Osmoporation: a simple way to internalize hydrophilic molecules into yeast View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2014-02

AUTHORS

Marcia Regina da Silva Pedrini, Sebastien Dupont, Antonio de Anchieta Câmara, Laurent Beney, Patrick Gervais

ABSTRACT

Internalization of hydrophilic molecules into yeast cytosol is required for different applications such as cell transformation or preservation of water soluble components by bioencapsulation. However, these molecules are not able to cross the plasma membrane and strategies have to be developed. Recent works revealed that osmotic perturbations could induce non-lethal transient permeabilization of the plasma membrane. In this work, we endeavored to clarify the phenomenon of permeabilization during rehydration after a mild hyperosmotic perturbation in order to evaluate the possibility of hydrophilic molecule internalization in yeast by this treatment. Rehydration step is particularly interesting because the large entry of water into the cells could help the internalization of molecules. The internalization of a fluorescent molecule [fluorescein isothiocyanate Dextran (FITC-Dextran), 20 kDa], added during the rehydration after a sublethal hyperosmotic treatment, was studied in Saccharomyces cerevisiae yeast cells. The internalization kinetic and the localization of the fluorescent molecules were studied by flow cytometry and fluorescence confocal microscopy. Our results show that the rehydration leads to the rapid internalization of FITC-Dextran due to a transient plasma membrane permeabilization. Thus, osmoporation, i.e. plasma membrane poration by modifications of osmotic pressure of the extracellular medium, could be a new and simple way to deliver molecules of particular interest into yeasts. More... »

