Bioengineering radioresistance by overproduction of RPA, a mammalian-type single-stranded DNA-binding protein, in a halophilic archaeon View Full Text


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Article Info

DATE

2013-11-29

AUTHORS

Ram Karan, Priya DasSarma, Elizabeth Balcer-Kubiczek, Rueyhung Roc Weng, Chen-Chung Liao, David R. Goodlett, Wailap Victor Ng, Shiladitya DasSarma

ABSTRACT

Halobacterium sp. NRC-1 is a wild-type extremophilic microbe that is naturally tolerant to high levels of ionizing radiation. Mutants of strain NRC-1 with even higher levels of resistance to ionizing radiation, named RAD, were previously isolated after selecting survival to extremely high doses of ionizing radiation. These RAD mutants displayed higher transcription levels for the rfa3 operon, coding two subunits of the RPA-like putative single-stranded binding protein, rfa3 and rfa8, and a third downstream gene, ral. In order to bioengineer cells with increased tolerance to ionizing radiation and further explore the genetic basis of the RAD phenotype, we placed the rfa3 operon under control of the gvpA promoter in a Halobacterium expression plasmid, pDRK1. When pDRK1 was introduced into the wild-type NRC-1 strain, overproduction of the Rfa3 and Rfa8 proteins was observed by Western blotting and proteomic analysis. The Halobacterium strains expressing Rfa3 and Rfa8 also displayed improved survival after exposure to ionizing radiation, similar to the RAD mutants, when compared to wild-type strain NRC-1. The Rfa3 and Rfa8 proteins co-purified by affinity chromatography on single-stranded DNA cellulose columns, confirming the ability of the proteins to bind to single-stranded DNA as well as their relative abundance in the wild-type, RAD mutants, and rfa3 operon overexpression strains. These results clearly establish that overexpression of haloarchaeal RPA promotes ionizing radiation resistance in Halobacterium sp. NRC-1 and that the Rfa3 and Rfa8 subunits bind single-stranded DNA. Bioengineering cells with increased levels of ionizing radiation resistance may have potential value in medical and environmental applications. More... »

PAGES

1737-1747

References to SciGraph publications

  • 2004-12-10. Integration with the human genome of peptide sequences obtained by high-throughput mass spectrometry in GENOME BIOLOGY
  • 2007-02-27. Target-decoy search strategy for increased confidence in large-scale protein identifications by mass spectrometry in NATURE METHODS
  • 2007-06-08. Essential and non-essential DNA replication genes in the model halophilic Archaeon, Halobacterium sp. NRC-1 in BMC GENOMIC DATA
  • 2006-09-13. The uvrA, uvrB and uvrC genes are required for repair of ultraviolet light induced DNA photoproducts in Halobacterium sp. NRC-1 in AQUATIC BIOSYSTEMS
  • 2007-07-25. Transcriptional profiling of the model Archaeon Halobacteriumsp. NRC-1: responses to changes in salinity and temperature in AQUATIC BIOSYSTEMS
  • 2013-01-16. Cloning, overexpression, purification, and characterization of a polyextremophilic β-galactosidase from the Antarctic haloarchaeon Halorubrum lacusprofundi in BMC BIOTECHNOLOGY
  • 2004-11-01. A common open representation of mass spectrometry data and its application to proteomics research in NATURE BIOTECHNOLOGY
  • 2005-07-04. UV irradiation induces homologous recombination genes in the model archaeon, Halobacterium sp. NRC-1 in AQUATIC BIOSYSTEMS
  • 2012-01-24. The core and unique proteins of haloarchaea in BMC GENOMICS
  • 2001-10. Genomic perspective on the photobiology of Halobacterium species NRC-1, a phototrophic, phototactic, and UV-tolerant haloarchaeon in PHOTOSYNTHESIS RESEARCH
  • 2008-08-29. Transcriptional responses to biologically relevant doses of UV-B radiation in the model archaeon, Halobacteriumsp. NRC-1 in AQUATIC BIOSYSTEMS
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    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/24292079


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