Ontology type: schema:ScholarlyArticle
2009-06-25
AUTHORSAnna Malandra, Maria Cantarella, Ondřej Kaplan, Vojtěch Vejvoda, Bronislava Uhnáková, Barbora Štěpánková, David Kubáč, Ludmila Martínková
ABSTRACTThe operational stabilities of nitrilases from Aspergillus niger K10 and Fusarium solani O1 were examined with 4-cyanopyridine as the substrate in continuous-stirred membrane reactors (CSMRs). The former enzyme was fairly stable at 30 °C with a deactivation constant (kd) and enzyme half-life of 0.014 h−1 and 50 h, respectively, but the latter exhibited an even higher stability characterized by kd = 0.008 h−1 and half-life of 87 h at 40 °C. Another advantage of this enzyme was its high chemoselectivity, i.e., selective transformation of nitriles into carboxylic acids, while the amide formed a high ratio of A. niger K10 nitrilase product. High conversion rates (>90%) were maintained for about 52 h using the nitrilase from F. solani O1 immobilized in cross-linked enzyme aggregates (CLEAs). The purity of isonicotinic acid was increased from 98% to >99.9% by using two CSMRs connected in series, the first one containing the F. solani O1 nitrilase and the second the amidase from Rhodococcus erythropolis A4 (both enzymes as CLEAs), the amidase hydrolyzing the by-product isonicotinamide. More... »
PAGES277
http://scigraph.springernature.com/pub.10.1007/s00253-009-2073-x
DOIhttp://dx.doi.org/10.1007/s00253-009-2073-x
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1048531805
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/19554325
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biochemistry and Cell Biology",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Aminohydrolases",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Aspergillus niger",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Bioreactors",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Biotransformation",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Enzyme Stability",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Fusarium",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Isonicotinic Acids",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Kinetics",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Nitriles",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Pyridines",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Substrate Specificity",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Institute of Microbiology, Centre of Biocatalysis and Biotransformation, Academy of Sciences of the Czech Republic, V\u00edde\u0148sk\u00e1 1083, CZ-142 20, Prague, Czech Republic",
"id": "http://www.grid.ac/institutes/grid.418800.5",
"name": [
"Department of Chemistry, Chemical Engineering and Materials, University of L\u2019Aquila, Monteluco di Roio, 67040, L\u2019Aquila, Italy",
"Institute of Microbiology, Centre of Biocatalysis and Biotransformation, Academy of Sciences of the Czech Republic, V\u00edde\u0148sk\u00e1 1083, CZ-142 20, Prague, Czech Republic"
],
"type": "Organization"
},
"familyName": "Malandra",
"givenName": "Anna",
"id": "sg:person.0603442461.47",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0603442461.47"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Department of Chemistry, Chemical Engineering and Materials, University of L\u2019Aquila, Monteluco di Roio, 67040, L\u2019Aquila, Italy",
"id": "http://www.grid.ac/institutes/grid.158820.6",
"name": [
"Department of Chemistry, Chemical Engineering and Materials, University of L\u2019Aquila, Monteluco di Roio, 67040, L\u2019Aquila, Italy"
],
"type": "Organization"
},
"familyName": "Cantarella",
"givenName": "Maria",
"id": "sg:person.01257735016.51",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01257735016.51"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Institute of Microbiology, Centre of Biocatalysis and Biotransformation, Academy of Sciences of the Czech Republic, V\u00edde\u0148sk\u00e1 1083, CZ-142 20, Prague, Czech Republic",
"id": "http://www.grid.ac/institutes/grid.418800.5",
"name": [
"Institute of Microbiology, Centre of Biocatalysis and Biotransformation, Academy of Sciences of the Czech Republic, V\u00edde\u0148sk\u00e1 1083, CZ-142 20, Prague, Czech Republic"
