Purification, cloning, sequencing and over-expression in Escherichia coli of a regioselective aliphatic nitrilase from Acidovorax facilis 72W View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2003-01-16

AUTHORS

S. Chauhan, S. Wu, S. Blumerman, R. D. Fallon, J. E. Gavagan, R. DiCosimo, M. S. Payne

ABSTRACT

A regioselective aliphatic nitrilase from Acidovorax facilis 72W was purified and characterized, and the corresponding gene was cloned and sequenced. This nitrilase gene was over-expressed in Escherichia coli, generating a microorganism that efficiently and regioselectively catalyzes the conversion of aliphatic dinitriles to cyanocarboxylic acids. The high yields obtained, mild reaction conditions used, and robustness observed make this biocatalyst suitable for industrial applications. More... »

PAGES

118-122

Journal

TITLE

Applied Microbiology and Biotechnology

ISSUE

2

VOLUME

61

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00253-002-1192-4

    DOI

    http://dx.doi.org/10.1007/s00253-002-1192-4

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1048755176

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/12655453


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