Biodegradable plastic-degrading enzyme from Pseudozyma antarctica: cloning, sequencing, and characterization View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2013-04

AUTHORS

Yukiko Shinozaki, Tomotake Morita, Xiao-hong Cao, Shigenobu Yoshida, Motoo Koitabashi, Takashi Watanabe, Ken Suzuki, Yuka Sameshima-Yamashita, Toshiaki Nakajima-Kambe, Takeshi Fujii, Hiroko K. Kitamoto

ABSTRACT

Pseudozyma antarctica JCM 10317 exhibits a strong degradation activity for biodegradable plastics (BPs) such as agricultural mulch films composed of poly(butylene succinate) (PBS) and poly(butylene succinate-co-adipate) (PBSA). An enzyme named PaE was isolated and the gene encoding PaE was cloned from the strain by functional complementation in Saccharomyces cerevisiae. The deduced amino acid sequence of PaE contains 198 amino acids with a predicted molecular weight of 20,362.41. High identity was observed between this sequence and that of cutinase-like enzymes (CLEs) (61-68%); therefore, the gene encoding PaE was named PaCLE1. The specific activity of PaE against emulsified PBSA was 54.8±6.3 U/mg. In addition to emulsified BPs, PaE degraded solid films of PBS, PBSA, poly(ε-caprolactone), and poly(lactic acid). More... »

PAGES

2951-2959

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00253-012-4188-8

DOI

http://dx.doi.org/10.1007/s00253-012-4188-8

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1001579297

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/22678026


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Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00253-012-4188-8'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00253-012-4188-8'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00253-012-4188-8'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00253-012-4188-8'


 

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