Microbial community structure of a pilot-scale thermophilic anaerobic digester treating poultry litter View Full Text


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Article Info

DATE

2013-08-30

AUTHORS

Ami M. Smith, Deepak Sharma, Hilary Lappin-Scott, Sara Burton, David H. Huber

ABSTRACT

The microbial community structure of a stable pilot-scale thermophilic continuous stirred tank reactor digester stabilized on poultry litter was investigated. This 40-m3 digester produced biogas with 57 % methane, and chemical oxygen demand removal of 54 %. Bacterial and archaeal diversity were examined using both cloning and pyrosequencing that targeted 16S rRNA genes. The bacterial community was dominated by phylum Firmicutes, constituting 93 % of the clones and 76 % of the pyrotags. Of the Firmicutes, class Clostridia (52 % pyrotags) was most abundant followed by class Bacilli (13 % pyrotags). The bacterial libraries identified 94 operational taxonomic units (OTUs) and pyrosequencing identified 577 OTUs at the 97 % minimum similarity level. Fifteen OTUs were dominant (≥2 % abundance), and nine of these were novel unclassified Firmicutes. Several of the dominant OTUs could not be classified more specifically than Clostridiales, but were most similar to plant biomass degraders, including Clostridium thermocellum. Of the rare pyrotag OTUs (<0.5 % abundance), 75 % were Firmicutes. The dominant methanogen was Methanothermobacter which has hydrogenotrophic metabolism, and accounted for >99 % of the archaeal clones. Based on the primary methanogen, as well as digester chemistry (high VA and ammonia levels), we propose that bacterial acetate oxidation is the primary pathway in this digester for the control of acetate levels. More... »

PAGES

2321-2334

References to SciGraph publications

  • 2006. Syntrophism among Prokaryotes in THE PROKARYOTES
  • 2006. The Family Lachnospiraceae, Including the Genera Butyrivibrio, Lachnospira and Roseburia in THE PROKARYOTES
  • 2006-03-20. Using pyrosequencing to shed light on deep mine microbial ecology in BMC GENOMICS
  • 1993-01. Thermophilic anaerobic digestion of livestock waste: the effect of ammonia in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2009-02-26. Towards the definition of a core of microorganisms involved in anaerobic digestion of sludge in THE ISME JOURNAL: MULTIDISCIPLINARY JOURNAL OF MICROBIAL ECOLOGY
  • 2010-06-07. The ecological coherence of high bacterial taxonomic ranks in NATURE REVIEWS MICROBIOLOGY
  • 2006-03. Macroscopic mass and energy balance of a pilot plant anaerobic bioreactor operated under thermophilic conditions in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 2009-07-14. Investigation of factors influencing biogas production in a large-scale thermophilic municipal biogas plant in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2007-03-03. Microbial population in the biomass adhering to supporting material in a packed-bed reactor degrading organic solid waste in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2007-04. Thermophilic anaerobic digester performance under different feed-loading frequency in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 2009-01-06. Effect of Heating Strategy on Power Consumption and Performance of a Pilot Plant Anaerobic Digester in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 2011-07-26. Characteristic microbial community of a dry thermophilic methanogenic digester: its long-term stability and change with feeding in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2006. Syntrophic Associations in Methanogenic Degradation in MOLECULAR BASIS OF SYMBIOSIS
  • 2008-11-01. Diversity of the resident microbiota in a thermophilic municipal biogas plant in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2013-01-10. Microdiversity of extracellular enzyme genes among sequenced prokaryotic genomes in THE ISME JOURNAL: MULTIDISCIPLINARY JOURNAL OF MICROBIAL ECOLOGY
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    http://scigraph.springernature.com/pub.10.1007/s00253-013-5144-y

    DOI

    http://dx.doi.org/10.1007/s00253-013-5144-y

    DIMENSIONS

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    PUBMED

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    43 schema:description The microbial community structure of a stable pilot-scale thermophilic continuous stirred tank reactor digester stabilized on poultry litter was investigated. This 40-m3 digester produced biogas with 57 % methane, and chemical oxygen demand removal of 54 %. Bacterial and archaeal diversity were examined using both cloning and pyrosequencing that targeted 16S rRNA genes. The bacterial community was dominated by phylum Firmicutes, constituting 93 % of the clones and 76 % of the pyrotags. Of the Firmicutes, class Clostridia (52 % pyrotags) was most abundant followed by class Bacilli (13 % pyrotags). The bacterial libraries identified 94 operational taxonomic units (OTUs) and pyrosequencing identified 577 OTUs at the 97 % minimum similarity level. Fifteen OTUs were dominant (≥2 % abundance), and nine of these were novel unclassified Firmicutes. Several of the dominant OTUs could not be classified more specifically than Clostridiales, but were most similar to plant biomass degraders, including Clostridium thermocellum. Of the rare pyrotag OTUs (<0.5 % abundance), 75 % were Firmicutes. The dominant methanogen was Methanothermobacter which has hydrogenotrophic metabolism, and accounted for >99 % of the archaeal clones. Based on the primary methanogen, as well as digester chemistry (high VA and ammonia levels), we propose that bacterial acetate oxidation is the primary pathway in this digester for the control of acetate levels.
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