Improving extracellular protein production in Escherichia coli by overexpressing D,D-carboxypeptidase to perturb peptidoglycan network synthesis and structure View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2019-01

AUTHORS

Haiquan Yang, Jinyuan Hu, Xiao Lu, Fuxiang Wang, Wei Shen, Wei Hu, Lingling Wang, Xianzhong Chen, Long Liu

ABSTRACT

Most recombinant proteins in Escherichia coli are not efficiently secreted to the extracellular space. Structural stabilisation of the cell wall is essential for extracellular protein production in E. coli, for which D,D-carboxypeptidases are essential. Herein, we perturbed the peptidoglycan structure of the E. coli cell wall by overexpressing D,D-carboxypeptidase genes dacA or dacB, and investigated the effect on extracellular protein production. Overexpression of dacA or dacB promoted the accumulation of intracellular soluble peptidoglycan, altered cell morphology (shape and size) and led to the formation of transparent globular structures in E. coli cells. Compared with controls (CK), extracellular production of recombinant green fluorescent protein (GFP) was increased by 1.7- and 2.3-fold upon overexpression of dacA and dacB, respectively. Similarly, extracellular production of recombinant amylase and α-galactosidase was increased by 4.5- and 2.8-fold, respectively, upon overexpression of dacA, and by 11.9- and 2.5-fold, respectively, upon overexpression of dacB. Overexpression of dacA or dacB enhanced both the outer and inner membrane permeability of E. coli. This cell wall engineering strategy opens up a new direction for enhancing extracellular protein and chemical production in E. coli. More... »