],
"type": "Organization"
},
"familyName": "Kaplan",
"givenName": "Ond\u0159ej",
"id": "sg:person.01311100504.27",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01311100504.27"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Institute of Microbiology, Centre of Biocatalysis and Biotransformation, Academy of Sciences of the Czech Republic, V\u00edde\u0148sk\u00e1 1083, CZ-142 20, Prague, Czech Republic",
"id": "http://www.grid.ac/institutes/grid.418800.5",
"name": [
"Institute of Microbiology, Centre of Biocatalysis and Biotransformation, Academy of Sciences of the Czech Republic, V\u00edde\u0148sk\u00e1 1083, CZ-142 20, Prague, Czech Republic"
],
"type": "Organization"
},
"familyName": "Vejvoda",
"givenName": "Vojt\u011bch",
"id": "sg:person.01006545431.23",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01006545431.23"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Institute of Microbiology, Centre of Biocatalysis and Biotransformation, Academy of Sciences of the Czech Republic, V\u00edde\u0148sk\u00e1 1083, CZ-142 20, Prague, Czech Republic",
"id": "http://www.grid.ac/institutes/grid.418800.5",
"name": [
"Institute of Microbiology, Centre of Biocatalysis and Biotransformation, Academy of Sciences of the Czech Republic, V\u00edde\u0148sk\u00e1 1083, CZ-142 20, Prague, Czech Republic"
],
"type": "Organization"
},
"familyName": "Uhn\u00e1kov\u00e1",
"givenName": "Bronislava",
"id": "sg:person.01327046561.62",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01327046561.62"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Institute of Microbiology, Centre of Biocatalysis and Biotransformation, Academy of Sciences of the Czech Republic, V\u00edde\u0148sk\u00e1 1083, CZ-142 20, Prague, Czech Republic",
"id": "http://www.grid.ac/institutes/grid.418800.5",
"name": [
"Institute of Microbiology, Centre of Biocatalysis and Biotransformation, Academy of Sciences of the Czech Republic, V\u00edde\u0148sk\u00e1 1083, CZ-142 20, Prague, Czech Republic"
],
"type": "Organization"
},
"familyName": "\u0160t\u011bp\u00e1nkov\u00e1",
"givenName": "Barbora",
"type": "Person"
},
{
"affiliation": {
"alternateName": "Institute of Microbiology, Centre of Biocatalysis and Biotransformation, Academy of Sciences of the Czech Republic, V\u00edde\u0148sk\u00e1 1083, CZ-142 20, Prague, Czech Republic",
"id": "http://www.grid.ac/institutes/grid.418800.5",
"name": [
"Institute of Microbiology, Centre of Biocatalysis and Biotransformation, Academy of Sciences of the Czech Republic, V\u00edde\u0148sk\u00e1 1083, CZ-142 20, Prague, Czech Republic"
],
"type": "Organization"
},
"familyName": "Kub\u00e1\u010d",
"givenName": "David",
"id": "sg:person.015611352104.40",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015611352104.40"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Institute of Microbiology, Centre of Biocatalysis and Biotransformation, Academy of Sciences of the Czech Republic, V\u00edde\u0148sk\u00e1 1083, CZ-142 20, Prague, Czech Republic",
"id": "http://www.grid.ac/institutes/grid.418800.5",
"name": [
"Institute of Microbiology, Centre of Biocatalysis and Biotransformation, Academy of Sciences of the Czech Republic, V\u00edde\u0148sk\u00e1 1083, CZ-142 20, Prague, Czech Republic"
],
"type": "Organization"
},
"familyName": "Mart\u00ednkov\u00e1",
"givenName": "Ludmila",
"id": "sg:person.01127642146.08",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01127642146.08"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1007/s00253-002-1062-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1045415936",
"https://doi.org/10.1007/s00253-002-1062-0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s10295-006-0161-9",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1048535950",
"https://doi.org/10.1007/s10295-006-0161-9"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00253-006-0503-6",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044847715",
"https://doi.org/10.1007/s00253-006-0503-6"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00253-003-1495-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1049118464",
