Importance of redox balance on the production of succinic acid by metabolically engineered Escherichia coli View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2002-03

AUTHORS

S. Hong, S. Lee

ABSTRACT

We had previously shown that succinic acid production in a pfl ldhA double mutant strain of Escherichia coli could be enhanced by amplifying the malic enzyme activity. However, recombinant E. coli NZN111 (F- Apfl::Cam ldhA::Kan) harboring pTrcML, a plasmid containing the E. coli malic enzyme gene, produced a considerable amount of malic acid along with the desired product, succinic acid. To have an insight into the intracellular metabolism, metabolic control analysis was carried out. From the results of a simulation, it was predicted that supplying additional reducing power could enhance succinic acid production. More reduced carbon substrate sorbitol was thus examined for the possibility of matching the potential during succinic acid production. When NZN111 (pTrcML) was cultured in LB medium containing 20 g sorbitol/l under a CO2 atmosphere, 10 g succinic acid/l was produced. The apparent yield of succinic acid was 1.1 g succinic acid/g sorbitol, which is 85% of the maximum theoretical yield. Therefore, it was found that redox balancing was important for the enhanced production of succinic acid in metabolically engineered E. coli. More... »

PAGES

286-290

Journal

TITLE

Applied Microbiology and Biotechnology

ISSUE

3

VOLUME

58

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00253-001-0899-y

DOI

http://dx.doi.org/10.1007/s00253-001-0899-y

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1037208698

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/11935177


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0605", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Microbiology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Carbon Dioxide", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Culture Media", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Escherichia coli", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fermentation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Genetic Engineering", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Indicators and Reagents", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Malates", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Oxidation-Reduction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sorbitol", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Succinic Acid", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Korea Advanced Institute of Science and Technology", 
          "id": "https://www.grid.ac/institutes/grid.37172.30", 
          "name": [
            "Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, 373-1 Kusong-dong, Yusong-gu, Taejon 305-701, Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Hong", 
        "givenName": "S.", 
        "id": "sg:person.01067521611.84", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01067521611.84"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Korea Advanced Institute of Science and Technology", 
          "id": "https://www.grid.ac/institutes/grid.37172.30", 
          "name": [
            "Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, 373-1 Kusong-dong, Yusong-gu, Taejon 305-701, Korea"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lee", 
        "givenName": "S.", 
        "id": "sg:person.01111321475.48", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01111321475.48"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "2002-03", 
    "datePublishedReg": "2002-03-01", 
    "description": "We had previously shown that succinic acid production in a pfl ldhA double mutant strain of Escherichia coli could be enhanced by amplifying the malic enzyme activity. However, recombinant E. coli NZN111 (F- Apfl::Cam ldhA::Kan) harboring pTrcML, a plasmid containing the E. coli malic enzyme gene, produced a considerable amount of malic acid along with the desired product, succinic acid. To have an insight into the intracellular metabolism, metabolic control analysis was carried out. From the results of a simulation, it was predicted that supplying additional reducing power could enhance succinic acid production. More reduced carbon substrate sorbitol was thus examined for the possibility of matching the potential during succinic acid production. When NZN111 (pTrcML) was cultured in LB medium containing 20 g sorbitol/l under a CO2 atmosphere, 10 g succinic acid/l was produced. The apparent yield of succinic acid was 1.1 g succinic acid/g sorbitol, which is 85% of the maximum theoretical yield. Therefore, it was found that redox balancing was important for the enhanced production of succinic acid in metabolically engineered E. coli.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s00253-001-0899-y", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1083533", 
        "issn": [
          "0175-7598", 
          "1432-0614"
        ], 
        "name": "Applied Microbiology and Biotechnology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "3", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "58"
      }
    ], 
    "name": "Importance of redox balance on the production of succinic acid by metabolically engineered Escherichia coli", 
    "pagination": "286-290", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "b13f8fb9bf189903af7ba48a3e0cbf846b13a813a5ba56bf1258f8a20d0337d4"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "11935177"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "8406612"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00253-001-0899-y"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1037208698"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00253-001-0899-y", 
      "https://app.dimensions.ai/details/publication/pub.1037208698"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-10T15:52", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8664_00000514.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007%2Fs00253-001-0899-y"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00253-001-0899-y'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00253-001-0899-y'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00253-001-0899-y'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00253-001-0899-y'


 

This table displays all metadata directly associated to this object as RDF triples.

