Biosynthesis of enantiopure (S)-3-hydroxybutyric acid in metabolically engineered Escherichia coli View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2008-06

AUTHORS

Sang-Hyun Lee, Si Jae Park, Sang Yup Lee, Soon Ho Hong

ABSTRACT

A biosynthetic pathway for the production of (S)-3-hydroxybutyric acid (S3HB) from glucose was established in recombinant Escherichia coli by introducing the beta-ketothiolase gene from Ralstonia eutropha H16, the (S)-3-hydroxybutyryl-CoA dehydrogenase gene from R. eutropha H16, or Clostridium acetobutylicum ATCC824, and the 3-hydroxyisobutyryl-CoA hydrolase gene from Bacillus cereus ATCC14579. Artificial operon consisting of these genes was constructed and was expressed in E. coli BL21 (DE3) codon plus under T7 promoter by isopropyl beta-D: -thiogalactoside (IPTG) induction. Recombinant E. coli BL21 (DE3) codon plus expressing the beta-ketothiolase gene, the (S)-3-hydroxybutyryl-CoA dehydrogenase gene, and the 3-hydroxyisobutyryl-CoA hydrolase gene could synthesize enantiomerically pure S3HB to the concentration of 0.61 g l(-1) from 20 g l(-1) of glucose in Luria-Bertani medium. Fed-batch cultures of recombinant E. coli BL21 (DE3) codon plus were carried out to achieve higher titer of S3HB with varying induction time and glucose concentration during fermentation. Protein expression was induced by addition of 1 mM IPTG when cell concentration reached 10 and 20 g l(-1) (OD(600) = 30 and 60), respectively. When protein expression was induced at 60 of OD(600) and glucose was fed to the concentration of 15 g l(-1), 10.3 g l(-1) of S3HB was obtained in 38 h with the S3HB productivity of 0.21 g l(-1)h(-1). Lowering glucose concentration to 5 g l(-1) and induction of protein expression at 30 of OD(600) significantly reduced final S3HB concentration to 3.7 g l(-1), which also resulted in the decrease of the S3HB productivity to 0.05 g l(-1)h(-1). More... »

