Ontology type: schema:ScholarlyArticle
2001-04
AUTHORSLisa H. Pope, Martyn C. Davies, Charles A. Laughton, Clive J. Roberts, Saul J.B. Tendler, Philip M. Williams
ABSTRACTThe dynamic behaviour of DNA is of fundamental importance to many cellular processes. One principal characteristic, central to transcription and replication, is the ability of the duplex to "melt". It has recently been shown that dynamic force spectroscopy provides information about the energetics of biomolecular dissociation. We have employed this technique to investigate the unbinding of single dodecanucleotide molecules. To separate the duplex to single-stranded DNA, forces ranging from 17 to 40 pN were required over a range of loading rates. Interpretation of the dependence of melting force on loading rate revealed that the energy barrier to rupture is between 9 and 13 kcal mol-1 in height and situated 0.58 nm from an intermediate structural state. Thermal melting studies show that, prior to dissociation, the oligonucleotide underwent a transition which required between 7 and 11 kcal mol-1 in energy. Through combined dynamic force spectroscopy and thermal melting studies we show the derivation of an energy landscape to dissociate a 12-mer duplex. Until very recently, this type of information was only accessible by computational analysis. Additionally, the force spectroscopy data allow an estimation of the kinetics of duplex formation and melting. More... »
PAGES53-62
http://scigraph.springernature.com/pub.10.1007/s002490000107
DOIhttp://dx.doi.org/10.1007/s002490000107
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1014563626
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/11372533
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0306",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Physical Chemistry (incl. Structural)",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/03",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Chemical Sciences",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Base Sequence",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Calorimetry",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "DNA",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Nucleic Acid Conformation",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Nucleic Acid Denaturation",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Oligodeoxyribonucleotides",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Thermodynamics",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "University of Nottingham",
"id": "https://www.grid.ac/institutes/grid.4563.4",
"name": [
"Laboratory of Biophysics and Surface Analysis, School of Pharmaceutical Sciences, The University of Nottingham, Nottingham NG7 2RD, UK"
],
"type": "Organization"
},
"familyName": "Pope",
"givenName": "Lisa H.",
"id": "sg:person.01022452011.05",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01022452011.05"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Nottingham",
"id": "https://www.grid.ac/institutes/grid.4563.4",
"name": [
"Laboratory of Biophysics and Surface Analysis, School of Pharmaceutical Sciences, The University of Nottingham, Nottingham NG7 2RD, UK"
],
"type": "Organization"
},
"familyName": "Davies",
"givenName": "Martyn C.",
"id": "sg:person.01367077636.60",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01367077636.60"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Nottingham",
"id": "https://www.grid.ac/institutes/grid.4563.4",
"name": [
"Cancer Research Laboratories, School of Pharmaceutical Sciences, The University of Nottingham, Nottingham NG7 2RD, UK"
],
"type": "Organization"
},
"familyName": "Laughton",
"givenName": "Charles A.",
"id": "sg:person.0660242203.32",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0660242203.32"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Nottingham",
"id": "https://www.grid.ac/institutes/grid.4563.4",
"name": [
"Laboratory of Biophysics and Surface Analysis, School of Pharmaceutical Sciences, The University of Nottingham, Nottingham NG7 2RD, UK"
],
"type": "Organization"
},
"familyName": "Roberts",
"givenName": "Clive J.",
"id": "sg:person.01035336303.18",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01035336303.18"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Nottingham",
"id": "https://www.grid.ac/institutes/grid.4563.4",
"name": [
"Laboratory of Biophysics and Surface Analysis, School of Pharmaceutical Sciences, The University of Nottingham, Nottingham NG7 2RD, UK"
],
"type": "Organization"
},
"familyName": "Tendler",
"givenName": "Saul J.B.",
"id": "sg:person.015425322167.24",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015425322167.24"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Nottingham",
"id": "https://www.grid.ac/institutes/grid.4563.4",
"name": [
"Laboratory of Biophysics and Surface Analysis, School of Pharmaceutical Sciences, The University of Nottingham, Nottingham NG7 2RD, UK"
],
"type": "Organization"
},
"familyName": "Williams",
"givenName": "Philip M.",
"id": "sg:person.010727302152.58",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010727302152.58"
],
"type": "Person"
}
],
"datePublished": "2001-04",
"datePublishedReg": "2001-04-01",
"description": "The dynamic behaviour of DNA is of fundamental importance to many cellular processes. One principal characteristic, central to transcription and replication, is the ability of the duplex to \"melt\". It has recently been shown that dynamic force spectroscopy provides information about the energetics of biomolecular dissociation. We have employed this technique to investigate the unbinding of single dodecanucleotide molecules. To separate the duplex to single-stranded DNA, forces ranging from 17 to 40 pN were required over a range of loading rates. Interpretation of the dependence of melting force on loading rate revealed that the energy barrier to rupture is between 9 and 13 kcal mol-1 in height and situated 0.58 nm from an intermediate structural state. Thermal melting studies show that, prior to dissociation, the oligonucleotide underwent a transition which required between 7 and 11 kcal mol-1 in energy. Through combined dynamic force spectroscopy and thermal melting studies we show the derivation of an energy landscape to dissociate a 12-mer duplex. Until very recently, this type of information was only accessible by computational analysis. Additionally, the force spectroscopy data allow an estimation of the kinetics of duplex formation and melting.",
"genre": "research_article",
"id": "sg:pub.10.1007/s002490000107",
"inLanguage": [
"en"
],
"isAccessibleForFree": false,
"isPartOf": [
{
"id": "sg:journal.1313667",
"issn": [
"0175-7571",
"1432-1017"
],
"name": "European Biophysics Journal",
"type": "Periodical"
},
{
"issueNumber": "1",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "30"
}
],
"name": "Force-induced melting of a short DNA double helix",
"pagination": "53-62",
"productId": [
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"456166fdf0679cbc8d1eb3d351e5f47544d6d149e30337bcbd5873222dd96813"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"11372533"
]
},
{
"name": "nlm_unique_id",
"type": "PropertyValue",
"value": [
"8409413"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/s002490000107"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1014563626"
]
}
],
"sameAs": [
"https://doi.org/10.1007/s002490000107",
"https://app.dimensions.ai/details/publication/pub.1014563626"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-10T19:03",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000001_0000000264/records_8678_00000487.jsonl",
"type": "ScholarlyArticle",
"url": "http://link.springer.com/10.1007/s002490000107"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s002490000107'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s002490000107'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s002490000107'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s002490000107'
This table displays all metadata directly associated to this object as RDF triples.
133 TRIPLES
20 PREDICATES
36 URIs
28 LITERALS
16 BLANK NODES