Production of isopropanol by metabolically engineered Escherichia coli View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2008-01

AUTHORS

Toru Jojima, Masayuki Inui, Hideaki Yukawa

ABSTRACT

A genetically engineered strain of Escherichia coli JM109 harboring the isopropanol-producing pathway consisting of five genes encoding four enzymes, thiolase, coenzyme A (CoA) transferase, acetoacetate decarboxylase from Clostridium acetobutylicum ATCC 824, and primary-secondary alcohol dehydrogenase from C. beijerinckii NRRL B593, produced up to 227 mM of isopropanol from glucose under aerobic fed-batch culture conditions. Acetate production by the engineered strain was approximately one sixth that produced by a control E. coli strain bearing an expression vector without the clostridial genes. These results demonstrate a functional isopropanol-producing pathway in E. coli and consequently carbon flux from acetyl-CoA directed to isopropanol instead of acetate. This is the first report on isopropanol production by genetically engineered microorganism under aerobic culture conditions. More... »

PAGES

1219-1224

Journal

TITLE

Applied Microbiology and Biotechnology

ISSUE

6

VOLUME

77

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00253-007-1246-8

    DOI

    http://dx.doi.org/10.1007/s00253-007-1246-8

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1051740809

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/17987288


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