Cloning, sequencing, and homology analysis of nonhuman primate Fas/Fas-ligand and co-stimulatory molecules View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2001-05

AUTHORS

Francois Villinger, Pavel Bostik, Ann Mayne, Christopher L. King, Claude P. Genain, Walter R. Weiss, Aftab A. Ansari

ABSTRACT

The finding that a single administration of select recombinant human cytokines to nonhuman primates leads to potent cytokine-neutralizing antibody responses in the heterologous host despite >95% homology at the nucleotide and protein level prompted our laboratory to clone, sequence, and prepare recombinant nonhuman primate cytokines, chemokines, growth factors, and other immunoregulatory molecules. In the present report, we present findings on the gene sequences encoding the nonhuman primate homologues of human CD80, CD86, their ligands CD28 and CD152, CD154, CD95, and CD95-L from rhesus macaques and for phylogenetic analysis from pig-tailed macaques, African sooty mangabey monkeys, baboons, and vervets as well as select molecules from the New World aotus and marmoset monkeys. With the exception of CD95, the homology between nonhuman primate and human co-stimulatory molecules was above 95%. In contrast, CD95 was only 89.2% homologous to human CD95, but the differences were essentially found in the transmembrane and intracellular (death) domains. The extracellular portion of CD95 was more homologous which was in accordance with approximately 98% homology between Old World monkey and human CD95-L. In general, sequences from the New World monkey species appeared equidistant to sequences from Old World species and humans in terms of homology suggesting distinct evolutionary patterns. Of interest was the isolation of various splice variants of monkey CD86, CD152 (CTLA-4), CD154, and CD95 transcripts. This is also the first report documenting the occurrence of natural CD86 variants with deleted transmembrane domains, found both in sooty mangabeys and baboon RNA samples. Monkey CD95 showed various deletions and addition of residues in the transmembrane and intracytoplasmic domains compared with human CD95 and between Old and New World species. Subcloning of rhesus CD154 into an expression vector demonstrated expression of a functional protein in cell culture. The other genes are being cloned into expression vectors for the preparation and biological characterization of the nonhuman primate molecules. These investigations will provide novel reagents for in vivo use as immunomodulatory reagents in nonhuman primates in studies which may provide a rationale for their use in humans. More... »

