Isolation and properties of a levo-lactonase from Fusarium proliferatum ECU2002: a robust biocatalyst for production of chiral lactones View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2007-07

AUTHORS

Xian Zhang, Jian-He Xu, Yi Xu, Jiang Pan

ABSTRACT

A fungus strain ECU2002, capable of enantioselectively hydrolyzing chiral lactones to optically pure hydroxy acids, was newly isolated from soil samples through two steps of screening and identified as Fusarium proliferatum (Matsushima) Nirenberg. From the crude extract of F. proliferatum ECU2002, a novel levo-lactonase was purified to homogeneity, with a purification factor of 460-folds and an overall yield of 9.7%, by ultrafiltration, acetone precipitation, and chromatographic separation through DEAE-Toyopearl, Butyl-Toyopearl, Hydroxyapatite, Toyoscreen-Super Q, and TSK-gel columns. The purified enzyme is a monomer; with a molecular mass of ca 68 kDa and a pI of 5.7 as determined by two-dimensional electrophoresis. The catalytic performance of the partially purified levo-lactonase was investigated, giving temperature and pH optima at 50 degrees C and 7.5, respectively, for gamma-butyrolactone hydrolysis. The substrate specificity of the partially purified lactonase was also examined using several useful lactones, among which alpha-hydroxy-gamma-butyrolactone was the best substrate, with 448-fold higher lactonase activity as compared to gamma-butyrolactone. The F. proliferatum lactonase preferentially hydrolyzed the levo enantiomer of butyrolactones, including beta-butyrolactone, alpha-hydroxy-gamma-butyrolactone, alpha-hydroxy-beta,beta-dimethyl-gamma-butyrolactone (pantolactone), and beta-hydroxy-gamma-butyrolactone, affording (+)-hydroxy acids in high (94.8 approximately 98.2%) enantiomeric excesses (ee) and good conversions (38.2 approximately 44.2%). A simple immobilization of the crude lactonase with glutaraldehyde cross-linking led to a stable and easy-to-handle biocatalyst for catalytic resolution of chiral lactones. The immobilized lactonase also performed quite well in repeated batch resolution of dl-pantolactone at a concentration of 35% (w/v), retaining 67% of initial activity after ten cycles of reaction (corresponding to a half life of 20 cycles) and affording the product in 94 approximately 97% ee, which can be easily enhanced to >99% ee after the d-hydroxy acid was chemically converted into l-lactone and crystallized. More... »

