Impact on strain growth and butenyl-spinosyn biosynthesis by overexpression of polynucleotide phosphorylase gene in Saccharopolyspora pogona View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2018-09

AUTHORS

Li Li, Jie Rang, Haochen He, Siying He, Zhudong Liu, Jianli Tang, Jie Xiao, Lian He, Shengbiao Hu, Ziquan Yu, Xuezhi Ding, Liqiu Xia

ABSTRACT

Polynucleotide phosphorylase is a highly conserved protein found in bacteria and fungi that can regulate the transcription of related enzymes involved in amino acid metabolism, organic acid metabolism, and cell biosynthesis. We studied the effect of polynucleotide phosphorylase on Saccharopolyspora pogona (S. pogona) growth and the synthesis of secondary metabolites. First, we generated the overexpression vector pOJ260-PermE-pnp via overlap extension PCR. The vector pOJ260-PermE-pnp was then introduced into S. pogona by conjugal transfer, thereby generating the recombination strain S. pogona-Pnp. Results showed that engineering strains possessed higher biomass than those of the wild-type strains. Moreover, the ability of these strains to produce spores on solid medium was stronger than that of the wild-type strains. HPLC results revealed that the butenyl-spinosyn yield in S. pogona-Pnp increased by 1.92-fold compared with that of S. pogona alone. These findings revealed that overexpression of polynucleotide phosphorylase effectively promoted butenyl-spinosyn biosynthesis in S. pogona. This result may be extended to other Streptomyces for strain improvement. More... »

