Bioconversion of butyronitrile to butyramide using whole cells of Rhodococcus rhodochrous PA-34 View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2007-01-11

AUTHORS

J. Raj, A. Seth, S. Prasad, T. C. Bhalla

ABSTRACT

Butyramide is an important chemical commodity, which is used for the synthesis of hydroxamic acids and electrorheological fluids and for the preparation of β-amodoorganotin compounds. The nitrile hydratase (Nhase) of Rhodococcus rhodochrous PA-34 catalyzed the conversion of butyronitrile to butyramide. The maximum Nhase activity [18 U/mg dry cell weight (dcw)] of whole cells of R. rhodochrous PA-34 was observed at pH 7.0 with 10% (v/v) butyronitrile and 1 mg cells (dcw)/ml reaction mixture at 10°C. The cells of R. rhodochrous PA-34 retained almost 50% activity when incubated for 1 h in the presence of 85% (v/v) butyronitrile. A yield of 597 g of butyramide (6.8 M) was obtained using 60% (v/v) butyronitrile, 1 g cells (dry weight) in a 1-l batch reaction at 10°C for 6 h. More... »

PAGES

535-539

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00253-006-0693-y

DOI

http://dx.doi.org/10.1007/s00253-006-0693-y

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1001633005

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/17216468


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/03", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Chemical Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0305", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Organic Chemistry", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amides", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Biotransformation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hydro-Lyases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hydrogen-Ion Concentration", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Nitriles", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Rhodococcus", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Temperature", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Time Factors", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Biotechnology, Himachal Pradesh University, Summer Hill, 171 005, Shimla, India", 
          "id": "http://www.grid.ac/institutes/grid.412137.2", 
          "name": [
            "Department of Biotechnology, Himachal Pradesh University, Summer Hill, 171 005, Shimla, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Raj", 
        "givenName": "J.", 
        "id": "sg:person.0751537315.09", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0751537315.09"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Biotechnology, Himachal Pradesh University, Summer Hill, 171 005, Shimla, India", 
          "id": "http://www.grid.ac/institutes/grid.412137.2", 
          "name": [
            "Department of Biotechnology, Himachal Pradesh University, Summer Hill, 171 005, Shimla, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Seth", 
        "givenName": "A.", 
        "id": "sg:person.0663615620.14", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0663615620.14"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Biotechnology, Himachal Pradesh University, Summer Hill, 171 005, Shimla, India", 
          "id": "http://www.grid.ac/institutes/grid.412137.2", 
          "name": [
            "Department of Biotechnology, Himachal Pradesh University, Summer Hill, 171 005, Shimla, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Prasad", 
        "givenName": "S.", 
        "id": "sg:person.01014373151.85", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01014373151.85"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Department of Biotechnology, Himachal Pradesh University, Summer Hill, 171 005, Shimla, India", 
          "id": "http://www.grid.ac/institutes/grid.412137.2", 
          "name": [
            "Department of Biotechnology, Himachal Pradesh University, Summer Hill, 171 005, Shimla, India"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bhalla", 
        "givenName": "T. C.", 
        "id": "sg:person.01354154245.05", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01354154245.05"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/bf00170364", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034176166", 
          "https://doi.org/10.1007/bf00170364"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00169350", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050870921", 
          "https://doi.org/10.1007/bf00169350"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1023/b:bile.0000045636.47527.99", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038145871", 
          "https://doi.org/10.1023/b:bile.0000045636.47527.99"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00178168", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049966309", 
          "https://doi.org/10.1007/bf00178168"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2007-01-11", 
    "datePublishedReg": "2007-01-11", 
    "description": "Butyramide is an important chemical commodity, which is used for the synthesis of hydroxamic acids and electrorheological fluids and for the preparation of \u03b2-amodoorganotin compounds. The nitrile hydratase (Nhase) of Rhodococcus rhodochrous PA-34 catalyzed the conversion of butyronitrile to butyramide. The maximum Nhase activity [18\u00a0U/mg dry cell weight (dcw)] of whole cells of R. rhodochrous PA-34 was observed at pH\u00a07.0 with 10% (v/v) butyronitrile and 1\u00a0mg cells (dcw)/ml reaction mixture at 10\u00b0C. The cells of R. rhodochrous PA-34 retained almost 50% activity when incubated for 1\u00a0h in the presence of 85% (v/v) butyronitrile. A yield of 597\u00a0g of butyramide (6.8\u00a0M) was obtained using 60% (v/v) butyronitrile, 1\u00a0g cells (dry weight) in a 1-l batch reaction at 10\u00b0C for 6\u00a0h.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/s00253-006-0693-y", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1083533", 
        "issn": [
          "0175-7598", 
          "1432-0614"
        ], 
        "name": "Applied Microbiology and Biotechnology", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "3", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "74"
      }
    ], 
    "keywords": [
      "important chemical commodity", 
      "maximum NHase activity", 
      "chemical commodities", 
      "reaction mixture", 
      "butyronitrile", 
      "batch reactions", 
      "rhodochrous PA-34", 
      "hydroxamic acid", 
      "nitrile hydratase", 
      "Rhodococcus rhodochrous PA-34", 
      "whole cells", 
      "electrorheological fluids", 
      "butyramide", 
      "synthesis", 
      "compounds", 
      "reaction", 
      "preparation", 
      "mixture", 
      "NHase activity", 
      "conversion", 
      "acid", 
      "yield", 
      "hydratase", 
      "activity", 
      "presence", 
      "bioconversion", 
      "cells", 
      "fluid", 
      "commodities"
    ], 
    "name": "Bioconversion of butyronitrile to butyramide using whole cells of Rhodococcus rhodochrous PA-34", 
    "pagination": "535-539", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1001633005"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00253-006-0693-y"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "17216468"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00253-006-0693-y", 
      "https://app.dimensions.ai/details/publication/pub.1001633005"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-12-01T06:26", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20221201/entities/gbq_results/article/article_435.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/s00253-006-0693-y"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00253-006-0693-y'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00253-006-0693-y'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00253-006-0693-y'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00253-006-0693-y'


