Ontology type: schema:ScholarlyArticle
2009-06-25
AUTHORSAnna Malandra, Maria Cantarella, Ondřej Kaplan, Vojtěch Vejvoda, Bronislava Uhnáková, Barbora Štěpánková, David Kubáč, Ludmila Martínková
ABSTRACTThe operational stabilities of nitrilases from Aspergillus niger K10 and Fusarium solani O1 were examined with 4-cyanopyridine as the substrate in continuous-stirred membrane reactors (CSMRs). The former enzyme was fairly stable at 30 °C with a deactivation constant (kd) and enzyme half-life of 0.014 h−1 and 50 h, respectively, but the latter exhibited an even higher stability characterized by kd = 0.008 h−1 and half-life of 87 h at 40 °C. Another advantage of this enzyme was its high chemoselectivity, i.e., selective transformation of nitriles into carboxylic acids, while the amide formed a high ratio of A. niger K10 nitrilase product. High conversion rates (>90%) were maintained for about 52 h using the nitrilase from F. solani O1 immobilized in cross-linked enzyme aggregates (CLEAs). The purity of isonicotinic acid was increased from 98% to >99.9% by using two CSMRs connected in series, the first one containing the F. solani O1 nitrilase and the second the amidase from Rhodococcus erythropolis A4 (both enzymes as CLEAs), the amidase hydrolyzing the by-product isonicotinamide. More... »
PAGES277
http://scigraph.springernature.com/pub.10.1007/s00253-009-2073-x
DOIhttp://dx.doi.org/10.1007/s00253-009-2073-x
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PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/19554325
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