A highly productive system for cell-free protein synthesis using a lysate of the hyperthermophilic archaeon, Thermococcus kodakaraensis View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2007-04

AUTHORS

Takashi Endoh, Tamotsu Kanai, Tadayuki Imanaka

ABSTRACT

We report in this study an improved system for cell-free protein synthesis at high temperatures using the lysate of Thermococcus kodakaraensis. Previous work indicated that cell-free protein synthesis of ChiADelta4, a derivative of T. kodakaraensis chitinase, was observed within a temperature range of 40-80 degrees C, and the maximum yield of the ChiADelta4 synthesized was approximately 1.3 microg/ml. To increase productivity of the system, the following approaches were taken. First, the process of lysate preparation was examined, and we found that omitting the preincubation (runoff) step was especially effective to increase the translational activity of lysate. Second, the concentrations of each reaction mixture were optimized. Among them, the requirement of a high concentration of potassium acetate (250 mM) was characteristic to the T. kodakaraensis system. Third, a mutant strain of T. kodakaraensis was constructed in which a heat shock transcriptional regulator gene, phr, was disrupted. By using the lysate made from the mutant, we observed an increase in the optimum reaction temperature by 5 degrees C. Through these modifications to the system, the yield of ChiADelta4 was dramatically increased to 115.4 microg/ml in a batch reaction at 65 degrees C, which was about 90 times higher than that in the previous study. Moreover, in the optimized system, a high speed of protein synthesis was achieved: over 100 microg/ml of ChiADelta4 was produced in the first 15 min of reaction. These results indicate that the system for cell-free protein synthesis based on T. kodakaraensis lysate has a high production potential comparable to the Escherichia coli system. More... »

PAGES

1153

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00253-006-0753-3

DOI

http://dx.doi.org/10.1007/s00253-006-0753-3

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1017764121

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/17165083


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cloning, Molecular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Recombinant Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Thermococcus", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Kyoto University", 
          "id": "https://www.grid.ac/institutes/grid.258799.8", 
          "name": [
            "Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, 615-8510, Kyoto, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Endoh", 
        "givenName": "Takashi", 
        "id": "sg:person.01204236151.18", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01204236151.18"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Kyoto University", 
          "id": "https://www.grid.ac/institutes/grid.258799.8", 
          "name": [
            "Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, 615-8510, Kyoto, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kanai", 
        "givenName": "Tamotsu", 
        "id": "sg:person.0721605175.45", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0721605175.45"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Kyoto University", 
          "id": "https://www.grid.ac/institutes/grid.258799.8", 
          "name": [
            "Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, 615-8510, Kyoto, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Imanaka", 
        "givenName": "Tadayuki", 
        "id": "sg:person.0700462536.36", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0700462536.36"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1128/jb.183.17.5198-5202.2001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000260097"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s1389-1723(02)80007-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000582966"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/bit.10827", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003278247"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/(sici)1097-0290(1999)66:3<180::aid-bit6>3.0.co;2-s", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004069852"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0734-9750(03)00105-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004330248"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0734-9750(03)00105-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004330248"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bp049789y", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004425623"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0065-3233(08)60368-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005859023"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/bit.20831", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008399070"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s007920100236", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008980210", 
          "https://doi.org/10.1007/s007920100236"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0168-1656(96)00022-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010727113"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1006/abio.1995.1231", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012572883"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.185.1.210-220.2003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019365440"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jbiotec.2004.11.002", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020703047"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1155/2004/204953", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020943443"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s002530000534", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021166857", 
          "https://doi.org/10.1007/s002530000534"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jbiotec.2005.12.030", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022801612"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jbiotec.2006.04.010", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026336917"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0014-5793(92)81008-a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028147251"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.95.2.520", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035550341"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m209250200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036930458"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0014-5793(98)01620-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041832086"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/s007920050050", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042547180", 
          "https://doi.org/10.1007/s007920050050"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/aem.71.7.3889-3899.2005", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042656779"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0168-1656(00)00326-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044853028"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gr.3003105", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047686511"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.jbiotec.2006.05.014", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052096266"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074500430", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1074557751", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1077202572", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082518511", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2007-04", 
    "datePublishedReg": "2007-04-01", 
    "description": "We report in this study an improved system for cell-free protein synthesis at high temperatures using the lysate of Thermococcus kodakaraensis. Previous work indicated that cell-free protein synthesis of ChiADelta4, a derivative of T. kodakaraensis chitinase, was observed within a temperature range of 40-80 degrees C, and the maximum yield of the ChiADelta4 synthesized was approximately 1.3 microg/ml. To increase productivity of the system, the following approaches were taken. First, the process of lysate preparation was examined, and we found that omitting the preincubation (runoff) step was especially effective to increase the translational activity of lysate. Second, the concentrations of each reaction mixture were optimized. Among them, the requirement of a high concentration of potassium acetate (250 mM) was characteristic to the T. kodakaraensis system. Third, a mutant strain of T. kodakaraensis was constructed in which a heat shock transcriptional regulator gene, phr, was disrupted. By using the lysate made from the mutant, we observed an increase in the optimum reaction temperature by 5 degrees C. Through these modifications to the system, the yield of ChiADelta4 was dramatically increased to 115.4 microg/ml in a batch reaction at 65 degrees C, which was about 90 times higher than that in the previous study. Moreover, in the optimized system, a high speed of protein synthesis was achieved: over 100 microg/ml of ChiADelta4 was produced in the first 15 min of reaction. These results indicate that the system for cell-free protein synthesis based on T. kodakaraensis lysate has a high production potential comparable to the Escherichia coli system.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s00253-006-0753-3", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1083533", 
        "issn": [
          "0175-7598", 
          "1432-0614"
        ], 
        "name": "Applied Microbiology and Biotechnology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "5", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "74"
      }
    ], 
    "name": "A highly productive system for cell-free protein synthesis using a lysate of the hyperthermophilic archaeon, Thermococcus kodakaraensis", 
    "pagination": "1153", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "54a3ca5495985ab00f98509beffccb7991b33e5a7c2eb1145295d94810869132"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "17165083"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "8406612"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00253-006-0753-3"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1017764121"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00253-006-0753-3", 
      "https://app.dimensions.ai/details/publication/pub.1017764121"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T14:25", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000373_0000000373/records_13068_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/s00253-006-0753-3"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00253-006-0753-3'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00253-006-0753-3'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00253-006-0753-3'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00253-006-0753-3'


