Application of novel analytical ultracentrifuge analysis to solutions of fungal mannans View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2017-04

AUTHORS

Richard B. Gillis, Gary G. Adams, David T. M. Besong, Eva Machová, Anna Ebringerová, Arthur J. Rowe, Stephen E. Harding, Trushar R. Patel

ABSTRACT

Polysaccharides, the most abundant biopolymers, are required for a host of activities in lower organisms, animals, and plants. Their solution characterization is challenging due to their complex shape, heterogeneity, and size. Here, recently developed data analysis approaches were applied for traditional sedimentation equilibrium and velocity methods in order to investigate the molar mass distribution(s) of a subtype of polysaccharide, namely, mannans from four Candida spp. The molecular weight distributions of these mannans were studied using two recently developed equilibrium approaches: SEDFIT-MSTAR and MULTISIG, resulting in corroboratory distribution profiles. Additionally, sedimentation velocity data for all four mannans, analyzed using ls-g*(s) and Extended Fujita approaches, suggest that two of the fungal mannans (FM-1 and FM-3) have a unimodal distribution of molecular species whereas two others (FM-2 and FM-4) displayed bi-modal and broad distributions, respectively: this demonstrates considerable molecular heterogeneity in these polysaccharides, consistent with previous observations of mannans and polysaccharides in general. These methods not only have applications for the characterization of mannans but for other biopolymers such as polysaccharides, DNA, and proteins (including intrinsically disordered proteins). More... »

PAGES

235-245

References to SciGraph publications

  • 2013-10. MultiSig: a new high-precision approach to the analysis of complex biomolecular systems in EUROPEAN BIOPHYSICS JOURNAL
  • 2005. Hemicellulose in POLYSACCHARIDES I
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    http://scigraph.springernature.com/pub.10.1007/s00249-016-1159-5

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    http://dx.doi.org/10.1007/s00249-016-1159-5

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/27444285


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