PAGES

633-641

References to SciGraph publications

Journal

TITLE

Applied Microbiology and Biotechnology

ISSUE

4

VOLUME

79

Related Patents

  • Microorganisms For The Production Of 1,4-Butanediol
  • Microorganisms And Methods For The Co-Production Of Isopropanol And 1,4-Butanediol
  • Process And Systems For Obtaining 1,4-Butanediol From Fermentation Broths
  • Microorganisms And Methods For The Coproduction 1,4-Butanediol And Gamma-Butyrolactone
  • Microorganisms For The Production Of Methacrylic Acid
  • Microorganisms And Methods For Production Of 4-Hydroxybutyrate, 1,4-Butanediol And Related Compounds
  • Microorganisms And Methods For Conversion Of Syngas And Other Carbon Sources To Useful Products
  • Methods And Organisms For Converting Synthesis Gas Or Other Gaseous Carbon Sources And Methanol To 1,3-Butanediol
  • Semi-Synthetic Terephthalic Acid Via Microorganisms That Produce Muconic Acid
  • Methods And Organisms For Converting Synthesis Gas Or Other Gaseous Carbon Sources And Methanol To 1,3-Butanediol
  • Compositions And Methods For The Biosynthesis Of 1,4-Butanediol And Its Precursors
  • Microorganisms For The Production Of Adipic Acid And Other Compounds
  • Methods And Organisms For Utilizing Synthesis Gas Or Other Gaseous Carbon Sources And Methanol
  • Microorganisms And Methods For The Biosynthesis Of Butadiene
  • Microorganisms For The Production Of 1,4-Butanediol
  • Microorganisms And Methods For The Biosynthesis Of Propylene
  • Semi-Synthetic Terephthalic Acid Via Microorganisms That Produce Muconic Acid
  • Microorganisms And Methods For The Biosynthesis Of Adipate, Hexamethylenediamine And 6-Aminocaproic Acid
  • Microbial Production Of 3-Hydroxyacids From Glucose And Glycolate
  • Process Of Separating Components Of A Fermentation Broth
  • Methods For The Synthesis Of Olefins And Derivatives
  • Microorganisms And Methods For Carbon-Efficient Biosynthesis Of Mek And 2-Butanol
  • Microorganisms For The Production Of 1,4-Butanediol And Related Methods
  • Microorganisms For The Production Of 2-Hydroxyisobutyric Acid
  • Microorganisms For The Production Of Methacrylic Acid
  • Microorganisms And Methods For Conversion Of Syngas And Other Carbon Sources To Useful Products
  • Process Of Separating Components Of A Fermentation Broth
  • Microorganisms And Methods For The Coproduction 1,4-Butanediol And Gamma-Butyrolactone
  • Microorganisms And Methods For The Biosynthesis Of Fumarate, Malate, And Acrylate
  • Methods And Organisms For Utilizing Synthesis Gas Or Other Gaseous Carbon Sources And Methanol
  • Microorganisms And Methods For The Coproduction 1,4-Butanediol And Gamma-Butyrolactone
  • Microorganisms And Methods For The Biosynthesis Of Aromatics, 2,4-Pentadienoate And 1,3-Butadiene
  • Microorganisms And Methods For Conversion Of Syngas And Other Carbon Sources To Useful Products
  • Organisms For The Production Of 1,3-Butanediol
  • Microorganisms For The Production Of Methacrylic Acid
  • Microorganisms For The Production Of 1,4-Butanediol
  • Microbial Organisms Comprising Exogenous Nucleic Acids Encoding Reductive Tca Pathway Enzymes
  • Preparing Method For (S)-3hydroxybutyric Acid And (S)-3 Hydroxybutyrate Ester Using Recombinant Microorganism
  • Microorganisms For The Production Of 1,4-Butanediol, 4-Hydroxybutanal, 4-Hydroxybutyryl-Coa, Putrescine And Related Compounds, And Methods Related Thereto
  • Methods And Organisms For The Growth-Coupled Production Of 1,4-Butanediol
  • Microorganisms For The Production Of Adipic Acid And Other Compounds
  • Methods And Organisms For Utilizing Synthesis Gas Or Other Gaseous Carbon Sources And Methanol
  • Methods For Increasing Product Yields
  • Microorganisms For The Production Of 1,4-Butanediol, 4-Hydroxybutanal, 4-Hydroxybutyryl-Coa, Putrescine And Related Compounds, And Methods Related Thereto
  • Methods And Organisms For Utilizing Synthesis Gas Or Other Gaseous Carbon Sources And Methanol
  • Microorganisms For The Production Of Adipic Acid And Other Compounds
  • Primary Alcohol Producing Organisms
  • Primary Alcohol Producing Organisms
  • Methods And Organisms For Utilizing Synthesis Gas Or Other Gaseous Carbon Sources And Methanol
  • Methods And Organisms For The Growth-Coupled Production Of 1,4-Butanediol
  • Organisms For The Production Of Isopropanol, N-Butanol, And Isobutanol
  • Microorganisms For The Production Of 1,4-Butanediol
  • Microorganisms For The Production Of 1,4-Butanediol And Related Methods
  • Methods And Organisms For Utilizing Synthesis Gas Or Other Gaseous Carbon Sources And Methanol
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00253-008-1473-7

