Mutants of Pseudomonas putida affected in poly-3-hydroxyalkanoate synthesis View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1998-06

AUTHORS

Q. Ren, B. Kessler, F. van der Leij, B. Witholt

ABSTRACT

The generation and characterization of Pseudomonas putida KT2442 mutants affected in poly-3-hydroxyalkanoate (PHA) synthesis are reported. The mutants from P. putida KT2442 carrying several copies of the PHA-polymerase-encoding gene (phaC) were isolated via N-methyl-N′-nitro-N-nitrosoguanidine chemical mutagenesis and contained mutation(s) on genes that are involved in PHA accumulation other than the phaC genes. No PHA-free mutants were obtained, suggesting that there must be various routes for the synthesis of PHA polymerase precursors. One of the isolated mutants (GPp120) accumulated more PHA than the parental strain, and there was virtually no down-regulation of PHA formation by growth in non-limiting amounts of nitrogen, which normally block or reduce formation of PHA. Compared to the parental strain, GPp120 exhibited significant changes in physiology and morphology when grown in minimal medium: the growth rate was reduced more than twofold and cells formed filaments. The other four groups of isolated mutants, with P. putida strains GPp121 to GPp124 as characteristic type strains, exhibited morphological characteristics similar to those of the parental strain. However, they showed reduced PHA production compared to the parental PHA+ strain, and especially GPp121 and GPp122 showed PHA formation tightly controlled by nutrient conditions. All of these mutants provide starting points for genetically dissecting the biosynthesis and regulation of PHA precursors. More... »

PAGES

743-750

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s002530051241

DOI

http://dx.doi.org/10.1007/s002530051241

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1036208138


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Swiss Federal Institute of Technology in Zurich", 
          "id": "https://www.grid.ac/institutes/grid.5801.c", 
          "name": [
            "Institute of Biotechnology, ETH H\u00f6nggerberg, CH-8093 Z\u00fcrich, Switzerland Tel.: +41 1 6333286 Fax: +41 1 6331051 e-mail: bw@biotech.biol.ethz.ch, CH"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ren", 
        "givenName": "Q.", 
        "id": "sg:person.0720347044.93", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0720347044.93"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Swiss Federal Institute of Technology in Zurich", 
          "id": "https://www.grid.ac/institutes/grid.5801.c", 
          "name": [
            "Institute of Biotechnology, ETH H\u00f6nggerberg, CH-8093 Z\u00fcrich, Switzerland Tel.: +41 1 6333286 Fax: +41 1 6331051 e-mail: bw@biotech.biol.ethz.ch, CH"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kessler", 
        "givenName": "B.", 
        "id": "sg:person.01006716074.42", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01006716074.42"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Swiss Federal Institute of Technology in Zurich", 
          "id": "https://www.grid.ac/institutes/grid.5801.c", 
          "name": [
            "Institute of Biotechnology, ETH H\u00f6nggerberg, CH-8093 Z\u00fcrich, Switzerland Tel.: +41 1 6333286 Fax: +41 1 6331051 e-mail: bw@biotech.biol.ethz.ch, CH"
          ], 
          "type": "Organization"
        }, 
        "familyName": "van der Leij", 
        "givenName": "F.", 
        "id": "sg:person.0657527027.75", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0657527027.75"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Swiss Federal Institute of Technology in Zurich", 
          "id": "https://www.grid.ac/institutes/grid.5801.c", 
          "name": [
            "Institute of Biotechnology, ETH H\u00f6nggerberg, CH-8093 Z\u00fcrich, Switzerland Tel.: +41 1 6333286 Fax: +41 1 6331051 e-mail: bw@biotech.biol.ethz.ch, CH"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Witholt", 
        "givenName": "B.", 
        "id": "sg:person.01014564664.63", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01014564664.63"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "1998-06", 
    "datePublishedReg": "1998-06-01", 
    "description": "The generation and characterization of Pseudomonas putida KT2442 mutants affected in poly-3-hydroxyalkanoate (PHA) synthesis are reported. The mutants from P. putida KT2442 carrying several copies of the PHA-polymerase-encoding gene (phaC) were isolated via N-methyl-N\u2032-nitro-N-nitrosoguanidine chemical mutagenesis and contained mutation(s) on genes that are involved in PHA accumulation other than the phaC genes. No PHA-free mutants were obtained, suggesting that there must be various routes for the synthesis of PHA polymerase precursors. One of the isolated mutants (GPp120) accumulated more PHA than the parental strain, and there was virtually no down-regulation of PHA formation by growth in non-limiting amounts of nitrogen, which normally block or reduce formation of PHA. Compared to the parental strain, GPp120 exhibited significant changes in physiology and morphology when grown in minimal medium: the growth rate was reduced more than twofold and cells formed filaments. The other four groups of isolated mutants, with P. putida strains GPp121 to GPp124 as characteristic type strains, exhibited morphological characteristics similar to those of the parental strain. However, they showed reduced PHA production compared to the parental PHA+ strain, and especially GPp121 and GPp122 showed PHA formation tightly controlled by nutrient conditions. All of these mutants provide starting points for genetically dissecting the biosynthesis and regulation of PHA precursors.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s002530051241", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1083533", 
        "issn": [
          "0175-7598", 
          "1432-0614"
        ], 
        "name": "Applied Microbiology and Biotechnology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "6", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "49"
      }
    ], 
    "name": "Mutants of Pseudomonas putida affected in poly-3-hydroxyalkanoate synthesis", 
    "pagination": "743-750", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "ac7ad7eb819045be793ee759af736d357cddb2313041298ae89e84168244d864"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s002530051241"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1036208138"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s002530051241", 
      "https://app.dimensions.ai/details/publication/pub.1036208138"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T16:43", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8669_00000514.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007%2Fs002530051241"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s002530051241'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s002530051241'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s002530051241'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s002530051241'