PAGES

1-14

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00253-018-9510-7

DOI

http://dx.doi.org/10.1007/s00253-018-9510-7

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1109811354

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/30417310


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Jiangnan University", 
          "id": "https://www.grid.ac/institutes/grid.258151.a", 
          "name": [
            "The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yang", 
        "givenName": "Haiquan", 
        "id": "sg:person.01071100472.07", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01071100472.07"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Jiangnan University", 
          "id": "https://www.grid.ac/institutes/grid.258151.a", 
          "name": [
            "The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hu", 
        "givenName": "Jinyuan", 
        "id": "sg:person.011276463450.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011276463450.06"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Jiangnan University", 
          "id": "https://www.grid.ac/institutes/grid.258151.a", 
          "name": [
            "The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lu", 
        "givenName": "Xiao", 
        "id": "sg:person.010501103050.86", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010501103050.86"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Jiangnan University", 
          "id": "https://www.grid.ac/institutes/grid.258151.a", 
          "name": [
            "The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wang", 
        "givenName": "Fuxiang", 
        "id": "sg:person.013500570623.33", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013500570623.33"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Jiangnan University", 
          "id": "https://www.grid.ac/institutes/grid.258151.a", 
          "name": [
            "The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Shen", 
        "givenName": "Wei", 
        "id": "sg:person.0605760771.88", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0605760771.88"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institute of Modern Physics", 
          "id": "https://www.grid.ac/institutes/grid.450259.f", 
          "name": [
            "Institute of Modern Physics, Chinese Academy of Sciences, 730000, Lanzhou, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hu", 
        "givenName": "Wei", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Jiangnan University", 
          "id": "https://www.grid.ac/institutes/grid.258151.a", 
          "name": [
            "The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wang", 
        "givenName": "Lingling", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Jiangnan University", 
          "id": "https://www.grid.ac/institutes/grid.258151.a", 
          "name": [
            "The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Chen", 
        "givenName": "Xianzhong", 
        "id": "sg:person.01244540106.60", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01244540106.60"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Jiangnan University", 
          "id": "https://www.grid.ac/institutes/grid.258151.a", 
          "name": [
            "The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Liu", 
        "givenName": "Long", 
        "id": "sg:person.0776151774.31", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0776151774.31"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1111/j.1365-2958.2012.08023.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000616606"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10295-016-1773-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000899897", 
          "https://doi.org/10.1007/s10295-016-1773-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.184.13.3630-3639.2002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001376788"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1098/rstb.2015.0031", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001625902"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0304-4165(71)90053-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008785216"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0304-4165(71)90053-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008785216"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-004-1559-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009268886", 
          "https://doi.org/10.1007/s00253-004-1559-9"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.cell.2010.11.038", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009664830"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1432-1033.1975.tb02183.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010669221"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1574-6976.2007.00099.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011150917"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aac.26.4.546", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012666012"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-1759(88)90414-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012954227"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-1759(88)90414-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012954227"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.tim.2005.10.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015627003"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.tim.2005.10.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015627003"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-006-0465-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017464259", 
          "https://doi.org/10.1007/s00253-006-0465-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-006-0465-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017464259", 
          "https://doi.org/10.1007/s00253-006-0465-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-2958.2003.03607.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018526109"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0076-6879(76)45054-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020324494"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.biotechadv.2004.11.003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020388916"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1432-1033.1974.tb03285.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020815332"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.182.6.1714-1721.2000", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020923787"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.tim.2008.04.006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023197214"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1039/a804532a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023238484"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mmbr.00027-07", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025505427"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0003-2697(76)90527-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025529346"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.186.24.8326-8336.2004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027615565"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrmicro2677", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029395438", 
          "https://doi.org/10.1038/nrmicro2677"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1574-6968.2009.01863.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029691116"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s12257-011-0601-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030125752", 
          "https://doi.org/10.1007/s12257-011-0601-x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0966-842x(94)90127-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030238630"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0966-842x(94)90127-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030238630"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ymben.2012.06.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031194077"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.tim.2009.12.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033614425"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4161/bioe.24761", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034518372"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0006-3495(00)76726-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035417084"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0168-1656(02)00032-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037162109"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1574-6976.2008.00104.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037640548"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s13036-016-0035-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038196793", 
          "https://doi.org/10.1186/s13036-016-0035-2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/s13036-016-0035-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038196793", 
          "https://doi.org/10.1186/s13036-016-0035-2"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.foodchem.2007.11.034", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039885317"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/gtc.12026", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041899305"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbamcr.2004.02.011", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046857442"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.183.10.3055-3064.2001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047634915"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0805309105", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050501598"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.foodchem.2014.09.038", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053114556"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi051533t", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055200978"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi051533t", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055200978"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi700777x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055207360"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi700777x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055207360"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/ja309036q", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055853469"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.174.14.4811-4819.1992", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062720690"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.178.24.7106-7111.1996", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062725476"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.178.3.768-773.1996", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062725535"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074503853", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074548893", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074554002", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078318083", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1081714186", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082972383", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083238187", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083299353", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083355107", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/oxfordjournals.jbchem.a126476", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1083613165"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2019-01", 
    "datePublishedReg": "2019-01-01", 
    "description": "Most recombinant proteins in Escherichia coli are not efficiently secreted to the extracellular space. Structural stabilisation of the cell wall is essential for extracellular protein production in E. coli, for which D,D-carboxypeptidases are essential. Herein, we perturbed the peptidoglycan structure of the E. coli cell wall by overexpressing D,D-carboxypeptidase genes dacA or dacB, and investigated the effect on extracellular protein production. Overexpression of dacA or dacB promoted the accumulation of intracellular soluble peptidoglycan, altered cell morphology (shape and size) and led to the formation of transparent globular structures in E. coli cells. Compared with controls (CK), extracellular production of recombinant green fluorescent protein (GFP) was increased by 1.7- and 2.3-fold upon overexpression of dacA and dacB, respectively. Similarly, extracellular production of recombinant amylase and \u03b1-galactosidase was increased by 4.5- and 2.8-fold, respectively, upon overexpression of dacA, and by 11.9- and 2.5-fold, respectively, upon overexpression of dacB. Overexpression of dacA or dacB enhanced both the outer and inner membrane permeability of E. coli. This cell wall engineering strategy opens up a new direction for enhancing extracellular protein and chemical production in E. coli.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s00253-018-9510-7", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1083533", 
        "issn": [
          "0175-7598", 
          "1432-0614"
        ], 
        "name": "Applied Microbiology and Biotechnology", 
        "type": "Periodical"
      }
    ], 
    "name": "Improving extracellular protein production in Escherichia coli by overexpressing D,D-carboxypeptidase to perturb peptidoglycan network synthesis and structure", 
    "pagination": "1-14", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "61e54e6396551fbbef204b57b5e73d494bce24b0de0b516df2c564086d6193e1"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "30417310"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "8406612"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00253-018-9510-7"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1109811354"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00253-018-9510-7", 
      "https://app.dimensions.ai/details/publication/pub.1109811354"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T20:11", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8681_00000610.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://link.springer.com/10.1007%2Fs00253-018-9510-7"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00253-018-9510-7'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00253-018-9510-7'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00253-018-9510-7'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00253-018-9510-7'


 

This table displays all metadata directly associated to this object as RDF triples.