 

This table displays all metadata directly associated to this object as RDF triples.

184 TRIPLES      21 PREDICATES      62 URIs      24 LITERALS      12 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00253-006-0753-3 schema:about N8145f3a5e63d42c0aefd8128360402b3
2 Nb088f27a45aa4ccda94d9a774af43a42
3 Ne3a327ecbcc94b63a630ad3860e70d90
4 anzsrc-for:06
5 anzsrc-for:0601
6 schema:author N92befed191c14f57ba87992a17c0a37b
7 schema:citation sg:pub.10.1007/s002530000534
8 sg:pub.10.1007/s007920050050
9 sg:pub.10.1007/s007920100236
10 https://app.dimensions.ai/details/publication/pub.1074500430
11 https://app.dimensions.ai/details/publication/pub.1074557751
12 https://app.dimensions.ai/details/publication/pub.1077202572
13 https://app.dimensions.ai/details/publication/pub.1082518511
14 https://doi.org/10.1002/(sici)1097-0290(1999)66:3<180::aid-bit6>3.0.co;2-s
15 https://doi.org/10.1002/bit.10827
16 https://doi.org/10.1002/bit.20831
17 https://doi.org/10.1006/abio.1995.1231
18 https://doi.org/10.1016/0014-5793(92)81008-a
19 https://doi.org/10.1016/0168-1656(96)00022-3
20 https://doi.org/10.1016/j.jbiotec.2004.11.002
21 https://doi.org/10.1016/j.jbiotec.2005.12.030
22 https://doi.org/10.1016/j.jbiotec.2006.04.010
23 https://doi.org/10.1016/j.jbiotec.2006.05.014
24 https://doi.org/10.1016/s0014-5793(98)01620-2
25 https://doi.org/10.1016/s0065-3233(08)60368-x
26 https://doi.org/10.1016/s0168-1656(00)00326-6
27 https://doi.org/10.1016/s0734-9750(03)00105-8
28 https://doi.org/10.1016/s1389-1723(02)80007-x
29 https://doi.org/10.1021/bp049789y
30 https://doi.org/10.1073/pnas.95.2.520
31 https://doi.org/10.1074/jbc.m209250200
32 https://doi.org/10.1101/gr.3003105
33 https://doi.org/10.1128/aem.71.7.3889-3899.2005
34 https://doi.org/10.1128/jb.183.17.5198-5202.2001
35 https://doi.org/10.1128/jb.185.1.210-220.2003
36 https://doi.org/10.1155/2004/204953
37 schema:datePublished 2007-04
38 schema:datePublishedReg 2007-04-01
39 schema:description We report in this study an improved system for cell-free protein synthesis at high temperatures using the lysate of Thermococcus kodakaraensis. Previous work indicated that cell-free protein synthesis of ChiADelta4, a derivative of T. kodakaraensis chitinase, was observed within a temperature range of 40-80 degrees C, and the maximum yield of the ChiADelta4 synthesized was approximately 1.3 microg/ml. To increase productivity of the system, the following approaches were taken. First, the process of lysate preparation was examined, and we found that omitting the preincubation (runoff) step was especially effective to increase the translational activity of lysate. Second, the concentrations of each reaction mixture were optimized. Among them, the requirement of a high concentration of potassium acetate (250 mM) was characteristic to the T. kodakaraensis system. Third, a mutant strain of T. kodakaraensis was constructed in which a heat shock transcriptional regulator gene, phr, was disrupted. By using the lysate made from the mutant, we observed an increase in the optimum reaction temperature by 5 degrees C. Through these modifications to the system, the yield of ChiADelta4 was dramatically increased to 115.4 microg/ml in a batch reaction at 65 degrees C, which was about 90 times higher than that in the previous study. Moreover, in the optimized system, a high speed of protein synthesis was achieved: over 100 microg/ml of ChiADelta4 was produced in the first 15 min of reaction. These results indicate that the system for cell-free protein synthesis based on T. kodakaraensis lysate has a high production potential comparable to the Escherichia coli system.
40 schema:genre research_article
41 schema:inLanguage en
42 schema:isAccessibleForFree false
43 schema:isPartOf N1bef5565b1bf478caccbb78b1100eb19
44 Nc7e62c859e474082b4f720f2970376d2
45 sg:journal.1083533
46 schema:name A highly productive system for cell-free protein synthesis using a lysate of the hyperthermophilic archaeon, Thermococcus kodakaraensis