PAGES

315-328

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s002510100322

DOI

http://dx.doi.org/10.1007/s002510100322

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1004200509

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/11491535


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Abatacept", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antigens, CD", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antigens, Differentiation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "B7-1 Antigen", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "CTLA-4 Antigen", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cloning, Molecular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Evolution, Molecular", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fas Ligand Protein", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Haplorhini", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Immunoconjugates", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Leukocytes, Mononuclear", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Membrane Glycoproteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Analysis, DNA", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Sequence Homology, Amino Acid", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "fas Receptor", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Emory University", 
          "id": "https://www.grid.ac/institutes/grid.189967.8", 
          "name": [
            "Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA 30322, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Villinger", 
        "givenName": "Francois", 
        "id": "sg:person.01025200260.11", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01025200260.11"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Emory University", 
          "id": "https://www.grid.ac/institutes/grid.189967.8", 
          "name": [
            "Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA 30322, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bostik", 
        "givenName": "Pavel", 
        "id": "sg:person.0753367057.54", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0753367057.54"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Emory University", 
          "id": "https://www.grid.ac/institutes/grid.189967.8", 
          "name": [
            "Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA 30322, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Mayne", 
        "givenName": "Ann", 
        "id": "sg:person.01065552321.86", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01065552321.86"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Case Western Reserve University", 
          "id": "https://www.grid.ac/institutes/grid.67105.35", 
          "name": [
            "Division of Geographic Medicine, Case Western University, Cleveland, OH 44106, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "King", 
        "givenName": "Christopher L.", 
        "id": "sg:person.013211561057.09", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013211561057.09"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of California, San Francisco", 
          "id": "https://www.grid.ac/institutes/grid.266102.1", 
          "name": [
            "Department of Neurology, University of California at San Francisco, CA 94143, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Genain", 
        "givenName": "Claude P.", 
        "id": "sg:person.01051751424.20", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01051751424.20"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Naval Medical Research Center", 
          "id": "https://www.grid.ac/institutes/grid.415913.b", 
          "name": [
            "Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Weiss", 
        "givenName": "Walter R.", 
        "id": "sg:person.07567115277.04", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07567115277.04"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Emory University", 
          "id": "https://www.grid.ac/institutes/grid.189967.8", 
          "name": [
            "Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA 30322, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ansari", 
        "givenName": "Aftab A.", 
        "id": "sg:person.01316361173.51", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01316361173.51"
        ], 
        "type": "Person"
      }
    ], 
    "datePublished": "2001-05", 
    "datePublishedReg": "2001-05-01", 
    "description": "The finding that a single administration of select recombinant human cytokines to nonhuman primates leads to potent cytokine-neutralizing antibody responses in the heterologous host despite >95% homology at the nucleotide and protein level prompted our laboratory to clone, sequence, and prepare recombinant nonhuman primate cytokines, chemokines, growth factors, and other immunoregulatory molecules. In the present report, we present findings on the gene sequences encoding the nonhuman primate homologues of human CD80, CD86, their ligands CD28 and CD152, CD154, CD95, and CD95-L from rhesus macaques and for phylogenetic analysis from pig-tailed macaques, African sooty mangabey monkeys, baboons, and vervets as well as select molecules from the New World aotus and marmoset monkeys. With the exception of CD95, the homology between nonhuman primate and human co-stimulatory molecules was above 95%. In contrast, CD95 was only 89.2% homologous to human CD95, but the differences were essentially found in the transmembrane and intracellular (death) domains. The extracellular portion of CD95 was more homologous which was in accordance with approximately 98% homology between Old World monkey and human CD95-L. In general, sequences from the New World monkey species appeared equidistant to sequences from Old World species and humans in terms of homology suggesting distinct evolutionary patterns. Of interest was the isolation of various splice variants of monkey CD86, CD152 (CTLA-4), CD154, and CD95 transcripts. This is also the first report documenting the occurrence of natural CD86 variants with deleted transmembrane domains, found both in sooty mangabeys and baboon RNA samples. Monkey CD95 showed various deletions and addition of residues in the transmembrane and intracytoplasmic domains compared with human CD95 and between Old and New World species. Subcloning of rhesus CD154 into an expression vector demonstrated expression of a functional protein in cell culture. The other genes are being cloned into expression vectors for the preparation and biological characterization of the nonhuman primate molecules. These investigations will provide novel reagents for in vivo use as immunomodulatory reagents in nonhuman primates in studies which may provide a rationale for their use in humans.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s002510100322", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2451541", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2441133", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1019755", 
        "issn": [
          "0093-7711", 
          "1432-1211"
        ], 
        "name": "Immunogenetics", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "4", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "53"
      }
    ], 
    "name": "Cloning, sequencing, and homology analysis of nonhuman primate Fas/Fas-ligand and co-stimulatory molecules", 
    "pagination": "315-328", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "14042d7e3c8decc3856b88abda22381452435ecfc8da00bcd29a768c57ea7d0a"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "11491535"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0420404"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s002510100322"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1004200509"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s002510100322", 
      "https://app.dimensions.ai/details/publication/pub.1004200509"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T01:51", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8700_00000479.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/s002510100322"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s002510100322'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s002510100322'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s002510100322'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s002510100322'


 

This table displays all metadata directly associated to this object as RDF triples.

