Ontology type: schema:ScholarlyArticle
2016-05
AUTHORSDiego Moricoli, Damiano Cosimo Carbonella, Sabrina Dominici, Valentina Fiori, Maria Cristina Balducci, Clara Guerzoni, Maria Cristina Manara, Michela Pasello, Maria Elena Laguardia, Maurizio Cianfriglia, Katia Scotlandi, Mauro Magnani
ABSTRACTEwing's sarcoma (EWS) is the second most common primary bone tumor in pediatric patients characterized by over expression of CD99. Current management consists in extensive chemotherapy in addition to surgical resection and/or radiation. Recent improvements in treatment are still overshadowed by severe side effects such as toxicity and risk of secondary malignancies; therefore, more effective strategies are urgently needed. The goal of this work was to develop a rapid, inexpensive, and "up-scalable" process of a novel human bivalent single-chain fragment variable diabody (C7 dAbd) directed against CD99, as a new therapeutic approach for EWS. We first investigated different Escherichia coli constructs of C7 dAbd in small-scale studies. Starting from 60 % soluble fraction, we obtained a yield of 25 mg C7 dAbd per liter of bacterial culture with the construct containing pelB signal sequence. In contrast, a low recovery of C7 dAbd was achieved starting from periplasmic inclusion bodies. In order to maximize the yield of C7 dAbd, large-scale fermentation was optimized. We obtained from 75 % soluble fraction 35 mg C7 dAbd per L of cell culture grown in a synthetic media containing 3 g/L of vegetable peptone and 1 g/L of yeast extract. Furthermore, we demonstrated the better efficacy of the cell lysis by homogenization versus periplasmic extraction, in reducing endotoxin level of the C7 dAbd. For gram-scale purification, a direct aligned two-step chromatography cascade based on binding selectivity was developed. Finally, we recovered C7 dAbd with low residual process-related impurities, excellent reactivity, and apoptotic ability against EWS cells. More... »
PAGES3949-3963
http://scigraph.springernature.com/pub.10.1007/s00253-015-7226-5
DOIhttp://dx.doi.org/10.1007/s00253-015-7226-5
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1036728738
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/26685854
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1112",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Oncology and Carcinogenesis",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Medical and Health Sciences",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "12E7 Antigen",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Antineoplastic Agents",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Apoptosis",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Bone Neoplasms",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cell Line, Tumor",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cell Survival",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Escherichia coli",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Humans",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Recombinant Proteins",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Sarcoma, Ewing",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Single-Chain Antibodies",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"name": [
"Diatheva S.R.L, via T.Campanella 1, 61032, Fano, Italy"
],
"type": "Organization"
},
"familyName": "Moricoli",
"givenName": "Diego",
"id": "sg:person.01343465146.38",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01343465146.38"
],
"type": "Person"
},
{
"affiliation": {
"name": [
"Diatheva S.R.L, via T.Campanella 1, 61032, Fano, Italy"
],
"type": "Organization"
},
"familyName": "Carbonella",
"givenName": "Damiano Cosimo",
"id": "sg:person.01276116325.11",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01276116325.11"
],
"type": "Person"
},
{
"affiliation": {
"name": [
"Diatheva S.R.L, via T.Campanella 1, 61032, Fano, Italy"
],
"type": "Organization"
},
"familyName": "Dominici",
"givenName": "Sabrina",
"id": "sg:person.01210363450.49",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01210363450.49"
],
"type": "Person"
},
{
"affiliation": {
"name": [
"Diatheva S.R.L, via T.Campanella 1, 61032, Fano, Italy"
],
"type": "Organization"
},
"familyName": "Fiori",
"givenName": "Valentina",
"id": "sg:person.0575404504.61",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0575404504.61"
],
"type": "Person"
},
{
"affiliation": {
"name": [
"Diatheva S.R.L, via T.Campanella 1, 61032, Fano, Italy"
],
"type": "Organization"
},
"familyName": "Balducci",
"givenName": "Maria Cristina",
"id": "sg:person.01324323024.87",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01324323024.87"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Istituto Ortopedico Rizzoli",
"id": "https://www.grid.ac/institutes/grid.419038.7",
"name": [
"CRS Development of Biomolecular Therapies, Experimental Oncology Laboratory, Istituto Ortopedico Rizzoli, 40136, Bologna, Italy",
"PROMETEO Laboratory, STB, RIT Department, Istituto Ortopedico Rizzoli, 40136, Bologna, Italy"
],
"type": "Organization"
},
"familyName": "Guerzoni",
"givenName": "Clara",
"id": "sg:person.01227066027.74",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01227066027.74"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Istituto Ortopedico Rizzoli",
"id": "https://www.grid.ac/institutes/grid.419038.7",
"name": [
"CRS Development of Biomolecular Therapies, Experimental Oncology Laboratory, Istituto Ortopedico Rizzoli, 40136, Bologna, Italy",
"PROMETEO Laboratory, STB, RIT Department, Istituto Ortopedico Rizzoli, 40136, Bologna, Italy"
],
"type": "Organization"
},
"familyName": "Manara",
"givenName": "Maria Cristina",
"id": "sg:person.01216116770.76",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01216116770.76"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Istituto Ortopedico Rizzoli",
"id": "https://www.grid.ac/institutes/grid.419038.7",
"name": [
"CRS Development of Biomolecular Therapies, Experimental Oncology Laboratory, Istituto Ortopedico Rizzoli, 40136, Bologna, Italy",
"PROMETEO Laboratory, STB, RIT Department, Istituto Ortopedico Rizzoli, 40136, Bologna, Italy"
],
"type": "Organization"
},
"familyName": "Pasello",
"givenName": "Michela",
"id": "sg:person.0607462677.53",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0607462677.53"
],
"type": "Person"
},
{
"affiliation": {
"name": [
"Diatheva S.R.L, via T.Campanella 1, 61032, Fano, Italy"
],
"type": "Organization"
},
"familyName": "Laguardia",
"givenName": "Maria Elena",
"id": "sg:person.01265757041.44",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01265757041.44"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Istituto Superiore di Sanit\u00e0",
"id": "https://www.grid.ac/institutes/grid.416651.1",
"name": [
"Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanit\u00e0, Viale Regina Elena 299, 00161, Rome, Italy"
],
"type": "Organization"
},
"familyName": "Cianfriglia",
"givenName": "Maurizio",
"id": "sg:person.01144451467.41",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01144451467.41"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Istituto Ortopedico Rizzoli",
"id": "https://www.grid.ac/institutes/grid.419038.7",
