A robust and extracellular heme-containing peroxidase from Thermobifida fusca as prototype of a bacterial peroxidase superfamily View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2010-05

AUTHORS

Edwin van Bloois, Daniel E. Torres Pazmiño, Remko T. Winter, Marco W. Fraaije

ABSTRACT

DyP-type peroxidases comprise a novel superfamily of heme-containing peroxidases which is unrelated to the superfamilies of known peroxidases and of which only a few members have been characterized in some detail. Here, we report the identification and characterization of a DyP-type peroxidase (TfuDyP) from the thermophilic actinomycete Thermobifida fusca. Biochemical characterization of the recombinant enzyme showed that it is a monomeric, heme-containing, thermostable, and Tat-dependently exported peroxidase. TfuDyP is not only active as dye-decolorizing peroxidase as it also accepts phenolic compounds and aromatic sulfides. In fact, it is able to catalyze enantioselective sulfoxidations, a type of reaction that has not been reported before for DyP-type peroxidases. Site-directed mutagenesis was used to determine the role of two conserved residues. D242 is crucial for catalysis while H338 represents the proximal heme ligand and is essential for heme incorporation. A genome database analysis revealed that DyP-type peroxidases are frequently found in bacterial genomes while they are extremely rare in other organisms. Most of the bacterial homologs are potential cytosolic enzymes, suggesting metabolic roles different from dye degradation. In conclusion, the detailed biochemical characterization reported here contributes significantly to our understanding of these enzymes and further emphasizes their biotechnological potential. More... »

