Thermostable multifunctional GH74 xyloglucanase from Myceliophthora thermophila: high-level expression in Pichia pastoris and characterization of the recombinant protein View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2017-07

AUTHORS

Oksana V. Berezina, Jonathan Herlet, Sergey V. Rykov, Petra Kornberger, Artem Zavyalov, Dmitriy Kozlov, Liliya Sakhibgaraeva, Irina Krestyanova, Wolfgang H. Schwarz, Vladimir V. Zverlov, Wolfgang Liebl, Sergey V. Yarotsky

ABSTRACT

A xyloglucanase of the GH74 family was identified in the thermophilic fungus strain Myceliophthora thermophila VKPM F-244, and its gene sequence was optimized for cloning and expression in Pichia pastoris. The recombinant xyloglucanase MtXgh74 exhibited the highest activity toward tamarind seed xyloglucan with a K M value of 0.51 ± 0.06 mg/mL. The activities on barley β-glucan and carboxymethylcellulose were about 4 and 2%, respectively, compared to xyloglucan. Maximum xyloglucanase activity was observed at 70-75 °C and pH 6.5. After pre-incubation at 50 °C, pH 6.0 for 3 h, the enzyme retained 100% of its activity. The half-life of MtXgh74 at 60 °C, pH 6.0 was 40 min. In P. pastoris, MtXgh74 was produced in glycosylated form. The enzyme production in a 1 L bioreactor resulted in a yield of 118 U/mL or 5.3 g/L after 51 h fermentation. Kinetic studies of the hydrolysis product formation suggest that MtXgh74 has an endo-processive mode of action. The final products were the standard xyloglucan building blocks XXXG, XXLG, XLXG, and XLLG. Additionally, MtXgh74 hydrolyzed various linkages within the xyloglucan building blocks XXXG, XXLG, and XLXG (except XLLG) producing diverse low molecular weight oligosaccharides which may be identified by MALDI-TOF as XG, XX, XXG/GXX/XGX, XXX, LG, LX/XL, XLX/XXL, LLG, GXXXG, GXLLG, XLLGX. The unique combination of different activities within one enzyme along with its high thermostability and specificity toward xyloglucan makes MtXgh74 a promising candidate enzyme for industrial applications. More... »

