Biotic elicitor–induced changes in growth, antioxidative defense, and metabolites in an improved prickleless Solanum viarum View Full Text


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Article Info

DATE

2022-09-07

AUTHORS

Archana Prasad, Preeti Patel, Abhishek Niranjan, Aradhana Mishra, Gauri Saxena, Satya Shila Singh, Debasis Chakrabarty

ABSTRACT

Solanum viarum serves as a raw material for the steroidal drug industry due to its alkaloid and glycoalkaloid content. Elicitation is well-known for measuring the increase in the yield of bioactive compounds in in vitro cultures. The current study was performed for the accumulation of metabolites viz. solasodine, solanidine, and α-solanine in S. viarum culture using microbial-based elicitors added in 1%, 3%, 5%, and 7% on 25th and 35th day of culture period and harvested on 45th and 50th days of culture cycle. The treatment of 3% Trichoderma reesei and Bacillus tequilensis culture filtrate (CF) significantly increased biomass, alkaloids/glycoalkaloid content, and yield in S. viarum. T. reesei was found to be the best treatment for enhanced growth (GI = 11.65) and glycoalkaloid yield (2.54 mg DW plant−1) after the 50th day of the culture cycle when added on the 25th day. The abundance of gene transcripts involved in the biosynthesis of alkaloids/glycoalkaloids, revealed by quantitative real-time PCR expression analysis correlates with the accumulation of their respective metabolites in elicited plants. Biochemical analysis shows that elicited plants inhibited oxidative damage caused by reactive oxygen species by activating enzymes (superoxide dismutase and ascorbate peroxidase) as well as non-enzymatic antioxidant mechanisms (alkaloids, total phenols, total flavonoids, carotenoids, and proline). The findings of this study clearly demonstrate that the application of T. reesei and B. tequilensis CF at a specific dose and time significantly improve biomass as well as upregulates the metabolite biosynthetic pathway in an important medicinal plant- S. viarum.Key points• Biotic elicitors stimulated the alkaloids/glycoalkaloid content in S. viarum plant cultures.• T. reesei was found to be most efficient for enhancing the growth and alkaloids content.• Elicited plants activate ROS based-defense mechanism to overcome oxidative damage. More... »

PAGES

6455-6469

References to SciGraph publications

  • 2013-06-04. Chitosan and a fungal elicitor inhibit tracheary element differentiation and promote accumulation of stress lignin-like substance in Zinnia elegans xylogenic culture in JOURNAL OF PLANT RESEARCH
  • 2018-08-18. Elicitation Enhanced the Yield of Glycyrrhizin and Antioxidant Activities in Hairy Root Cultures of Glycyrrhiza glabra L. in JOURNAL OF PLANT GROWTH REGULATION
  • 2013-06-08. In vitro Production of Secondary Metabolites Using Elicitor in Catharanthus roseus: A Case Study in CROP IMPROVEMENT
  • 2005-06. Induction of Heat Stress Tolerance in Barley Seedlings by Pre-Sowing Seed Treatment with Glycinebetaine in PLANT GROWTH REGULATION
  • 2017-12-29. Transcriptome analysis reveals key roles of AtLBR-2 in LPS-induced defense responses in plants in BMC GENOMICS
  • 2020-07-13. Assessment of direct shoot organogenesis and genetic fidelity in Solanum viarum Dunal—a commercially important medicinal plant in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY - PLANT
  • 2016-01-21. Elicitors’ influenced differential ginsenoside production and exudation into medium with concurrent Rg3/Rh2 panaxadiol induction in Panax quinquefolius cell suspensions in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2012-11-21. Fungal elicitor-mediated enhancement in growth and asiaticoside content of Centella asiatica L. shoot cultures in PLANT GROWTH REGULATION
  • 2019-12-09. Growth and alkaloid production along with expression profiles of biosynthetic pathway genes in two contrasting morphotypes of prickly and prickleless Solanum viarum Dunal in PROTOPLASMA
  • 2018-02-15. Enhanced expression of ginsenoside biosynthetic genes and in vitro ginsenoside production in elicited Panax sikkimensis (Ban) cell suspensions in PROTOPLASMA
  • 2013-06-27. Improved gymnemic acid production in the suspension cultures of Gymnema sylvestre through biotic elicitation in PLANT BIOTECHNOLOGY REPORTS
  • 2020-09-23. Elicitation Improves the Leaf Area, Enzymatic Activities, Antioxidant Activity and Content of Secondary Metabolites in Achillea millefolium L. Grown in the Field in JOURNAL OF PLANT GROWTH REGULATION
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00253-022-12159-8

    DOI

    http://dx.doi.org/10.1007/s00253-022-12159-8

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1150827029

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/36069926


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    28 schema:description Solanum viarum serves as a raw material for the steroidal drug industry due to its alkaloid and glycoalkaloid content. Elicitation is well-known for measuring the increase in the yield of bioactive compounds in in vitro cultures. The current study was performed for the accumulation of metabolites viz. solasodine, solanidine, and α-solanine in S. viarum culture using microbial-based elicitors added in 1%, 3%, 5%, and 7% on 25th and 35th day of culture period and harvested on 45th and 50th days of culture cycle. The treatment of 3% Trichoderma reesei and Bacillus tequilensis culture filtrate (CF) significantly increased biomass, alkaloids/glycoalkaloid content, and yield in S. viarum. T. reesei was found to be the best treatment for enhanced growth (GI = 11.65) and glycoalkaloid yield (2.54 mg DW plant−1) after the 50th day of the culture cycle when added on the 25th day. The abundance of gene transcripts involved in the biosynthesis of alkaloids/glycoalkaloids, revealed by quantitative real-time PCR expression analysis correlates with the accumulation of their respective metabolites in elicited plants. Biochemical analysis shows that elicited plants inhibited oxidative damage caused by reactive oxygen species by activating enzymes (superoxide dismutase and ascorbate peroxidase) as well as non-enzymatic antioxidant mechanisms (alkaloids, total phenols, total flavonoids, carotenoids, and proline). The findings of this study clearly demonstrate that the application of T. reesei and B. tequilensis CF at a specific dose and time significantly improve biomass as well as upregulates the metabolite biosynthetic pathway in an important medicinal plant- S. viarum.Key points• Biotic elicitors stimulated the alkaloids/glycoalkaloid content in S. viarum plant cultures.• T. reesei was found to be most efficient for enhancing the growth and alkaloids content.• Elicited plants activate ROS based-defense mechanism to overcome oxidative damage.
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