PAGES

1419-1430

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00253-009-2369-x

DOI

http://dx.doi.org/10.1007/s00253-009-2369-x

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1029856227

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/19967355


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Actinomycetales", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Motifs", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Catalysis", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cloning, Molecular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Escherichia coli", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Heme", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Periplasm", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Peroxidases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Recombinant Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Alignment", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Stereoisomerism", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Substrate Specificity", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Groningen", 
          "id": "https://www.grid.ac/institutes/grid.4830.f", 
          "name": [
            "Laboratory of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747, Groningen, AG, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "van Bloois", 
        "givenName": "Edwin", 
        "id": "sg:person.0605350003.99", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0605350003.99"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Groningen", 
          "id": "https://www.grid.ac/institutes/grid.4830.f", 
          "name": [
            "Laboratory of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747, Groningen, AG, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Torres Pazmi\u00f1o", 
        "givenName": "Daniel E.", 
        "id": "sg:person.01174262675.35", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01174262675.35"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Groningen", 
          "id": "https://www.grid.ac/institutes/grid.4830.f", 
          "name": [
            "Laboratory of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747, Groningen, AG, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Winter", 
        "givenName": "Remko T.", 
        "id": "sg:person.01214525503.70", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01214525503.70"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Groningen", 
          "id": "https://www.grid.ac/institutes/grid.4830.f", 
          "name": [
            "Laboratory of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747, Groningen, AG, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Fraaije", 
        "givenName": "Marco W.", 
        "id": "sg:person.0652316424.03", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0652316424.03"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1042/bst0200337", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000753273"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1042/bst0200337", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000753273"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-006-0811-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002968310", 
          "https://doi.org/10.1007/s00253-006-0811-x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-006-0811-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002968310", 
          "https://doi.org/10.1007/s00253-006-0811-x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-2958.2004.04341.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003519243"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0003-2697(87)90643-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005157034"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/prot.21550", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006011255"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-004-1749-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006350539", 
          "https://doi.org/10.1007/s00253-004-1749-5"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0958-1669(00)00143-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006354740"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.phytochem.2007.04.005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006607701"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aem.02708-06", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007312636"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/bioinformatics/btm404", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007683223"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/b:phyt.0000047797.81958.69", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008343434", 
          "https://doi.org/10.1023/b:phyt.0000047797.81958.69"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0141-0229(86)90088-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012918522"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0141-0229(86)90088-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012918522"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.bbrc.2004.07.090", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013131084"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.micro.60.080805.142212", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014627325"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.273.29.18003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015595191"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-009-1904-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016148477", 
          "https://doi.org/10.1007/s00253-009-1904-0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0003-2697(83)90062-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016695591"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s002530100686", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017269711", 
          "https://doi.org/10.1007/s002530100686"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00299-005-0972-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018036906", 
          "https://doi.org/10.1007/s00299-005-0972-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00299-005-0972-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018036906", 
          "https://doi.org/10.1007/s00299-005-0972-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00299-005-0972-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018036906", 
          "https://doi.org/10.1007/s00299-005-0972-6"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0065-2164(05)58004-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019175898"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m706996200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021832899"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m511891200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022444827"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-2958.2001.02253.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024170854"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.phytochem.2003.10.022", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027388402"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.187.9.2983-2991.2005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029163669"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0958-1669(03)00074-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029409146"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0958-1669(03)00074-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029409146"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00018-008-8651-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029599907", 
          "https://doi.org/10.1007/s00018-008-8651-8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1179/135100000101535735", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030859275"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s00253-003-1236-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031966283", 
          "https://doi.org/10.1007/s00253-003-1236-4"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-2836(80)90283-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038788716"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10969-005-1103-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040567833", 
          "https://doi.org/10.1007/s10969-005-1103-x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s10969-005-1103-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040567833", 
          "https://doi.org/10.1007/s10969-005-1103-x"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1432-1327.1999.00685.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040674941"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0168-1656(97)01677-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040781443"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-2836(76)90119-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042857788"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/prot.21673", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044155176"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1471-2105-6-167", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044447875", 
          "https://doi.org/10.1186/1471-2105-6-167"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1471-2105-6-167", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044447875", 
          "https://doi.org/10.1186/1471-2105-6-167"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.182.7.1903-1909.2000", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045153617"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aem.66.4.1754-1758.2000", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047019410"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1186/1471-2148-8-101", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048110801", 
          "https://doi.org/10.1186/1471-2148-8-101"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkm960", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050102057"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/gkm960", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050102057"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ab.2005.04.017", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053461576"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi011869c", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055194540"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi011869c", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055194540"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi702296k", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055208153"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi702296k", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055208153"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1089/ars.2007.1927", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1059230742"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.2174/138920306776359786", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1069179116"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074256729", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077195804", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2010-05", 
    "datePublishedReg": "2010-05-01", 
    "description": "DyP-type peroxidases comprise a novel superfamily of heme-containing peroxidases which is unrelated to the superfamilies of known peroxidases and of which only a few members have been characterized in some detail. Here, we report the identification and characterization of a DyP-type peroxidase (TfuDyP) from the thermophilic actinomycete Thermobifida fusca. Biochemical characterization of the recombinant enzyme showed that it is a monomeric, heme-containing, thermostable, and Tat-dependently exported peroxidase. TfuDyP is not only active as dye-decolorizing peroxidase as it also accepts phenolic compounds and aromatic sulfides. In fact, it is able to catalyze enantioselective sulfoxidations, a type of reaction that has not been reported before for DyP-type peroxidases. Site-directed mutagenesis was used to determine the role of two conserved residues. D242 is crucial for catalysis while H338 represents the proximal heme ligand and is essential for heme incorporation. A genome database analysis revealed that DyP-type peroxidases are frequently found in bacterial genomes while they are extremely rare in other organisms. Most of the bacterial homologs are potential cytosolic enzymes, suggesting metabolic roles different from dye degradation. In conclusion, the detailed biochemical characterization reported here contributes significantly to our understanding of these enzymes and further emphasizes their biotechnological potential.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s00253-009-2369-x", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": true, 
    "isPartOf": [
      {
        "id": "sg:journal.1083533", 
        "issn": [
          "0175-7598", 
          "1432-0614"
        ], 
        "name": "Applied Microbiology and Biotechnology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "5", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "86"
      }
    ], 
    "name": "A robust and extracellular heme-containing peroxidase from Thermobifida fusca as prototype of a bacterial peroxidase superfamily", 
    "pagination": "1419-1430", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "8e3b7350dbf3190856917df051db6b687ea50a91399e733b93416ac77b883f13"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "19967355"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "8406612"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00253-009-2369-x"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1029856227"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00253-009-2369-x", 
      "https://app.dimensions.ai/details/publication/pub.1029856227"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T09:31", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000346_0000000346/records_99803_00000002.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/s00253-009-2369-x"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00253-009-2369-x'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00253-009-2369-x'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00253-009-2369-x'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00253-009-2369-x'


 

This table displays all metadata directly associated to this object as RDF triples.