PAGES

1087-1094

Journal

TITLE

Applied Microbiology and Biotechnology

ISSUE

5

VOLUME

75

Related Patents

  • Microorganisms For The Production Of 1,4-Butanediol
  • Microorganisms And Methods For The Co-Production Of Isopropanol And 1,4-Butanediol
  • Microorganisms And Methods For The Coproduction 1,4-Butanediol And Gamma-Butyrolactone
  • Microorganisms For The Production Of Methacrylic Acid
  • Microorganisms And Methods For Production Of 4-Hydroxybutyrate, 1,4-Butanediol And Related Compounds
  • Microorganisms And Methods For Conversion Of Syngas And Other Carbon Sources To Useful Products
  • Semi-Synthetic Terephthalic Acid Via Microorganisms That Produce Muconic Acid
  • Compositions And Methods For The Biosynthesis Of 1,4-Butanediol And Its Precursors
  • Microorganisms For The Production Of Adipic Acid And Other Compounds
  • Methods And Organisms For Utilizing Synthesis Gas Or Other Gaseous Carbon Sources And Methanol
  • Microorganisms And Methods For The Biosynthesis Of Butadiene
  • Microorganisms For The Production Of 1,4-Butanediol
  • Microorganisms And Methods For The Biosynthesis Of Propylene
  • Semi-Synthetic Terephthalic Acid Via Microorganisms That Produce Muconic Acid
  • Microorganisms And Methods For The Biosynthesis Of Adipate, Hexamethylenediamine And 6-Aminocaproic Acid
  • Process Of Separating Components Of A Fermentation Broth
  • Microorganisms And Methods For The Production Of Caprolactone
  • Compositions And Methods For The Biosynthesis Of 1,4-Butanediol And Its Precursors
  • Methods For The Synthesis Of Olefins And Derivatives
  • Microorganisms And Methods For Carbon-Efficient Biosynthesis Of Mek And 2-Butanol
  • Microorganisms For The Production Of 1,4-Butanediol And Related Methods
  • Compositions And Methods For The Biosynthesis Of 1,4-Butanediol And Its Precursors
  • Microorganisms For The Production Of 2-Hydroxyisobutyric Acid
  • Microorganisms For The Production Of Methacrylic Acid
  • Microorganisms And Methods For The Coproduction 1,4-Butanediol And Gamma-Butyrolactone
  • Microorganisms And Methods For The Biosynthesis Of Fumarate, Malate, And Acrylate
  • Methods And Organisms For Utilizing Synthesis Gas Or Other Gaseous Carbon Sources And Methanol
  • Microorganisms And Methods For The Coproduction 1,4-Butanediol And Gamma-Butyrolactone
  • Microorganisms And Methods For The Biosynthesis Of Aromatics, 2,4-Pentadienoate And 1,3-Butadiene
  • Microorganisms And Methods For The Production Of Caprolactone
  • Organisms For The Production Of 1,3-Butanediol
  • Microbial Organisms Comprising Exogenous Nucleic Acids Encoding Reductive Tca Pathway Enzymes
  • Microorganisms For The Production Of Methacrylic Acid
  • Microorganisms For The Production Of 1,4-Butanediol, 4-Hydroxybutanal, 4-Hydroxybutyryl-Coa, Putrescine And Related Compounds, And Methods Related Thereto
  • Methods And Organisms For The Growth-Coupled Production Of 1,4-Butanediol
  • Microorganisms For The Production Of 1,4-Butanediol
  • Compositions And Methods For The Biosynthesis Of 1,4-Butanediol And Its Precursors
  • Microorganisms And Methods For The Production Of Caprolactone
  • Microorganisms For The Production Of Adipic Acid And Other Compounds
  • Methods And Organisms For Utilizing Synthesis Gas Or Other Gaseous Carbon Sources And Methanol
  • Methods For Increasing Product Yields
  • Compositions And Methods For The Biosynthesis Of 1,4-Butanediol And Its Precursors
  • Microorganisms For The Production Of 1,4-Butanediol, 4-Hydroxybutanal, 4-Hydroxybutyryl-Coa, Putrescine And Related Compounds, And Methods Related Thereto
  • Methods And Organisms For Utilizing Synthesis Gas Or Other Gaseous Carbon Sources And Methanol
  • Microorganisms For The Production Of Adipic Acid And Other Compounds
  • Primary Alcohol Producing Organisms
  • Primary Alcohol Producing Organisms
  • Methods And Organisms For The Growth-Coupled Production Of 1,4-Butanediol
  • Organisms For The Production Of Isopropanol, N-Butanol, And Isobutanol
  • Microorganisms For The Production Of 1,4-Butanediol
  • Microorganisms For The Production Of 1,4-Butanediol And Related Methods
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00253-007-0941-9