PAGES

5653-5666

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00253-017-8297-2

DOI

http://dx.doi.org/10.1007/s00253-017-8297-2

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1085191910

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/28477154


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Carboxymethylcellulose Sodium", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cloning, Molecular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Enzyme Stability", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Glucans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Glycoside Hydrolases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Half-Life", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hydrogen-Ion Concentration", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hydrolysis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Kinetics", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Weight", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Pichia", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Recombinant Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sordariales", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Substrate Specificity", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Temperature", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Xylans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "beta-Glucans", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Genetika", 
          "id": "https://www.grid.ac/institutes/grid.418697.5", 
          "name": [
            "State Research Institute of Genetics and Selection of Industrial Microorganisms, 1-st Dorojniy pr. 1, 117545, Moscow, Russian Federation"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Berezina", 
        "givenName": "Oksana V.", 
        "id": "sg:person.01366311264.41", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01366311264.41"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Technical University Munich", 
          "id": "https://www.grid.ac/institutes/grid.6936.a", 
          "name": [
            "Department of Microbiology, Technical University Munich, Emil-Ramann-Str. 4, 85354, Freising, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Herlet", 
        "givenName": "Jonathan", 
        "id": "sg:person.015051330373.06", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015051330373.06"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Genetika", 
          "id": "https://www.grid.ac/institutes/grid.418697.5", 
          "name": [
            "State Research Institute of Genetics and Selection of Industrial Microorganisms, 1-st Dorojniy pr. 1, 117545, Moscow, Russian Federation"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Rykov", 
        "givenName": "Sergey V.", 
        "id": "sg:person.0653103754.69", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0653103754.69"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Technical University Munich", 
          "id": "https://www.grid.ac/institutes/grid.6936.a", 
          "name": [
            "Department of Microbiology, Technical University Munich, Emil-Ramann-Str. 4, 85354, Freising, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kornberger", 
        "givenName": "Petra", 
        "id": "sg:person.0725145416.81", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0725145416.81"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Genetika", 
          "id": "https://www.grid.ac/institutes/grid.418697.5", 
          "name": [
            "State Research Institute of Genetics and Selection of Industrial Microorganisms, 1-st Dorojniy pr. 1, 117545, Moscow, Russian Federation"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zavyalov", 
        "givenName": "Artem", 
        "id": "sg:person.07741220173.38", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07741220173.38"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Genetika", 
          "id": "https://www.grid.ac/institutes/grid.418697.5", 
          "name": [
            "State Research Institute of Genetics and Selection of Industrial Microorganisms, 1-st Dorojniy pr. 1, 117545, Moscow, Russian Federation"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kozlov", 
        "givenName": "Dmitriy", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Genetika", 
          "id": "https://www.grid.ac/institutes/grid.418697.5", 
          "name": [
            "State Research Institute of Genetics and Selection of Industrial Microorganisms, 1-st Dorojniy pr. 1, 117545, Moscow, Russian Federation"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sakhibgaraeva", 
        "givenName": "Liliya", 
        "id": "sg:person.011334161173.00", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011334161173.00"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Genetika", 
          "id": "https://www.grid.ac/institutes/grid.418697.5", 
          "name": [
            "State Research Institute of Genetics and Selection of Industrial Microorganisms, 1-st Dorojniy pr. 1, 117545, Moscow, Russian Federation"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Krestyanova", 
        "givenName": "Irina", 
        "id": "sg:person.01133641461.15", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01133641461.15"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Technical University Munich", 
          "id": "https://www.grid.ac/institutes/grid.6936.a", 
          "name": [
            "Department of Microbiology, Technical University Munich, Emil-Ramann-Str. 4, 85354, Freising, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Schwarz", 
        "givenName": "Wolfgang H.", 
        "id": "sg:person.011150256722.54", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011150256722.54"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Russian Academy of Sciences", 
          "id": "https://www.grid.ac/institutes/grid.4886.2", 
          "name": [
            "Department of Microbiology, Technical University Munich, Emil-Ramann-Str. 4, 85354, Freising, Germany", 
            "Institute of Molecular Genetics, Russian Academy of Science, Kurchatov Sq. 2, 123182, Moscow, Russian Federation"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zverlov", 
        "givenName": "Vladimir V.", 
        "id": "sg:person.01207074472.65", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01207074472.65"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Technical University Munich", 
          "id": "https://www.grid.ac/institutes/grid.6936.a", 
          "name": [
            "Department of Microbiology, Technical University Munich, Emil-Ramann-Str. 4, 85354, Freising, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Liebl", 
        "givenName": "Wolfgang", 
        "id": "sg:person.0624523277.21", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0624523277.21"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Genetika", 
          "id": "https://www.grid.ac/institutes/grid.418697.5", 
          "name": [
            "State Research Institute of Genetics and Selection of Industrial Microorganisms, 1-st Dorojniy pr. 1, 117545, Moscow, Russian Federation"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yarotsky", 
        "givenName": "Sergey V.", 
        "id": "sg:person.0767160074.28", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0767160074.28"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1002/pmic.201400199", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002186635"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt.1976", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002490916", 
          "https://doi.org/10.1038/nbt.1976"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbagen.2004.07.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006012557"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1574-6976.2000.tb00532.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009711357"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/227680a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010419937", 
          "https://doi.org/10.1038/227680a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/prep.1997.0765", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011661480"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1742-4658.2009.07205.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012506154"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1107/s1744309113030005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012771977"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1385/mb:16:1:23", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013013866", 
