The effect of osmotic pressure on the membrane fluidity of Saccharomyces cerevisiae at different physiological temperatures View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2001-07

AUTHORS

C. Laroche, L. Beney, P. Marechal, P. Gervais

ABSTRACT

Membrane fluidity in whole cells of Saccharomyces cerevisiae W303-1A was estimated from fluorescence polarization measurements using the membrane probe, 1,6-diphenyl-1,3,5-hexatriene, over a wide range of temperatures (6-35 degrees C) and at seven levels of osmotic pressure between 1.38 MPa and 133.1 MPa. An increase in phase transition temperatures was observed with increasing osmotic pressure. At 1.38 MPa, a phase transition temperature of 12 +/- 2 degrees C was observed, which increased to 17 +/- 4 degrees C at 43.7 MPa, 21+/- 7 degrees C at 61.8 MPa, and 24 +/- 9 degrees C at an osmotic pressure of 133.1 MPa. From these results we infer that, with increases in osmotic pressure, the change in phospholipid conformation occurs over a larger temperature range. These results allow the representation of membrane fluidity as a function of temperature and osmotic pressure. Osmotic shocks were applied at two levels of osmotic pressure and at nine temperatures, in order to relate membrane conformation to cell viability. More... »

PAGES

249-254

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s002530000583

DOI

http://dx.doi.org/10.1007/s002530000583

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1035973675

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/11499939


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