Corynebacterium glutamicum tailored for high-yield L-valine production View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2008-06

AUTHORS

Bastian Blombach, Mark E. Schreiner, Tobias Bartek, Marco Oldiges, Bernhard J. Eikmanns

ABSTRACT

We recently engineered the wild type of Corynebacterium glutamicum for the growth-decoupled production of L: -valine from glucose by inactivation of the pyruvate dehydrogenase complex and additional overexpression of the ilvBNCE genes, encoding the L-valine biosynthetic enzymes acetohydroxyacid synthase, isomeroreductase, and transaminase B. Based on the first generation of pyruvate-dehydrogenase-complex-deficient C. glutamicum strains, a second generation of high-yield L-valine producers was constructed by successive deletion of the genes encoding pyruvate:quinone oxidoreductase, phosphoglucose isomerase, and pyruvate carboxylase and overexpression of ilvBNCE. In fed-batch fermentations at high cell densities, the newly constructed strains produced up to 410 mM (48 g/l) L-valine, showed a maximum yield of 0.75 to 0.86 mol/mol (0.49 to 0.56 g/g) of glucose in the production phase and, in contrast to the first generation strains, excreted neither pyruvate nor any other by-product tested. More... »

PAGES

471-479

Journal

TITLE

Applied Microbiology and Biotechnology

ISSUE

3

VOLUME

79

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00253-008-1444-z

DOI

http://dx.doi.org/10.1007/s00253-008-1444-z

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1015564480

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/18379776


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