P450BM3 fused to phosphite dehydrogenase allows phosphite-driven selective oxidations View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2017-03

AUTHORS

Nina Beyer, Justyna K. Kulig, Anette Bartsch, Martin A. Hayes, Dick B. Janssen, Marco W. Fraaije

ABSTRACT

To facilitate the wider application of the NADPH-dependent P450BM3, we fused the monooxygenase with a phosphite dehydrogenase (PTDH). The resulting monooxygenase-dehydrogenase fusion enzyme acts as a self-sufficient bifunctional catalyst, accepting phosphite as a cheap electron donor for the regeneration of NADPH.The well-expressed fusion enzyme was purified and analyzed in comparison to the parent enzymes. Using lauric acid as substrate for P450BM3, it was found that the fusion enzyme had similar substrate affinity and hydroxylation selectivity while it displayed a significantly higher activity than the non-fused monooxygenase. Phosphite-driven conversions of lauric acid at restricted NADPH concentrations confirmed multiple turnovers of the cofactor. Interestingly, both the fusion enzyme and the native P450BM3 displayed enzyme concentration dependent activity and the fused enzyme reached optimal activity at a lower enzyme concentration. This suggests that the fusion enzyme has an improved tendency to form functional oligomers.To explore the constructed phosphite-driven P450BM3 as a biocatalyst, conversions of the drug compounds omeprazole and rosiglitazone were performed. PTDH-P450BM3 driven by phosphite was found to be more efficient in terms of total turnover when compared with P450BM3 driven by NADPH. The results suggest that PTDH-P450BM3 is an attractive system for use in biocatalytic and drug metabolism studies. More... »

PAGES

2319-2331

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00253-016-7993-7

DOI

http://dx.doi.org/10.1007/s00253-016-7993-7

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1003924221

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/27900443


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