    DOI

    http://dx.doi.org/10.1007/s00253-007-0941-9

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1002209715

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/17530243


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0306", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Physical Chemistry (incl. Structural)", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/03", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Chemical Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Amino Acid Sequence", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Enzymes", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Fusarium", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Lactones", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Molecular Conformation", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Molecular Sequence Data", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "East China University of Science and Technology", 
              "id": "https://www.grid.ac/institutes/grid.28056.39", 
              "name": [
                "Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 200237, Shanghai, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Zhang", 
            "givenName": "Xian", 
            "id": "sg:person.015623536651.64", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015623536651.64"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "East China University of Science and Technology", 
              "id": "https://www.grid.ac/institutes/grid.28056.39", 
              "name": [
                "Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 200237, Shanghai, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Xu", 
            "givenName": "Jian-He", 
            "id": "sg:person.01320335040.67", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01320335040.67"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "East China University of Science and Technology", 
              "id": "https://www.grid.ac/institutes/grid.28056.39", 
              "name": [
                "Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 200237, Shanghai, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Xu", 
            "givenName": "Yi", 
            "id": "sg:person.01365036241.45", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01365036241.45"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "East China University of Science and Technology", 
              "id": "https://www.grid.ac/institutes/grid.28056.39", 
              "name": [
                "Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 200237, Shanghai, China"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Pan", 
            "givenName": "Jiang", 
            "id": "sg:person.014401137627.32", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014401137627.32"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1016/s0378-4347(00)86033-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001783778"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0168-1656(01)00359-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002659511"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1271/bbb.64.1255", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006929833"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/1615-4169(200212)344:10<1103::aid-adsc1103>3.0.co;2-r", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011355342"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1046/j.1432-1327.2000.00889.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013683145"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1271/bbb1961.51.3011", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1014838782"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.jbiotec.2005.03.015", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1017056527"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0957-4166(98)00281-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018031031"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0141-0229(98)00086-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020862519"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1271/bbb1961.51.289", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1028804364"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0141-0229(87)90136-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029910220"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0141-0229(87)90136-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1029910220"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s002030100283", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031960956", 
              "https://doi.org/10.1007/s002030100283"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1070/mc2003v013n03abeh001755", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033856978"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1070/mc2003v013n03abeh001755", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033856978"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1432-1033.1992.tb17300.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036433832"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0141-0229(93)90053-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037797630"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0141-0229(93)90053-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037797630"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0040-4039(00)98627-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038115722"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0168-1656(97)00107-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039031794"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00203-003-0597-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043429092", 
              "https://doi.org/10.1007/s00203-003-0597-5"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00169925", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044188776", 
              "https://doi.org/10.1007/bf00169925"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00169925", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044188776", 
              "https://doi.org/10.1007/bf00169925"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.procbio.2004.06.060", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048850857"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/j.enzmictec.2006.05.004", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049039207"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/(sici)1099-0690(199911)1999:11<2705::aid-ejoc2705>3.0.co;2-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049398507"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/(sici)1099-0690(199911)1999:11<2705::aid-ejoc2705>3.0.co;2-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049398507"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0032-9592(02)00164-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050220650"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0032-9592(02)00164-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050220650"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0141-0229(88)90062-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052458146"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0141-0229(88)90062-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052458146"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0957-4166(94)80100-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053732472"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/ol026489d", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1056242983"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1021/ol026489d", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1056242983"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1055/s-1988-27577", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1057392192"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1074890306", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1079943154", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1080459604", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1083382082", 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2007-07", 
        "datePublishedReg": "2007-07-01", 
        "description": "A fungus strain ECU2002, capable of enantioselectively hydrolyzing chiral lactones to optically pure hydroxy acids, was newly isolated from soil samples through two steps of screening and identified as Fusarium proliferatum (Matsushima) Nirenberg. From the crude extract of F. proliferatum ECU2002, a novel levo-lactonase was purified to homogeneity, with a purification factor of 460-folds and an overall yield of 9.7%, by ultrafiltration, acetone precipitation, and chromatographic separation through DEAE-Toyopearl, Butyl-Toyopearl, Hydroxyapatite, Toyoscreen-Super Q, and TSK-gel columns. The purified enzyme is a monomer; with a molecular mass of ca 68 kDa and a pI of 5.7 as determined by two-dimensional electrophoresis. The catalytic performance of the partially purified levo-lactonase was investigated, giving temperature and pH optima at 50 degrees C and 7.5, respectively, for gamma-butyrolactone hydrolysis. The substrate specificity of the partially purified lactonase was also examined using several useful lactones, among which alpha-hydroxy-gamma-butyrolactone was the best substrate, with 448-fold higher lactonase activity as compared to gamma-butyrolactone. The F. proliferatum lactonase preferentially hydrolyzed the levo enantiomer of butyrolactones, including beta-butyrolactone, alpha-hydroxy-gamma-butyrolactone, alpha-hydroxy-beta,beta-dimethyl-gamma-butyrolactone (pantolactone), and beta-hydroxy-gamma-butyrolactone, affording (+)-hydroxy acids in high (94.8 approximately 98.2%) enantiomeric excesses (ee) and good conversions (38.2 approximately 44.2%). A simple immobilization of the crude lactonase with glutaraldehyde cross-linking led to a stable and easy-to-handle biocatalyst for catalytic resolution of chiral lactones. The immobilized lactonase also performed quite well in repeated batch resolution of dl-pantolactone at a concentration of 35% (w/v), retaining 67% of initial activity after ten cycles of reaction (corresponding to a half life of 20 cycles) and affording the product in 94 approximately 97% ee, which can be easily enhanced to >99% ee after the d-hydroxy acid was chemically converted into l-lactone and crystallized.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1007/s00253-007-0941-9", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1083533", 
            "issn": [
              "0175-7598", 
              "1432-0614"
            ], 
            "name": "Applied Microbiology and Biotechnology", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "5", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "75"
          }
        ], 
        "name": "Isolation and properties of a levo-lactonase from Fusarium proliferatum ECU2002: a robust biocatalyst for production of chiral lactones", 
        "pagination": "1087-1094", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "0a85713429b5fbd053cc2e8ca41e8e0788936f06f57954a7fa96662f3be0e058"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "17530243"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "8406612"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/s00253-007-0941-9"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1002209715"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/s00253-007-0941-9", 
          "https://app.dimensions.ai/details/publication/pub.1002209715"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-10T21:36", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8687_00000509.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://link.springer.com/10.1007%2Fs00253-007-0941-9"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00253-007-0941-9'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00253-007-0941-9'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00253-007-0941-9'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00253-007-0941-9'