    DOI

    http://dx.doi.org/10.1007/s00253-008-1473-7

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1026112726

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/18461320


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "3-Hydroxyacyl CoA Dehydrogenases", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "3-Hydroxybutyric Acid", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Acetyl-CoA C-Acyltransferase", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Bacillus cereus", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Bacterial Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Biomass", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Bioreactors", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Biosynthetic Pathways", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Clostridium acetobutylicum", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Cupriavidus necator", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Escherichia coli", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genetic Engineering", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Glucose", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Industrial Microbiology", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Thiolester Hydrolases", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "LG Corporation (South Korea)", 
              "id": "https://www.grid.ac/institutes/grid.464630.3", 
              "name": [
                "Corporate R&D, LG Chem, Ltd./Research Park, 104-1 Moonji-dong, Yuseong-gu, 305-380, Daejeon, Republic of Korea"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Lee", 
            "givenName": "Sang-Hyun", 
            "id": "sg:person.01137262612.44", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01137262612.44"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "LG Corporation (South Korea)", 
              "id": "https://www.grid.ac/institutes/grid.464630.3", 
              "name": [
                "Corporate R&D, LG Chem, Ltd./Research Park, 104-1 Moonji-dong, Yuseong-gu, 305-380, Daejeon, Republic of Korea"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Park", 
            "givenName": "Si Jae", 
            "id": "sg:person.01170712106.39", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01170712106.39"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Korea Advanced Institute of Science and Technology", 
              "id": "https://www.grid.ac/institutes/grid.37172.30", 
              "name": [
                "Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Program), BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, 305-701, Daejeon, Republic of Korea", 
                "Department of Bio and Brain Engineering, and Bioinformatics Research Center, Korea Advanced Institute of Science and Technology, 305-701, Daejeon, Republic of Korea"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Lee", 
            "givenName": "Sang Yup", 
            "id": "sg:person.0664754031.03", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0664754031.03"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of Ulsan", 
              "id": "https://www.grid.ac/institutes/grid.267370.7", 
              "name": [
                "School of Chemical Engineering & Bioengineering, University of Ulsan, 29 Mugeo-dong, Nam-gu, 680-749, Ulsan, Republic of Korea"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Hong", 
            "givenName": "Soon Ho", 
            "id": "sg:person.01070167661.37", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01070167661.37"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1246/cl.1987.2187", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001574079"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/bit.10733", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004541288"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0163-7827(98)00022-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006834559"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1574-6968.2002.tb11286.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010853257"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1574-6968.2002.tb11286.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010853257"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/bit.1115", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011693313"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/mabi.200300096", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011937743"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/aem.70.7.3807-3813.2004", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016414499"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/(sici)1097-0290(19991105)65:3<363::aid-bit15>3.0.co;2-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019766985"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1574-6968.1988.tb02607.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020388244"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1574-6968.1988.tb02607.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020388244"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1271/bbb1961.53.2009", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022366955"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0040-4020(01)96003-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022767186"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/bit.10139", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029220632"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/bit.1098", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031178081"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/jb.185.18.5391-5397.2003", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034903751"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/aem.69.6.3421-3426.2003", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035631638"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00253-007-1063-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036697342", 
              "https://doi.org/10.1007/s00253-007-1063-0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00253-007-1063-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036697342", 
              "https://doi.org/10.1007/s00253-007-1063-0"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1574-6968.2003.tb11498.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041313477"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1574-6968.2003.tb11498.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041313477"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0040-4020(01)92449-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043196129"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0141-0229(00)00146-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053333290"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1097(95)00125-o", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1054561773"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/ja00253a051", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055717847"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/ja00356a041", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1055726072"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1083355113", 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2008-06", 
        "datePublishedReg": "2008-06-01", 
        "description": "A biosynthetic pathway for the production of (S)-3-hydroxybutyric acid (S3HB) from glucose was established in recombinant Escherichia coli by introducing the beta-ketothiolase gene from Ralstonia eutropha H16, the (S)-3-hydroxybutyryl-CoA dehydrogenase gene from R. eutropha H16, or Clostridium acetobutylicum ATCC824, and the 3-hydroxyisobutyryl-CoA hydrolase gene from Bacillus cereus ATCC14579. Artificial operon consisting of these genes was constructed and was expressed in E. coli BL21 (DE3) codon plus under T7 promoter by isopropyl beta-D: -thiogalactoside (IPTG) induction. Recombinant E. coli BL21 (DE3) codon plus expressing the beta-ketothiolase gene, the (S)-3-hydroxybutyryl-CoA dehydrogenase gene, and the 3-hydroxyisobutyryl-CoA hydrolase gene could synthesize enantiomerically pure S3HB to the concentration of 0.61 g l(-1) from 20 g l(-1) of glucose in Luria-Bertani medium. Fed-batch cultures of recombinant E. coli BL21 (DE3) codon plus were carried out to achieve higher titer of S3HB with varying induction time and glucose concentration during fermentation. Protein expression was induced by addition of 1 mM IPTG when cell concentration reached 10 and 20 g l(-1) (OD(600) = 30 and 60), respectively. When protein expression was induced at 60 of OD(600) and glucose was fed to the concentration of 15 g l(-1), 10.3 g l(-1) of S3HB was obtained in 38 h with the S3HB productivity of 0.21 g l(-1)h(-1). Lowering glucose concentration to 5 g l(-1) and induction of protein expression at 30 of OD(600) significantly reduced final S3HB concentration to 3.7 g l(-1), which also resulted in the decrease of the S3HB productivity to 0.05 g l(-1)h(-1).", 
        "genre": "research_article", 
        "id": "sg:pub.10.1007/s00253-008-1473-7", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1083533", 
            "issn": [
              "0175-7598", 
              "1432-0614"
            ], 
            "name": "Applied Microbiology and Biotechnology", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "4", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "79"
          }
        ], 
        "name": "Biosynthesis of enantiopure (S)-3-hydroxybutyric acid in metabolically engineered Escherichia coli", 
        "pagination": "633-641", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "7c66535aa21455b056373228075f841419130b4669a360072cec2690d5b173d1"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "18461320"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "8406612"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/s00253-008-1473-7"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1026112726"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/s00253-008-1473-7", 
          "https://app.dimensions.ai/details/publication/pub.1026112726"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T14:31", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000373_0000000373/records_13096_00000001.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://link.springer.com/10.1007%2Fs00253-008-1473-7"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00253-008-1473-7'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00253-008-1473-7'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00253-008-1473-7'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00253-008-1473-7'