          "https://doi.org/10.1385/mb:16:1:23"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0076-6879(88)60109-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013265697"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4314/jasem.v10i3.17326", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013551669"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4314/jasem.v10i3.17326", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013551669"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/bit.260250717", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013563357"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0958-1669(02)00330-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014666008"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0008-6215(96)00325-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014941455"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/ind.2011.7.214", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014971996"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/jmr.687", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015247455"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.biortech.2006.02.009", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017868633"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.str.2004.04.020", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020607291"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/(sici)1097-0061(199908)15:11<1087::aid-yea436>3.0.co;2-f", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025354919"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/(sici)1097-0061(199908)15:11<1087::aid-yea436>3.0.co;2-f", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025354919"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/glycob/cwh036", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026521040"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bp050361o", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027022017"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aem.01922-10", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027430512"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3389/fmicb.2014.00281", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028492795"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.febslet.2014.03.023", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031978948"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10529-008-9739-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032544790", 
          "https://doi.org/10.1007/s10529-008-9739-9"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10529-008-9739-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032544790", 
          "https://doi.org/10.1007/s10529-008-9739-9"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0003-2697(87)90369-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032863794"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10719-007-9082-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032879408", 
          "https://doi.org/10.1007/s10719-007-9082-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1399-3054.1993.tb01778.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034828749"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.femsyr.2004.12.011", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040705677"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aem.01762-12", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040881097"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/bit.10616", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045652318"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1470-8744.1999.tb00770.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046776813"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m208443200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049235227"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0168-1656(03)00003-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050369945"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.carres.2007.10.014", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052506393"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/b:biry.0000029853.34093.13", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053671139", 
          "https://doi.org/10.1023/b:biry.0000029853.34093.13"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.3103/s089141681603006x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053881651", 
          "https://doi.org/10.3103/s089141681603006x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.3103/s089141681603006x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053881651", 
          "https://doi.org/10.3103/s089141681603006x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1099/00221287-136-10-1967", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1060367505"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/1091581813484069", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1063976692"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1177/1091581813484069", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1063976692"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4014/jmb.1109.09031", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1071872177"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077157407", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1083127471", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2017-07", 
    "datePublishedReg": "2017-07-01", 
    "description": "A xyloglucanase of the GH74 family was identified in the thermophilic fungus strain Myceliophthora thermophila VKPM F-244, and its gene sequence was optimized for cloning and expression in Pichia pastoris. The recombinant xyloglucanase MtXgh74 exhibited the highest activity toward tamarind seed xyloglucan with a K M value of 0.51\u00a0\u00b1\u00a00.06\u00a0mg/mL. The activities on barley \u03b2-glucan and carboxymethylcellulose were about 4 and 2%, respectively, compared to xyloglucan. Maximum xyloglucanase activity was observed at 70-75\u00a0\u00b0C and pH\u00a06.5. After pre-incubation at 50\u00a0\u00b0C, pH\u00a06.0 for 3\u00a0h, the enzyme retained 100% of its activity. The half-life of MtXgh74 at 60\u00a0\u00b0C, pH\u00a06.0 was 40\u00a0min. In P. pastoris, MtXgh74 was produced in glycosylated form. The enzyme production in a 1 L bioreactor resulted in a yield of 118\u00a0U/mL or 5.3\u00a0g/L after 51 h fermentation. Kinetic studies of the hydrolysis product formation suggest that MtXgh74 has an endo-processive mode of action. The final products were the standard xyloglucan building blocks XXXG, XXLG, XLXG, and XLLG. Additionally, MtXgh74 hydrolyzed various linkages within the xyloglucan building blocks XXXG, XXLG, and XLXG (except XLLG) producing diverse low molecular weight oligosaccharides which may be identified by MALDI-TOF as XG, XX, XXG/GXX/XGX, XXX, LG, LX/XL, XLX/XXL, LLG, GXXXG, GXLLG, XLLGX. The unique combination of different activities within one enzyme along with its high thermostability and specificity toward xyloglucan makes MtXgh74 a promising candidate enzyme for industrial applications.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s00253-017-8297-2", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1083533", 
        "issn": [
          "0175-7598", 
          "1432-0614"
        ], 
        "name": "Applied Microbiology and Biotechnology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "14", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "101"
      }
    ], 
    "name": "Thermostable multifunctional GH74 xyloglucanase from Myceliophthora thermophila: high-level expression in Pichia pastoris and characterization of the recombinant protein", 
    "pagination": "5653-5666", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "92cd7393cebadc6a213e9c1da379585eab5149802d35c36a63f0618e9d2d3368"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "28477154"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "8406612"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00253-017-8297-2"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1085191910"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00253-017-8297-2", 
      "https://app.dimensions.ai/details/publication/pub.1085191910"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T10:21", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000348_0000000348/records_54336_00000001.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://link.springer.com/10.1007%2Fs00253-017-8297-2"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00253-017-8297-2'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00253-017-8297-2'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00253-017-8297-2'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00253-017-8297-2'


