Oral Microbial Species and Virulence Factors Associated with Oral Squamous Cell Carcinoma View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2020-11-06

AUTHORS

Manolito G. Torralba, Gajender Aleti, Weizhong Li, Kelvin Jens Moncera, Yi-Han Lin, Yanbao Yu, Michal M. Masternak, Wojciech Golusinski, Pawel Golusinski, Katarzyna Lamperska, Anna Edlund, Marcelo Freire, Karen E. Nelson

ABSTRACT

The human microbiome has been the focus of numerous research efforts to elucidate the pathogenesis of human diseases including cancer. Oral cancer mortality is high when compared with other cancers, as diagnosis often occurs during late stages. Its prevalence has increased in the USA over the past decade and accounts for over 40,000 new cancer patients each year. Additionally, oral cancer pathogenesis is not fully understood and is likely multifactorial. To unravel the relationships that are associated with the oral microbiome and their virulence factors, we used 16S rDNA and metagenomic sequencing to characterize the microbial composition and functional content in oral squamous cell carcinoma (OSCC) tumor tissue, non-tumor tissue, and saliva from 18 OSCC patients. Results indicate a higher number of bacteria belonging to the Fusobacteria, Bacteroidetes, and Firmicutes phyla associated with tumor tissue when compared with all other sample types. Additionally, saliva metaproteomics revealed a significant increase of Prevotella in five OSCC subjects, while Corynebacterium was mostly associated with ten healthy subjects. Lastly, we determined that there are adhesion and virulence factors associated with Streptococcus gordonii as well as from known oral pathogens belonging to the Fusobacterium genera found mostly in OSCC tissues. From these results, we propose that not only will the methods utilized in this study drastically improve OSCC diagnostics, but the organisms and specific virulence factors from the phyla detected in tumor tissue may be excellent biomarkers for characterizing disease progression. More... »

PAGES

1030-1046

References to SciGraph publications

  • 2010-04-11. QIIME allows analysis of high-throughput community sequencing data in NATURE METHODS
  • 2018-11-27. Oxidative stress in the oral cavity is driven by individual-specific bacterial communities in NPJ BIOFILMS AND MICROBIOMES
  • 2009-12-15. Defining the healthy "core microbiome" of oral microbial communities in BMC MICROBIOLOGY
  • 2012-07-20. Comparison of oral microbiota in tumor and non-tumor tissues of patients with oral squamous cell carcinoma in BMC MICROBIOLOGY
  • 2014-12-05. Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2 in GENOME BIOLOGY
  • 2017-09-18. Variations in oral microbiota associated with oral cancer in SCIENTIFIC REPORTS
  • 1993-12. Biologic markers, cellular differentiation, and metastatic head and neck cancer in EUROPEAN ARCHIVES OF OTO-RHINO-LARYNGOLOGY
  • 2011-12-28. Adherence to Streptococci Facilitates Fusobacterium nucleatum Integration into an Oral Microbial Community in MICROBIAL ECOLOGY
  • 2018-12-13. Fusobacterium nucleatum — symbiont, opportunist and oncobacterium in NATURE REVIEWS MICROBIOLOGY
  • 2018-11-12. Increased virulence of the oral microbiome in oral squamous cell carcinoma revealed by metatranscriptome analyses in INTERNATIONAL JOURNAL OF ORAL SCIENCE
  • 2015-04-27. Sequencing and beyond: integrating molecular 'omics' for microbial community profiling in NATURE REVIEWS MICROBIOLOGY
  • 1994-11-01. An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database in JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
  • 2017-02-07. Microbiomic differences in tumor and paired-normal tissue in head and neck squamous cell carcinomas in GENOME MEDICINE
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00248-020-01596-5