     

    This table displays all metadata directly associated to this object as RDF triples.

    206 TRIPLES      21 PREDICATES      66 URIs      27 LITERALS      15 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/s00253-007-0941-9 schema:about N004627c68c104ba8bf3e739d37f17f26
    2 N8e71d5312b30470e9d715fb72725da12
    3 Na96740cbf2814c9e9d25701e35890c56
    4 Nb32d73720471422cbb4583686a876500
    5 Nba71c74e463d4210baf6c3188b0acca2
    6 Nd612490da0fe48519fbd659364ce9bc8
    7 anzsrc-for:03
    8 anzsrc-for:0306
    9 schema:author N60fd1ab02b08405e88db81b8a11578f5
    10 schema:citation sg:pub.10.1007/bf00169925
    11 sg:pub.10.1007/s00203-003-0597-5
    12 sg:pub.10.1007/s002030100283
    13 https://app.dimensions.ai/details/publication/pub.1074890306
    14 https://app.dimensions.ai/details/publication/pub.1079943154
    15 https://app.dimensions.ai/details/publication/pub.1080459604
    16 https://app.dimensions.ai/details/publication/pub.1083382082
    17 https://doi.org/10.1002/(sici)1099-0690(199911)1999:11<2705::aid-ejoc2705>3.0.co;2-4
    18 https://doi.org/10.1002/1615-4169(200212)344:10<1103::aid-adsc1103>3.0.co;2-r
    19 https://doi.org/10.1016/0141-0229(87)90136-0
    20 https://doi.org/10.1016/0141-0229(88)90062-2
    21 https://doi.org/10.1016/0141-0229(93)90053-5
    22 https://doi.org/10.1016/0957-4166(94)80100-2
    23 https://doi.org/10.1016/j.enzmictec.2006.05.004
    24 https://doi.org/10.1016/j.jbiotec.2005.03.015
    25 https://doi.org/10.1016/j.procbio.2004.06.060
    26 https://doi.org/10.1016/s0032-9592(02)00164-4
    27 https://doi.org/10.1016/s0040-4039(00)98627-1
    28 https://doi.org/10.1016/s0141-0229(98)00086-6
    29 https://doi.org/10.1016/s0168-1656(01)00359-5
    30 https://doi.org/10.1016/s0168-1656(97)00107-7
    31 https://doi.org/10.1016/s0378-4347(00)86033-8
    32 https://doi.org/10.1016/s0957-4166(98)00281-x
    33 https://doi.org/10.1021/ol026489d
    34 https://doi.org/10.1046/j.1432-1327.2000.00889.x
    35 https://doi.org/10.1055/s-1988-27577
    36 https://doi.org/10.1070/mc2003v013n03abeh001755
    37 https://doi.org/10.1111/j.1432-1033.1992.tb17300.x
    38 https://doi.org/10.1271/bbb.64.1255
    39 https://doi.org/10.1271/bbb1961.51.289
    40 https://doi.org/10.1271/bbb1961.51.3011
    41 schema:datePublished 2007-07
    42 schema:datePublishedReg 2007-07-01
    43 schema:description A fungus strain ECU2002, capable of enantioselectively hydrolyzing chiral lactones to optically pure hydroxy acids, was newly isolated from soil samples through two steps of screening and identified as Fusarium proliferatum (Matsushima) Nirenberg. From the crude extract of F. proliferatum ECU2002, a novel levo-lactonase was purified to homogeneity, with a purification factor of 460-folds and an overall yield of 9.7%, by ultrafiltration, acetone precipitation, and chromatographic separation through DEAE-Toyopearl, Butyl-Toyopearl, Hydroxyapatite, Toyoscreen-Super Q, and TSK-gel columns. The purified enzyme is a monomer; with a molecular mass of ca 68 kDa and a pI of 5.7 as determined by two-dimensional electrophoresis. The catalytic performance of the partially purified levo-lactonase was investigated, giving temperature and pH optima at 50 degrees C and 7.5, respectively, for gamma-butyrolactone hydrolysis. The substrate specificity of the partially purified lactonase was also examined using several useful lactones, among which alpha-hydroxy-gamma-butyrolactone was the best substrate, with 448-fold higher lactonase activity as compared to gamma-butyrolactone. The F. proliferatum lactonase preferentially hydrolyzed the levo enantiomer of butyrolactones, including