196 TRIPLES      20 PREDICATES      47 URIs      39 LITERALS      27 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s002510100322 schema:about N10374e1be0014d329c770dc8a9883dc0
2 N131069c1e346422a9a45ad18afcef3b6
3 N2957df6c4fa0482e8a0f5db1dafb7477
4 N4323afef86c34f68b35feeb4e5f18d95
5 N49c3ab03fbfb434f8e96aafa2192d462
6 N5187d01ab98d45f096852593b55b787b
7 N65fd621eed63481ca525065fd8bfb795
8 N867f51c2e1d94322a3c0483477676a18
9 N872901e5809e484f8cf94729a91a00a4
10 N9781a962a55d4bb19c2bffdf503a4729
11 Na49bd9110ddd43a082d676742d0293f8
12 Na77d658ce8a649898371c3a7d3951142
13 Nae4ad08eab4e42c7a33cc9e54bb95019
14 Nc38b5b3eb8e44e45953ae1108c875c72
15 Nc432f275209d46f8b227c5ac14488920
16 Nd46a9a675e65487cbbec003baa861655
17 Ne48b9841c27349fc886dcabb2a045c6f
18 Nfdb0cfcab2294cee9b7c9ac4ab381720
19 anzsrc-for:06
20 anzsrc-for:0604
21 schema:author N04489ca0f0bc444f9a1c6d8df7d481f3
22 schema:datePublished 2001-05
23 schema:datePublishedReg 2001-05-01
24 schema:description The finding that a single administration of select recombinant human cytokines to nonhuman primates leads to potent cytokine-neutralizing antibody responses in the heterologous host despite >95% homology at the nucleotide and protein level prompted our laboratory to clone, sequence, and prepare recombinant nonhuman primate cytokines, chemokines, growth factors, and other immunoregulatory molecules. In the present report, we present findings on the gene sequences encoding the nonhuman primate homologues of human CD80, CD86, their ligands CD28 and CD152, CD154, CD95, and CD95-L from rhesus macaques and for phylogenetic analysis from pig-tailed macaques, African sooty mangabey monkeys, baboons, and vervets as well as select molecules from the New World aotus and marmoset monkeys. With the exception of CD95, the homology between nonhuman primate and human co-stimulatory molecules was above 95%. In contrast, CD95 was only 89.2% homologous to human CD95, but the differences were essentially found in the transmembrane and intracellular (death) domains. The extracellular portion of CD95 was more homologous which was in accordance with approximately 98% homology between Old World monkey and human CD95-L. In general, sequences from the New World monkey species appeared equidistant to sequences from Old World species and humans in terms of homology suggesting distinct evolutionary patterns. Of interest was the isolation of various splice variants of monkey CD86, CD152 (CTLA-4), CD154, and CD95 transcripts. This is also the first report documenting the occurrence of natural CD86 variants with deleted transmembrane domains, found both in sooty mangabeys and baboon RNA samples. Monkey CD95 showed various deletions and addition of residues in the transmembrane and intracytoplasmic domains compared with human CD95 and between Old and New World species. Subcloning of rhesus CD154 into an expression vector demonstrated expression of a functional protein in cell culture. The other genes are being cloned into expression vectors for the preparation and biological characterization of the nonhuman primate molecules. These investigations will provide novel reagents for in vivo use as immunomodulatory reagents in nonhuman primates in studies which may provide a rationale for their use in humans.
25 schema:genre research_article
26 schema:inLanguage en
27 schema:isAccessibleForFree false
28 schema:isPartOf N8199215c5b774c55981e3bce7219dd11
29 Nd756ba0de03a4173a607210efd38ef44
30 sg:journal.1019755
31 schema:name Cloning, sequencing, and homology analysis of nonhuman primate Fas/Fas-ligand and co-stimulatory molecules
32 schema:pagination 315-328
33 schema:productId N14dc463ab9cb48698598421eb4407889
34 N7768a1fd11f34ec583036d7d1a4890bd
35 N7cdff5afeaef4e028be4f5b2df328368
36 Nb56968a0ad5b48c4b28a73aa361b4c6f
37 Nf8e65bd9351d42f5b1bb25ed1a8e3d3c
38 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004200509
39 https://doi.org/10.1007/s002510100322
40 schema:sdDatePublished 2019-04-11T01:51
41 schema:sdLicense https://scigraph.springernature.com/explorer/license/