116 TRIPLES      20 PREDICATES      39 URIs      31 LITERALS      19 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00253-001-0899-y schema:about N0051fad9224041af8cc785f0b5c7ae9d
2 N4a17e8fac2fe44c5925e6c59a631a2ab
3 N6f0a2186de76471f9b3d919772c9222f
4 Na735610e157640188c26c53906345206
5 Nb40517b45c764f0da921511aa99f0932
6 Nc5008056391e4fd0929e6ec1a7612d96
7 Nc64c103d1ec945a58b51457acffe840d
8 Ncdeddd044e1842d0a378411b209e55a8
9 Ne1e46f1098244cf9adfc06ac6ca91b0d
10 Ne478d0b8ab3242c7ab45602324f8b160
11 anzsrc-for:06
12 anzsrc-for:0605
13 schema:author Nd65852669163416d8eb147977ceafe2b
14 schema:datePublished 2002-03
15 schema:datePublishedReg 2002-03-01
16 schema:description We had previously shown that succinic acid production in a pfl ldhA double mutant strain of Escherichia coli could be enhanced by amplifying the malic enzyme activity. However, recombinant E. coli NZN111 (F- Apfl::Cam ldhA::Kan) harboring pTrcML, a plasmid containing the E. coli malic enzyme gene, produced a considerable amount of malic acid along with the desired product, succinic acid. To have an insight into the intracellular metabolism, metabolic control analysis was carried out. From the results of a simulation, it was predicted that supplying additional reducing power could enhance succinic acid production. More reduced carbon substrate sorbitol was thus examined for the possibility of matching the potential during succinic acid production. When NZN111 (pTrcML) was cultured in LB medium containing 20 g sorbitol/l under a CO2 atmosphere, 10 g succinic acid/l was produced. The apparent yield of succinic acid was 1.1 g succinic acid/g sorbitol, which is 85% of the maximum theoretical yield. Therefore, it was found that redox balancing was important for the enhanced production of succinic acid in metabolically engineered E. coli.
17 schema:genre research_article
18 schema:inLanguage en
19 schema:isAccessibleForFree false
20 schema:isPartOf Na9f684c058114f858d57bcea8d82c220
21 Nafa4d97f26ff4430ab4112d620989db0
22 sg:journal.1083533
23 schema:name Importance of redox balance on the production of succinic acid by metabolically engineered Escherichia coli
24 schema:pagination 286-290
25 schema:productId N38f19fed997d48a6a0f8af26f120e7bc
26 N70ddfc37a16d490fb1d13e1a717a9df7
27 N9efe3903bc6c46748f31beff923ff11b
28 Nc9ee24487cfc4a12a51154b5dd6a5781
29 Nd9eba879bf3d4b559174fc47bb1ee0c4
30 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037208698
31 https://doi.org/10.1007/s00253-001-0899-y
32 schema:sdDatePublished 2019-04-10T15:52
33 schema:sdLicense https://scigraph.springernature.com/explorer/license/
34 schema:sdPublisher N13853c9160fc4dab9cc8df7094c75298
35 schema:url http://link.springer.com/10.1007%2Fs00253-001-0899-y
36 sgo:license sg:explorer/license/
37 sgo:sdDataset articles
38 rdf:type schema:ScholarlyArticle
39 N0051fad9224041af8cc785f0b5c7ae9d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
40 schema:name Succinic Acid
41 rdf:type schema:DefinedTerm
42 N00fa059ec0dc47e5b6147c150af15fd3 rdf:first sg:person.01111321475.48
43 rdf:rest rdf:nil
44 N13853c9160fc4dab9cc8df7094c75298 schema:name Springer Nature - SN SciGraph project
45 rdf:type schema:Organization
46 N38f19fed997d48a6a0f8af26f120e7bc schema:name pubmed_id
47 schema:value 11935177
48 rdf:type schema:PropertyValue
49 N4a17e8fac2fe44c5925e6c59a631a2ab schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
50 schema:name Escherichia coli