PAGES

8011-8021

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00253-018-9178-z

DOI

http://dx.doi.org/10.1007/s00253-018-9178-z

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1105413525

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/29984395


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Bacterial Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Macrolides", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Metabolic Engineering", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Polyribonucleotide Nucleotidyltransferase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Saccharopolyspora", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Hunan Normal University", 
          "id": "https://www.grid.ac/institutes/grid.411427.5", 
          "name": [
            "Hunan Provincial Key Laboratory for Microbial Molecular Biology, State Key Laboratory of Development Biology of Freshwater Fish, College of Life Science, Hunan Normal University, 410081, Changsha, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Li", 
        "givenName": "Li", 
        "id": "sg:person.0747003423.59", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0747003423.59"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hunan Normal University", 
          "id": "https://www.grid.ac/institutes/grid.411427.5", 
          "name": [
            "Hunan Provincial Key Laboratory for Microbial Molecular Biology, State Key Laboratory of Development Biology of Freshwater Fish, College of Life Science, Hunan Normal University, 410081, Changsha, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Rang", 
        "givenName": "Jie", 
        "id": "sg:person.01125660676.03", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01125660676.03"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hunan Normal University", 
          "id": "https://www.grid.ac/institutes/grid.411427.5", 
          "name": [
            "Hunan Provincial Key Laboratory for Microbial Molecular Biology, State Key Laboratory of Development Biology of Freshwater Fish, College of Life Science, Hunan Normal University, 410081, Changsha, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "He", 
        "givenName": "Haochen", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hunan Normal University", 
          "id": "https://www.grid.ac/institutes/grid.411427.5", 
          "name": [
            "Hunan Provincial Key Laboratory for Microbial Molecular Biology, State Key Laboratory of Development Biology of Freshwater Fish, College of Life Science, Hunan Normal University, 410081, Changsha, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "He", 
        "givenName": "Siying", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hunan Normal University", 
          "id": "https://www.grid.ac/institutes/grid.411427.5", 
          "name": [
            "Hunan Provincial Key Laboratory for Microbial Molecular Biology, State Key Laboratory of Development Biology of Freshwater Fish, College of Life Science, Hunan Normal University, 410081, Changsha, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Liu", 
        "givenName": "Zhudong", 
        "id": "sg:person.012616641531.18", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012616641531.18"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hunan Normal University", 
          "id": "https://www.grid.ac/institutes/grid.411427.5", 
          "name": [
            "Hunan Provincial Key Laboratory for Microbial Molecular Biology, State Key Laboratory of Development Biology of Freshwater Fish, College of Life Science, Hunan Normal University, 410081, Changsha, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Tang", 
        "givenName": "Jianli", 
        "id": "sg:person.010426320131.74", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010426320131.74"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hunan Normal University", 
          "id": "https://www.grid.ac/institutes/grid.411427.5", 
          "name": [
            "Hunan Provincial Key Laboratory for Microbial Molecular Biology, State Key Laboratory of Development Biology of Freshwater Fish, College of Life Science, Hunan Normal University, 410081, Changsha, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Xiao", 
        "givenName": "Jie", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hunan Normal University", 
          "id": "https://www.grid.ac/institutes/grid.411427.5", 
          "name": [
            "Hunan Provincial Key Laboratory for Microbial Molecular Biology, State Key Laboratory of Development Biology of Freshwater Fish, College of Life Science, Hunan Normal University, 410081, Changsha, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "He", 
        "givenName": "Lian", 
        "id": "sg:person.01345515642.83", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01345515642.83"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hunan Normal University", 
          "id": "https://www.grid.ac/institutes/grid.411427.5", 
          "name": [
            "Hunan Provincial Key Laboratory for Microbial Molecular Biology, State Key Laboratory of Development Biology of Freshwater Fish, College of Life Science, Hunan Normal University, 410081, Changsha, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hu", 
        "givenName": "Shengbiao", 
        "id": "sg:person.01241103754.05", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01241103754.05"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hunan Normal University", 
          "id": "https://www.grid.ac/institutes/grid.411427.5", 
          "name": [
            "Hunan Provincial Key Laboratory for Microbial Molecular Biology, State Key Laboratory of Development Biology of Freshwater Fish, College of Life Science, Hunan Normal University, 410081, Changsha, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yu", 
        "givenName": "Ziquan", 
        "id": "sg:person.01115040642.62", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01115040642.62"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hunan Normal University", 
          "id": "https://www.grid.ac/institutes/grid.411427.5", 
          "name": [
            "Hunan Provincial Key Laboratory for Microbial Molecular Biology, State Key Laboratory of Development Biology of Freshwater Fish, College of Life Science, Hunan Normal University, 410081, Changsha, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ding", 
        "givenName": "Xuezhi", 
        "id": "sg:person.01234456153.65", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01234456153.65"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Hunan Normal University", 
          "id": "https://www.grid.ac/institutes/grid.411427.5", 
          "name": [
            "Hunan Provincial Key Laboratory for Microbial Molecular Biology, State Key Laboratory of Development Biology of Freshwater Fish, College of Life Science, Hunan Normal University, 410081, Changsha, China"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Xia", 
        "givenName": "Liqiu", 
        "id": "sg:person.0724310244.88", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0724310244.88"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1002/wrna.1328", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000017439"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s11427-012-4276-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002211535", 