42 schema:sdPublisher Na8646b22c45941ee9b57a125590957b7
43 schema:url http://link.springer.com/10.1007/s002510100322
44 sgo:license sg:explorer/license/
45 sgo:sdDataset articles
46 rdf:type schema:ScholarlyArticle
47 N04489ca0f0bc444f9a1c6d8df7d481f3 rdf:first sg:person.01025200260.11
48 rdf:rest Ncff7f6faa2cf429887483f070c55c964
49 N10374e1be0014d329c770dc8a9883dc0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
50 schema:name CTLA-4 Antigen
51 rdf:type schema:DefinedTerm
52 N131069c1e346422a9a45ad18afcef3b6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
53 schema:name Amino Acid Sequence
54 rdf:type schema:DefinedTerm
55 N14dc463ab9cb48698598421eb4407889 schema:name dimensions_id
56 schema:value pub.1004200509
57 rdf:type schema:PropertyValue
58 N2957df6c4fa0482e8a0f5db1dafb7477 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
59 schema:name Sequence Homology, Amino Acid
60 rdf:type schema:DefinedTerm
61 N4323afef86c34f68b35feeb4e5f18d95 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
62 schema:name Animals
63 rdf:type schema:DefinedTerm
64 N49c3ab03fbfb434f8e96aafa2192d462 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
65 schema:name Antigens, Differentiation
66 rdf:type schema:DefinedTerm
67 N4bcb3683c52046adab95df19c4caf0da rdf:first sg:person.07567115277.04
68 rdf:rest N7d2182ba651c4f8190346bfc0df1c541
69 N5187d01ab98d45f096852593b55b787b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
70 schema:name Membrane Glycoproteins
71 rdf:type schema:DefinedTerm
72 N5269ee61c4c44acf84ebc40f57d40e88 rdf:first sg:person.01051751424.20
73 rdf:rest N4bcb3683c52046adab95df19c4caf0da
74 N5f93611e9c7f402294cd7cec42d96709 rdf:first sg:person.013211561057.09
75 rdf:rest N5269ee61c4c44acf84ebc40f57d40e88
76 N65fd621eed63481ca525065fd8bfb795 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
77 schema:name Fas Ligand Protein
78 rdf:type schema:DefinedTerm
79 N7768a1fd11f34ec583036d7d1a4890bd schema:name pubmed_id
80 schema:value 11491535
81 rdf:type schema:PropertyValue
82 N7cdff5afeaef4e028be4f5b2df328368 schema:name doi
83 schema:value 10.1007/s002510100322
84 rdf:type schema:PropertyValue
85 N7d2182ba651c4f8190346bfc0df1c541 rdf:first sg:person.01316361173.51
86 rdf:rest rdf:nil
87 N8199215c5b774c55981e3bce7219dd11 schema:issueNumber 4
88 rdf:type schema:PublicationIssue
89 N867f51c2e1d94322a3c0483477676a18 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
90 schema:name fas Receptor
91 rdf:type schema:DefinedTerm
92 N872901e5809e484f8cf94729a91a00a4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
93 schema:name Haplorhini
94 rdf:type schema:DefinedTerm
95 N9781a962a55d4bb19c2bffdf503a4729 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
96 schema:name Abatacept
97 rdf:type schema:DefinedTerm
98 Na49bd9110ddd43a082d676742d0293f8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
99 schema:name Antigens, CD
100 rdf:type schema:DefinedTerm
101 Na77d658ce8a649898371c3a7d3951142 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
102 schema:name Evolution, Molecular
103 rdf:type schema:DefinedTerm
104 Na8646b22c45941ee9b57a125590957b7 schema:name Springer Nature - SN SciGraph project
105 rdf:type schema:Organization
106 Nae4ad08eab4e42c7a33cc9e54bb95019 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
107 schema:name Sequence Analysis, DNA
108 rdf:type schema:DefinedTerm
109 Nb56968a0ad5b48c4b28a73aa361b4c6f schema:name readcube_id
110 schema:value 14042d7e3c8decc3856b88abda22381452435ecfc8da00bcd29a768c57ea7d0a
111 rdf:type schema:PropertyValue
112 Nc38b5b3eb8e44e45953ae1108c875c72 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
113 schema:name Molecular Sequence Data
114 rdf:type schema:DefinedTerm
115 Nc432f275209d46f8b227c5ac14488920 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
116 schema:name Immunoconjugates
117 rdf:type schema:DefinedTerm
118 Nc7cb0540dbfb4c9da15e3837cbd3f7f6 rdf:first sg:person.01065552321.86
119 rdf:rest N5f93611e9c7f402294cd7cec42d96709
120 Ncff7f6faa2cf429887483f070c55c964 rdf:first sg:person.0753367057.54
