Effect of membrane-partitioned n-alcohols and fatty acids on pore-forming activity of a sea anemone toxin View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1997-01

AUTHORS

P. Maček, Marialucia Zecchini, Katja Stanek, Gianfranco Menestrina

ABSTRACT

Equinatoxin II, a 19.8 kDa pore-forming toxin from the sea anemone Actinia equina, was examined for hemolytic activity and permeabilization of small unilamellar lipid vesicles (SUV) in the presence of increasing amounts of n-alcohols (methanol to n-octanol) and fatty acids (palmitic and palmitoleic acid). We observed an enhancement of toxin activity which was dependent on the concentration of the membrane partitioning additive. An exception was palmitic acid which exerted a bimodal role. While at low bulk concentrations it increased toxin-induced hemolysis, above 3 µM bulk concentration it was inhibitory; in neither case was it efficient in promoting release of the fluorescent marker calcein from SUV. The increased permeabilization activity was correlated with an increase in the amount of toxin bound as indicated by changes in the intrinsic toxin fluorescence. In the case of n-alcohols, at least, these effects appeared to depend on the actual amount of alcohol present inside the membrane rather than on its specific chemical nature. This suggests that the observed effects could be due to changes of the biophysical properties of the lipid bilayer, such as thickness, lipid acyl-chain ordering, and dielectric constant induced by the partitioned additives. More... »

PAGES

155-162

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s002490050027

DOI

http://dx.doi.org/10.1007/s002490050027

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1050291911


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