          "https://doi.org/10.1007/s11427-012-4276-0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkt095", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004492581"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1477-5956-9-40", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005075227", 
          "https://doi.org/10.1186/1477-5956-9-40"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/mcp.m113.033951", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010753781"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bmc.2009.02.035", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012592423"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(92)90627-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014129383"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(92)90627-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014129383"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt1183-784", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016340344", 
          "https://doi.org/10.1038/nbt1183-784"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.pestbp.2011.11.004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021195828"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.00592-08", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021501154"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.14348/molcells.2014.0168", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022468928"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10295-005-0016-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024782457", 
          "https://doi.org/10.1007/s10295-005-0016-9"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10295-005-0016-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024782457", 
          "https://doi.org/10.1007/s10295-005-0016-9"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.00105-15", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026370641"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-015-6883-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026547007", 
          "https://doi.org/10.1007/s00253-015-6883-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-008-1784-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027652539", 
          "https://doi.org/10.1007/s00253-008-1784-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-008-1784-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027652539", 
          "https://doi.org/10.1007/s00253-008-1784-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/29.9.e45", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030834024"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.185.24.7273-7278.2003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031579978"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/ps.358", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032878640"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aem.00424-12", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033283061"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m110.200741", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034027855"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1477-5956-9-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039827543", 
          "https://doi.org/10.1186/1477-5956-9-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1475-2859-12-26", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041510155", 
          "https://doi.org/10.1186/1475-2859-12-26"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1475-2859-13-27", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042764211", 
          "https://doi.org/10.1186/1475-2859-13-27"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0022-2836(83)80284-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046218244"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1099/mic.0.28363-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060397589"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.169.12.5715-5720.1987", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062715664"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2987/13-6369.1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1070954596"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.00840-16", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1084221946"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1159/000477543", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1091242542"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1103753724", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2018-09", 
    "datePublishedReg": "2018-09-01", 
    "description": "Polynucleotide phosphorylase is a highly conserved protein found in bacteria and fungi that can regulate the transcription of related enzymes involved in amino acid metabolism, organic acid metabolism, and cell biosynthesis. We studied the effect of polynucleotide phosphorylase on Saccharopolyspora pogona (S. pogona) growth and the synthesis of secondary metabolites. First, we generated the overexpression vector pOJ260-PermE-pnp via overlap extension PCR. The vector pOJ260-PermE-pnp was then introduced into S. pogona by conjugal transfer, thereby generating the recombination strain S. pogona-Pnp. Results showed that engineering strains possessed higher biomass than those of the wild-type strains. Moreover, the ability of these strains to produce spores on solid medium was stronger than that of the wild-type strains. HPLC results revealed that the butenyl-spinosyn yield in S. pogona-Pnp increased by 1.92-fold compared with that of S. pogona alone. These findings revealed that overexpression of polynucleotide phosphorylase effectively promoted butenyl-spinosyn biosynthesis in S. pogona. This result may be extended to other Streptomyces for strain improvement.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s00253-018-9178-z", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1083533", 
        "issn": [
          "0175-7598", 
          "1432-0614"
        ], 
        "name": "Applied Microbiology and Biotechnology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "18", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "102"
      }
    ], 
    "name": "Impact on strain growth and butenyl-spinosyn biosynthesis by overexpression of polynucleotide phosphorylase gene in Saccharopolyspora pogona", 
    "pagination": "8011-8021", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "01a6ca25c0a79f07bdbec4ed17c9c901145ea4903052408256ebe9708d871c1c"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "29984395"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "8406612"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00253-018-9178-z"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1105413525"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00253-018-9178-z", 
      "https://app.dimensions.ai/details/publication/pub.1105413525"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T23:40", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8693_00000604.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007%2Fs00253-018-9178-z"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00253-018-9178-z'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00253-018-9178-z'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00253-018-9178-z'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00253-018-9178-z'