296 TRIPLES      21 PREDICATES      90 URIs      35 LITERALS      23 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00253-009-2369-x schema:about N103198835e1a4a0ba03b65d4f0a68fa1
2 N1445787b434e4b1f88b6e33ef9d494e8
3 N49f862ab02d8456a817450c273180b77
4 N5bd22334cabc4c31823d8acce17ba260
5 N68c5dff69686408b936621e388d03789
6 N6957df89cb184a78a7a78b7f3b2393f2
7 N6ade536c2e60470a9888a54bd30902ad
8 N6fd38aef1218479090407bf52f1fc7c5
9 N7933b181fcb643118a1e1cd891d5b1bd
10 N898400606f114b839e3e42350d3b4158
11 N8af775c194644f29beb9145421d9eb1a
12 N8f0e737d82b146cd8fbfead98e736a6b
13 N8f15f3f5e3254f7bbbe62954d0403471
14 Nf3528a3efac54e6288278cca53892c68
15 anzsrc-for:06
16 anzsrc-for:0601
17 schema:author N7980b420fb704f7b8c619491d294daea
18 schema:citation sg:pub.10.1007/s00018-008-8651-8
19 sg:pub.10.1007/s00253-003-1236-4
20 sg:pub.10.1007/s00253-004-1749-5
21 sg:pub.10.1007/s00253-006-0811-x
22 sg:pub.10.1007/s00253-009-1904-0
23 sg:pub.10.1007/s002530100686
24 sg:pub.10.1007/s00299-005-0972-6
25 sg:pub.10.1007/s10969-005-1103-x
26 sg:pub.10.1023/b:phyt.0000047797.81958.69
27 sg:pub.10.1186/1471-2105-6-167
28 sg:pub.10.1186/1471-2148-8-101
29 https://app.dimensions.ai/details/publication/pub.1074256729
30 https://app.dimensions.ai/details/publication/pub.1077195804
31 https://doi.org/10.1002/prot.21550
32 https://doi.org/10.1002/prot.21673
33 https://doi.org/10.1016/0003-2697(83)90062-3
34 https://doi.org/10.1016/0003-2697(87)90643-9
35 https://doi.org/10.1016/0022-2836(76)90119-4
36 https://doi.org/10.1016/0022-2836(80)90283-1
37 https://doi.org/10.1016/0141-0229(86)90088-8
38 https://doi.org/10.1016/j.ab.2005.04.017
39 https://doi.org/10.1016/j.bbrc.2004.07.090
40 https://doi.org/10.1016/j.phytochem.2003.10.022
41 https://doi.org/10.1016/j.phytochem.2007.04.005
42 https://doi.org/10.1016/s0065-2164(05)58004-3
43 https://doi.org/10.1016/s0168-1656(97)01677-5
44 https://doi.org/10.1016/s0958-1669(00)00143-9
45 https://doi.org/10.1016/s0958-1669(03)00074-0
46 https://doi.org/10.1021/bi011869c
47 https://doi.org/10.1021/bi702296k
48 https://doi.org/10.1042/bst0200337
49 https://doi.org/10.1046/j.1365-2958.2001.02253.x
50 https://doi.org/10.1046/j.1432-1327.1999.00685.x
51 https://doi.org/10.1074/jbc.273.29.18003
52 https://doi.org/10.1074/jbc.m511891200
53 https://doi.org/10.1074/jbc.m706996200
54 https://doi.org/10.1089/ars.2007.1927
55 https://doi.org/10.1093/bioinformatics/btm404
56 https://doi.org/10.1093/nar/gkm960
57 https://doi.org/10.1111/j.1365-2958.2004.04341.x
58 https://doi.org/10.1128/aem.02708-06
59 https://doi.org/10.1128/aem.66.4.1754-1758.2000
60 https://doi.org/10.1128/jb.182.7.1903-1909.2000
61 https://doi.org/10.1128/jb.187.9.2983-2991.2005
62 https://doi.org/10.1146/annurev.micro.60.080805.142212
63 https://doi.org/10.1179/135100000101535735
64 https://doi.org/10.2174/138920306776359786
65 schema:datePublished 2010-05
66 schema:datePublishedReg 2010-05-01
67 schema:description DyP-type peroxidases comprise a novel superfamily of heme-containing peroxidases which is unrelated to the superfamilies of known peroxidases and of which only a few members have been characterized in some detail. Here, we report the identification and characterization of a DyP-type peroxidase (TfuDyP) from the thermophilic actinomycete Thermobifida fusca. Biochemical characterization of the recombinant enzyme showed that it is a monomeric, heme-containing, thermostable, and Tat-dependently exported peroxidase. TfuDyP is not only active as dye-decolorizing peroxidase as it also accepts phenolic compounds and aromatic sulfides. In fact, it is able to catalyze enantioselective sulfoxidations, a type of reaction that has not been reported before for DyP-type peroxidases. Site-directed mutagenesis was used to determine the role of two conserved residues. D242 is crucial for catalysis while H338 represents the proximal heme ligand and is essential for heme incorporation. A genome database analysis revealed that DyP-type peroxidases are frequently found in bacterial genomes while they are extremely rare in other organisms. Most of the bacterial homologs are potential cytosolic enzymes, suggesting metabolic roles different from dye degradation. In conclusion, the detailed biochemical characterization reported here contributes significantly to our understanding of these enzymes and further emphasizes their biotechnological potential.
68 schema:genre research_article