 

This table displays all metadata directly associated to this object as RDF triples.

82 TRIPLES      20 PREDICATES      27 URIs      19 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s002530051241 schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author N22913c4342644fe693fab1115d024cee
4 schema:datePublished 1998-06
5 schema:datePublishedReg 1998-06-01
6 schema:description The generation and characterization of Pseudomonas putida KT2442 mutants affected in poly-3-hydroxyalkanoate (PHA) synthesis are reported. The mutants from P. putida KT2442 carrying several copies of the PHA-polymerase-encoding gene (phaC) were isolated via N-methyl-N′-nitro-N-nitrosoguanidine chemical mutagenesis and contained mutation(s) on genes that are involved in PHA accumulation other than the phaC genes. No PHA-free mutants were obtained, suggesting that there must be various routes for the synthesis of PHA polymerase precursors. One of the isolated mutants (GPp120) accumulated more PHA than the parental strain, and there was virtually no down-regulation of PHA formation by growth in non-limiting amounts of nitrogen, which normally block or reduce formation of PHA. Compared to the parental strain, GPp120 exhibited significant changes in physiology and morphology when grown in minimal medium: the growth rate was reduced more than twofold and cells formed filaments. The other four groups of isolated mutants, with P. putida strains GPp121 to GPp124 as characteristic type strains, exhibited morphological characteristics similar to those of the parental strain. However, they showed reduced PHA production compared to the parental PHA+ strain, and especially GPp121 and GPp122 showed PHA formation tightly controlled by nutrient conditions. All of these mutants provide starting points for genetically dissecting the biosynthesis and regulation of PHA precursors.
7 schema:genre research_article
8 schema:inLanguage en
9 schema:isAccessibleForFree false
10 schema:isPartOf N526d99bfb03246239356e3b42707c994
11 N92ecb4ce89bc4230baee002ce25a4828
12 sg:journal.1083533
13 schema:name Mutants of Pseudomonas putida affected in poly-3-hydroxyalkanoate synthesis
14 schema:pagination 743-750
15 schema:productId N5717cf87499949d88e9cd14b18c908b6
16 N57dcfb5aed1c4f56975505d1db0df6bf
17 Ne955f27388cb440f81ebbf7ee5e38920
18 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036208138
19 https://doi.org/10.1007/s002530051241
20 schema:sdDatePublished 2019-04-10T16:43
21 schema:sdLicense https://scigraph.springernature.com/explorer/license/
22 schema:sdPublisher N5233edf23f3e4f0ea0e2b512b3e7a97e
23 schema:url http://link.springer.com/10.1007%2Fs002530051241
24 sgo:license sg:explorer/license/
25 sgo:sdDataset articles
26 rdf:type schema:ScholarlyArticle
27 N22913c4342644fe693fab1115d024cee rdf:first sg:person.0720347044.93
