Continuous hydrolysis of 4-cyanopyridine by nitrilases from Fusarium solani O1 and Aspergillus niger K10 View Full Text


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Article Info

DATE

2009-06-25

AUTHORS

Anna Malandra, Maria Cantarella, Ondřej Kaplan, Vojtěch Vejvoda, Bronislava Uhnáková, Barbora Štěpánková, David Kubáč, Ludmila Martínková

ABSTRACT

The operational stabilities of nitrilases from Aspergillus niger K10 and Fusarium solani O1 were examined with 4-cyanopyridine as the substrate in continuous-stirred membrane reactors (CSMRs). The former enzyme was fairly stable at 30 °C with a deactivation constant (kd) and enzyme half-life of 0.014 h−1 and 50 h, respectively, but the latter exhibited an even higher stability characterized by kd = 0.008 h−1 and half-life of 87 h at 40 °C. Another advantage of this enzyme was its high chemoselectivity, i.e., selective transformation of nitriles into carboxylic acids, while the amide formed a high ratio of A. niger K10 nitrilase product. High conversion rates (>90%) were maintained for about 52 h using the nitrilase from F. solani O1 immobilized in cross-linked enzyme aggregates (CLEAs). The purity of isonicotinic acid was increased from 98% to >99.9% by using two CSMRs connected in series, the first one containing the F. solani O1 nitrilase and the second the amidase from Rhodococcus erythropolis A4 (both enzymes as CLEAs), the amidase hydrolyzing the by-product isonicotinamide. More... »

PAGES

277

References to SciGraph publications

  • 2006-07-20. A propionitrile-induced nitrilase of Rhodococcus sp. NDB 1165 and its application in nicotinic acid synthesis in WORLD JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
  • 2002-10-01. RETRACTED ARTICLE: The nitrile-degrading enzymes: current status and future prospects in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2006-08-15. Hyperinduction of nitrilases in filamentous fungi in JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
  • 1999-11. Characterization of an inducible nitrilase from a thermophilic bacillus in EXTREMOPHILES
  • 2003-12-10. Characterisation of nitrilase and nitrile hydratase biocatalytic systems in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2006-10-24. Purification and characterization of a nitrilase from Aspergillus niger K10 in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00253-009-2073-x

    DOI

    http://dx.doi.org/10.1007/s00253-009-2073-x

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1048531805

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/19554325


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