51 rdf:type schema:DefinedTerm
52 N6f0a2186de76471f9b3d919772c9222f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
53 schema:name Fermentation
54 rdf:type schema:DefinedTerm
55 N70ddfc37a16d490fb1d13e1a717a9df7 schema:name dimensions_id
56 schema:value pub.1037208698
57 rdf:type schema:PropertyValue
58 N9efe3903bc6c46748f31beff923ff11b schema:name doi
59 schema:value 10.1007/s00253-001-0899-y
60 rdf:type schema:PropertyValue
61 Na735610e157640188c26c53906345206 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
62 schema:name Malates
63 rdf:type schema:DefinedTerm
64 Na9f684c058114f858d57bcea8d82c220 schema:issueNumber 3
65 rdf:type schema:PublicationIssue
66 Nafa4d97f26ff4430ab4112d620989db0 schema:volumeNumber 58
67 rdf:type schema:PublicationVolume
68 Nb40517b45c764f0da921511aa99f0932 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
69 schema:name Sorbitol
70 rdf:type schema:DefinedTerm
71 Nc5008056391e4fd0929e6ec1a7612d96 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
72 schema:name Carbon Dioxide
73 rdf:type schema:DefinedTerm
74 Nc64c103d1ec945a58b51457acffe840d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
75 schema:name Genetic Engineering
76 rdf:type schema:DefinedTerm
77 Nc9ee24487cfc4a12a51154b5dd6a5781 schema:name nlm_unique_id
78 schema:value 8406612
79 rdf:type schema:PropertyValue
80 Ncdeddd044e1842d0a378411b209e55a8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
81 schema:name Indicators and Reagents
82 rdf:type schema:DefinedTerm
83 Nd65852669163416d8eb147977ceafe2b rdf:first sg:person.01067521611.84
84 rdf:rest N00fa059ec0dc47e5b6147c150af15fd3
85 Nd9eba879bf3d4b559174fc47bb1ee0c4 schema:name readcube_id
86 schema:value b13f8fb9bf189903af7ba48a3e0cbf846b13a813a5ba56bf1258f8a20d0337d4
87 rdf:type schema:PropertyValue
88 Ne1e46f1098244cf9adfc06ac6ca91b0d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
89 schema:name Culture Media
90 rdf:type schema:DefinedTerm
91 Ne478d0b8ab3242c7ab45602324f8b160 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
92 schema:name Oxidation-Reduction
93 rdf:type schema:DefinedTerm
94 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
95 schema:name Biological Sciences
96 rdf:type schema:DefinedTerm
97 anzsrc-for:0605 schema:inDefinedTermSet anzsrc-for:
98 schema:name Microbiology
99 rdf:type schema:DefinedTerm
100 sg:journal.1083533 schema:issn 0175-7598
101 1432-0614
102 schema:name Applied Microbiology and Biotechnology
103 rdf:type schema:Periodical
104 sg:person.01067521611.84 schema:affiliation https://www.grid.ac/institutes/grid.37172.30
105 schema:familyName Hong
106 schema:givenName S.
107 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01067521611.84
108 rdf:type schema:Person
109 sg:person.01111321475.48 schema:affiliation https://www.grid.ac/institutes/grid.37172.30
110 schema:familyName Lee
111 schema:givenName S.
112 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01111321475.48
113 rdf:type schema:Person
114 https://www.grid.ac/institutes/grid.37172.30 schema:alternateName Korea Advanced Institute of Science and Technology
115 schema:name Metabolic and Biomolecular Engineering National Research Laboratory, Department of Chemical Engineering and BioProcess Engineering Research Center, Korea Advanced Institute of Science and Technology, 373-1 Kusong-dong, Yusong-gu, Taejon 305-701, Korea
116 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...