 

This table displays all metadata directly associated to this object as RDF triples.

261 TRIPLES      21 PREDICATES      64 URIs      26 LITERALS      14 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00253-018-9178-z schema:about N3341e31587b04cebb6f138b29ac9a26e
2 N4db559b3770d4bcab017760d2637795f
3 N6b067798af1f4431b524badc0250a125
4 Ne94e88493ee146f6bc4d2ccc7b1dc144
5 Nf35eb38578194f4b90cb7b6ac9f8e685
6 anzsrc-for:06
7 anzsrc-for:0601
8 schema:author N7c18c8ee05a64f19bcfa04e8ed60599b
9 schema:citation sg:pub.10.1007/s00253-008-1784-8
10 sg:pub.10.1007/s00253-015-6883-8
11 sg:pub.10.1007/s10295-005-0016-9
12 sg:pub.10.1007/s11427-012-4276-0
13 sg:pub.10.1038/nbt1183-784
14 sg:pub.10.1186/1475-2859-12-26
15 sg:pub.10.1186/1475-2859-13-27
16 sg:pub.10.1186/1477-5956-9-1
17 sg:pub.10.1186/1477-5956-9-40
18 https://app.dimensions.ai/details/publication/pub.1103753724
19 https://doi.org/10.1002/ps.358
20 https://doi.org/10.1002/wrna.1328
21 https://doi.org/10.1016/0378-1119(92)90627-2
22 https://doi.org/10.1016/j.bmc.2009.02.035
23 https://doi.org/10.1016/j.pestbp.2011.11.004
24 https://doi.org/10.1016/s0022-2836(83)80284-8
25 https://doi.org/10.1074/jbc.m110.200741
26 https://doi.org/10.1074/mcp.m113.033951
27 https://doi.org/10.1093/nar/29.9.e45
28 https://doi.org/10.1093/nar/gkt095
29 https://doi.org/10.1099/mic.0.28363-0
30 https://doi.org/10.1128/aem.00424-12
31 https://doi.org/10.1128/jb.00105-15
32 https://doi.org/10.1128/jb.00592-08
33 https://doi.org/10.1128/jb.00840-16
34 https://doi.org/10.1128/jb.169.12.5715-5720.1987
35 https://doi.org/10.1128/jb.185.24.7273-7278.2003
36 https://doi.org/10.1159/000477543
37 https://doi.org/10.14348/molcells.2014.0168
38 https://doi.org/10.2987/13-6369.1
39 schema:datePublished 2018-09
40 schema:datePublishedReg 2018-09-01
41 schema:description Polynucleotide phosphorylase is a highly conserved protein found in bacteria and fungi that can regulate the transcription of related enzymes involved in amino acid metabolism, organic acid metabolism, and cell biosynthesis. We studied the effect of polynucleotide phosphorylase on Saccharopolyspora pogona (S. pogona) growth and the synthesis of secondary metabolites. First, we generated the overexpression vector pOJ260-P<sub>ermE</sub>-pnp via overlap extension PCR. The vector pOJ260-P<sub>ermE</sub>-pnp was then introduced into S. pogona by conjugal transfer, thereby generating the recombination strain S. pogona-Pnp. Results showed that engineering strains possessed higher biomass than those of the wild-type strains. Moreover, the ability of these strains to produce spores on solid medium was stronger than that of the wild-type strains. HPLC results revealed that the butenyl-spinosyn yield in S. pogona-Pnp increased by 1.92-fold compared with that of S. pogona alone. These findings revealed that overexpression of polynucleotide phosphorylase effectively promoted butenyl-spinosyn biosynthesis in S. pogona. This result may be extended to other Streptomyces for strain improvement.
42 schema:genre research_article
43 schema:inLanguage en
44 schema:isAccessibleForFree false
45 schema:isPartOf N24c6a3c9ff6748ca8432b5ec40cbbfb4
46 N4c12c65ab9994cdb9643a171693dabfa
47 sg:journal.1083533
48 schema:name Impact on strain growth and butenyl-spinosyn biosynthesis by overexpression of polynucleotide phosphorylase gene in Saccharopolyspora pogona
49 schema:pagination 8011-8021
50 schema:productId N2d19654972134adfb8e34d0c813d32ba
51 N2f5d072e7d914c9e84ceef3739e0c9a5
52 N3d697327020b43e5b22adf07b2100fe4
53 N57cd135faf134e069cd6e3319b973c90
54 Ncec3c5670b8e4ab4a4ed8fa06773f1ee
55 schema:sameAs https://app.dimensions.ai/details/publication/pub.1105413525
56 https://doi.org/10.1007/s00253-018-9178-z
57 schema:sdDatePublished 2019-04-10T23:40
58 schema:sdLicense https://scigraph.springernature.com/explorer/license/
59 schema:sdPublisher N36d02c2acbd047789179df2248fa27e4
60 schema:url http://link.springer.com/10.1007%2Fs00253-018-9178-z
61 sgo:license sg:explorer/license/
62 sgo:sdDataset articles
63 rdf:type schema:ScholarlyArticle
64 N070dbe77afe6423f9db5a9192fb87606 rdf:first sg:person.01241103754.05
65 rdf:rest N6149b07c91c24fc381ac149640b09063
66 N17db47b48baf435c94c195264cb9998b rdf:first N83b59985001e4af09a3b3868176f0dcb
67 rdf:rest N5a88a5471e0641c28b826392c5ec4415
68 N24039c3ec4814e07a33719cc23f4de42 rdf:first sg:person.0724310244.88
69 rdf:rest rdf:nil
70 N24c6a3c9ff6748ca8432b5ec40cbbfb4 schema:issueNumber 18
71 rdf:type schema:PublicationIssue
72 N2d19654972134adfb8e34d0c813d32ba schema:name doi
73 schema:value 10.1007/s00253-018-9178-z
74 rdf:type schema:PropertyValue
75 N2f5d072e7d914c9e84ceef3739e0c9a5 schema:name nlm_unique_id
76 schema:value 8406612
77 rdf:type schema:PropertyValue
78 N3341e31587b04cebb6f138b29ac9a26e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
79 schema:name Saccharopolyspora
80 rdf:type schema:DefinedTerm
81 N36d02c2acbd047789179df2248fa27e4 schema:name Springer Nature - SN SciGraph project
82 rdf:type schema:Organization
83 N3d697327020b43e5b22adf07b2100fe4 schema:name readcube_id