PAGES

1271-1280

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00253-013-5386-8

DOI

http://dx.doi.org/10.1007/s00253-013-5386-8

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1015444591

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24318006


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cell Membrane Permeability", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Dextrans", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Endocytosis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Flow Cytometry", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fluorescein-5-isothiocyanate", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hydrophobic and Hydrophilic Interactions", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Osmotic Pressure", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Saccharomyces cerevisiae", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Staining and Labeling", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Federal University of Rio Grande do Norte", 
          "id": "https://www.grid.ac/institutes/grid.411233.6", 
          "name": [
            "UMR Proc\u00e9d\u00e9s Alimentaires et Microbiologiques, Universit\u00e9 de Bourgogne/AgroSup Dijon, 1, esplanade Erasme, 21000, Dijon, France", 
            "Laborat\u00f3rio de Bioprocessos, Universidade Federal do Rio Grande do Norte, Lagoa Nova, 59078-900, Natal, RN, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "da Silva Pedrini", 
        "givenName": "Marcia Regina", 
        "id": "sg:person.01131206611.54", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01131206611.54"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Proc\u00e9d\u00e9s Alimentaires et Microbiologiques", 
          "id": "https://www.grid.ac/institutes/grid.464129.c", 
          "name": [
            "UMR Proc\u00e9d\u00e9s Alimentaires et Microbiologiques, Universit\u00e9 de Bourgogne/AgroSup Dijon, 1, esplanade Erasme, 21000, Dijon, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Dupont", 
        "givenName": "Sebastien", 
        "id": "sg:person.0751140473.04", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0751140473.04"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Federal University of Rio Grande do Norte", 
          "id": "https://www.grid.ac/institutes/grid.411233.6", 
          "name": [
            "UMR Proc\u00e9d\u00e9s Alimentaires et Microbiologiques, Universit\u00e9 de Bourgogne/AgroSup Dijon, 1, esplanade Erasme, 21000, Dijon, France", 
            "Laborat\u00f3rio de Bioprocessos, Universidade Federal do Rio Grande do Norte, Lagoa Nova, 59078-900, Natal, RN, Brazil"
          ], 
          "type": "Organization"
        }, 
        "familyName": "de Anchieta C\u00e2mara", 
        "givenName": "Antonio", 
        "id": "sg:person.0675547360.40", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0675547360.40"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Proc\u00e9d\u00e9s Alimentaires et Microbiologiques", 
          "id": "https://www.grid.ac/institutes/grid.464129.c", 
          "name": [
            "UMR Proc\u00e9d\u00e9s Alimentaires et Microbiologiques, Universit\u00e9 de Bourgogne/AgroSup Dijon, 1, esplanade Erasme, 21000, Dijon, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Beney", 
        "givenName": "Laurent", 
        "id": "sg:person.01320562152.37", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01320562152.37"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Proc\u00e9d\u00e9s Alimentaires et Microbiologiques", 
          "id": "https://www.grid.ac/institutes/grid.464129.c", 
          "name": [
            "UMR Proc\u00e9d\u00e9s Alimentaires et Microbiologiques, Universit\u00e9 de Bourgogne/AgroSup Dijon, 1, esplanade Erasme, 21000, Dijon, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Gervais", 
        "givenName": "Patrick", 
        "id": "sg:person.01035655265.67", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01035655265.67"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1161/circresaha.108.183806", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002993110"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1161/circresaha.108.183806", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002993110"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ijpharm.2007.07.044", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004018144"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.081065198", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004818121"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.foodhyd.2006.12.013", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007146298"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00326575", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009251986", 
          "https://doi.org/10.1007/bf00326575"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0167-7012(97)00975-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009431110"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/yea.320100702", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011457792"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/yea.320100702", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011457792"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1558-5646.2012.01667.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014467922"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.105.5.1981", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015757790"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1432-1327.2001.02364.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015911414"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.foodchem.2010.09.071", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016084441"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0014-5793(88)80791-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017487406"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbamem.2008.06.006", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018106821"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.128.5.779", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019476600"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jfoodeng.2006.06.038", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021358037"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/(sici)1097-0320(20000401)39:4<243::aid-cyto1>3.0.co;2-v", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022353908"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0302-4598(93)86107-c", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022657163"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1462-2920.2010.02317.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022937170"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.3109/02652049809008259", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023449375"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-012-4014-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028045129", 
          "https://doi.org/10.1007/s00253-012-4014-3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-013-5044-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028307023", 
          "https://doi.org/10.1007/s00253-013-5044-1"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0005-2736(95)00181-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030786490"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s002530000583", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035973675", 
          "https://doi.org/10.1007/s002530000583"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.foodchem.2010.09.063", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036681579"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4161/bbug.1.6.13257", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037672309"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-012-4127-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039875999", 
          "https://doi.org/10.1007/s00253-012-4127-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/1097-0061(20000915)16:12<1107::aid-yea608>3.0.co;2-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042392824"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/yea.320060605", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046583459"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/yea.320060605", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1046583459"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbamem.2010.11.012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047882710"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbamem.2010.01.015", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049938670"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/jf0507893", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055903814"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/jf0507893", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055903814"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074960226", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1081686296", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2014-02", 
    "datePublishedReg": "2014-02-01", 
    "description": "Internalization of hydrophilic molecules into yeast cytosol is required for different applications such as cell transformation or preservation of water soluble components by bioencapsulation. However, these molecules are not able to cross the plasma membrane and strategies have to be developed. Recent works revealed that osmotic perturbations could induce non-lethal transient permeabilization of the plasma membrane. In this work, we endeavored to clarify the phenomenon of permeabilization during rehydration after a mild hyperosmotic perturbation in order to evaluate the possibility of hydrophilic molecule internalization in yeast by this treatment. Rehydration step is particularly interesting because the large entry of water into the cells could help the internalization of molecules. The internalization of a fluorescent molecule [fluorescein isothiocyanate Dextran (FITC-Dextran), 20 kDa], added during the rehydration after a sublethal hyperosmotic treatment, was studied in Saccharomyces cerevisiae yeast cells. The internalization kinetic and the localization of the fluorescent molecules were studied by flow cytometry and fluorescence confocal microscopy. Our results show that the rehydration leads to the rapid internalization of FITC-Dextran due to a transient plasma membrane permeabilization. Thus, osmoporation, i.e. plasma membrane poration by modifications of osmotic pressure of the extracellular medium, could be a new and simple way to deliver molecules of particular interest into yeasts.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s00253-013-5386-8", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1083533", 
        "issn": [
          "0175-7598", 
          "1432-0614"
        ], 
        "name": "Applied Microbiology and Biotechnology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "3", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "98"
      }
    ], 
    "name": "Osmoporation: a simple way to internalize hydrophilic molecules into yeast", 
    "pagination": "1271-1280", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "49963c27f32c161866713f2a93c60c7c8f9d8d6c12dc1001fb90ccc277d97963"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "24318006"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "8406612"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00253-013-5386-8"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1015444591"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00253-013-5386-8", 
      "https://app.dimensions.ai/details/publication/pub.1015444591"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T13:07", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8659_00000487.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/s00253-013-5386-8"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00253-013-5386-8'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00253-013-5386-8'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00253-013-5386-8'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00253-013-5386-8'