     

    This table displays all metadata directly associated to this object as RDF triples.

    226 TRIPLES      21 PREDICATES      67 URIs      36 LITERALS      24 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/s00253-008-1473-7 schema:about N0b4d0ca5c50e4a8284498ce67ebc042a
    2 N2368073763484e4cab155f48d92b5562
    3 N2712bc800ab146f6b12c39c5bcc8c713
    4 N2dc0a3b2c8094190b27215c11db19cf3
    5 N437e384f14b8481592911c4d1a5b02bb
    6 N576701fd7933478fada2afa7bfdea0c9
    7 N57b988774f3942c69d183d6fce6ef01f
    8 N816ac67fe67f46209b4eb8f719ea4c77
    9 N81d744cdb3614aa48e2ed773430343a2
    10 N9fc09578bc034a9084995644238d06cf
    11 Na506d78ff8c44338bece6d0d613a006b
    12 Nb435cd330add4e068ae92aa30b4104a4
    13 Nb685b509652343158b08e77c03d95316
    14 Nd374a1782dff414f97c81b1f0e04b125
    15 Neff4dd56b12a4099a5664a12ebfee9b0
    16 anzsrc-for:06
    17 anzsrc-for:0604
    18 schema:author Na09d9e92a7f545928d5891fc5d36fd8b
    19 schema:citation sg:pub.10.1007/s00253-007-1063-0
    20 https://app.dimensions.ai/details/publication/pub.1083355113
    21 https://doi.org/10.1002/(sici)1097-0290(19991105)65:3<363::aid-bit15>3.0.co;2-1
    22 https://doi.org/10.1002/bit.10139
    23 https://doi.org/10.1002/bit.10733
    24 https://doi.org/10.1002/bit.1098
    25 https://doi.org/10.1002/bit.1115
    26 https://doi.org/10.1002/mabi.200300096
    27 https://doi.org/10.1016/0378-1097(95)00125-o
    28 https://doi.org/10.1016/s0040-4020(01)92449-4
    29 https://doi.org/10.1016/s0040-4020(01)96003-x
    30 https://doi.org/10.1016/s0141-0229(00)00146-0
    31 https://doi.org/10.1016/s0163-7827(98)00022-8
    32 https://doi.org/10.1021/ja00253a051
    33 https://doi.org/10.1021/ja00356a041
    34 https://doi.org/10.1111/j.1574-6968.1988.tb02607.x
    35 https://doi.org/10.1111/j.1574-6968.2002.tb11286.x
    36 https://doi.org/10.1111/j.1574-6968.2003.tb11498.x
    37 https://doi.org/10.1128/aem.69.6.3421-3426.2003
    38 https://doi.org/10.1128/aem.70.7.3807-3813.2004
    39 https://doi.org/10.1128/jb.185.18.5391-5397.2003
    40 https://doi.org/10.1246/cl.1987.2187
    41 https://doi.org/10.1271/bbb1961.53.2009
    42 schema:datePublished 2008-06
    43 schema:datePublishedReg 2008-06-01
    44 schema:description A biosynthetic pathway for the production of (S)-3-hydroxybutyric acid (S3HB) from glucose was established in recombinant Escherichia coli by introducing the beta-ketothiolase gene from Ralstonia eutropha H16, the (S)-3-hydroxybutyryl-CoA dehydrogenase gene from R. eutropha H16, or Clostridium acetobutylicum ATCC824, and the 3-hydroxyisobutyryl-CoA hydrolase gene from Bacillus cereus ATCC14579. Artificial operon consisting of these genes was constructed and was expressed in E. coli BL21 (DE3) codon plus under T7 promoter by isopropyl beta-D: -thiogalactoside (IPTG) induction. Recombinant E. coli BL21 (DE3) codon plus expressing the beta-ketothiolase gene, the (S)-3-hydroxybutyryl-CoA dehydrogenase gene, and the 3-hydroxyisobutyryl-CoA hydrolase gene could synthesize enantiomerically pure S3HB to the concentration of 0.61 g l(-1) from 20 g l(-1) of glucose in Luria-Bertani medium. Fed-batch cultures of recombinant E. coli BL21 (DE3) codon plus were carried out to achieve higher titer of S3HB with varying induction time and glucose concentration during fermentation. Protein expression was induced by addition of 1 mM IPTG when cell concentration reached 10 and 20 g l(-1) (OD(600) = 30 and 60), respectively. When protein expression was induced at 60 of OD(600) and glucose was fed to the concentration of 15 g l(-1), 10.3 g l(-1) of S3HB was obtained in 38 h with the S3HB productivity of 0.21 g l(-1)h(-1). Lowering glucose concentration to 5 g l(-1) and induction of protein expression at 30 of OD(600) significantly reduced final S3HB concentration to 3.7 g l(-1), which also resulted in the decrease of the S3HB productivity to 0.05 g l(-1)h(-1).