 

This table displays all metadata directly associated to this object as RDF triples.

351 TRIPLES      21 PREDICATES      88 URIs      38 LITERALS      26 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00253-017-8297-2 schema:about N0dc17669ecf443aaa6967a57a7429431
2 N1244aea4b5ee4ecab436b6bd4322c5e1
3 N26b91beb8c224b69b332d612d9c7fa97
4 N431b57431900466c9ed4d79ebf870044
5 N4c9b31f932e74ba98795be6c44b00173
6 N6c792fa7686647e0938b4426793051a4
7 N70e2188b4aa04e6f85cd9f3c6dfe0d31
8 N71abef1fbc424b29a69ce483b11d45d3
9 N738741264f4040d69e00917f8284e66d
10 N8122dc7106be4b239b9e111659819101
11 N9d513f3da77b41a29457aa3aa86ba5aa
12 Na166231e771e49b1a99871d63842a556
13 Nca07813a96be450cb92ff23be6a53705
14 Ncc412f6508ba45ac9fe8b20b9964b2dd
15 Nd042bc1a4c2b4299b410bf6cbd2702f6
16 Ne1c8d18c17114f66a9c036eaee9203ce
17 Nf92acd7a55f64de5b72d95a310ac8dea
18 anzsrc-for:06
19 anzsrc-for:0601
20 schema:author Ne45920be9c8e45c4bccb17a7f0ab3184
21 schema:citation sg:pub.10.1007/s10529-008-9739-9
22 sg:pub.10.1007/s10719-007-9082-8
23 sg:pub.10.1023/b:biry.0000029853.34093.13
24 sg:pub.10.1038/227680a0
25 sg:pub.10.1038/nbt.1976
26 sg:pub.10.1385/mb:16:1:23
27 sg:pub.10.3103/s089141681603006x
28 https://app.dimensions.ai/details/publication/pub.1077157407
29 https://app.dimensions.ai/details/publication/pub.1083127471
30 https://doi.org/10.1002/(sici)1097-0061(199908)15:11<1087::aid-yea436>3.0.co;2-f
31 https://doi.org/10.1002/bit.10616
32 https://doi.org/10.1002/bit.260250717
33 https://doi.org/10.1002/jmr.687
34 https://doi.org/10.1002/pmic.201400199
35 https://doi.org/10.1006/prep.1997.0765
36 https://doi.org/10.1016/0003-2697(87)90369-1
37 https://doi.org/10.1016/0076-6879(88)60109-1
38 https://doi.org/10.1016/j.bbagen.2004.07.001
39 https://doi.org/10.1016/j.biortech.2006.02.009
40 https://doi.org/10.1016/j.carres.2007.10.014
41 https://doi.org/10.1016/j.febslet.2014.03.023
42 https://doi.org/10.1016/j.femsyr.2004.12.011
43 https://doi.org/10.1016/j.str.2004.04.020
44 https://doi.org/10.1016/s0008-6215(96)00325-4
45 https://doi.org/10.1016/s0168-1656(03)00003-8
46 https://doi.org/10.1016/s0958-1669(02)00330-0
47 https://doi.org/10.1021/bp050361o
48 https://doi.org/10.1074/jbc.m208443200
49 https://doi.org/10.1089/ind.2011.7.214
50 https://doi.org/10.1093/glycob/cwh036
51 https://doi.org/10.1099/00221287-136-10-1967
52 https://doi.org/10.1107/s1744309113030005
53 https://doi.org/10.1111/j.1399-3054.1993.tb01778.x
54 https://doi.org/10.1111/j.1470-8744.1999.tb00770.x
55 https://doi.org/10.1111/j.1574-6976.2000.tb00532.x
56 https://doi.org/10.1111/j.1742-4658.2009.07205.x
57 https://doi.org/10.1128/aem.01762-12
58 https://doi.org/10.1128/aem.01922-10
59 https://doi.org/10.1177/1091581813484069
60 https://doi.org/10.3389/fmicb.2014.00281
61 https://doi.org/10.4014/jmb.1109.09031
62 https://doi.org/10.4314/jasem.v10i3.17326
63 schema:datePublished 2017-07
64 schema:datePublishedReg 2017-07-01
65 schema:description A xyloglucanase of the GH74 family was identified in the thermophilic fungus strain Myceliophthora thermophila VKPM F-244, and its gene sequence was optimized for cloning and expression in Pichia pastoris. The recombinant xyloglucanase MtXgh74 exhibited the highest activity toward tamarind seed xyloglucan with a K <sub>M</sub> value of 0.51 ± 0.06 mg/mL. The activities on barley β-glucan and carboxymethylcellulose were about 4 and 2%, respectively, compared to xyloglucan. Maximum xyloglucanase activity was observed at 70-75 °C and pH 6.5. After pre-incubation at 50 °C, pH 6.0 for 3 h, the enzyme retained 100% of its activity. The half-life of MtXgh74 at 60 °C, pH 6.0 was 40 min. In P. pastoris, MtXgh74 was produced in glycosylated form. The enzyme production in a 1 L bioreactor resulted in a yield of 118 U/mL or 5.3 g/L after 51 h fermentation. Kinetic studies of the hydrolysis product formation suggest that MtXgh74 has an endo-processive mode of action. The final products were the standard xyloglucan building blocks XXXG, XXLG, XLXG, and XLLG. Additionally, MtXgh74 hydrolyzed various linkages within the xyloglucan building blocks XXXG, XXLG, and XLXG (except XLLG) producing diverse low molecular weight oligosaccharides which may be identified by MALDI-TOF as XG, XX, XXG/GXX/XGX, XXX, LG, LX/XL, XLX/XXL, LLG, GXXXG, GXLLG, XLLGX. The unique combination of different activities within one enzyme along with its high thermostability and specificity toward xyloglucan makes MtXgh74 a promising candidate enzyme for industrial applications.
