Fisetin yeast-based bio-capsules via osmoporation: effects of process variables on the encapsulation efficiency and internalized fisetin content View Full Text


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Article Info

DATE

2016-06

AUTHORS

Antonio Anchieta de Câmara, Sébastien Dupont, Laurent Beney, Patrick Gervais, Amauri Rosenthal, Roberta Targino Pinto Correia, Márcia Regina da Silva Pedrini

ABSTRACT

Osmoporation is an innovative method that can be used with food-grade yeast cells of Saccharomyces cerevisiae as natural encapsulating matrices. This technique overcomes barriers that difficult encapsulation and enables the internalization of fragile bioactive molecules such as fisetin into yeasts. In the present study, we assessed the effects of concentration, osmotic pressure, and temperature on the encapsulation efficiency (EE) and internalized fisetin content (IF). Two different quantification strategies were investigated: direct extraction (DE) without cell washing or freeze-drying steps and indirect extraction (IE) performed after washings with ethanol and freeze-drying. Our results showed that osmoporation improved EE (33 %) and IF (1.199 mg). The best experimental conditions were found by using DE. High-resolution images showed that the yeast cell envelope was preserved during osmoporation at 30 MPa and 84 % of yeast cells remained viable after treatment. Washing cells with organic solvent led to decreased EE (0.65 %) and IF (0.023 mg). This was probably due to either damages caused to yeast cell envelope or fisetin dragged out of cell. Overall, the results demonstrated the adequacy and relevant biotechnological potential of yeasts as encapsulating matrices for hydrophobic compounds. This fresh biotechnological approach has proven to be a promising tool for the production of bioactive-rich food products. More... »

PAGES

5547-5558

References to SciGraph publications

  • 2001-08. Coupling effects of osmotic pressure and temperature on the viability of Saccharomyces cerevisiae in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2011-12. Antiviral activity of four types of bioflavonoid against dengue virus type-2 in VIROLOGY JOURNAL
  • 2001-07. The effect of osmotic pressure on the membrane fluidity of Saccharomyces cerevisiae at different physiological temperatures in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2012-09. Yeast cells as microcapsules. Analytical tools and process variables in the encapsulation of hydrophobes in S. cerevisiae in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 1989. Electroporation of Cell Membranes in ELECTROPORATION AND ELECTROFUSION IN CELL BIOLOGY
  • 2014-02. Osmoporation: a simple way to internalize hydrophilic molecules into yeast in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2011-01. Safety and regulation of yeasts used for biocontrol or biopreservation in the food or feed chain in ANTONIE VAN LEEUWENHOEK
  • 1999-11. Direct observation of oxidative stress on the cell wall of Saccharomyces cerevisiae strains with atomic force microscopy in MOLECULAR AND CELLULAR BIOCHEMISTRY
  • 2013-08. Encapsulation in a natural, preformed, multi-component and complex capsule: yeast cells in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2003-04. The emerging role for sphingolipids in the eukaryotic heat shock response in CELLULAR AND MOLECULAR LIFE SCIENCES
  • 2010-10. Biophysical properties of Saccharomyces cerevisiae and their relationship with HOG pathway activation in EUROPEAN BIOPHYSICS JOURNAL
  • 2005-11. Antiherpetic activities of flavonoids against herpes simplex virus type 1 (HSV-1) and type 2 (HSV-2)in vitro in ARCHIVES OF PHARMACAL RESEARCH
  • 2008-10. Flavonoids inhibit histamine release and expression of proinflammatory cytokines in mast cells in ARCHIVES OF PHARMACAL RESEARCH
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00253-016-7425-8

    DOI

    http://dx.doi.org/10.1007/s00253-016-7425-8

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1014840400

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/26980099


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