beta-butyrolactone, alpha-hydroxy-gamma-butyrolactone, alpha-hydroxy-beta,beta-dimethyl-gamma-butyrolactone (pantolactone), and beta-hydroxy-gamma-butyrolactone, affording (+)-hydroxy acids in high (94.8 approximately 98.2%) enantiomeric excesses (ee) and good conversions (38.2 approximately 44.2%). A simple immobilization of the crude lactonase with glutaraldehyde cross-linking led to a stable and easy-to-handle biocatalyst for catalytic resolution of chiral lactones. The immobilized lactonase also performed quite well in repeated batch resolution of dl-pantolactone at a concentration of 35% (w/v), retaining 67% of initial activity after ten cycles of reaction (corresponding to a half life of 20 cycles) and affording the product in 94 approximately 97% ee, which can be easily enhanced to >99% ee after the d-hydroxy acid was chemically converted into l-lactone and crystallized.
    44 schema:genre research_article
    45 schema:inLanguage en
    46 schema:isAccessibleForFree false
    47 schema:isPartOf N57534e4dde194d3898288216082468af
    48 Nd28be4d9eb65463bb5f7a3ddf6a61a0e
    49 sg:journal.1083533
    50 schema:name Isolation and properties of a levo-lactonase from Fusarium proliferatum ECU2002: a robust biocatalyst for production of chiral lactones
    51 schema:pagination 1087-1094
    52 schema:productId N0845147dee8b477b9b95ccd0b3e70662
    53 N4089bac0405740aca730946db3aab1e3
    54 N608f5c9468434bbe8baef529d1e6508e
    55 Ncfd51e5b4ff542e79a12df379fbd3819
    56 Ne70e6f4b4b9a4e8cbb55783542829181
    57 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002209715
    58 https://doi.org/10.1007/s00253-007-0941-9
    59 schema:sdDatePublished 2019-04-10T21:36
    60 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    61 schema:sdPublisher N37857f19ade8438cac9863261811744c
    62 schema:url http://link.springer.com/10.1007%2Fs00253-007-0941-9
    63 sgo:license sg:explorer/license/
    64 sgo:sdDataset articles
    65 rdf:type schema:ScholarlyArticle
    66 N004627c68c104ba8bf3e739d37f17f26 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    67 schema:name Enzymes
    68 rdf:type schema:DefinedTerm
    69 N0845147dee8b477b9b95ccd0b3e70662 schema:name pubmed_id
    70 schema:value 17530243
    71 rdf:type schema:PropertyValue
    72 N37857f19ade8438cac9863261811744c schema:name Springer Nature - SN SciGraph project
    73 rdf:type schema:Organization
    74 N4089bac0405740aca730946db3aab1e3 schema:name doi
    75 schema:value 10.1007/s00253-007-0941-9
    76 rdf:type schema:PropertyValue
    77 N57534e4dde194d3898288216082468af schema:issueNumber 5
    78 rdf:type schema:PublicationIssue
    79 N608f5c9468434bbe8baef529d1e6508e schema:name nlm_unique_id
    80 schema:value 8406612
    81 rdf:type schema:PropertyValue
    82 N60fd1ab02b08405e88db81b8a11578f5 rdf:first sg:person.015623536651.64
    83 rdf:rest Ne42a9fc060e4490f8641f0984938bdc4
    84 N8e71d5312b30470e9d715fb72725da12 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    85 schema:name Lactones
    86 rdf:type schema:DefinedTerm
    87 Na96740cbf2814c9e9d25701e35890c56 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    88 schema:name Molecular Conformation
    89 rdf:type schema:DefinedTerm
    90 Nb32d73720471422cbb4583686a876500 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    91 schema:name Amino Acid Sequence
    92 rdf:type schema:DefinedTerm
    93 Nba71c74e463d4210baf6c3188b0acca2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    94 schema:name Molecular Sequence Data
    95 rdf:type schema:DefinedTerm
    96 Nc2acc9036ab34df0bffb5dbea08e81ae rdf:first sg:person.014401137627.32
    97 rdf:rest rdf:nil