66 schema:genre research_article
67 schema:inLanguage en
68 schema:isAccessibleForFree false
69 schema:isPartOf N32cdf5a42f404d1082a82b5fd1edc295
70 N3edab69119fe488dac34c628b3c531e4
71 sg:journal.1083533
72 schema:name Thermostable multifunctional GH74 xyloglucanase from Myceliophthora thermophila: high-level expression in Pichia pastoris and characterization of the recombinant protein
73 schema:pagination 5653-5666
74 schema:productId N258f72b1ea434f04b495e10b16032403
75 N33bf2aea3a6347d2b1ce5b79050767b4
76 N5da69139e3b34d1aa78c25d17251d01d
77 N6e88f678a6a64fa8a056fa9dda3b2b0b
78 Nd1d9169ae8814778bef6bb4c8a5e7f61
79 schema:sameAs https://app.dimensions.ai/details/publication/pub.1085191910
80 https://doi.org/10.1007/s00253-017-8297-2
81 schema:sdDatePublished 2019-04-11T10:21
82 schema:sdLicense https://scigraph.springernature.com/explorer/license/
83 schema:sdPublisher N3bf33ff8f047460798b63a91bd7e3d58
84 schema:url https://link.springer.com/10.1007%2Fs00253-017-8297-2
85 sgo:license sg:explorer/license/
86 sgo:sdDataset articles
87 rdf:type schema:ScholarlyArticle
88 N02cd9e5cc97f44e2abf6796f2be204e3 schema:affiliation https://www.grid.ac/institutes/grid.418697.5
89 schema:familyName Kozlov
90 schema:givenName Dmitriy
91 rdf:type schema:Person
92 N0dc17669ecf443aaa6967a57a7429431 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name Half-Life
94 rdf:type schema:DefinedTerm
95 N0f3c27874a4648648aa1cae238e9d8ac rdf:first sg:person.0653103754.69
96 rdf:rest N29d76ccd2e5f4c3ba0639db47aa6bed7
97 N1244aea4b5ee4ecab436b6bd4322c5e1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
98 schema:name Cloning, Molecular
99 rdf:type schema:DefinedTerm
100 N258f72b1ea434f04b495e10b16032403 schema:name readcube_id
101 schema:value 92cd7393cebadc6a213e9c1da379585eab5149802d35c36a63f0618e9d2d3368
102 rdf:type schema:PropertyValue
103 N26b91beb8c224b69b332d612d9c7fa97 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
104 schema:name Xylans
105 rdf:type schema:DefinedTerm
106 N2712cd2298f240d4ac0cde8457e7c643 rdf:first sg:person.011150256722.54
107 rdf:rest Ncdcb567eb5bf41388450fba406c18621
108 N29d76ccd2e5f4c3ba0639db47aa6bed7 rdf:first sg:person.0725145416.81
109 rdf:rest Ne281707d8bc44626acfe20b1618ef369
110 N32cdf5a42f404d1082a82b5fd1edc295 schema:volumeNumber 101
111 rdf:type schema:PublicationVolume
112 N33bf2aea3a6347d2b1ce5b79050767b4 schema:name pubmed_id
113 schema:value 28477154
114 rdf:type schema:PropertyValue
115 N3bf33ff8f047460798b63a91bd7e3d58 schema:name Springer Nature - SN SciGraph project
116 rdf:type schema:Organization
117 N3edab69119fe488dac34c628b3c531e4 schema:issueNumber 14
118 rdf:type schema:PublicationIssue
119 N431b57431900466c9ed4d79ebf870044 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
120 schema:name Pichia
121 rdf:type schema:DefinedTerm
122 N4c9b31f932e74ba98795be6c44b00173 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
123 schema:name Glycoside Hydrolases
124 rdf:type schema:DefinedTerm
125 N4ee13cea897d4069b08f11a8576d2ad5 rdf:first sg:person.015051330373.06
126 rdf:rest N0f3c27874a4648648aa1cae238e9d8ac
127 N5da69139e3b34d1aa78c25d17251d01d schema:name nlm_unique_id
128 schema:value 8406612
129 rdf:type schema:PropertyValue
130 N6c792fa7686647e0938b4426793051a4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
131 schema:name beta-Glucans
132 rdf:type schema:DefinedTerm
133 N6e88f678a6a64fa8a056fa9dda3b2b0b schema:name doi
134 schema:value 10.1007/s00253-017-8297-2
135 rdf:type schema:PropertyValue
136 N70e2188b4aa04e6f85cd9f3c6dfe0d31 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
137 schema:name Recombinant Proteins
138 rdf:type schema:DefinedTerm
139 N71abef1fbc424b29a69ce483b11d45d3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
140 schema:name Glucans
141 rdf:type schema:DefinedTerm
142 N738741264f4040d69e00917f8284e66d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