285 TRIPLES      21 PREDICATES      83 URIs      19 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00253-018-9510-7 schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author Na5e51fc4631b47aa8ad780eb9cb4f4b3
4 schema:citation sg:pub.10.1007/s00253-004-1559-9
5 sg:pub.10.1007/s00253-006-0465-8
6 sg:pub.10.1007/s10295-016-1773-3
7 sg:pub.10.1007/s12257-011-0601-x
8 sg:pub.10.1038/nrmicro2677
9 sg:pub.10.1186/s13036-016-0035-2
10 https://app.dimensions.ai/details/publication/pub.1074503853
11 https://app.dimensions.ai/details/publication/pub.1074548893
12 https://app.dimensions.ai/details/publication/pub.1074554002
13 https://app.dimensions.ai/details/publication/pub.1078318083
14 https://app.dimensions.ai/details/publication/pub.1081714186
15 https://app.dimensions.ai/details/publication/pub.1082972383
16 https://app.dimensions.ai/details/publication/pub.1083238187
17 https://app.dimensions.ai/details/publication/pub.1083299353
18 https://app.dimensions.ai/details/publication/pub.1083355107
19 https://doi.org/10.1016/0003-2697(76)90527-3
20 https://doi.org/10.1016/0022-1759(88)90414-0
21 https://doi.org/10.1016/0304-4165(71)90053-5
22 https://doi.org/10.1016/0966-842x(94)90127-9
23 https://doi.org/10.1016/j.bbamcr.2004.02.011
24 https://doi.org/10.1016/j.biotechadv.2004.11.003
25 https://doi.org/10.1016/j.cell.2010.11.038
26 https://doi.org/10.1016/j.foodchem.2007.11.034
27 https://doi.org/10.1016/j.foodchem.2014.09.038
28 https://doi.org/10.1016/j.tim.2005.10.001
29 https://doi.org/10.1016/j.tim.2008.04.006
30 https://doi.org/10.1016/j.tim.2009.12.004
31 https://doi.org/10.1016/j.ymben.2012.06.005
32 https://doi.org/10.1016/s0006-3495(00)76726-9
33 https://doi.org/10.1016/s0076-6879(76)45054-1
34 https://doi.org/10.1016/s0168-1656(02)00032-9
35 https://doi.org/10.1021/bi051533t
36 https://doi.org/10.1021/bi700777x
37 https://doi.org/10.1021/ja309036q
38 https://doi.org/10.1039/a804532a
39 https://doi.org/10.1046/j.1365-2958.2003.03607.x
40 https://doi.org/10.1073/pnas.0805309105
41 https://doi.org/10.1093/oxfordjournals.jbchem.a126476
42 https://doi.org/10.1098/rstb.2015.0031
43 https://doi.org/10.1111/gtc.12026
44 https://doi.org/10.1111/j.1365-2958.2012.08023.x
45 https://doi.org/10.1111/j.1432-1033.1974.tb03285.x
46 https://doi.org/10.1111/j.1432-1033.1975.tb02183.x
47 https://doi.org/10.1111/j.1574-6968.2009.01863.x
48 https://doi.org/10.1111/j.1574-6976.2007.00099.x
49 https://doi.org/10.1111/j.1574-6976.2008.00104.x
50 https://doi.org/10.1128/aac.26.4.546
51 https://doi.org/10.1128/jb.174.14.4811-4819.1992
52 https://doi.org/10.1128/jb.178.24.7106-7111.1996
53 https://doi.org/10.1128/jb.178.3.768-773.1996
54 https://doi.org/10.1128/jb.182.6.1714-1721.2000
55 https://doi.org/10.1128/jb.183.10.3055-3064.2001
56 https://doi.org/10.1128/jb.184.13.3630-3639.2002
57 https://doi.org/10.1128/jb.186.24.8326-8336.2004
58 https://doi.org/10.1128/mmbr.00027-07
59 https://doi.org/10.4161/bioe.24761
60 schema:datePublished 2019-01
61 schema:datePublishedReg 2019-01-01
62 schema:description Most recombinant proteins in Escherichia coli are not efficiently secreted to the extracellular space. Structural stabilisation of the cell wall is essential for extracellular protein production in E. coli, for which D,D-carboxypeptidases are essential. Herein, we perturbed the peptidoglycan structure of the E. coli cell wall by overexpressing D,D-carboxypeptidase genes dacA or dacB, and investigated the effect on extracellular protein production. Overexpression of dacA or dacB promoted the accumulation of intracellular soluble peptidoglycan, altered cell morphology (shape and size) and led to the formation of transparent globular structures in E. coli cells. Compared with controls (CK), extracellular production of recombinant green fluorescent protein (GFP) was increased by 1.7- and 2.3-fold upon overexpression of dacA and dacB, respectively. Similarly, extracellular production of recombinant amylase and α-galactosidase was increased by 4.5- and 2.8-fold, respectively, upon overexpression of dacA, and by 11.9- and 2.5-fold, respectively, upon overexpression of dacB. Overexpression of dacA or dacB enhanced both the outer and inner membrane permeability of E. coli. This cell wall engineering strategy opens up a new direction for enhancing extracellular protein and chemical production in E. coli.
63 schema:genre research_article
64 schema:inLanguage en
65 schema:isAccessibleForFree false
