A genetically modified solvent-tolerant bacterium for optimized production of a toxic fine chemical View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2000-08

AUTHORS

J. Wery, D. I. Mendes da Silva, J. A. M. de Bont

ABSTRACT

The aim of the study was to investigate whether toxic fine chemical production can be improved using the solvent-tolerant Pseudomonas putida S12 in a two-liquid-phase system consisting of aqueous media and a water-immiscible octanol phase with production of 3-methylcatechol from toluene as the model conversion. For this purpose the genes involved in this conversion, todC1C2BAD from P. putida F1, were introduced into P. putida S12 with high stable expression. Production of 3-methylcatechol was monitored in batch incubations with different media using a single medium and a two-liquid medium-octanol system. The maximum concentration of 3-methylcatechol increased two-fold using the two-liquid medium-octanol system, irrespective of the selected medium. More... »

PAGES

180-185

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s002530000381

DOI

http://dx.doi.org/10.1007/s002530000381

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1017934418

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/10968630


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