143 schema:name Hydrogen-Ion Concentration
144 rdf:type schema:DefinedTerm
145 N8122dc7106be4b239b9e111659819101 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
146 schema:name Temperature
147 rdf:type schema:DefinedTerm
148 N9d513f3da77b41a29457aa3aa86ba5aa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
149 schema:name Enzyme Stability
150 rdf:type schema:DefinedTerm
151 Na166231e771e49b1a99871d63842a556 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
152 schema:name Kinetics
153 rdf:type schema:DefinedTerm
154 Na5370f8ea11e44f7a68e6be1b95ee1fa rdf:first sg:person.0624523277.21
155 rdf:rest Nb224298b76d949599679b6731d3a5b2a
156 Nb224298b76d949599679b6731d3a5b2a rdf:first sg:person.0767160074.28
157 rdf:rest rdf:nil
158 Nc45de68941254fe18cc45a94ea8d652a rdf:first sg:person.01133641461.15
159 rdf:rest N2712cd2298f240d4ac0cde8457e7c643
160 Nca07813a96be450cb92ff23be6a53705 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
161 schema:name Molecular Weight
162 rdf:type schema:DefinedTerm
163 Ncc412f6508ba45ac9fe8b20b9964b2dd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
164 schema:name Substrate Specificity
165 rdf:type schema:DefinedTerm
166 Ncdcb567eb5bf41388450fba406c18621 rdf:first sg:person.01207074472.65
167 rdf:rest Na5370f8ea11e44f7a68e6be1b95ee1fa
168 Nd042bc1a4c2b4299b410bf6cbd2702f6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
169 schema:name Sordariales
170 rdf:type schema:DefinedTerm
171 Nd1d9169ae8814778bef6bb4c8a5e7f61 schema:name dimensions_id
172 schema:value pub.1085191910
173 rdf:type schema:PropertyValue
174 Nd999c61c2318419eb8a25d18751a43ab rdf:first sg:person.011334161173.00
175 rdf:rest Nc45de68941254fe18cc45a94ea8d652a
176 Ne1c8d18c17114f66a9c036eaee9203ce schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
177 schema:name Hydrolysis
178 rdf:type schema:DefinedTerm
179 Ne281707d8bc44626acfe20b1618ef369 rdf:first sg:person.07741220173.38
180 rdf:rest Nec89b0cb56a64d1eaab5a2eabb47117d
181 Ne45920be9c8e45c4bccb17a7f0ab3184 rdf:first sg:person.01366311264.41
182 rdf:rest N4ee13cea897d4069b08f11a8576d2ad5
183 Nec89b0cb56a64d1eaab5a2eabb47117d rdf:first N02cd9e5cc97f44e2abf6796f2be204e3
184 rdf:rest Nd999c61c2318419eb8a25d18751a43ab
185 Nf92acd7a55f64de5b72d95a310ac8dea schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
186 schema:name Carboxymethylcellulose Sodium
187 rdf:type schema:DefinedTerm
188 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
189 schema:name Biological Sciences
190 rdf:type schema:DefinedTerm
191 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
192 schema:name Biochemistry and Cell Biology
193 rdf:type schema:DefinedTerm
194 sg:journal.1083533 schema:issn 0175-7598
195 1432-0614
196 schema:name Applied Microbiology and Biotechnology
197 rdf:type schema:Periodical
198 sg:person.011150256722.54 schema:affiliation https://www.grid.ac/institutes/grid.6936.a
199 schema:familyName Schwarz
200 schema:givenName Wolfgang H.
201 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011150256722.54
202 rdf:type schema:Person
203 sg:person.011334161173.00 schema:affiliation https://www.grid.ac/institutes/grid.418697.5
204 schema:familyName Sakhibgaraeva
205 schema:givenName Liliya
206 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011334161173.00
207 rdf:type schema:Person
208 sg:person.01133641461.15 schema:affiliation https://www.grid.ac/institutes/grid.418697.5
209 schema:familyName Krestyanova
210 schema:givenName Irina
211 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01133641461.15
212 rdf:type schema:Person
213 sg:person.01207074472.65 schema:affiliation https://www.grid.ac/institutes/grid.4886.2
214 schema:familyName Zverlov
215 schema:givenName Vladimir V.
216 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01207074472.65
217 rdf:type schema:Person
218 sg:person.01366311264.41 schema:affiliation https://www.grid.ac/institutes/grid.418697.5
219 schema:familyName Berezina
220 schema:givenName Oksana V.
221 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01366311264.41
222 rdf:type schema:Person
223 sg:person.015051330373.06 schema:affiliation https://www.grid.ac/institutes/grid.6936.a
224 schema:familyName Herlet