66 schema:isPartOf sg:journal.1083533
67 schema:name Improving extracellular protein production in Escherichia coli by overexpressing D,D-carboxypeptidase to perturb peptidoglycan network synthesis and structure
68 schema:pagination 1-14
69 schema:productId N2ee237e28eaf4f76b02484df181f6f7b
70 N30ba0912c5ae4a5792fa81014b2bf92b
71 N84881caabfcd41b89459de5fe267d1d0
72 Na0174dad0eb44500be5a8c711b1cf943
73 Nd70b18b1884943a29cb2aa557c101633
74 schema:sameAs https://app.dimensions.ai/details/publication/pub.1109811354
75 https://doi.org/10.1007/s00253-018-9510-7
76 schema:sdDatePublished 2019-04-10T20:11
77 schema:sdLicense https://scigraph.springernature.com/explorer/license/
78 schema:sdPublisher N018150bf12ca40c2bcb54be5e962a897
79 schema:url https://link.springer.com/10.1007%2Fs00253-018-9510-7
80 sgo:license sg:explorer/license/
81 sgo:sdDataset articles
82 rdf:type schema:ScholarlyArticle
83 N018150bf12ca40c2bcb54be5e962a897 schema:name Springer Nature - SN SciGraph project
84 rdf:type schema:Organization
85 N0544d7bb6d1c4f849e65b6e4ce3795b9 rdf:first N349091e733c94f09a83bfb4019df69bf
86 rdf:rest N9896bd01c7d34b0f98195259235d5956
87 N2b7b9b9d43c4440abd61eb46dad09c53 rdf:first sg:person.0776151774.31
88 rdf:rest rdf:nil
89 N2ee237e28eaf4f76b02484df181f6f7b schema:name nlm_unique_id
90 schema:value 8406612
91 rdf:type schema:PropertyValue
92 N30ba0912c5ae4a5792fa81014b2bf92b schema:name doi
93 schema:value 10.1007/s00253-018-9510-7
94 rdf:type schema:PropertyValue
95 N349091e733c94f09a83bfb4019df69bf schema:affiliation https://www.grid.ac/institutes/grid.258151.a
96 schema:familyName Wang
97 schema:givenName Lingling
98 rdf:type schema:Person
99 N625980ba564c4c0eac88bb06e2850abf rdf:first sg:person.0605760771.88
100 rdf:rest Nd27b292afe524aefb9332e67ea1c60d0
101 N7d3ba2ff708945ae8c1735748e76517b rdf:first sg:person.013500570623.33
102 rdf:rest N625980ba564c4c0eac88bb06e2850abf
103 N84881caabfcd41b89459de5fe267d1d0 schema:name dimensions_id
104 schema:value pub.1109811354
105 rdf:type schema:PropertyValue
106 N9896bd01c7d34b0f98195259235d5956 rdf:first sg:person.01244540106.60
107 rdf:rest N2b7b9b9d43c4440abd61eb46dad09c53
108 Na0174dad0eb44500be5a8c711b1cf943 schema:name readcube_id
109 schema:value 61e54e6396551fbbef204b57b5e73d494bce24b0de0b516df2c564086d6193e1
110 rdf:type schema:PropertyValue
111 Na5e51fc4631b47aa8ad780eb9cb4f4b3 rdf:first sg:person.01071100472.07
112 rdf:rest Nc4f220c4dc554abcb8fd5dd2c1baaf66
113 Nc4f220c4dc554abcb8fd5dd2c1baaf66 rdf:first sg:person.011276463450.06
114 rdf:rest Nd1f30fe687fc48359307dfde53996a0d
115 Nd1f30fe687fc48359307dfde53996a0d rdf:first sg:person.010501103050.86
116 rdf:rest N7d3ba2ff708945ae8c1735748e76517b
117 Nd27b292afe524aefb9332e67ea1c60d0 rdf:first Nf64ea12545b8428e83f70130f69dcd8b
118 rdf:rest N0544d7bb6d1c4f849e65b6e4ce3795b9
119 Nd70b18b1884943a29cb2aa557c101633 schema:name pubmed_id
120 schema:value 30417310
121 rdf:type schema:PropertyValue
122 Nf64ea12545b8428e83f70130f69dcd8b schema:affiliation https://www.grid.ac/institutes/grid.450259.f
123 schema:familyName Hu
124 schema:givenName Wei
125 rdf:type schema:Person
126 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
127 schema:name Biological Sciences
128 rdf:type schema:DefinedTerm
129 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
130 schema:name Biochemistry and Cell Biology
131 rdf:type schema:DefinedTerm
132 sg:journal.1083533 schema:issn 0175-7598
133 1432-0614
134 schema:name Applied Microbiology and Biotechnology
135 rdf:type schema:Periodical
136 sg:person.010501103050.86 schema:affiliation https://www.grid.ac/institutes/grid.258151.a
137 schema:familyName Lu
138 schema:givenName Xiao
139 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010501103050.86
140 rdf:type schema:Person
141 sg:person.01071100472.07 schema:affiliation https://www.grid.ac/institutes/grid.258151.a
142 schema:familyName Yang
143 schema:givenName Haiquan
144 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01071100472.07
145 rdf:type schema:Person
146 sg:person.011276463450.06 schema:affiliation https://www.grid.ac/institutes/grid.258151.a
147 schema:familyName Hu
148 schema:givenName Jinyuan
149 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011276463450.06
150 rdf:type schema:Person
151 sg:person.01244540106.60 schema:affiliation https://www.grid.ac/institutes/grid.258151.a
152 schema:familyName Chen
153 schema:givenName Xianzhong
154 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01244540106.60