69 schema:inLanguage en
70 schema:isAccessibleForFree true
71 schema:isPartOf N3ed9507bf1394ac190b56b29736e3a82
72 N6b375296679a4ee388c370807ee2f1e6
73 sg:journal.1083533
74 schema:name A robust and extracellular heme-containing peroxidase from Thermobifida fusca as prototype of a bacterial peroxidase superfamily
75 schema:pagination 1419-1430
76 schema:productId N03a203549b54403282310914b747e6ef
77 N23606e8235a84f8aaad1a65fa5e2b60e
78 N3c20371a12dc4da5a76d0b8cc2f82195
79 N45d370961d974a0c89d34d61b94d5d12
80 N52c23b346ae9484393082912ef02abdb
81 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029856227
82 https://doi.org/10.1007/s00253-009-2369-x
83 schema:sdDatePublished 2019-04-11T09:31
84 schema:sdLicense https://scigraph.springernature.com/explorer/license/
85 schema:sdPublisher Nf01eda9abc49418eb116c04ba69537d0
86 schema:url http://link.springer.com/10.1007/s00253-009-2369-x
87 sgo:license sg:explorer/license/
88 sgo:sdDataset articles
89 rdf:type schema:ScholarlyArticle
90 N03a203549b54403282310914b747e6ef schema:name readcube_id
91 schema:value 8e3b7350dbf3190856917df051db6b687ea50a91399e733b93416ac77b883f13
92 rdf:type schema:PropertyValue
93 N103198835e1a4a0ba03b65d4f0a68fa1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
94 schema:name Periplasm
95 rdf:type schema:DefinedTerm
96 N1445787b434e4b1f88b6e33ef9d494e8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
97 schema:name Catalysis
98 rdf:type schema:DefinedTerm
99 N1fc77b1c4d46473aac3ccb443a4ae99a rdf:first sg:person.01174262675.35
100 rdf:rest N56d1aab5a76c45b5bd4d1b2c92a00d56
101 N23606e8235a84f8aaad1a65fa5e2b60e schema:name pubmed_id
102 schema:value 19967355
103 rdf:type schema:PropertyValue
104 N3c20371a12dc4da5a76d0b8cc2f82195 schema:name dimensions_id
105 schema:value pub.1029856227
106 rdf:type schema:PropertyValue
107 N3ed9507bf1394ac190b56b29736e3a82 schema:issueNumber 5
108 rdf:type schema:PublicationIssue
109 N45d370961d974a0c89d34d61b94d5d12 schema:name nlm_unique_id
110 schema:value 8406612
111 rdf:type schema:PropertyValue
112 N49f862ab02d8456a817450c273180b77 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
113 schema:name Cloning, Molecular
114 rdf:type schema:DefinedTerm
115 N52c23b346ae9484393082912ef02abdb schema:name doi
116 schema:value 10.1007/s00253-009-2369-x
117 rdf:type schema:PropertyValue
118 N56d1aab5a76c45b5bd4d1b2c92a00d56 rdf:first sg:person.01214525503.70
119 rdf:rest Nd920849738f44553a2cba21c3a829055
120 N5bd22334cabc4c31823d8acce17ba260 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Actinomycetales
122 rdf:type schema:DefinedTerm
123 N68c5dff69686408b936621e388d03789 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Molecular Sequence Data
125 rdf:type schema:DefinedTerm
126 N6957df89cb184a78a7a78b7f3b2393f2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
127 schema:name Heme
128 rdf:type schema:DefinedTerm
129 N6ade536c2e60470a9888a54bd30902ad schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
130 schema:name Amino Acid Motifs
131 rdf:type schema:DefinedTerm
132 N6b375296679a4ee388c370807ee2f1e6 schema:volumeNumber 86
133 rdf:type schema:PublicationVolume
134 N6fd38aef1218479090407bf52f1fc7c5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
135 schema:name Escherichia coli
136 rdf:type schema:DefinedTerm
137 N7933b181fcb643118a1e1cd891d5b1bd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
138 schema:name Stereoisomerism
139 rdf:type schema:DefinedTerm
140 N7980b420fb704f7b8c619491d294daea rdf:first sg:person.0605350003.99
141 rdf:rest N1fc77b1c4d46473aac3ccb443a4ae99a
142 N898400606f114b839e3e42350d3b4158 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
143 schema:name Substrate Specificity
144 rdf:type schema:DefinedTerm
145 N8af775c194644f29beb9145421d9eb1a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
146 schema:name Peroxidases
147 rdf:type schema:DefinedTerm
148 N8f0e737d82b146cd8fbfead98e736a6b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
149 schema:name Sequence Alignment
150 rdf:type schema:DefinedTerm
151 N8f15f3f5e3254f7bbbe62954d0403471 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
152 schema:name Amino Acid Sequence
153 rdf:type schema:DefinedTerm
154 Nd920849738f44553a2cba21c3a829055 rdf:first sg:person.0652316424.03
155 rdf:rest rdf:nil
156 Nf01eda9abc49418eb116c04ba69537d0 schema:name Springer Nature - SN SciGraph project
