Molecular cloning and heterologous expression of a laccase gene from Pleurotus eryngii in free and immobilized Saccharomyces cerevisiae cells View Full Text


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Article Info

DATE

2008-07

AUTHORS

Gianluca Bleve, Chiara Lezzi, Giovanni Mita, Patrizia Rampino, Carla Perrotta, Luciano Villanova, Francesco Grieco

ABSTRACT

A full length cDNA encoding an extracellular laccase was isolated by reverse transcription polymerase chain reaction from the mycelia of the mushroom Pleurotus eryngii. The isolated sequence, denoted Ery3, encodes for a mature laccase isoenzyme of 531 amino acid residues with a predicted molecular weight of 56.6 kDa. All sequence motifs, being the signature sequences used to identify the laccases, were found in the Ery3 protein sequence. The Ery3 cDNA was expressed in Saccharomyces cerevisiae and the effects of copper concentration and cultivation temperature were investigated. S. cerevisiae cells were immobilized in calcium alginate gel and the optimal immobilization parameters for the enhanced production of laccase were determined. The immobilization was most effective with 3% sodium alginate, 0.1 M calcium chloride and an initial biomass of 4.5 x 10(8) cells. The enzyme yield obtained with immobilized cells (139 mU ml(-1)) showed a 1.6-fold increase compared to the highest yield obtained with free cells. The alginate beads showed good stability and retained 84% capacity of enzyme production after seven repeated cycles of batch fermentation. The immobilization system proved to increase the proteolytic stability of the recombinant Ery3 protein. To our knowledge, this is the first report on S. cerevisiae whole-cell immobilization for recombinant laccase production. More... »

PAGES

731

References to SciGraph publications

  • 1990-07. Use of a Cell Recycle Reactor to Increase Production of a Proteolysis-Susceptible Peptide Secreted from Recombinant Saccharomyces cerevisiae in BIO/TECHNOLOGY
  • 1995-06. Chemical and cytological changes during the autolysis of yeasts in JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
  • 2000-05. Proteolytic stability of recombinant human serum albumin secreted in the yeast Saccharomyces cerevisiae in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2005-01. Expression of laccase IIIb from the white-rot fungus Trametes versicolor in the yeast Yarrowia lipolytica for environmental applications in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 1990-10. Trehalose in yeast, stress protectant rather than reserve carbohydrate in ANTONIE VAN LEEUWENHOEK
  • 2006-10. Immobilized yeast cell systems for continuous fermentation applications in BIOTECHNOLOGY LETTERS
  • 1997-01. Induction and Characterization of Laccase in the Ligninolytic Fungus Pleurotus eryngii in CURRENT MICROBIOLOGY
  • 1998-06. Inhibition of extracellular protease secretion by Aspergillus niger using cell immobilization in BIOTECHNOLOGY LETTERS
  • 2006-01. Purification of a laccase from fruiting bodies of the mushroom Pleurotus eryngii in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 1989-05. Effects of immobilization on growth, substrate consumption, β-galactosidase induction, and byproduct formation inEscherichia coli in JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
  • 1998-04. Crystal structure of the type-2 Cu depleted laccase from Coprinus cinereus at 2.2 Å resolution in NATURE STRUCTURAL & MOLECULAR BIOLOGY
  • 2006-06. Expression of laccase gene lcc1 in Coprinopsis cinerea under control of various basidiomycetous promoters in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 1999-09. Characterization of a gene encoding Trametes versicolor laccase A and improved heterologous expression in Saccharomyces cerevisiae by decreased cultivation temperature in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2005-11. Molecular cloning of the cDNA encoding laccase from Trametes versicolor and heterologous expression in Pichia methanolica in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2005-12. Recombinant expression of Pleurotus ostreatus laccases in Kluyveromyces lactis and Saccharomyces cerevisiae in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 1993-07. Stability and activity of a phenol oxidase from the ligninolytic fungus Pleurotus ostreatus in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2002-04. Biotechnological applications and potential of wood-degrading mushrooms of the genus Pleurotus in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00253-008-1479-1

    DOI

    http://dx.doi.org/10.1007/s00253-008-1479-1

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1026047022

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/18443781


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