28 rdf:rest Nd865832900d7476bbc567c444828f218
29 N5233edf23f3e4f0ea0e2b512b3e7a97e schema:name Springer Nature - SN SciGraph project
30 rdf:type schema:Organization
31 N526d99bfb03246239356e3b42707c994 schema:issueNumber 6
32 rdf:type schema:PublicationIssue
33 N5717cf87499949d88e9cd14b18c908b6 schema:name dimensions_id
34 schema:value pub.1036208138
35 rdf:type schema:PropertyValue
36 N57dcfb5aed1c4f56975505d1db0df6bf schema:name doi
37 schema:value 10.1007/s002530051241
38 rdf:type schema:PropertyValue
39 N609c82d817da4f5ea6b62df1b75ff779 rdf:first sg:person.01014564664.63
40 rdf:rest rdf:nil
41 N92ecb4ce89bc4230baee002ce25a4828 schema:volumeNumber 49
42 rdf:type schema:PublicationVolume
43 N9983fbf1836445deb33c957a7dd4de90 rdf:first sg:person.0657527027.75
44 rdf:rest N609c82d817da4f5ea6b62df1b75ff779
45 Nd865832900d7476bbc567c444828f218 rdf:first sg:person.01006716074.42
46 rdf:rest N9983fbf1836445deb33c957a7dd4de90
47 Ne955f27388cb440f81ebbf7ee5e38920 schema:name readcube_id
48 schema:value ac7ad7eb819045be793ee759af736d357cddb2313041298ae89e84168244d864
49 rdf:type schema:PropertyValue
50 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
51 schema:name Biological Sciences
52 rdf:type schema:DefinedTerm
53 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
54 schema:name Biochemistry and Cell Biology
55 rdf:type schema:DefinedTerm
56 sg:journal.1083533 schema:issn 0175-7598
57 1432-0614
58 schema:name Applied Microbiology and Biotechnology
59 rdf:type schema:Periodical
60 sg:person.01006716074.42 schema:affiliation https://www.grid.ac/institutes/grid.5801.c
61 schema:familyName Kessler
62 schema:givenName B.
63 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01006716074.42
64 rdf:type schema:Person
65 sg:person.01014564664.63 schema:affiliation https://www.grid.ac/institutes/grid.5801.c
66 schema:familyName Witholt
67 schema:givenName B.
68 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01014564664.63
69 rdf:type schema:Person
70 sg:person.0657527027.75 schema:affiliation https://www.grid.ac/institutes/grid.5801.c
71 schema:familyName van der Leij
72 schema:givenName F.
73 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0657527027.75
74 rdf:type schema:Person
75 sg:person.0720347044.93 schema:affiliation https://www.grid.ac/institutes/grid.5801.c
76 schema:familyName Ren
77 schema:givenName Q.
78 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0720347044.93
79 rdf:type schema:Person
80 https://www.grid.ac/institutes/grid.5801.c schema:alternateName Swiss Federal Institute of Technology in Zurich
81 schema:name Institute of Biotechnology, ETH Hönggerberg, CH-8093 Zürich, Switzerland Tel.: +41 1 6333286 Fax: +41 1 6331051 e-mail: bw@biotech.biol.ethz.ch, CH
82 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...