157 rdf:type schema:Organization
158 Nf3528a3efac54e6288278cca53892c68 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
159 schema:name Recombinant Proteins
160 rdf:type schema:DefinedTerm
161 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
162 schema:name Biological Sciences
163 rdf:type schema:DefinedTerm
164 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
165 schema:name Biochemistry and Cell Biology
166 rdf:type schema:DefinedTerm
167 sg:journal.1083533 schema:issn 0175-7598
168 1432-0614
169 schema:name Applied Microbiology and Biotechnology
170 rdf:type schema:Periodical
171 sg:person.01174262675.35 schema:affiliation https://www.grid.ac/institutes/grid.4830.f
172 schema:familyName Torres Pazmiño
173 schema:givenName Daniel E.
174 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01174262675.35
175 rdf:type schema:Person
176 sg:person.01214525503.70 schema:affiliation https://www.grid.ac/institutes/grid.4830.f
177 schema:familyName Winter
178 schema:givenName Remko T.
179 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01214525503.70
180 rdf:type schema:Person
181 sg:person.0605350003.99 schema:affiliation https://www.grid.ac/institutes/grid.4830.f
182 schema:familyName van Bloois
183 schema:givenName Edwin
184 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0605350003.99
185 rdf:type schema:Person
186 sg:person.0652316424.03 schema:affiliation https://www.grid.ac/institutes/grid.4830.f
187 schema:familyName Fraaije
188 schema:givenName Marco W.
189 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0652316424.03
190 rdf:type schema:Person
191 sg:pub.10.1007/s00018-008-8651-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029599907
192 https://doi.org/10.1007/s00018-008-8651-8
193 rdf:type schema:CreativeWork
194 sg:pub.10.1007/s00253-003-1236-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031966283
195 https://doi.org/10.1007/s00253-003-1236-4
196 rdf:type schema:CreativeWork
197 sg:pub.10.1007/s00253-004-1749-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006350539
198 https://doi.org/10.1007/s00253-004-1749-5
199 rdf:type schema:CreativeWork
200 sg:pub.10.1007/s00253-006-0811-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1002968310
201 https://doi.org/10.1007/s00253-006-0811-x
202 rdf:type schema:CreativeWork
203 sg:pub.10.1007/s00253-009-1904-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016148477
204 https://doi.org/10.1007/s00253-009-1904-0
205 rdf:type schema:CreativeWork
206 sg:pub.10.1007/s002530100686 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017269711
207 https://doi.org/10.1007/s002530100686
208 rdf:type schema:CreativeWork
209 sg:pub.10.1007/s00299-005-0972-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018036906
210 https://doi.org/10.1007/s00299-005-0972-6
211 rdf:type schema:CreativeWork
212 sg:pub.10.1007/s10969-005-1103-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1040567833
213 https://doi.org/10.1007/s10969-005-1103-x
214 rdf:type schema:CreativeWork
215 sg:pub.10.1023/b:phyt.0000047797.81958.69 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008343434
216 https://doi.org/10.1023/b:phyt.0000047797.81958.69
217 rdf:type schema:CreativeWork
218 sg:pub.10.1186/1471-2105-6-167 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044447875
219 https://doi.org/10.1186/1471-2105-6-167
220 rdf:type schema:CreativeWork
221 sg:pub.10.1186/1471-2148-8-101 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048110801
222 https://doi.org/10.1186/1471-2148-8-101
223 rdf:type schema:CreativeWork
224 https://app.dimensions.ai/details/publication/pub.1074256729 schema:CreativeWork
225 https://app.dimensions.ai/details/publication/pub.1077195804 schema:CreativeWork
226 https://doi.org/10.1002/prot.21550 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006011255
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1002/prot.21673 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044155176
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1016/0003-2697(83)90062-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016695591
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1016/0003-2697(87)90643-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005157034
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1016/0022-2836(76)90119-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042857788