 

This table displays all metadata directly associated to this object as RDF triples.

239 TRIPLES      21 PREDICATES      71 URIs      30 LITERALS      18 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00253-013-5386-8 schema:about N00e4d03bc0334b11a2d59d1581a03e6f
2 N2c1d5aff8cf94405bb299276bb456e06
3 N40a50156ccab494ab90e844aed957f49
4 N88652fb516af4aaf9f267f78f0778c0d
5 N965b6a17b3114837857e79f71cbd47be
6 N9806fc78c65b4af9a72400ef35910413
7 N99c7078993514f7588d0449114efad86
8 Nd189a055ebb44b8f88098728bf779981
9 Nfb381dabb040406db130660c4d9f021a
10 anzsrc-for:06
11 anzsrc-for:0601
12 schema:author N2c52a83c9cb344ea8b9c6a3914b7606d
13 schema:citation sg:pub.10.1007/bf00326575
14 sg:pub.10.1007/s00253-012-4014-3
15 sg:pub.10.1007/s00253-012-4127-8
16 sg:pub.10.1007/s00253-013-5044-1
17 sg:pub.10.1007/s002530000583
18 https://app.dimensions.ai/details/publication/pub.1074960226
19 https://app.dimensions.ai/details/publication/pub.1081686296
20 https://doi.org/10.1002/(sici)1097-0320(20000401)39:4<243::aid-cyto1>3.0.co;2-v
21 https://doi.org/10.1002/1097-0061(20000915)16:12<1107::aid-yea608>3.0.co;2-3
22 https://doi.org/10.1002/yea.320060605
23 https://doi.org/10.1002/yea.320100702
24 https://doi.org/10.1016/0005-2736(95)00181-6
25 https://doi.org/10.1016/0014-5793(88)80791-9
26 https://doi.org/10.1016/0302-4598(93)86107-c
27 https://doi.org/10.1016/j.bbamem.2008.06.006
28 https://doi.org/10.1016/j.bbamem.2010.01.015
29 https://doi.org/10.1016/j.bbamem.2010.11.012
30 https://doi.org/10.1016/j.foodchem.2010.09.063
31 https://doi.org/10.1016/j.foodchem.2010.09.071
32 https://doi.org/10.1016/j.foodhyd.2006.12.013
33 https://doi.org/10.1016/j.ijpharm.2007.07.044
34 https://doi.org/10.1016/j.jfoodeng.2006.06.038
35 https://doi.org/10.1016/s0167-7012(97)00975-5
36 https://doi.org/10.1021/jf0507893
37 https://doi.org/10.1046/j.1432-1327.2001.02364.x
38 https://doi.org/10.1073/pnas.081065198
39 https://doi.org/10.1083/jcb.105.5.1981
40 https://doi.org/10.1083/jcb.128.5.779
41 https://doi.org/10.1111/j.1462-2920.2010.02317.x
42 https://doi.org/10.1111/j.1558-5646.2012.01667.x
43 https://doi.org/10.1161/circresaha.108.183806
44 https://doi.org/10.3109/02652049809008259
45 https://doi.org/10.4161/bbug.1.6.13257
46 schema:datePublished 2014-02
47 schema:datePublishedReg 2014-02-01
48 schema:description Internalization of hydrophilic molecules into yeast cytosol is required for different applications such as cell transformation or preservation of water soluble components by bioencapsulation. However, these molecules are not able to cross the plasma membrane and strategies have to be developed. Recent works revealed that osmotic perturbations could induce non-lethal transient permeabilization of the plasma membrane. In this work, we endeavored to clarify the phenomenon of permeabilization during rehydration after a mild hyperosmotic perturbation in order to evaluate the possibility of hydrophilic molecule internalization in yeast by this treatment. Rehydration step is particularly interesting because the large entry of water into the cells could help the internalization of molecules. The internalization of a fluorescent molecule [fluorescein isothiocyanate Dextran (FITC-Dextran), 20 kDa], added during the rehydration after a sublethal hyperosmotic treatment, was studied in Saccharomyces cerevisiae yeast cells. The internalization kinetic and the localization of the fluorescent molecules were studied by flow cytometry and fluorescence confocal microscopy. Our results show that the rehydration leads to the rapid internalization of FITC-Dextran due to a transient plasma membrane permeabilization. Thus, osmoporation, i.e. plasma membrane poration by modifications of osmotic pressure of the extracellular medium, could be a new and simple way to deliver molecules of particular interest into yeasts.