"name": [
"CRS Development of Biomolecular Therapies, Experimental Oncology Laboratory, Istituto Ortopedico Rizzoli, 40136, Bologna, Italy",
"PROMETEO Laboratory, STB, RIT Department, Istituto Ortopedico Rizzoli, 40136, Bologna, Italy"
],
"type": "Organization"
},
"familyName": "Scotlandi",
"givenName": "Katia",
"id": "sg:person.01327374561.76",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01327374561.76"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Urbino",
"id": "https://www.grid.ac/institutes/grid.12711.34",
"name": [
"Department of Biomolecular Science, Section of Biochemistry and Molecular Biology University of Urbino \u201cCarlo Bo\u201d, Via Saffi 2, 61029, Urbino, Italy"
],
"type": "Organization"
},
"familyName": "Magnani",
"givenName": "Mauro",
"id": "sg:person.0752252542.98",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0752252542.98"
],
"type": "Person"
}
],
"citation": [
{
"id": "https://doi.org/10.1172/jci36667",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000562033"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1172/jci36667",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000562033"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/978-3-319-07323-1_5",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1001314093",
"https://doi.org/10.1007/978-3-319-07323-1_5"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/1615-9861(200109)1:9<1133::aid-prot1133>3.0.co;2-s",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1002328246"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1097/00000478-199405000-00008",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1002567455"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1097/00000478-199405000-00008",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1002567455"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.pep.2005.04.015",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1003856785"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/978-1-60761-913-0_9",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1004892945",
"https://doi.org/10.1007/978-1-60761-913-0_9"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/978-1-60761-913-0_9",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1004892945",
"https://doi.org/10.1007/978-1-60761-913-0_9"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1042/bj2900723",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1006048637"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1042/bj2900723",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1006048637"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0378-1119(03)00401-3",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1009476659"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0378-1119(03)00401-3",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1009476659"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/227680a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1010419937",
"https://doi.org/10.1038/227680a0"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0022-1759(02)00036-4",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1010786034"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1146/annurev.iy.12.040194.002245",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1011126452"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.76.9.4350",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1011704795"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/sj.bjc.6603925",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1013164393",
"https://doi.org/10.1038/sj.bjc.6603925"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/978-1-61779-974-7_18",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1016957971",
"https://doi.org/10.1007/978-1-61779-974-7_18"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.jaci.2005.08.003",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1017465172"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.3389/fimmu.2013.00217",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1018469179"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/35101072",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1018512036",
"https://doi.org/10.1038/35101072"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/35101072",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1018512036",
"https://doi.org/10.1038/35101072"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt1142",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1018849649",
"https://doi.org/10.1038/nbt1142"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt1142",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1018849649",
"https://doi.org/10.1038/nbt1142"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/bioinformatics/bts493",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1019195363"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.3389/fonc.2014.00063",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1021070018"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1200/jco.2010.32.7270",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1026289504"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/ni749",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1026384666",
"https://doi.org/10.1038/ni749"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/ni749",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1026384666",
"https://doi.org/10.1038/ni749"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.pep.2013.10.009",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1027031671"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/stem.5530080644",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1027819308"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/stem.5530080644",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1027819308"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1158/1078-0432.ccr-14-0492",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1028861296"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nprot.2009.2",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1029454980",
"https://doi.org/10.1038/nprot.2009.2"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/cncr.27493",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1030952031"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1158/0008-5472.can-04-2008",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1031784123"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.canep.2014.12.012",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1033662823"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0167-6296(02)00126-1",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1034082462"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0167-6296(02)00126-1",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1034082462"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1186/1475-2859-8-17",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1034460564",
"https://doi.org/10.1186/1475-2859-8-17"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s10295-011-1082-9",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1034745235",