    45 schema:genre research_article
    46 schema:inLanguage en
    47 schema:isAccessibleForFree false
    48 schema:isPartOf N9a19b29cc14143878cfe1c47d4e63292
    49 Nfda4da94a9654bdba1555f18325b6630
    50 sg:journal.1083533
    51 schema:name Biosynthesis of enantiopure (S)-3-hydroxybutyric acid in metabolically engineered Escherichia coli
    52 schema:pagination 633-641
    53 schema:productId N14e711d36dfd42f08bac79cdc41e87e6
    54 N35b3d0ac005a4c86a094357a0cbc11c5
    55 N83bd524c97bb41c180ad1a22716c1f44
    56 N9ae571485f70444991d322c108f7b555
    57 Ne3d89d1c664a43bdabba885a9dcb4523
    58 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026112726
    59 https://doi.org/10.1007/s00253-008-1473-7
    60 schema:sdDatePublished 2019-04-11T14:31
    61 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    62 schema:sdPublisher N9e743fe7b8204e669912807185087bb1
    63 schema:url http://link.springer.com/10.1007%2Fs00253-008-1473-7
    64 sgo:license sg:explorer/license/
    65 sgo:sdDataset articles
    66 rdf:type schema:ScholarlyArticle
    67 N01581a38d8cd4505a48cea769fd8e854 rdf:first sg:person.01070167661.37
    68 rdf:rest rdf:nil
    69 N0b4d0ca5c50e4a8284498ce67ebc042a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    70 schema:name 3-Hydroxybutyric Acid
    71 rdf:type schema:DefinedTerm
    72 N14e711d36dfd42f08bac79cdc41e87e6 schema:name pubmed_id
    73 schema:value 18461320
    74 rdf:type schema:PropertyValue
    75 N18e4eeef4fd44730863c2de63dac78e5 rdf:first sg:person.0664754031.03
    76 rdf:rest N01581a38d8cd4505a48cea769fd8e854
    77 N2368073763484e4cab155f48d92b5562 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    78 schema:name Genetic Engineering
    79 rdf:type schema:DefinedTerm
    80 N2712bc800ab146f6b12c39c5bcc8c713 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    81 schema:name Glucose
    82 rdf:type schema:DefinedTerm
    83 N2dc0a3b2c8094190b27215c11db19cf3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    84 schema:name Biosynthetic Pathways
    85 rdf:type schema:DefinedTerm
    86 N35b3d0ac005a4c86a094357a0cbc11c5 schema:name dimensions_id
    87 schema:value pub.1026112726
    88 rdf:type schema:PropertyValue
    89 N437e384f14b8481592911c4d1a5b02bb schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    90 schema:name Bacterial Proteins
    91 rdf:type schema:DefinedTerm
    92 N576701fd7933478fada2afa7bfdea0c9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    93 schema:name 3-Hydroxyacyl CoA Dehydrogenases
    94 rdf:type schema:DefinedTerm
    95 N57b988774f3942c69d183d6fce6ef01f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    96 schema:name Cupriavidus necator
    97 rdf:type schema:DefinedTerm
    98 N816ac67fe67f46209b4eb8f719ea4c77 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    99 schema:name Acetyl-CoA C-Acyltransferase
    100 rdf:type schema:DefinedTerm
    101 N81d744cdb3614aa48e2ed773430343a2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    102 schema:name Escherichia coli
    103 rdf:type schema:DefinedTerm
    104 N83bd524c97bb41c180ad1a22716c1f44 schema:name nlm_unique_id
    105 schema:value 8406612
    106 rdf:type schema:PropertyValue
    107 N9a19b29cc14143878cfe1c47d4e63292 schema:issueNumber 4
    108 rdf:type schema:PublicationIssue
    109 N9ae571485f70444991d322c108f7b555 schema:name doi
    110 schema:value 10.1007/s00253-008-1473-7
    111 rdf:type schema:PropertyValue
    112 N9e743fe7b8204e669912807185087bb1 schema:name Springer Nature - SN SciGraph project
    113 rdf:type schema:Organization