49 schema:genre research_article
50 schema:inLanguage en
51 schema:isAccessibleForFree false
52 schema:isPartOf N19bd03305b0f474498b0a73d359af7f2
53 N1d50a646860d4920be8a9f90385c6599
54 sg:journal.1083533
55 schema:name Osmoporation: a simple way to internalize hydrophilic molecules into yeast
56 schema:pagination 1271-1280
57 schema:productId N258bf4535f2e421094c9271f5eb9f642
58 N5e4c29fb2fef45a6a7801252d309a17d
59 Nac9916c4b6ed45e18891a79d5223cdfe
60 Nbbca48264e4e44a08f595602170ca5b4
61 Nc9a3049a497242e5a62732eac4382544
62 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015444591
63 https://doi.org/10.1007/s00253-013-5386-8
64 schema:sdDatePublished 2019-04-10T13:07
65 schema:sdLicense https://scigraph.springernature.com/explorer/license/
66 schema:sdPublisher N0b9653c58a0a48b386c685c57934e74a
67 schema:url http://link.springer.com/10.1007/s00253-013-5386-8
68 sgo:license sg:explorer/license/
69 sgo:sdDataset articles
70 rdf:type schema:ScholarlyArticle
71 N00e4d03bc0334b11a2d59d1581a03e6f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
72 schema:name Cell Membrane Permeability
73 rdf:type schema:DefinedTerm
74 N0ad4f549f2e04ba2859c7a9d60a09812 rdf:first sg:person.01035655265.67
75 rdf:rest rdf:nil
76 N0b9653c58a0a48b386c685c57934e74a schema:name Springer Nature - SN SciGraph project
77 rdf:type schema:Organization
78 N19bd03305b0f474498b0a73d359af7f2 schema:issueNumber 3
79 rdf:type schema:PublicationIssue
80 N1d50a646860d4920be8a9f90385c6599 schema:volumeNumber 98
81 rdf:type schema:PublicationVolume
82 N258bf4535f2e421094c9271f5eb9f642 schema:name pubmed_id
83 schema:value 24318006
84 rdf:type schema:PropertyValue
85 N2c1d5aff8cf94405bb299276bb456e06 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
86 schema:name Dextrans
87 rdf:type schema:DefinedTerm
88 N2c52a83c9cb344ea8b9c6a3914b7606d rdf:first sg:person.01131206611.54
89 rdf:rest Nfed1556a3ef54d23b6aa08fd714e9bcd
90 N40a50156ccab494ab90e844aed957f49 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
91 schema:name Fluorescein-5-isothiocyanate
92 rdf:type schema:DefinedTerm
93 N5e4c29fb2fef45a6a7801252d309a17d schema:name doi
94 schema:value 10.1007/s00253-013-5386-8
95 rdf:type schema:PropertyValue
96 N88652fb516af4aaf9f267f78f0778c0d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
97 schema:name Staining and Labeling
98 rdf:type schema:DefinedTerm
99 N965b6a17b3114837857e79f71cbd47be schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
100 schema:name Flow Cytometry
101 rdf:type schema:DefinedTerm
102 N9806fc78c65b4af9a72400ef35910413 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
103 schema:name Endocytosis
104 rdf:type schema:DefinedTerm
105 N99c7078993514f7588d0449114efad86 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
106 schema:name Hydrophobic and Hydrophilic Interactions
107 rdf:type schema:DefinedTerm
108 Na99b1bdc56fc4a7ba4b8eb77f063bb17 rdf:first sg:person.01320562152.37
109 rdf:rest N0ad4f549f2e04ba2859c7a9d60a09812
110 Nac9916c4b6ed45e18891a79d5223cdfe schema:name nlm_unique_id
111 schema:value 8406612
112 rdf:type schema:PropertyValue
113 Nbbca48264e4e44a08f595602170ca5b4 schema:name readcube_id
114 schema:value 49963c27f32c161866713f2a93c60c7c8f9d8d6c12dc1001fb90ccc277d97963
115 rdf:type schema:PropertyValue
116 Nc9a3049a497242e5a62732eac4382544 schema:name dimensions_id
117 schema:value pub.1015444591
118 rdf:type schema:PropertyValue
119 Nd189a055ebb44b8f88098728bf779981 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
120 schema:name Saccharomyces cerevisiae
121 rdf:type schema:DefinedTerm
122 Ne50eba1f0af24cada05d10000840bfe3 rdf:first sg:person.0675547360.40
123 rdf:rest Na99b1bdc56fc4a7ba4b8eb77f063bb17