84 schema:value 01a6ca25c0a79f07bdbec4ed17c9c901145ea4903052408256ebe9708d871c1c
85 rdf:type schema:PropertyValue
86 N4c12c65ab9994cdb9643a171693dabfa schema:volumeNumber 102
87 rdf:type schema:PublicationVolume
88 N4db559b3770d4bcab017760d2637795f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
89 schema:name Polyribonucleotide Nucleotidyltransferase
90 rdf:type schema:DefinedTerm
91 N57cd135faf134e069cd6e3319b973c90 schema:name dimensions_id
92 schema:value pub.1105413525
93 rdf:type schema:PropertyValue
94 N5a88a5471e0641c28b826392c5ec4415 rdf:first sg:person.01345515642.83
95 rdf:rest N070dbe77afe6423f9db5a9192fb87606
96 N6149b07c91c24fc381ac149640b09063 rdf:first sg:person.01115040642.62
97 rdf:rest Naf49f92854f14f22bb07be2bbbe4bc49
98 N6a2a64e9c2124823a08217f0a253d76c rdf:first sg:person.010426320131.74
99 rdf:rest N17db47b48baf435c94c195264cb9998b
100 N6b067798af1f4431b524badc0250a125 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
101 schema:name Macrolides
102 rdf:type schema:DefinedTerm
103 N7c18c8ee05a64f19bcfa04e8ed60599b rdf:first sg:person.0747003423.59
104 rdf:rest Ne865dcb047c6422caf4aa10115195860
105 N83b59985001e4af09a3b3868176f0dcb schema:affiliation https://www.grid.ac/institutes/grid.411427.5
106 schema:familyName Xiao
107 schema:givenName Jie
108 rdf:type schema:Person
109 N99582c60a0744c87b1822197c5c51fa5 rdf:first Nc824ef6134c34e588fa0aea862544a2f
110 rdf:rest Na81b28a20d37469896e81d7813a9e9cc
111 N9de21b4c91ab4ae18da777eec5123f06 rdf:first sg:person.012616641531.18
112 rdf:rest N6a2a64e9c2124823a08217f0a253d76c
113 Na81b28a20d37469896e81d7813a9e9cc rdf:first Ncfd22bb6b5a84858a4bda9e5a19aefbd
114 rdf:rest N9de21b4c91ab4ae18da777eec5123f06
115 Naf49f92854f14f22bb07be2bbbe4bc49 rdf:first sg:person.01234456153.65
116 rdf:rest N24039c3ec4814e07a33719cc23f4de42
117 Nc824ef6134c34e588fa0aea862544a2f schema:affiliation https://www.grid.ac/institutes/grid.411427.5
118 schema:familyName He
119 schema:givenName Haochen
120 rdf:type schema:Person
121 Ncec3c5670b8e4ab4a4ed8fa06773f1ee schema:name pubmed_id
122 schema:value 29984395
123 rdf:type schema:PropertyValue
124 Ncfd22bb6b5a84858a4bda9e5a19aefbd schema:affiliation https://www.grid.ac/institutes/grid.411427.5
125 schema:familyName He
126 schema:givenName Siying
127 rdf:type schema:Person
128 Ne865dcb047c6422caf4aa10115195860 rdf:first sg:person.01125660676.03
129 rdf:rest N99582c60a0744c87b1822197c5c51fa5
130 Ne94e88493ee146f6bc4d2ccc7b1dc144 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
131 schema:name Metabolic Engineering
132 rdf:type schema:DefinedTerm
133 Nf35eb38578194f4b90cb7b6ac9f8e685 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
134 schema:name Bacterial Proteins
135 rdf:type schema:DefinedTerm
136 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
137 schema:name Biological Sciences
138 rdf:type schema:DefinedTerm
139 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
140 schema:name Biochemistry and Cell Biology
141 rdf:type schema:DefinedTerm
142 sg:journal.1083533 schema:issn 0175-7598
143 1432-0614
144 schema:name Applied Microbiology and Biotechnology
145 rdf:type schema:Periodical
146 sg:person.010426320131.74 schema:affiliation https://www.grid.ac/institutes/grid.411427.5
147 schema:familyName Tang
148 schema:givenName Jianli
149 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010426320131.74
150 rdf:type schema:Person
151 sg:person.01115040642.62 schema:affiliation https://www.grid.ac/institutes/grid.411427.5
152 schema:familyName Yu
153 schema:givenName Ziquan
154 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01115040642.62
155 rdf:type schema:Person
156 sg:person.01125660676.03 schema:affiliation https://www.grid.ac/institutes/grid.411427.5
157 schema:familyName Rang
158 schema:givenName Jie
159 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01125660676.03
160 rdf:type schema:Person
161 sg:person.01234456153.65 schema:affiliation https://www.grid.ac/institutes/grid.411427.5
162 schema:familyName Ding
163 schema:givenName Xuezhi
164 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01234456153.65
165 rdf:type schema:Person
166 sg:person.01241103754.05 schema:affiliation https://www.grid.ac/institutes/grid.411427.5
167 schema:familyName Hu
168 schema:givenName Shengbiao
169 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01241103754.05
170 rdf:type schema:Person
171 sg:person.012616641531.18 schema:affiliation https://www.grid.ac/institutes/grid.411427.5
172 schema:familyName Liu
173 schema:givenName Zhudong
174 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012616641531.18
175 rdf:type schema:Person
176 sg:person.01345515642.83 schema:affiliation https://www.grid.ac/institutes/grid.411427.5
177 schema:familyName He
178 schema:givenName Lian
179 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01345515642.83
180 rdf:type schema:Person
181 sg:person.0724310244.88 schema:affiliation https://www.grid.ac/institutes/grid.411427.5
182 schema:familyName Xia
183 schema:givenName Liqiu
184 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0724310244.88