47 schema:pagination 1153
48 schema:productId N00e1e7194c134ee4be5d42d15824968b
49 N3df2a7f420454b06a7b190c7c17e56ce
50 N933511aa6efb460e8fec0b09437807e8
51 N9e5f149378074ed682ef5e6dff1c61f9
52 Nf10364955b4b4e4fbce2be8d0552e5c6
53 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017764121
54 https://doi.org/10.1007/s00253-006-0753-3
55 schema:sdDatePublished 2019-04-11T14:25
56 schema:sdLicense https://scigraph.springernature.com/explorer/license/
57 schema:sdPublisher N90dd4079be154794a870527a4b30fba7
58 schema:url http://link.springer.com/10.1007/s00253-006-0753-3
59 sgo:license sg:explorer/license/
60 sgo:sdDataset articles
61 rdf:type schema:ScholarlyArticle
62 N00e1e7194c134ee4be5d42d15824968b schema:name pubmed_id
63 schema:value 17165083
64 rdf:type schema:PropertyValue
65 N09a9c22655b347b581131d6ebb9b76e5 rdf:first sg:person.0721605175.45
66 rdf:rest Ndfb981e43ba347869694c10797df769e
67 N1bef5565b1bf478caccbb78b1100eb19 schema:volumeNumber 74
68 rdf:type schema:PublicationVolume
69 N3df2a7f420454b06a7b190c7c17e56ce schema:name readcube_id
70 schema:value 54a3ca5495985ab00f98509beffccb7991b33e5a7c2eb1145295d94810869132
71 rdf:type schema:PropertyValue
72 N8145f3a5e63d42c0aefd8128360402b3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
73 schema:name Thermococcus
74 rdf:type schema:DefinedTerm
75 N90dd4079be154794a870527a4b30fba7 schema:name Springer Nature - SN SciGraph project
76 rdf:type schema:Organization
77 N92befed191c14f57ba87992a17c0a37b rdf:first sg:person.01204236151.18
78 rdf:rest N09a9c22655b347b581131d6ebb9b76e5
79 N933511aa6efb460e8fec0b09437807e8 schema:name nlm_unique_id
80 schema:value 8406612
81 rdf:type schema:PropertyValue
82 N9e5f149378074ed682ef5e6dff1c61f9 schema:name doi
83 schema:value 10.1007/s00253-006-0753-3
84 rdf:type schema:PropertyValue
85 Nb088f27a45aa4ccda94d9a774af43a42 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
86 schema:name Cloning, Molecular
87 rdf:type schema:DefinedTerm
88 Nc7e62c859e474082b4f720f2970376d2 schema:issueNumber 5
89 rdf:type schema:PublicationIssue
90 Ndfb981e43ba347869694c10797df769e rdf:first sg:person.0700462536.36
91 rdf:rest rdf:nil
92 Ne3a327ecbcc94b63a630ad3860e70d90 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name Recombinant Proteins
94 rdf:type schema:DefinedTerm
95 Nf10364955b4b4e4fbce2be8d0552e5c6 schema:name dimensions_id
96 schema:value pub.1017764121
97 rdf:type schema:PropertyValue
98 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
99 schema:name Biological Sciences
100 rdf:type schema:DefinedTerm
101 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
102 schema:name Biochemistry and Cell Biology
103 rdf:type schema:DefinedTerm
104 sg:journal.1083533 schema:issn 0175-7598
105 1432-0614
106 schema:name Applied Microbiology and Biotechnology
107 rdf:type schema:Periodical
108 sg:person.01204236151.18 schema:affiliation https://www.grid.ac/institutes/grid.258799.8
109 schema:familyName Endoh
110 schema:givenName Takashi
111 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01204236151.18
112 rdf:type schema:Person
113 sg:person.0700462536.36 schema:affiliation https://www.grid.ac/institutes/grid.258799.8
114 schema:familyName Imanaka
115 schema:givenName Tadayuki
116 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0700462536.36
117 rdf:type schema:Person
118 sg:person.0721605175.45 schema:affiliation https://www.grid.ac/institutes/grid.258799.8
119 schema:familyName Kanai
120 schema:givenName Tamotsu
121 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0721605175.45
122 rdf:type schema:Person
123 sg:pub.10.1007/s002530000534 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021166857
124 https://doi.org/10.1007/s002530000534
125 rdf:type schema:CreativeWork
126 sg:pub.10.1007/s007920050050 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042547180
127 https://doi.org/10.1007/s007920050050
128 rdf:type schema:CreativeWork
129 sg:pub.10.1007/s007920100236 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008980210