 

This table displays all metadata directly associated to this object as RDF triples.

159 TRIPLES      21 PREDICATES      66 URIs      54 LITERALS      15 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00253-006-0693-y schema:about N15a6d39835db4817b326232b296cd2cd
2 N1a74d2e7e12b4dc58ce2cd8b8e4c52b3
3 N2212b50ead4c4d4e9178ba838ee944e9
4 N904b274fd60843138c33aa28ba6729aa
5 N908a030fe90744b0898b94efddb402fd
6 Na625b6c31acb459c9a62fd6a76aa670b
7 Ncbe7b2533a264e9f998daa41c2d42b6d
8 Nde79c7b0451b44f0a4d9f6dff314a57c
9 anzsrc-for:03
10 anzsrc-for:0305
11 schema:author Naedb5a1529054010b3488b6fe2c2e50b
12 schema:citation sg:pub.10.1007/bf00169350
13 sg:pub.10.1007/bf00170364
14 sg:pub.10.1007/bf00178168
15 sg:pub.10.1023/b:bile.0000045636.47527.99
16 schema:datePublished 2007-01-11
17 schema:datePublishedReg 2007-01-11
18 schema:description Butyramide is an important chemical commodity, which is used for the synthesis of hydroxamic acids and electrorheological fluids and for the preparation of β-amodoorganotin compounds. The nitrile hydratase (Nhase) of Rhodococcus rhodochrous PA-34 catalyzed the conversion of butyronitrile to butyramide. The maximum Nhase activity [18 U/mg dry cell weight (dcw)] of whole cells of R. rhodochrous PA-34 was observed at pH 7.0 with 10% (v/v) butyronitrile and 1 mg cells (dcw)/ml reaction mixture at 10°C. The cells of R. rhodochrous PA-34 retained almost 50% activity when incubated for 1 h in the presence of 85% (v/v) butyronitrile. A yield of 597 g of butyramide (6.8 M) was obtained using 60% (v/v) butyronitrile, 1 g cells (dry weight) in a 1-l batch reaction at 10°C for 6 h.
19 schema:genre article
20 schema:isAccessibleForFree false
21 schema:isPartOf N0e4ac10ac4ce4e3da881d39592f0b126
22 N38a841ea770f4703b16451e2be4a9e27
23 sg:journal.1083533
24 schema:keywords NHase activity
25 Rhodococcus rhodochrous PA-34
26 acid
27 activity
28 batch reactions
29 bioconversion
30 butyramide
31 butyronitrile
32 cells
33 chemical commodities
34 commodities
35 compounds
36 conversion
37 electrorheological fluids
38 fluid
39 hydratase
40 hydroxamic acid
41 important chemical commodity
42 maximum NHase activity
43 mixture
44 nitrile hydratase
45 preparation
46 presence
47 reaction
48 reaction mixture
49 rhodochrous PA-34
50 synthesis
51 whole cells
52 yield
53 schema:name Bioconversion of butyronitrile to butyramide using whole cells of Rhodococcus rhodochrous PA-34
54 schema:pagination 535-539
55 schema:productId N30776d8f041c45bba6075d0ef6d4f4e9
56 N79298f536a574e89934c1a5cb5553880
57 Nfdb32394b83f42ed837face6093dc5b8
58 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001633005
59 https://doi.org/10.1007/s00253-006-0693-y
60 schema:sdDatePublished 2022-12-01T06:26
61 schema:sdLicense https://scigraph.springernature.com/explorer/license/
62 schema:sdPublisher Nd9be69e72d3d457b93811fe695e7387a
63 schema:url https://doi.org/10.1007/s00253-006-0693-y
64 sgo:license sg:explorer/license/
65 sgo:sdDataset articles
66 rdf:type schema:ScholarlyArticle
67 N0e4ac10ac4ce4e3da881d39592f0b126 schema:issueNumber 3
68 rdf:type schema:PublicationIssue
69 N14968ee83f8d444eb77e76ed734ac340 rdf:first sg:person.01014373151.85
70 rdf:rest N758b1dce87a6415398c45f10332db16c
71 N15a6d39835db4817b326232b296cd2cd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
72 schema:name Biotransformation
73 rdf:type schema:DefinedTerm
74 N1a74d2e7e12b4dc58ce2cd8b8e4c52b3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
75 schema:name Amides
76 rdf:type schema:DefinedTerm
77 N2212b50ead4c4d4e9178ba838ee944e9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
78 schema:name Rhodococcus
79 rdf:type schema:DefinedTerm
80 N30776d8f041c45bba6075d0ef6d4f4e9 schema:name pubmed_id
81 schema:value 17216468
82 rdf:type schema:PropertyValue
83 N38a841ea770f4703b16451e2be4a9e27 schema:volumeNumber 74