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1016/0022-2836(80)90283-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038788716
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1016/0141-0229(86)90088-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012918522
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1016/j.ab.2005.04.017 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053461576
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1016/j.bbrc.2004.07.090 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013131084
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1016/j.phytochem.2003.10.022 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027388402
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1016/j.phytochem.2007.04.005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006607701
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1016/s0065-2164(05)58004-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019175898
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1016/s0168-1656(97)01677-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040781443
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1016/s0958-1669(00)00143-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006354740
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1016/s0958-1669(03)00074-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029409146
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1021/bi011869c schema:sameAs https://app.dimensions.ai/details/publication/pub.1055194540
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1021/bi702296k schema:sameAs https://app.dimensions.ai/details/publication/pub.1055208153
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1042/bst0200337 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000753273
261 rdf:type schema:CreativeWork
262 https://doi.org/10.1046/j.1365-2958.2001.02253.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1024170854
263 rdf:type schema:CreativeWork
264 https://doi.org/10.1046/j.1432-1327.1999.00685.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1040674941
265 rdf:type schema:CreativeWork
266 https://doi.org/10.1074/jbc.273.29.18003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015595191
267 rdf:type schema:CreativeWork
268 https://doi.org/10.1074/jbc.m511891200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022444827
269 rdf:type schema:CreativeWork
270 https://doi.org/10.1074/jbc.m706996200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021832899
271 rdf:type schema:CreativeWork
272 https://doi.org/10.1089/ars.2007.1927 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059230742
273 rdf:type schema:CreativeWork
274 https://doi.org/10.1093/bioinformatics/btm404 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007683223
275 rdf:type schema:CreativeWork
276 https://doi.org/10.1093/nar/gkm960 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050102057
277 rdf:type schema:CreativeWork
278 https://doi.org/10.1111/j.1365-2958.2004.04341.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1003519243
279 rdf:type schema:CreativeWork
280 https://doi.org/10.1128/aem.02708-06 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007312636
281 rdf:type schema:CreativeWork
282 https://doi.org/10.1128/aem.66.4.1754-1758.2000 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047019410
283 rdf:type schema:CreativeWork
284 https://doi.org/10.1128/jb.182.7.1903-1909.2000 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045153617
285 rdf:type schema:CreativeWork
286 https://doi.org/10.1128/jb.187.9.2983-2991.2005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029163669
287 rdf:type schema:CreativeWork
288 https://doi.org/10.1146/annurev.micro.60.080805.142212 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014627325
289 rdf:type schema:CreativeWork
290 https://doi.org/10.1179/135100000101535735 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030859275
291 rdf:type schema:CreativeWork
292 https://doi.org/10.2174/138920306776359786 schema:sameAs https://app.dimensions.ai/details/publication/pub.1069179116
293 rdf:type schema:CreativeWork
294 https://www.grid.ac/institutes/grid.4830.f schema:alternateName University of Groningen
295 schema:name Laboratory of Biochemistry, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, 9747, Groningen, AG, The Netherlands
296 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...