185 rdf:type schema:Person
186 sg:person.0747003423.59 schema:affiliation https://www.grid.ac/institutes/grid.411427.5
187 schema:familyName Li
188 schema:givenName Li
189 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0747003423.59
190 rdf:type schema:Person
191 sg:pub.10.1007/s00253-008-1784-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027652539
192 https://doi.org/10.1007/s00253-008-1784-8
193 rdf:type schema:CreativeWork
194 sg:pub.10.1007/s00253-015-6883-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026547007
195 https://doi.org/10.1007/s00253-015-6883-8
196 rdf:type schema:CreativeWork
197 sg:pub.10.1007/s10295-005-0016-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024782457
198 https://doi.org/10.1007/s10295-005-0016-9
199 rdf:type schema:CreativeWork
200 sg:pub.10.1007/s11427-012-4276-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002211535
201 https://doi.org/10.1007/s11427-012-4276-0
202 rdf:type schema:CreativeWork
203 sg:pub.10.1038/nbt1183-784 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016340344
204 https://doi.org/10.1038/nbt1183-784
205 rdf:type schema:CreativeWork
206 sg:pub.10.1186/1475-2859-12-26 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041510155
207 https://doi.org/10.1186/1475-2859-12-26
208 rdf:type schema:CreativeWork
209 sg:pub.10.1186/1475-2859-13-27 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042764211
210 https://doi.org/10.1186/1475-2859-13-27
211 rdf:type schema:CreativeWork
212 sg:pub.10.1186/1477-5956-9-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039827543
213 https://doi.org/10.1186/1477-5956-9-1
214 rdf:type schema:CreativeWork
215 sg:pub.10.1186/1477-5956-9-40 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005075227
216 https://doi.org/10.1186/1477-5956-9-40
217 rdf:type schema:CreativeWork
218 https://app.dimensions.ai/details/publication/pub.1103753724 schema:CreativeWork
219 https://doi.org/10.1002/ps.358 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032878640
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1002/wrna.1328 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000017439
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1016/0378-1119(92)90627-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014129383
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1016/j.bmc.2009.02.035 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012592423
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1016/j.pestbp.2011.11.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021195828
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1016/s0022-2836(83)80284-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046218244
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1074/jbc.m110.200741 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034027855
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1074/mcp.m113.033951 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010753781
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1093/nar/29.9.e45 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030834024
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1093/nar/gkt095 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004492581
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1099/mic.0.28363-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060397589
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1128/aem.00424-12 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033283061
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1128/jb.00105-15 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026370641
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1128/jb.00592-08 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021501154
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1128/jb.00840-16 schema:sameAs https://app.dimensions.ai/details/publication/pub.1084221946
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1128/jb.169.12.5715-5720.1987 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062715664
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1128/jb.185.24.7273-7278.2003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031579978
252 rdf:type schema:CreativeWork
253 https://doi.org/10.1159/000477543 schema:sameAs https://app.dimensions.ai/details/publication/pub.1091242542
254 rdf:type schema:CreativeWork
255 https://doi.org/10.14348/molcells.2014.0168 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022468928
256 rdf:type schema:CreativeWork
257 https://doi.org/10.2987/13-6369.1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1070954596
258 rdf:type schema:CreativeWork
259 https://www.grid.ac/institutes/grid.411427.5 schema:alternateName Hunan Normal University
260 schema:name Hunan Provincial Key Laboratory for Microbial Molecular Biology, State Key Laboratory of Development Biology of Freshwater Fish, College of Life Science, Hunan Normal University, 410081, Changsha, China
261 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...