    98 Ncfd51e5b4ff542e79a12df379fbd3819 schema:name readcube_id
    99 schema:value 0a85713429b5fbd053cc2e8ca41e8e0788936f06f57954a7fa96662f3be0e058
    100 rdf:type schema:PropertyValue
    101 Nd28be4d9eb65463bb5f7a3ddf6a61a0e schema:volumeNumber 75
    102 rdf:type schema:PublicationVolume
    103 Nd3336711e0c34ecb8777ab89488c3727 rdf:first sg:person.01365036241.45
    104 rdf:rest Nc2acc9036ab34df0bffb5dbea08e81ae
    105 Nd612490da0fe48519fbd659364ce9bc8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    106 schema:name Fusarium
    107 rdf:type schema:DefinedTerm
    108 Ne42a9fc060e4490f8641f0984938bdc4 rdf:first sg:person.01320335040.67
    109 rdf:rest Nd3336711e0c34ecb8777ab89488c3727
    110 Ne70e6f4b4b9a4e8cbb55783542829181 schema:name dimensions_id
    111 schema:value pub.1002209715
    112 rdf:type schema:PropertyValue
    113 anzsrc-for:03 schema:inDefinedTermSet anzsrc-for:
    114 schema:name Chemical Sciences
    115 rdf:type schema:DefinedTerm
    116 anzsrc-for:0306 schema:inDefinedTermSet anzsrc-for:
    117 schema:name Physical Chemistry (incl. Structural)
    118 rdf:type schema:DefinedTerm
    119 sg:journal.1083533 schema:issn 0175-7598
    120 1432-0614
    121 schema:name Applied Microbiology and Biotechnology
    122 rdf:type schema:Periodical
    123 sg:person.01320335040.67 schema:affiliation https://www.grid.ac/institutes/grid.28056.39
    124 schema:familyName Xu
    125 schema:givenName Jian-He
    126 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01320335040.67
    127 rdf:type schema:Person
    128 sg:person.01365036241.45 schema:affiliation https://www.grid.ac/institutes/grid.28056.39
    129 schema:familyName Xu
    130 schema:givenName Yi
    131 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01365036241.45
    132 rdf:type schema:Person
    133 sg:person.014401137627.32 schema:affiliation https://www.grid.ac/institutes/grid.28056.39
    134 schema:familyName Pan
    135 schema:givenName Jiang
    136 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014401137627.32
    137 rdf:type schema:Person
    138 sg:person.015623536651.64 schema:affiliation https://www.grid.ac/institutes/grid.28056.39
    139 schema:familyName Zhang
    140 schema:givenName Xian
    141 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015623536651.64
    142 rdf:type schema:Person
    143 sg:pub.10.1007/bf00169925 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044188776
    144 https://doi.org/10.1007/bf00169925
    145 rdf:type schema:CreativeWork
    146 sg:pub.10.1007/s00203-003-0597-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043429092
    147 https://doi.org/10.1007/s00203-003-0597-5
    148 rdf:type schema:CreativeWork
    149 sg:pub.10.1007/s002030100283 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031960956
    150 https://doi.org/10.1007/s002030100283
    151 rdf:type schema:CreativeWork
    152 https://app.dimensions.ai/details/publication/pub.1074890306 schema:CreativeWork
    153 https://app.dimensions.ai/details/publication/pub.1079943154 schema:CreativeWork
    154 https://app.dimensions.ai/details/publication/pub.1080459604 schema:CreativeWork
    155 https://app.dimensions.ai/details/publication/pub.1083382082 schema:CreativeWork
    156 https://doi.org/10.1002/(sici)1099-0690(199911)1999:11<2705::aid-ejoc2705>3.0.co;2-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049398507
    157 rdf:type schema:CreativeWork
    158 https://doi.org/10.1002/1615-4169(200212)344:10<1103::aid-adsc1103>3.0.co;2-r schema:sameAs https://app.dimensions.ai/details/publication/pub.1011355342
    159 rdf:type schema:CreativeWork