130 https://doi.org/10.1007/s007920100236
131 rdf:type schema:CreativeWork
132 https://app.dimensions.ai/details/publication/pub.1074500430 schema:CreativeWork
133 https://app.dimensions.ai/details/publication/pub.1074557751 schema:CreativeWork
134 https://app.dimensions.ai/details/publication/pub.1077202572 schema:CreativeWork
135 https://app.dimensions.ai/details/publication/pub.1082518511 schema:CreativeWork
136 https://doi.org/10.1002/(sici)1097-0290(1999)66:3<180::aid-bit6>3.0.co;2-s schema:sameAs https://app.dimensions.ai/details/publication/pub.1004069852
137 rdf:type schema:CreativeWork
138 https://doi.org/10.1002/bit.10827 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003278247
139 rdf:type schema:CreativeWork
140 https://doi.org/10.1002/bit.20831 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008399070
141 rdf:type schema:CreativeWork
142 https://doi.org/10.1006/abio.1995.1231 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012572883
143 rdf:type schema:CreativeWork
144 https://doi.org/10.1016/0014-5793(92)81008-a schema:sameAs https://app.dimensions.ai/details/publication/pub.1028147251
145 rdf:type schema:CreativeWork
146 https://doi.org/10.1016/0168-1656(96)00022-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010727113
147 rdf:type schema:CreativeWork
148 https://doi.org/10.1016/j.jbiotec.2004.11.002 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020703047
149 rdf:type schema:CreativeWork
150 https://doi.org/10.1016/j.jbiotec.2005.12.030 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022801612
151 rdf:type schema:CreativeWork
152 https://doi.org/10.1016/j.jbiotec.2006.04.010 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026336917
153 rdf:type schema:CreativeWork
154 https://doi.org/10.1016/j.jbiotec.2006.05.014 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052096266
155 rdf:type schema:CreativeWork
156 https://doi.org/10.1016/s0014-5793(98)01620-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041832086
157 rdf:type schema:CreativeWork
158 https://doi.org/10.1016/s0065-3233(08)60368-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1005859023
159 rdf:type schema:CreativeWork
160 https://doi.org/10.1016/s0168-1656(00)00326-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044853028
161 rdf:type schema:CreativeWork
162 https://doi.org/10.1016/s0734-9750(03)00105-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004330248
163 rdf:type schema:CreativeWork
164 https://doi.org/10.1016/s1389-1723(02)80007-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1000582966
165 rdf:type schema:CreativeWork
166 https://doi.org/10.1021/bp049789y schema:sameAs https://app.dimensions.ai/details/publication/pub.1004425623
167 rdf:type schema:CreativeWork
168 https://doi.org/10.1073/pnas.95.2.520 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035550341
169 rdf:type schema:CreativeWork
170 https://doi.org/10.1074/jbc.m209250200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036930458
171 rdf:type schema:CreativeWork
172 https://doi.org/10.1101/gr.3003105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047686511
173 rdf:type schema:CreativeWork
174 https://doi.org/10.1128/aem.71.7.3889-3899.2005 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042656779
175 rdf:type schema:CreativeWork
176 https://doi.org/10.1128/jb.183.17.5198-5202.2001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000260097
177 rdf:type schema:CreativeWork
178 https://doi.org/10.1128/jb.185.1.210-220.2003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019365440
179 rdf:type schema:CreativeWork
180 https://doi.org/10.1155/2004/204953 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020943443
181 rdf:type schema:CreativeWork
182 https://www.grid.ac/institutes/grid.258799.8 schema:alternateName Kyoto University
183 schema:name Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Katsura, Nishikyo-ku, 615-8510, Kyoto, Japan
184 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...