    114 N9fc09578bc034a9084995644238d06cf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    115 schema:name Bioreactors
    116 rdf:type schema:DefinedTerm
    117 Na09d9e92a7f545928d5891fc5d36fd8b rdf:first sg:person.01137262612.44
    118 rdf:rest Nf4aa082662d44c28850349d8f947e1cf
    119 Na506d78ff8c44338bece6d0d613a006b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    120 schema:name Clostridium acetobutylicum
    121 rdf:type schema:DefinedTerm
    122 Nb435cd330add4e068ae92aa30b4104a4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    123 schema:name Industrial Microbiology
    124 rdf:type schema:DefinedTerm
    125 Nb685b509652343158b08e77c03d95316 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    126 schema:name Bacillus cereus
    127 rdf:type schema:DefinedTerm
    128 Nd374a1782dff414f97c81b1f0e04b125 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    129 schema:name Biomass
    130 rdf:type schema:DefinedTerm
    131 Ne3d89d1c664a43bdabba885a9dcb4523 schema:name readcube_id
    132 schema:value 7c66535aa21455b056373228075f841419130b4669a360072cec2690d5b173d1
    133 rdf:type schema:PropertyValue
    134 Neff4dd56b12a4099a5664a12ebfee9b0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    135 schema:name Thiolester Hydrolases
    136 rdf:type schema:DefinedTerm
    137 Nf4aa082662d44c28850349d8f947e1cf rdf:first sg:person.01170712106.39
    138 rdf:rest N18e4eeef4fd44730863c2de63dac78e5
    139 Nfda4da94a9654bdba1555f18325b6630 schema:volumeNumber 79
    140 rdf:type schema:PublicationVolume
    141 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    142 schema:name Biological Sciences
    143 rdf:type schema:DefinedTerm
    144 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    145 schema:name Genetics
    146 rdf:type schema:DefinedTerm
    147 sg:journal.1083533 schema:issn 0175-7598
    148 1432-0614
    149 schema:name Applied Microbiology and Biotechnology
    150 rdf:type schema:Periodical
    151 sg:person.01070167661.37 schema:affiliation https://www.grid.ac/institutes/grid.267370.7
    152 schema:familyName Hong
    153 schema:givenName Soon Ho
    154 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01070167661.37
    155 rdf:type schema:Person
    156 sg:person.01137262612.44 schema:affiliation https://www.grid.ac/institutes/grid.464630.3
    157 schema:familyName Lee
    158 schema:givenName Sang-Hyun
    159 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01137262612.44
    160 rdf:type schema:Person
    161 sg:person.01170712106.39 schema:affiliation https://www.grid.ac/institutes/grid.464630.3
    162 schema:familyName Park
    163 schema:givenName Si Jae
    164 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01170712106.39
    165 rdf:type schema:Person
    166 sg:person.0664754031.03 schema:affiliation https://www.grid.ac/institutes/grid.37172.30
    167 schema:familyName Lee
    168 schema:givenName Sang Yup
    169 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0664754031.03
    170 rdf:type schema:Person
    171 sg:pub.10.1007/s00253-007-1063-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036697342
    172 https://doi.org/10.1007/s00253-007-1063-0
    173 rdf:type schema:CreativeWork
    174 https://app.dimensions.ai/details/publication/pub.1083355113 schema:CreativeWork
    175 https://doi.org/10.1002/(sici)1097-0290(19991105)65:3<363::aid-bit15>3.0.co;2-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019766985
    176 rdf:type schema:CreativeWork
    177 https://doi.org/10.1002/bit.10139 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029220632
    178 rdf:type schema:CreativeWork
    179 https://doi.org/10.1002/bit.10733 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004541288
    180 rdf:type schema:CreativeWork