    DOI

    http://dx.doi.org/10.1007/s00248-020-01596-5

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1132365773

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/33155101


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/05", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Environmental Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0503", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Soil Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0602", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Ecology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0605", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Microbiology", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Carcinoma, Squamous Cell", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Head and Neck Neoplasms", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Mouth Neoplasms", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "RNA, Ribosomal, 16S", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Squamous Cell Carcinoma of Head and Neck", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Virulence Factors", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Department of Genomic Medicine, J. Craig Venter Institute, 4120 Capricorn Lane, 92037, La Jolla, CA, USA", 
              "id": "http://www.grid.ac/institutes/grid.469946.0", 
              "name": [
                "Department of Genomic Medicine, J. Craig Venter Institute, 4120 Capricorn Lane, 92037, La Jolla, CA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Torralba", 
            "givenName": "Manolito G.", 
            "id": "sg:person.01016467116.43", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01016467116.43"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Genomic Medicine, J. Craig Venter Institute, 4120 Capricorn Lane, 92037, La Jolla, CA, USA", 
              "id": "http://www.grid.ac/institutes/grid.469946.0", 
              "name": [
                "Department of Genomic Medicine, J. Craig Venter Institute, 4120 Capricorn Lane, 92037, La Jolla, CA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Aleti", 
            "givenName": "Gajender", 
            "id": "sg:person.01326137204.33", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01326137204.33"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Genomic Medicine, J. Craig Venter Institute, 4120 Capricorn Lane, 92037, La Jolla, CA, USA", 
              "id": "http://www.grid.ac/institutes/grid.469946.0", 
              "name": [
                "Department of Genomic Medicine, J. Craig Venter Institute, 4120 Capricorn Lane, 92037, La Jolla, CA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Li", 
            "givenName": "Weizhong", 
            "id": "sg:person.0754470467.52", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0754470467.52"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Genomic Medicine, J. Craig Venter Institute, 4120 Capricorn Lane, 92037, La Jolla, CA, USA", 
              "id": "http://www.grid.ac/institutes/grid.469946.0", 
              "name": [
                "Department of Genomic Medicine, J. Craig Venter Institute, 4120 Capricorn Lane, 92037, La Jolla, CA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Moncera", 
            "givenName": "Kelvin Jens", 
            "id": "sg:person.01110441327.81", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01110441327.81"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Genomic Medicine, J. Craig Venter Institute, 4120 Capricorn Lane, 92037, La Jolla, CA, USA", 
              "id": "http://www.grid.ac/institutes/grid.469946.0", 
              "name": [
                "Department of Genomic Medicine, J. Craig Venter Institute, 4120 Capricorn Lane, 92037, La Jolla, CA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Lin", 
            "givenName": "Yi-Han", 
            "id": "sg:person.0602050063.09", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0602050063.09"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Genomic Medicine, J. Craig Venter Institute, 9605 Medical Center Drive Suite 150, 20850, Rockville, MD, USA", 
              "id": "http://www.grid.ac/institutes/grid.469946.0", 
              "name": [
                "Department of Genomic Medicine, J. Craig Venter Institute, 9605 Medical Center Drive Suite 150, 20850, Rockville, MD, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Yu", 
            "givenName": "Yanbao", 
            "id": "sg:person.01314141327.80", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01314141327.80"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, 6900 Lake Nona Blvd, Central Florida Blvd, 32827, Orlando, FL, USA", 
              "id": "http://www.grid.ac/institutes/grid.170430.1", 
              "name": [
                "Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, 6900 Lake Nona Blvd, Central Florida Blvd, 32827, Orlando, FL, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Masternak", 
            "givenName": "Michal M.", 
            "id": "sg:person.01102657767.81", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01102657767.81"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Head and Neck Surgery, Poznan University of Medical Sciences, The Greater Poland Cancer Centre, Garbary 15, 61-866, Poznan, Poland", 
              "id": "http://www.grid.ac/institutes/grid.22254.33", 
              "name": [
                "Department of Head and Neck Surgery, Poznan University of Medical Sciences, The Greater Poland Cancer Centre, Garbary 15, 61-866, Poznan, Poland"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Golusinski", 
            "givenName": "Wojciech", 
            "id": "sg:person.01274735371.00", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01274735371.00"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Otolaryngology and Maxillofacial Surgery, University of Zielona Gora, Podgo\u0301rna 50, 65-246, Zielona Go\u0301ra, Poland", 
              "id": "http://www.grid.ac/institutes/grid.28048.36", 
              "name": [
                "Department of Head and Neck Surgery, Poznan University of Medical Sciences, The Greater Poland Cancer Centre, Garbary 15, 61-866, Poznan, Poland", 
                "Department of Otolaryngology and Maxillofacial Surgery, University of Zielona Gora, Podgo\u0301rna 50, 65-246, Zielona Go\u0301ra, Poland"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Golusinski", 
            "givenName": "Pawel", 
            "id": "sg:person.01231256733.66", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01231256733.66"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Laboratory of Cancer Genetics, Greater Poland Cancer Centre, 15th Garbary Street, room 5025, 61-866, Poznan, Poland", 
              "id": "http://www.grid.ac/institutes/grid.418300.e", 
              "name": [
                "Laboratory of Cancer Genetics, Greater Poland Cancer Centre, 15th Garbary Street, room 5025, 61-866, Poznan, Poland"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Lamperska", 
            "givenName": "Katarzyna", 
            "id": "sg:person.01010676621.08", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01010676621.08"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Genomic Medicine, J. Craig Venter Institute, 4120 Capricorn Lane, 92037, La Jolla, CA, USA", 
              "id": "http://www.grid.ac/institutes/grid.469946.0", 
              "name": [