124 Nfb381dabb040406db130660c4d9f021a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
125 schema:name Osmotic Pressure
126 rdf:type schema:DefinedTerm
127 Nfed1556a3ef54d23b6aa08fd714e9bcd rdf:first sg:person.0751140473.04
128 rdf:rest Ne50eba1f0af24cada05d10000840bfe3
129 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
130 schema:name Biological Sciences
131 rdf:type schema:DefinedTerm
132 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
133 schema:name Biochemistry and Cell Biology
134 rdf:type schema:DefinedTerm
135 sg:journal.1083533 schema:issn 0175-7598
136 1432-0614
137 schema:name Applied Microbiology and Biotechnology
138 rdf:type schema:Periodical
139 sg:person.01035655265.67 schema:affiliation https://www.grid.ac/institutes/grid.464129.c
140 schema:familyName Gervais
141 schema:givenName Patrick
142 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01035655265.67
143 rdf:type schema:Person
144 sg:person.01131206611.54 schema:affiliation https://www.grid.ac/institutes/grid.411233.6
145 schema:familyName da Silva Pedrini
146 schema:givenName Marcia Regina
147 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01131206611.54
148 rdf:type schema:Person
149 sg:person.01320562152.37 schema:affiliation https://www.grid.ac/institutes/grid.464129.c
150 schema:familyName Beney
151 schema:givenName Laurent
152 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01320562152.37
153 rdf:type schema:Person
154 sg:person.0675547360.40 schema:affiliation https://www.grid.ac/institutes/grid.411233.6
155 schema:familyName de Anchieta Câmara
156 schema:givenName Antonio
157 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0675547360.40
158 rdf:type schema:Person
159 sg:person.0751140473.04 schema:affiliation https://www.grid.ac/institutes/grid.464129.c
160 schema:familyName Dupont
161 schema:givenName Sebastien
162 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0751140473.04
163 rdf:type schema:Person
164 sg:pub.10.1007/bf00326575 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009251986
165 https://doi.org/10.1007/bf00326575
166 rdf:type schema:CreativeWork
167 sg:pub.10.1007/s00253-012-4014-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028045129
168 https://doi.org/10.1007/s00253-012-4014-3
169 rdf:type schema:CreativeWork
170 sg:pub.10.1007/s00253-012-4127-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039875999
171 https://doi.org/10.1007/s00253-012-4127-8
172 rdf:type schema:CreativeWork
173 sg:pub.10.1007/s00253-013-5044-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028307023
174 https://doi.org/10.1007/s00253-013-5044-1
175 rdf:type schema:CreativeWork
176 sg:pub.10.1007/s002530000583 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035973675
177 https://doi.org/10.1007/s002530000583
178 rdf:type schema:CreativeWork
179 https://app.dimensions.ai/details/publication/pub.1074960226 schema:CreativeWork
180 https://app.dimensions.ai/details/publication/pub.1081686296 schema:CreativeWork
181 https://doi.org/10.1002/(sici)1097-0320(20000401)39:4<243::aid-cyto1>3.0.co;2-v schema:sameAs https://app.dimensions.ai/details/publication/pub.1022353908
182 rdf:type schema:CreativeWork
183 https://doi.org/10.1002/1097-0061(20000915)16:12<1107::aid-yea608>3.0.co;2-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042392824
184 rdf:type schema:CreativeWork
185 https://doi.org/10.1002/yea.320060605 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046583459
186 rdf:type schema:CreativeWork
187 https://doi.org/10.1002/yea.320100702 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011457792
188 rdf:type schema:CreativeWork
189 https://doi.org/10.1016/0005-2736(95)00181-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030786490