121 rdf:rest Nc7cb0540dbfb4c9da15e3837cbd3f7f6
122 Nd46a9a675e65487cbbec003baa861655 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
123 schema:name B7-1 Antigen
124 rdf:type schema:DefinedTerm
125 Nd756ba0de03a4173a607210efd38ef44 schema:volumeNumber 53
126 rdf:type schema:PublicationVolume
127 Ne48b9841c27349fc886dcabb2a045c6f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
128 schema:name Cloning, Molecular
129 rdf:type schema:DefinedTerm
130 Nf8e65bd9351d42f5b1bb25ed1a8e3d3c schema:name nlm_unique_id
131 schema:value 0420404
132 rdf:type schema:PropertyValue
133 Nfdb0cfcab2294cee9b7c9ac4ab381720 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
134 schema:name Leukocytes, Mononuclear
135 rdf:type schema:DefinedTerm
136 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
137 schema:name Biological Sciences
138 rdf:type schema:DefinedTerm
139 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
140 schema:name Genetics
141 rdf:type schema:DefinedTerm
142 sg:grant.2441133 http://pending.schema.org/fundedItem sg:pub.10.1007/s002510100322
143 rdf:type schema:MonetaryGrant
144 sg:grant.2451541 http://pending.schema.org/fundedItem sg:pub.10.1007/s002510100322
145 rdf:type schema:MonetaryGrant
146 sg:journal.1019755 schema:issn 0093-7711
147 1432-1211
148 schema:name Immunogenetics
149 rdf:type schema:Periodical
150 sg:person.01025200260.11 schema:affiliation https://www.grid.ac/institutes/grid.189967.8
151 schema:familyName Villinger
152 schema:givenName Francois
153 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01025200260.11
154 rdf:type schema:Person
155 sg:person.01051751424.20 schema:affiliation https://www.grid.ac/institutes/grid.266102.1
156 schema:familyName Genain
157 schema:givenName Claude P.
158 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01051751424.20
159 rdf:type schema:Person
160 sg:person.01065552321.86 schema:affiliation https://www.grid.ac/institutes/grid.189967.8
161 schema:familyName Mayne
162 schema:givenName Ann
163 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01065552321.86
164 rdf:type schema:Person
165 sg:person.01316361173.51 schema:affiliation https://www.grid.ac/institutes/grid.189967.8
166 schema:familyName Ansari
167 schema:givenName Aftab A.
168 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01316361173.51
169 rdf:type schema:Person
170 sg:person.013211561057.09 schema:affiliation https://www.grid.ac/institutes/grid.67105.35
171 schema:familyName King
172 schema:givenName Christopher L.
173 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013211561057.09
174 rdf:type schema:Person
175 sg:person.0753367057.54 schema:affiliation https://www.grid.ac/institutes/grid.189967.8
176 schema:familyName Bostik
177 schema:givenName Pavel
178 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0753367057.54
179 rdf:type schema:Person
180 sg:person.07567115277.04 schema:affiliation https://www.grid.ac/institutes/grid.415913.b
181 schema:familyName Weiss
182 schema:givenName Walter R.
183 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.07567115277.04
184 rdf:type schema:Person
185 https://www.grid.ac/institutes/grid.189967.8 schema:alternateName Emory University
186 schema:name Department of Pathology and Laboratory Medicine, Emory University School of Medicine, Atlanta, GA 30322, USA
187 rdf:type schema:Organization
188 https://www.grid.ac/institutes/grid.266102.1 schema:alternateName University of California, San Francisco
189 schema:name Department of Neurology, University of California at San Francisco, CA 94143, USA
190 rdf:type schema:Organization
191 https://www.grid.ac/institutes/grid.415913.b schema:alternateName Naval Medical Research Center
192 schema:name Malaria Program, Naval Medical Research Center, Silver Spring, MD 20910, USA
193 rdf:type schema:Organization
194 https://www.grid.ac/institutes/grid.67105.35 schema:alternateName Case Western Reserve University
195 schema:name Division of Geographic Medicine, Case Western University, Cleveland, OH 44106, USA
196 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...