225 schema:givenName Jonathan
226 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015051330373.06
227 rdf:type schema:Person
228 sg:person.0624523277.21 schema:affiliation https://www.grid.ac/institutes/grid.6936.a
229 schema:familyName Liebl
230 schema:givenName Wolfgang
231 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0624523277.21
232 rdf:type schema:Person
233 sg:person.0653103754.69 schema:affiliation https://www.grid.ac/institutes/grid.418697.5
234 schema:familyName Rykov
235 schema:givenName Sergey V.
236 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0653103754.69
237 rdf:type schema:Person
238 sg:person.0725145416.81 schema:affiliation https://www.grid.ac/institutes/grid.6936.a
239 schema:familyName Kornberger
240 schema:givenName Petra
241 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0725145416.81
242 rdf:type schema:Person
243 sg:person.0767160074.28 schema:affiliation https://www.grid.ac/institutes/grid.418697.5
244 schema:familyName Yarotsky
245 schema:givenName Sergey V.
246 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0767160074.28
247 rdf:type schema:Person
248 sg:person.07741220173.38 schema:affiliation https://www.grid.ac/institutes/grid.418697.5
249 schema:familyName Zavyalov
250 schema:givenName Artem
251 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07741220173.38
252 rdf:type schema:Person
253 sg:pub.10.1007/s10529-008-9739-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032544790
254 https://doi.org/10.1007/s10529-008-9739-9
255 rdf:type schema:CreativeWork
256 sg:pub.10.1007/s10719-007-9082-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032879408
257 https://doi.org/10.1007/s10719-007-9082-8
258 rdf:type schema:CreativeWork
259 sg:pub.10.1023/b:biry.0000029853.34093.13 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053671139
260 https://doi.org/10.1023/b:biry.0000029853.34093.13
261 rdf:type schema:CreativeWork
262 sg:pub.10.1038/227680a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010419937
263 https://doi.org/10.1038/227680a0
264 rdf:type schema:CreativeWork
265 sg:pub.10.1038/nbt.1976 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002490916
266 https://doi.org/10.1038/nbt.1976
267 rdf:type schema:CreativeWork
268 sg:pub.10.1385/mb:16:1:23 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013013866
269 https://doi.org/10.1385/mb:16:1:23
270 rdf:type schema:CreativeWork
271 sg:pub.10.3103/s089141681603006x schema:sameAs https://app.dimensions.ai/details/publication/pub.1053881651
272 https://doi.org/10.3103/s089141681603006x
273 rdf:type schema:CreativeWork
274 https://app.dimensions.ai/details/publication/pub.1077157407 schema:CreativeWork
275 https://app.dimensions.ai/details/publication/pub.1083127471 schema:CreativeWork
276 https://doi.org/10.1002/(sici)1097-0061(199908)15:11<1087::aid-yea436>3.0.co;2-f schema:sameAs https://app.dimensions.ai/details/publication/pub.1025354919
277 rdf:type schema:CreativeWork
278 https://doi.org/10.1002/bit.10616 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045652318
279 rdf:type schema:CreativeWork
280 https://doi.org/10.1002/bit.260250717 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013563357
281 rdf:type schema:CreativeWork
282 https://doi.org/10.1002/jmr.687 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015247455
283 rdf:type schema:CreativeWork
284 https://doi.org/10.1002/pmic.201400199 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002186635
285 rdf:type schema:CreativeWork
286 https://doi.org/10.1006/prep.1997.0765 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011661480
287 rdf:type schema:CreativeWork
288 https://doi.org/10.1016/0003-2697(87)90369-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032863794
289 rdf:type schema:CreativeWork
290 https://doi.org/10.1016/0076-6879(88)60109-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013265697
291 rdf:type schema:CreativeWork
292 https://doi.org/10.1016/j.bbagen.2004.07.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006012557
293 rdf:type schema:CreativeWork
294 https://doi.org/10.1016/j.biortech.2006.02.009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017868633
295 rdf:type schema:CreativeWork