190 rdf:type schema:CreativeWork
191 https://doi.org/10.1016/0014-5793(88)80791-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017487406
192 rdf:type schema:CreativeWork
193 https://doi.org/10.1016/0302-4598(93)86107-c schema:sameAs https://app.dimensions.ai/details/publication/pub.1022657163
194 rdf:type schema:CreativeWork
195 https://doi.org/10.1016/j.bbamem.2008.06.006 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018106821
196 rdf:type schema:CreativeWork
197 https://doi.org/10.1016/j.bbamem.2010.01.015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049938670
198 rdf:type schema:CreativeWork
199 https://doi.org/10.1016/j.bbamem.2010.11.012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047882710
200 rdf:type schema:CreativeWork
201 https://doi.org/10.1016/j.foodchem.2010.09.063 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036681579
202 rdf:type schema:CreativeWork
203 https://doi.org/10.1016/j.foodchem.2010.09.071 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016084441
204 rdf:type schema:CreativeWork
205 https://doi.org/10.1016/j.foodhyd.2006.12.013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007146298
206 rdf:type schema:CreativeWork
207 https://doi.org/10.1016/j.ijpharm.2007.07.044 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004018144
208 rdf:type schema:CreativeWork
209 https://doi.org/10.1016/j.jfoodeng.2006.06.038 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021358037
210 rdf:type schema:CreativeWork
211 https://doi.org/10.1016/s0167-7012(97)00975-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009431110
212 rdf:type schema:CreativeWork
213 https://doi.org/10.1021/jf0507893 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055903814
214 rdf:type schema:CreativeWork
215 https://doi.org/10.1046/j.1432-1327.2001.02364.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1015911414
216 rdf:type schema:CreativeWork
217 https://doi.org/10.1073/pnas.081065198 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004818121
218 rdf:type schema:CreativeWork
219 https://doi.org/10.1083/jcb.105.5.1981 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015757790
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1083/jcb.128.5.779 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019476600
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1111/j.1462-2920.2010.02317.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1022937170
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1111/j.1558-5646.2012.01667.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1014467922
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1161/circresaha.108.183806 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002993110
228 rdf:type schema:CreativeWork
229 https://doi.org/10.3109/02652049809008259 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023449375
230 rdf:type schema:CreativeWork
231 https://doi.org/10.4161/bbug.1.6.13257 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037672309
232 rdf:type schema:CreativeWork
233 https://www.grid.ac/institutes/grid.411233.6 schema:alternateName Federal University of Rio Grande do Norte
234 schema:name Laboratório de Bioprocessos, Universidade Federal do Rio Grande do Norte, Lagoa Nova, 59078-900, Natal, RN, Brazil
235 UMR Procédés Alimentaires et Microbiologiques, Université de Bourgogne/AgroSup Dijon, 1, esplanade Erasme, 21000, Dijon, France
236 rdf:type schema:Organization
237 https://www.grid.ac/institutes/grid.464129.c schema:alternateName Procédés Alimentaires et Microbiologiques
238 schema:name UMR Procédés Alimentaires et Microbiologiques, Université de Bourgogne/AgroSup Dijon, 1, esplanade Erasme, 21000, Dijon, France
239 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...