"https://doi.org/10.1007/s10295-011-1082-9"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/978-1-60327-302-2_16",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1036244839",
"https://doi.org/10.1007/978-1-60327-302-2_16"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/978-1-60327-302-2_16",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1036244839",
"https://doi.org/10.1007/978-1-60327-302-2_16"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/jso.23866",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1036922304"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.2174/138920111794295693",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1037420616"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt0409-331",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1037500517",
"https://doi.org/10.1038/nbt0409-331"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt0409-331",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1037500517",
"https://doi.org/10.1038/nbt0409-331"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1200/jco.2009.27.0421",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044783492"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/j.1365-2184.2009.00630.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1045832826"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/j.1365-2184.2009.00630.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1045832826"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/pbc.10472",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1046956165"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1097/coc.0000000000000176",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1049612511"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1097/coc.0000000000000176",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1049612511"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.jim.2014.04.012",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1050117210"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/j.ejca.2005.09.015",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1052222871"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1080/19420862.2015.1022694",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1058413289"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/hmg/2.4.417",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1059644338"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1126/science.4001944",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1062623727"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1160/th08-12-0816",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1063291933"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.2174/1389201011314040011",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1069176663"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.4161/19420862.2015.988944",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1072288700"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.4161/mabs.3.5.16983",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1072305855"
],
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1074557546",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1074708138",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1076991277",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1078142397",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1082399587",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1082884167",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1083138895",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1083327363",
"type": "CreativeWork"
}
],
"datePublished": "2016-05",
"datePublishedReg": "2016-05-01",
"description": "Ewing's sarcoma (EWS) is the second most common primary bone tumor in pediatric patients characterized by over expression of CD99. Current management consists in extensive chemotherapy in addition to surgical resection and/or radiation. Recent improvements in treatment are still overshadowed by severe side effects such as toxicity and risk of secondary malignancies; therefore, more effective strategies are urgently needed. The goal of this work was to develop a rapid, inexpensive, and \"up-scalable\" process of a novel human bivalent single-chain fragment variable diabody (C7 dAbd) directed against CD99, as a new therapeutic approach for EWS. We first investigated different Escherichia coli constructs of C7 dAbd in small-scale studies. Starting from 60\u00a0% soluble fraction, we obtained a yield of 25\u00a0mg C7 dAbd per liter of bacterial culture with the construct containing pelB signal sequence. In contrast, a low recovery of C7 dAbd was achieved starting from periplasmic inclusion bodies. In order to maximize the yield of C7 dAbd, large-scale fermentation was optimized. We obtained from 75\u00a0% soluble fraction 35\u00a0mg C7 dAbd per L of cell culture grown in a synthetic media containing 3\u00a0g/L of vegetable peptone and 1\u00a0g/L of yeast extract. Furthermore, we demonstrated the better efficacy of the cell lysis by homogenization versus periplasmic extraction, in reducing endotoxin level of the C7 dAbd. For gram-scale purification, a direct aligned two-step chromatography cascade based on binding selectivity was developed. Finally, we recovered C7 dAbd with low residual process-related impurities, excellent reactivity, and apoptotic ability against EWS cells. ",
"genre": "research_article",
"id": "sg:pub.10.1007/s00253-015-7226-5",
"inLanguage": [
"en"
],
"isAccessibleForFree": false,
"isPartOf": [
{
"id": "sg:journal.1083533",
"issn": [
"0175-7598",
"1432-0614"
],
"name": "Applied Microbiology and Biotechnology",
"type": "Periodical"
},
{
"issueNumber": "9",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "100"
}
],
"name": "Process development of a human recombinant diabody expressed in E. coli: engagement of CD99-induced apoptosis for target therapy in Ewing\u2019s sarcoma",
"pagination": "3949-3963",
"productId": [
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"aef625ffb651a02d7fbf529e87656204e773caf65d83dc313e8198fd089f2a98"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"26685854"
]
},
{
"name": "nlm_unique_id",
"type": "PropertyValue",
"value": [
"8406612"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/s00253-015-7226-5"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1036728738"
]
}
],
"sameAs": [
"https://doi.org/10.1007/s00253-015-7226-5",
"https://app.dimensions.ai/details/publication/pub.1036728738"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-10T21:30",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8687_00000482.jsonl",
"type": "ScholarlyArticle",
"url": "http://link.springer.com/10.1007/s00253-015-7226-5"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00253-015-7226-5'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00253-015-7226-5'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00253-015-7226-5'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00253-015-7226-5'
This table displays all metadata directly associated to this object as RDF triples.
385 TRIPLES
21 PREDICATES
97 URIs
32 LITERALS
20 BLANK NODES