296 https://doi.org/10.1016/j.carres.2007.10.014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052506393
297 rdf:type schema:CreativeWork
298 https://doi.org/10.1016/j.febslet.2014.03.023 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031978948
299 rdf:type schema:CreativeWork
300 https://doi.org/10.1016/j.femsyr.2004.12.011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040705677
301 rdf:type schema:CreativeWork
302 https://doi.org/10.1016/j.str.2004.04.020 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020607291
303 rdf:type schema:CreativeWork
304 https://doi.org/10.1016/s0008-6215(96)00325-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014941455
305 rdf:type schema:CreativeWork
306 https://doi.org/10.1016/s0168-1656(03)00003-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050369945
307 rdf:type schema:CreativeWork
308 https://doi.org/10.1016/s0958-1669(02)00330-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014666008
309 rdf:type schema:CreativeWork
310 https://doi.org/10.1021/bp050361o schema:sameAs https://app.dimensions.ai/details/publication/pub.1027022017
311 rdf:type schema:CreativeWork
312 https://doi.org/10.1074/jbc.m208443200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049235227
313 rdf:type schema:CreativeWork
314 https://doi.org/10.1089/ind.2011.7.214 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014971996
315 rdf:type schema:CreativeWork
316 https://doi.org/10.1093/glycob/cwh036 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026521040
317 rdf:type schema:CreativeWork
318 https://doi.org/10.1099/00221287-136-10-1967 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060367505
319 rdf:type schema:CreativeWork
320 https://doi.org/10.1107/s1744309113030005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012771977
321 rdf:type schema:CreativeWork
322 https://doi.org/10.1111/j.1399-3054.1993.tb01778.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1034828749
323 rdf:type schema:CreativeWork
324 https://doi.org/10.1111/j.1470-8744.1999.tb00770.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1046776813
325 rdf:type schema:CreativeWork
326 https://doi.org/10.1111/j.1574-6976.2000.tb00532.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1009711357
327 rdf:type schema:CreativeWork
328 https://doi.org/10.1111/j.1742-4658.2009.07205.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1012506154
329 rdf:type schema:CreativeWork
330 https://doi.org/10.1128/aem.01762-12 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040881097
331 rdf:type schema:CreativeWork
332 https://doi.org/10.1128/aem.01922-10 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027430512
333 rdf:type schema:CreativeWork
334 https://doi.org/10.1177/1091581813484069 schema:sameAs https://app.dimensions.ai/details/publication/pub.1063976692
335 rdf:type schema:CreativeWork
336 https://doi.org/10.3389/fmicb.2014.00281 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028492795
337 rdf:type schema:CreativeWork
338 https://doi.org/10.4014/jmb.1109.09031 schema:sameAs https://app.dimensions.ai/details/publication/pub.1071872177
339 rdf:type schema:CreativeWork
340 https://doi.org/10.4314/jasem.v10i3.17326 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013551669
341 rdf:type schema:CreativeWork
342 https://www.grid.ac/institutes/grid.418697.5 schema:alternateName Genetika
343 schema:name State Research Institute of Genetics and Selection of Industrial Microorganisms, 1-st Dorojniy pr. 1, 117545, Moscow, Russian Federation
344 rdf:type schema:Organization
345 https://www.grid.ac/institutes/grid.4886.2 schema:alternateName Russian Academy of Sciences
346 schema:name Department of Microbiology, Technical University Munich, Emil-Ramann-Str. 4, 85354, Freising, Germany
347 Institute of Molecular Genetics, Russian Academy of Science, Kurchatov Sq. 2, 123182, Moscow, Russian Federation
348 rdf:type schema:Organization
349 https://www.grid.ac/institutes/grid.6936.a schema:alternateName Technical University Munich
350 schema:name Department of Microbiology, Technical University Munich, Emil-Ramann-Str. 4, 85354, Freising, Germany
351 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...