Cloning and functional characterization of the cis-aconitic acid decarboxylase (CAD) gene from Aspergillus terreus View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2008-08

AUTHORS

Shin Kanamasa, Lies Dwiarti, Mitsuyasu Okabe, Enoch Y. Park

ABSTRACT

A filamentous fungus Aspergillus terreus produces itaconic acid, which is predicted to be derived from cis-aconitic acid via catalysis by cis-aconitic acid decarboxylase (CAD) in the carbon metabolism of the fungus. To clarify the enzyme's function and a pathway for itaconic acid biosynthesis, we cloned a novel gene encoding the enzyme. The open reading frame of this gene (CAD1) consists of 1,529 bp encoding 490 amino acids and is interrupted by a single intron. Among the identified proteins in the database, the primary structure of the protein encoded by CAD1 shared high identity with the MmgE/PrpD family of proteins, including a number of 2-methylcitrate dehydratases of bacteria. The cloned gene excluding an intron was introduced into the expression plasmid pAUR-CAD1 controlled by the ADH1 promoter. The CAD activity in Saccharomyces cerevisiae was confirmed by directly detecting itaconic acid as a product from cis-aconitic acid as a substrate. This result reveals for the first time that this gene encodes CAD, which is essential for itaconic acid production in A. terreus. More... »

PAGES

223-229

Journal

TITLE

Applied Microbiology and Biotechnology

ISSUE

2

VOLUME

80

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00253-008-1523-1

    DOI

    http://dx.doi.org/10.1007/s00253-008-1523-1

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1028873842

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/18584171


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