"https://doi.org/10.1007/s00253-003-1495-0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s11274-006-9230-5",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1001603605",
"https://doi.org/10.1007/s11274-006-9230-5"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s007920050129",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1050996276",
"https://doi.org/10.1007/s007920050129"
],
"type": "CreativeWork"
}
],
"datePublished": "2009-06-25",
"datePublishedReg": "2009-06-25",
"description": "The operational stabilities of nitrilases from Aspergillus niger K10 and Fusarium solani O1 were examined with 4-cyanopyridine as the substrate in continuous-stirred membrane reactors (CSMRs). The former enzyme was fairly stable at 30\u00a0\u00b0C with a deactivation constant (kd) and enzyme half-life of 0.014\u00a0h\u22121 and 50\u00a0h, respectively, but the latter exhibited an even higher stability characterized by kd\u2009=\u20090.008\u00a0h\u22121 and half-life of 87\u00a0h at 40\u00a0\u00b0C. Another advantage of this enzyme was its high chemoselectivity, i.e., selective transformation of nitriles into carboxylic acids, while the amide formed a high ratio of A. niger K10 nitrilase product. High conversion rates (>90%) were maintained for about 52\u00a0h using the nitrilase from F. solani O1 immobilized in cross-linked enzyme aggregates (CLEAs). The purity of isonicotinic acid was increased from 98% to >99.9% by using two CSMRs connected in series, the first one containing the F. solani O1 nitrilase and the second the amidase from Rhodococcus erythropolis A4 (both enzymes as CLEAs), the amidase hydrolyzing the by-product isonicotinamide.",
"genre": "article",
"id": "sg:pub.10.1007/s00253-009-2073-x",
"isAccessibleForFree": false,
"isFundedItemOf": [
{
"id": "sg:grant.6880876",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.6881768",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.6888850",
"type": "MonetaryGrant"
},
{
"id": "sg:grant.6882219",
"type": "MonetaryGrant"
}
],
"isPartOf": [
{
"id": "sg:journal.1083533",
"issn": [
"0175-7598",
"1432-0614"
],
"name": "Applied Microbiology and Biotechnology",
"publisher": "Springer Nature",
"type": "Periodical"
},
{
"issueNumber": "2",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "85"
}
],
"keywords": [
"former enzyme",
"Aspergillus niger K10",
"nitrilases",
"amidase",
"enzyme",
"Rhodococcus erythropolis A4",
"O1",
"nitrilase",
"acid",
"Kd",
"high ratio",
"hydrolysis",
"K10",
"substrate",
"enzyme aggregates",
"A4",
"continuous hydrolysis",
"aggregates",
"cross-linked enzyme aggregates",
"carboxylic acids",
"stability",
"products",
"amides",
"transformation",
"high stability",
"isonicotinic acid",
"high conversion rate",
"deactivation constant",
"rate",
"conversion rate",
"purity",
"isonicotinamide",
"one",
"advantages",
"ratio",
"series",
"nitriles",
"first one",
"constants",
"selective transformation",
"high chemoselectivity",
"membrane reactor",
"operational stability",
"reactor",
"chemoselectivity"
],
"name": "Continuous hydrolysis of 4-cyanopyridine by nitrilases from Fusarium solani O1 and Aspergillus niger K10",
"pagination": "277",
"productId": [
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1048531805"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/s00253-009-2073-x"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"19554325"
]
}
],
"sameAs": [
"https://doi.org/10.1007/s00253-009-2073-x",
"https://app.dimensions.ai/details/publication/pub.1048531805"
],
"sdDataset": "articles",
"sdDatePublished": "2022-08-04T16:58",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-springernature-scigraph/baseset/20220804/entities/gbq_results/article/article_490.jsonl",
"type": "ScholarlyArticle",
"url": "https://doi.org/10.1007/s00253-009-2073-x"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00253-009-2073-x'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00253-009-2073-x'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00253-009-2073-x'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00253-009-2073-x'
This table displays all metadata directly associated to this object as RDF triples.
234 TRIPLES
21 PREDICATES
87 URIs
73 LITERALS
18 BLANK NODES