    181 https://doi.org/10.1002/bit.1098 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031178081
    182 rdf:type schema:CreativeWork
    183 https://doi.org/10.1002/bit.1115 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011693313
    184 rdf:type schema:CreativeWork
    185 https://doi.org/10.1002/mabi.200300096 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011937743
    186 rdf:type schema:CreativeWork
    187 https://doi.org/10.1016/0378-1097(95)00125-o schema:sameAs https://app.dimensions.ai/details/publication/pub.1054561773
    188 rdf:type schema:CreativeWork
    189 https://doi.org/10.1016/s0040-4020(01)92449-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043196129
    190 rdf:type schema:CreativeWork
    191 https://doi.org/10.1016/s0040-4020(01)96003-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1022767186
    192 rdf:type schema:CreativeWork
    193 https://doi.org/10.1016/s0141-0229(00)00146-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053333290
    194 rdf:type schema:CreativeWork
    195 https://doi.org/10.1016/s0163-7827(98)00022-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006834559
    196 rdf:type schema:CreativeWork
    197 https://doi.org/10.1021/ja00253a051 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055717847
    198 rdf:type schema:CreativeWork
    199 https://doi.org/10.1021/ja00356a041 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055726072
    200 rdf:type schema:CreativeWork
    201 https://doi.org/10.1111/j.1574-6968.1988.tb02607.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1020388244
    202 rdf:type schema:CreativeWork
    203 https://doi.org/10.1111/j.1574-6968.2002.tb11286.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1010853257
    204 rdf:type schema:CreativeWork
    205 https://doi.org/10.1111/j.1574-6968.2003.tb11498.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1041313477
    206 rdf:type schema:CreativeWork
    207 https://doi.org/10.1128/aem.69.6.3421-3426.2003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035631638
    208 rdf:type schema:CreativeWork
    209 https://doi.org/10.1128/aem.70.7.3807-3813.2004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016414499
    210 rdf:type schema:CreativeWork
    211 https://doi.org/10.1128/jb.185.18.5391-5397.2003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034903751
    212 rdf:type schema:CreativeWork
    213 https://doi.org/10.1246/cl.1987.2187 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001574079
    214 rdf:type schema:CreativeWork
    215 https://doi.org/10.1271/bbb1961.53.2009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022366955
    216 rdf:type schema:CreativeWork
    217 https://www.grid.ac/institutes/grid.267370.7 schema:alternateName University of Ulsan
    218 schema:name School of Chemical Engineering & Bioengineering, University of Ulsan, 29 Mugeo-dong, Nam-gu, 680-749, Ulsan, Republic of Korea
    219 rdf:type schema:Organization
    220 https://www.grid.ac/institutes/grid.37172.30 schema:alternateName Korea Advanced Institute of Science and Technology
    221 schema:name Department of Bio and Brain Engineering, and Bioinformatics Research Center, Korea Advanced Institute of Science and Technology, 305-701, Daejeon, Republic of Korea
    222 Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 Program), BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, Institute for the BioCentury, 305-701, Daejeon, Republic of Korea
    223 rdf:type schema:Organization
    224 https://www.grid.ac/institutes/grid.464630.3 schema:alternateName LG Corporation (South Korea)
    225 schema:name Corporate R&D, LG Chem, Ltd./Research Park, 104-1 Moonji-dong, Yuseong-gu, 305-380, Daejeon, Republic of Korea
    226 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...