155 rdf:type schema:Person
156 sg:person.013500570623.33 schema:affiliation https://www.grid.ac/institutes/grid.258151.a
157 schema:familyName Wang
158 schema:givenName Fuxiang
159 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013500570623.33
160 rdf:type schema:Person
161 sg:person.0605760771.88 schema:affiliation https://www.grid.ac/institutes/grid.258151.a
162 schema:familyName Shen
163 schema:givenName Wei
164 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0605760771.88
165 rdf:type schema:Person
166 sg:person.0776151774.31 schema:affiliation https://www.grid.ac/institutes/grid.258151.a
167 schema:familyName Liu
168 schema:givenName Long
169 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0776151774.31
170 rdf:type schema:Person
171 sg:pub.10.1007/s00253-004-1559-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009268886
172 https://doi.org/10.1007/s00253-004-1559-9
173 rdf:type schema:CreativeWork
174 sg:pub.10.1007/s00253-006-0465-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017464259
175 https://doi.org/10.1007/s00253-006-0465-8
176 rdf:type schema:CreativeWork
177 sg:pub.10.1007/s10295-016-1773-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000899897
178 https://doi.org/10.1007/s10295-016-1773-3
179 rdf:type schema:CreativeWork
180 sg:pub.10.1007/s12257-011-0601-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1030125752
181 https://doi.org/10.1007/s12257-011-0601-x
182 rdf:type schema:CreativeWork
183 sg:pub.10.1038/nrmicro2677 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029395438
184 https://doi.org/10.1038/nrmicro2677
185 rdf:type schema:CreativeWork
186 sg:pub.10.1186/s13036-016-0035-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038196793
187 https://doi.org/10.1186/s13036-016-0035-2
188 rdf:type schema:CreativeWork
189 https://app.dimensions.ai/details/publication/pub.1074503853 schema:CreativeWork
190 https://app.dimensions.ai/details/publication/pub.1074548893 schema:CreativeWork
191 https://app.dimensions.ai/details/publication/pub.1074554002 schema:CreativeWork
192 https://app.dimensions.ai/details/publication/pub.1078318083 schema:CreativeWork
193 https://app.dimensions.ai/details/publication/pub.1081714186 schema:CreativeWork
194 https://app.dimensions.ai/details/publication/pub.1082972383 schema:CreativeWork
195 https://app.dimensions.ai/details/publication/pub.1083238187 schema:CreativeWork
196 https://app.dimensions.ai/details/publication/pub.1083299353 schema:CreativeWork
197 https://app.dimensions.ai/details/publication/pub.1083355107 schema:CreativeWork
198 https://doi.org/10.1016/0003-2697(76)90527-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025529346
199 rdf:type schema:CreativeWork
200 https://doi.org/10.1016/0022-1759(88)90414-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012954227
201 rdf:type schema:CreativeWork
202 https://doi.org/10.1016/0304-4165(71)90053-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008785216
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1016/0966-842x(94)90127-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030238630
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1016/j.bbamcr.2004.02.011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046857442
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1016/j.biotechadv.2004.11.003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020388916
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1016/j.cell.2010.11.038 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009664830
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1016/j.foodchem.2007.11.034 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039885317
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1016/j.foodchem.2014.09.038 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053114556
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1016/j.tim.2005.10.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015627003
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1016/j.tim.2008.04.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023197214
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1016/j.tim.2009.12.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033614425
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1016/j.ymben.2012.06.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031194077
