Ontology type: schema:ScholarlyArticle
2018-07
AUTHORSFitsum Tigu, Junli Zhang, Guoxia Liu, Zhen Cai, Yin Li
ABSTRACTD-Pantothenic acid (vitamin B5) has wide applications in the feed, food, chemical, and pharmaceutical industries. Its biological production routes which employ pantothenate synthetase (PS) as the key enzyme are attractive since they avoid the tedious and time-consuming optical resolution process. However, little data is available on the activity and kinetics of this enzyme, hampering the rational selection of an efficient enzyme for the biological production of D-pantothenic acid. In this study, six phylogenetically distant PS-encoding genes, from Escherichia coli, Corynebacterium glutamicum, Bacillus subtilis, Bacillus thuringiensis, Bacillus cereus, and Enterobacter cloacae, were expressed in E. coli. The PS from C. glutamicum exhibited a specific activity of 205.1 U/mg and a turnover number of 127.6 s-1, which to our best knowledge are the highest values ever reported. The addition of substrates (D-pantoic acid and β-alanine) to the E. coli strain harboring this enzyme during the early log phase of fermentation resulted in the production of 97.1 g/L of D-pantothenic acid within 32 h, corresponding to a conversion yield of 99.1% and a productivity of 3.0 g/L/h. To the best of our knowledge, this is the highest productivity reported to date. More... »
PAGES6039-6046
http://scigraph.springernature.com/pub.10.1007/s00253-018-9017-2
DOIhttp://dx.doi.org/10.1007/s00253-018-9017-2
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1103859252
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/29737393
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Genetics",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Corynebacterium glutamicum",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Escherichia coli",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Fermentation",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Industrial Microbiology",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Pantothenic Acid",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Peptide Synthases",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Recombinant Proteins",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "University of Chinese Academy of Sciences",
"id": "https://www.grid.ac/institutes/grid.410726.6",
"name": [
"CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, No. 1 West Beichen Road, Chaoyang District, 100101, Beijing, China",
"University of Chinese Academy of Sciences, 100190, Beijing, China"
],
"type": "Organization"
},
"familyName": "Tigu",
"givenName": "Fitsum",
"id": "sg:person.010716273327.18",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010716273327.18"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Chinese Academy of Sciences",
"id": "https://www.grid.ac/institutes/grid.410726.6",
"name": [
"CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, No. 1 West Beichen Road, Chaoyang District, 100101, Beijing, China",
"University of Chinese Academy of Sciences, 100190, Beijing, China"
],
"type": "Organization"
},
"familyName": "Zhang",
"givenName": "Junli",
"id": "sg:person.0735441266.68",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0735441266.68"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Institute of Microbiology",
"id": "https://www.grid.ac/institutes/grid.458488.d",
"name": [
"CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, No. 1 West Beichen Road, Chaoyang District, 100101, Beijing, China"
],
"type": "Organization"
},
"familyName": "Liu",
"givenName": "Guoxia",
"id": "sg:person.0667326066.50",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0667326066.50"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Institute of Microbiology",
"id": "https://www.grid.ac/institutes/grid.458488.d",
"name": [
"CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, No. 1 West Beichen Road, Chaoyang District, 100101, Beijing, China"
],
"type": "Organization"
},
"familyName": "Cai",
"givenName": "Zhen",
"id": "sg:person.01107360260.52",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01107360260.52"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Institute of Microbiology",
"id": "https://www.grid.ac/institutes/grid.458488.d",
"name": [
"CAS Key Laboratory of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, No. 1 West Beichen Road, Chaoyang District, 100101, Beijing, China"
],
"type": "Organization"
},
"familyName": "Li",
"givenName": "Yin",
"id": "sg:person.01305454441.89",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01305454441.89"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1007/10_2010_75",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1010358113",
"https://doi.org/10.1007/10_2010_75"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/10_2010_75",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1010358113",
"https://doi.org/10.1007/10_2010_75"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.jpba.2012.06.020",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1011557915"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1039/b316419p",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1022630759"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0168-1656(03)00146-9",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1022913330"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0168-1656(03)00146-9",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1022913330"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf02932910",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1027706611",
"https://doi.org/10.1007/bf02932910"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf02932910",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1027706611",
"https://doi.org/10.1007/bf02932910"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0021-9673(01)93935-4",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1029648000"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1042/bj3410669",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1032046287"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1042/bj3410669",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1032046287"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.3177/jnsv.24.243",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044325000"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.apcata.2004.08.028",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1046762609"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0032-9592(02)00164-4",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1050220650"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0032-9592(02)00164-4",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1050220650"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/bi011522+",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1055194340"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/bi011522+",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1055194340"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/bi1004206",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1055203155"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/bi1004206",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1055203155"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/ie050774u",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1055599229"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/ie050774u",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1055599229"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/ja01221a503",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1055783915"
],
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1074365794",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1075369755",
"type": "CreativeWork"
}
],
"datePublished": "2018-07",
"datePublishedReg": "2018-07-01",
"description": "D-Pantothenic acid (vitamin B5) has wide applications in the feed, food, chemical, and pharmaceutical industries. Its biological production routes which employ pantothenate synthetase (PS) as the key enzyme are attractive since they avoid the tedious and time-consuming optical resolution process. However, little data is available on the activity and kinetics of this enzyme, hampering the rational selection of an efficient enzyme for the biological production of D-pantothenic acid. In this study, six phylogenetically distant PS-encoding genes, from Escherichia coli, Corynebacterium glutamicum, Bacillus subtilis, Bacillus thuringiensis, Bacillus cereus, and Enterobacter cloacae, were expressed in E. coli. The PS from C. glutamicum exhibited a specific activity of 205.1\u00a0U/mg and a turnover number of 127.6\u00a0s-1, which to our best knowledge are the highest values ever reported. The addition of substrates (D-pantoic acid and \u03b2-alanine) to the E. coli strain harboring this enzyme during the early log phase of fermentation resulted in the production of 97.1\u00a0g/L of D-pantothenic acid within 32\u00a0h, corresponding to a conversion yield of 99.1% and a productivity of 3.0\u00a0g/L/h. To the best of our knowledge, this is the highest productivity reported to date.",
"genre": "research_article",
"id": "sg:pub.10.1007/s00253-018-9017-2",
"inLanguage": [
"en"
],
"isAccessibleForFree": false,
"isPartOf": [
{
"id": "sg:journal.1083533",
"issn": [
"0175-7598",
"1432-0614"
],
"name": "Applied Microbiology and Biotechnology",
"type": "Periodical"
},
{
"issueNumber": "14",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "102"
}
],
"name": "A highly active pantothenate synthetase from Corynebacterium glutamicum enables the production of d-pantothenic acid with high productivity",
"pagination": "6039-6046",
"productId": [
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"448c8638f68366a7ffa948b3b80361d79d805f7dad3f1c49645000a556242e05"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"29737393"
]
},
{
"name": "nlm_unique_id",
"type": "PropertyValue",
"value": [
"8406612"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/s00253-018-9017-2"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1103859252"
]
}
],
"sameAs": [
"https://doi.org/10.1007/s00253-018-9017-2",
"https://app.dimensions.ai/details/publication/pub.1103859252"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-10T17:45",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8672_00000604.jsonl",
"type": "ScholarlyArticle",
"url": "http://link.springer.com/10.1007%2Fs00253-018-9017-2"
}
]
Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00253-018-9017-2'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00253-018-9017-2'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00253-018-9017-2'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00253-018-9017-2'
This table displays all metadata directly associated to this object as RDF triples.
177 TRIPLES
21 PREDICATES
52 URIs
28 LITERALS
16 BLANK NODES