84 rdf:type schema:PublicationVolume
85 N758b1dce87a6415398c45f10332db16c rdf:first sg:person.01354154245.05
86 rdf:rest rdf:nil
87 N79298f536a574e89934c1a5cb5553880 schema:name doi
88 schema:value 10.1007/s00253-006-0693-y
89 rdf:type schema:PropertyValue
90 N8aeec366cee14973a42b232474d197e2 rdf:first sg:person.0663615620.14
91 rdf:rest N14968ee83f8d444eb77e76ed734ac340
92 N904b274fd60843138c33aa28ba6729aa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name Hydro-Lyases
94 rdf:type schema:DefinedTerm
95 N908a030fe90744b0898b94efddb402fd schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
96 schema:name Hydrogen-Ion Concentration
97 rdf:type schema:DefinedTerm
98 Na625b6c31acb459c9a62fd6a76aa670b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Nitriles
100 rdf:type schema:DefinedTerm
101 Naedb5a1529054010b3488b6fe2c2e50b rdf:first sg:person.0751537315.09
102 rdf:rest N8aeec366cee14973a42b232474d197e2
103 Ncbe7b2533a264e9f998daa41c2d42b6d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
104 schema:name Temperature
105 rdf:type schema:DefinedTerm
106 Nd9be69e72d3d457b93811fe695e7387a schema:name Springer Nature - SN SciGraph project
107 rdf:type schema:Organization
108 Nde79c7b0451b44f0a4d9f6dff314a57c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
109 schema:name Time Factors
110 rdf:type schema:DefinedTerm
111 Nfdb32394b83f42ed837face6093dc5b8 schema:name dimensions_id
112 schema:value pub.1001633005
113 rdf:type schema:PropertyValue
114 anzsrc-for:03 schema:inDefinedTermSet anzsrc-for:
115 schema:name Chemical Sciences
116 rdf:type schema:DefinedTerm
117 anzsrc-for:0305 schema:inDefinedTermSet anzsrc-for:
118 schema:name Organic Chemistry
119 rdf:type schema:DefinedTerm
120 sg:journal.1083533 schema:issn 0175-7598
121 1432-0614
122 schema:name Applied Microbiology and Biotechnology
123 schema:publisher Springer Nature
124 rdf:type schema:Periodical
125 sg:person.01014373151.85 schema:affiliation grid-institutes:grid.412137.2
126 schema:familyName Prasad
127 schema:givenName S.
128 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01014373151.85
129 rdf:type schema:Person
130 sg:person.01354154245.05 schema:affiliation grid-institutes:grid.412137.2
131 schema:familyName Bhalla
132 schema:givenName T. C.
133 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01354154245.05
134 rdf:type schema:Person
135 sg:person.0663615620.14 schema:affiliation grid-institutes:grid.412137.2
136 schema:familyName Seth
137 schema:givenName A.
138 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0663615620.14
139 rdf:type schema:Person
140 sg:person.0751537315.09 schema:affiliation grid-institutes:grid.412137.2
141 schema:familyName Raj
142 schema:givenName J.
143 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0751537315.09
144 rdf:type schema:Person
145 sg:pub.10.1007/bf00169350 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050870921
146 https://doi.org/10.1007/bf00169350
147 rdf:type schema:CreativeWork
148 sg:pub.10.1007/bf00170364 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034176166
149 https://doi.org/10.1007/bf00170364
150 rdf:type schema:CreativeWork
151 sg:pub.10.1007/bf00178168 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049966309
152 https://doi.org/10.1007/bf00178168
153 rdf:type schema:CreativeWork
154 sg:pub.10.1023/b:bile.0000045636.47527.99 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038145871
155 https://doi.org/10.1023/b:bile.0000045636.47527.99
156 rdf:type schema:CreativeWork
157 grid-institutes:grid.412137.2 schema:alternateName Department of Biotechnology, Himachal Pradesh University, Summer Hill, 171 005, Shimla, India
158 schema:name Department of Biotechnology, Himachal Pradesh University, Summer Hill, 171 005, Shimla, India
159 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...