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1016/s0006-3495(00)76726-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035417084
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1016/s0076-6879(76)45054-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020324494
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1016/s0168-1656(02)00032-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037162109
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1021/bi051533t schema:sameAs https://app.dimensions.ai/details/publication/pub.1055200978
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1021/bi700777x schema:sameAs https://app.dimensions.ai/details/publication/pub.1055207360
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1021/ja309036q schema:sameAs https://app.dimensions.ai/details/publication/pub.1055853469
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1039/a804532a schema:sameAs https://app.dimensions.ai/details/publication/pub.1023238484
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1046/j.1365-2958.2003.03607.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1018526109
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1073/pnas.0805309105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050501598
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1093/oxfordjournals.jbchem.a126476 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083613165
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1098/rstb.2015.0031 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001625902
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1111/gtc.12026 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041899305
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1111/j.1365-2958.2012.08023.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1000616606
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1111/j.1432-1033.1974.tb03285.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1020815332
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1111/j.1432-1033.1975.tb02183.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1010669221
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1111/j.1574-6968.2009.01863.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1029691116
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1111/j.1574-6976.2007.00099.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1011150917
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1111/j.1574-6976.2008.00104.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1037640548
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1128/aac.26.4.546 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012666012
261 rdf:type schema:CreativeWork
262 https://doi.org/10.1128/jb.174.14.4811-4819.1992 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062720690
263 rdf:type schema:CreativeWork
264 https://doi.org/10.1128/jb.178.24.7106-7111.1996 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062725476
265 rdf:type schema:CreativeWork
266 https://doi.org/10.1128/jb.178.3.768-773.1996 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062725535
267 rdf:type schema:CreativeWork
268 https://doi.org/10.1128/jb.182.6.1714-1721.2000 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020923787
269 rdf:type schema:CreativeWork
270 https://doi.org/10.1128/jb.183.10.3055-3064.2001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047634915
271 rdf:type schema:CreativeWork
272 https://doi.org/10.1128/jb.184.13.3630-3639.2002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001376788
273 rdf:type schema:CreativeWork
274 https://doi.org/10.1128/jb.186.24.8326-8336.2004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027615565
275 rdf:type schema:CreativeWork
276 https://doi.org/10.1128/mmbr.00027-07 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025505427
277 rdf:type schema:CreativeWork
278 https://doi.org/10.4161/bioe.24761 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034518372
279 rdf:type schema:CreativeWork
280 https://www.grid.ac/institutes/grid.258151.a schema:alternateName Jiangnan University
281 schema:name The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, 214122, Wuxi, China
282 rdf:type schema:Organization
283 https://www.grid.ac/institutes/grid.450259.f schema:alternateName Institute of Modern Physics
284 schema:name Institute of Modern Physics, Chinese Academy of Sciences, 730000, Lanzhou, China
285 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...