Catabolite-repressor-like protein regulates the expression of a gene under the control of the Escherichia coli lac promoter in the plant ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1996-12

AUTHORS

S. D. Soby, M. J. Daniels

ABSTRACT

Xanthomonas campestris pv. campestris, the causal agent of black-rot disease of cruciferous plants, and an important industrial microbe, was able to express the Escherichia coli beta-glucuronidase reporter gene (uidA) when fused to the E. coli lactose operon promoter on a wide-host-range plasmid vector. The gene fusion is expressed constitutively at high levels in both complex and defined media using a wide range of carbon sources, and is not repressible by glucose or inducible by the gratuitous lac inducer isopropyl beta-D-thiogalactoside. An X. campestris campestris strain with a lesion in the clp (catabolite-repressor-like protein) locus, and containing the placluidA fusion, was tested for beta-glucuronidase activity. We found that the expression of the placluidA fusion gene is dependent on the presence of catabolite-repressor-like protein, with an approximately 75% reduction of expression in the clp -deficient mutant. More... »

PAGES

559-561

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s002530050861

DOI

http://dx.doi.org/10.1007/s002530050861

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1007282240

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/9008890


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