology
    137 rdf:type schema:Periodical
    138 sg:person.01066703776.89 schema:affiliation https://www.grid.ac/institutes/grid.5949.1
    139 schema:familyName Kalscheuer
    140 schema:givenName R.
    141 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01066703776.89
    142 rdf:type schema:Person
    143 sg:person.01322673464.77 schema:affiliation https://www.grid.ac/institutes/grid.5949.1
    144 schema:familyName Arenskötter
    145 schema:givenName M.
    146 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01322673464.77
    147 rdf:type schema:Person
    148 sg:person.0673051146.21 schema:affiliation https://www.grid.ac/institutes/grid.5949.1
    149 schema:familyName Steinbüchel
    150 schema:givenName A.
    151 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0673051146.21
    152 rdf:type schema:Person
    153 https://www.grid.ac/institutes/grid.5949.1 schema:alternateName University of Münster
    154 schema:name Institut für Mikrobiologie, Westfälische Wilhelms-Universität Münster, Corrensstrasse 3, D-48149 Münster, Germany e-mail: steinbu@uni-muenster.de Tel.: +49-251-8339821 Fax: +49-251-8338388, DE
    155 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...