    160 https://doi.org/10.1016/0141-0229(87)90136-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029910220
    161 rdf:type schema:CreativeWork
    162 https://doi.org/10.1016/0141-0229(88)90062-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052458146
    163 rdf:type schema:CreativeWork
    164 https://doi.org/10.1016/0141-0229(93)90053-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037797630
    165 rdf:type schema:CreativeWork
    166 https://doi.org/10.1016/0957-4166(94)80100-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053732472
    167 rdf:type schema:CreativeWork
    168 https://doi.org/10.1016/j.enzmictec.2006.05.004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049039207
    169 rdf:type schema:CreativeWork
    170 https://doi.org/10.1016/j.jbiotec.2005.03.015 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017056527
    171 rdf:type schema:CreativeWork
    172 https://doi.org/10.1016/j.procbio.2004.06.060 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048850857
    173 rdf:type schema:CreativeWork
    174 https://doi.org/10.1016/s0032-9592(02)00164-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050220650
    175 rdf:type schema:CreativeWork
    176 https://doi.org/10.1016/s0040-4039(00)98627-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038115722
    177 rdf:type schema:CreativeWork
    178 https://doi.org/10.1016/s0141-0229(98)00086-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020862519
    179 rdf:type schema:CreativeWork
    180 https://doi.org/10.1016/s0168-1656(01)00359-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002659511
    181 rdf:type schema:CreativeWork
    182 https://doi.org/10.1016/s0168-1656(97)00107-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039031794
    183 rdf:type schema:CreativeWork
    184 https://doi.org/10.1016/s0378-4347(00)86033-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001783778
    185 rdf:type schema:CreativeWork
    186 https://doi.org/10.1016/s0957-4166(98)00281-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1018031031
    187 rdf:type schema:CreativeWork
    188 https://doi.org/10.1021/ol026489d schema:sameAs https://app.dimensions.ai/details/publication/pub.1056242983
    189 rdf:type schema:CreativeWork
    190 https://doi.org/10.1046/j.1432-1327.2000.00889.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1013683145
    191 rdf:type schema:CreativeWork
    192 https://doi.org/10.1055/s-1988-27577 schema:sameAs https://app.dimensions.ai/details/publication/pub.1057392192
    193 rdf:type schema:CreativeWork
    194 https://doi.org/10.1070/mc2003v013n03abeh001755 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033856978
    195 rdf:type schema:CreativeWork
    196 https://doi.org/10.1111/j.1432-1033.1992.tb17300.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1036433832
    197 rdf:type schema:CreativeWork
    198 https://doi.org/10.1271/bbb.64.1255 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006929833
    199 rdf:type schema:CreativeWork
    200 https://doi.org/10.1271/bbb1961.51.289 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028804364
    201 rdf:type schema:CreativeWork
    202 https://doi.org/10.1271/bbb1961.51.3011 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014838782
    203 rdf:type schema:CreativeWork
    204 https://www.grid.ac/institutes/grid.28056.39 schema:alternateName East China University of Science and Technology
    205 schema:name Laboratory of Biocatalysis and Bioprocessing, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, 200237, Shanghai, China
    206 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...