Microbial production of R-3-hydroxybutyric acid by recombinant E. coli harboring genes of phbA, phbB, and tesB View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2007-09

AUTHORS

Qian Liu, Shao-Ping Ouyang, Ahleum Chung, Qiong Wu, Guo-Qiang Chen

ABSTRACT

Production of R-3-hydroxybutyric acid (3HB) was observed when genes of beta-ketothiolase (PhbA), acetoacetyl CoA reductase (PhbB), and thioesterase II (TesB) were jointly expressed in Escherichia coli. TesB, generally regarded as a medium chain length acyl CoA thioesterase, was found, for the first time, to play an important role for transforming short chain length 3-hydroxybutyrate-CoA to its free fatty acid, namely, 3HB. E. coli BW25113 (pSPB01) harboring phbA, phbB, and tesB genes produced approximately 4 g/l 3HB in shake flask culture within 24 h with glucose used as a carbon source. Under anaerobic growth conditions, 3HB production was found to be more effective, achieving 0.47 g 3HB/g glucose compared with only 0.32 g 3HB/g glucose obtained from aerobic process. When growth was conducted on sodium gluconate, 6 g/l 3HB was obtained. In a 24-h fed-batch growth process conducted in a 6-l fermentor containing 3 l glucose mineral medium, 12 g/l 3HB was produced from 17 g/l cell dry weight (CDW). This was the highest 3HB productivity achieved by a one-stage fermentation process for 3HB production. More... »

PAGES

811-818

Journal

TITLE

Applied Microbiology and Biotechnology

ISSUE

4

VOLUME

76

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00253-007-1063-0

    DOI

    http://dx.doi.org/10.1007/s00253-007-1063-0

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1036697342

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/17609944


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    HOW TO GET THIS DATA PROGRAMMATICALLY:

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    RDF/XML is a standard XML format for linked data.

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