                "Department of Genomic Medicine, J. Craig Venter Institute, 4120 Capricorn Lane, 92037, La Jolla, CA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Edlund", 
            "givenName": "Anna", 
            "id": "sg:person.01323423765.55", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01323423765.55"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Genomic Medicine, J. Craig Venter Institute, 4120 Capricorn Lane, 92037, La Jolla, CA, USA", 
              "id": "http://www.grid.ac/institutes/grid.469946.0", 
              "name": [
                "Department of Genomic Medicine, J. Craig Venter Institute, 4120 Capricorn Lane, 92037, La Jolla, CA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Freire", 
            "givenName": "Marcelo", 
            "id": "sg:person.0751341246.61", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0751341246.61"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Genomic Medicine, J. Craig Venter Institute, 4120 Capricorn Lane, 92037, La Jolla, CA, USA", 
              "id": "http://www.grid.ac/institutes/grid.469946.0", 
              "name": [
                "Department of Genomic Medicine, J. Craig Venter Institute, 4120 Capricorn Lane, 92037, La Jolla, CA, USA"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Nelson", 
            "givenName": "Karen E.", 
            "id": "sg:person.01213406210.22", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01213406210.22"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1038/s41522-018-0072-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1110105630", 
              "https://doi.org/10.1038/s41522-018-0072-3"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/s41579-018-0129-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1110584106", 
              "https://doi.org/10.1038/s41579-018-0129-6"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nrmicro3451", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000287747", 
              "https://doi.org/10.1038/nrmicro3451"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s13059-014-0550-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1015222646", 
              "https://doi.org/10.1186/s13059-014-0550-8"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1471-2180-9-259", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051994048", 
              "https://doi.org/10.1186/1471-2180-9-259"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/1471-2180-12-144", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053539778", 
              "https://doi.org/10.1186/1471-2180-12-144"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00248-011-9989-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052133554", 
              "https://doi.org/10.1007/s00248-011-9989-2"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/s41368-018-0037-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1109823467", 
              "https://doi.org/10.1038/s41368-018-0037-7"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00181082", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010440711", 
              "https://doi.org/10.1007/bf00181082"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/s41598-017-11779-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1091675719", 
              "https://doi.org/10.1038/s41598-017-11779-9"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1016/1044-0305(94)80016-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1018629105", 
              "https://doi.org/10.1016/1044-0305(94)80016-2"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1186/s13073-017-0405-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1083720076", 
              "https://doi.org/10.1186/s13073-017-0405-5"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/nmeth.f.303", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009032055", 
              "https://doi.org/10.1038/nmeth.f.303"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2020-11-06", 
        "datePublishedReg": "2020-11-06", 
        "description": "The human microbiome has been the focus of numerous research efforts to elucidate the pathogenesis of human diseases including cancer. Oral cancer mortality is high when compared with other cancers, as diagnosis often occurs during late stages. Its prevalence has increased in the USA over the past decade and accounts for over 40,000 new cancer patients each year. Additionally, oral cancer pathogenesis\u00a0is not fully understood and is likely multifactorial. To unravel the relationships that are associated with the oral microbiome and their virulence factors, we used 16S rDNA and metagenomic sequencing to characterize the microbial composition and functional content in oral squamous cell carcinoma (OSCC) tumor tissue, non-tumor tissue, and saliva from 18 OSCC patients. Results indicate a higher number of bacteria belonging to the Fusobacteria, Bacteroidetes, and Firmicutes phyla associated with tumor tissue when compared with all other sample types. Additionally, saliva metaproteomics revealed a significant increase of Prevotella in five OSCC subjects, while Corynebacterium was mostly associated with ten healthy subjects. Lastly, we determined that there are adhesion and virulence factors associated with Streptococcus gordonii as well as from known oral pathogens belonging to the Fusobacterium genera found mostly in OSCC tissues. From these results, we propose that not only will the methods utilized in this study drastically improve OSCC diagnostics, but the organisms and specific virulence factors from the phyla detected in tumor tissue may be excellent biomarkers for characterizing disease progression.", 
        "genre": "article", 
        "id": "sg:pub.10.1007/s00248-020-01596-5", 
        "isAccessibleForFree": true, 
        "isFundedItemOf": [
          {
            "id": "sg:grant.4241920", 
            "type": "MonetaryGrant"
          }
        ], 
        "isPartOf": [
          {
            "id": "sg:journal.1081458", 
            "issn": [
              "0095-3628", 
              "1432-184X"
            ], 
            "name": "Microbial Ecology", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "4", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "82"
          }
        ], 
        "keywords": [
          "tumor tissue", 
          "virulence factors", 
          "oral squamous cell carcinoma", 
          "oral cancer mortality", 
          "squamous cell carcinoma", 
          "oral cancer pathogenesis", 
          "new cancer patients", 
          "non-tumor tissues", 
          "specific virulence factors", 
          "OSCC subjects", 
          "OSCC patients", 
          "cancer mortality", 
          "cell carcinoma", 
          "cancer patients", 
          "OSCC tissues", 
          "disease progression", 
          "healthy subjects", 
          "oral microbiome", 
          "cancer pathogenesis", 
          "oral pathogens", 
          "oral microbial species", 
          "Fusobacterium genus", 
          "excellent biomarker", 
          "patients", 
          "significant increase", 
          "pathogenesis", 
          "cancer", 
          "tissue", 
          "human microbiome", 
          "human diseases", 
          "Streptococcus gordonii", 
          "microbial composition", 
          "Firmicutes phylum", 
          "subjects", 
          "microbiome", 
          "metagenomic sequencing", 
          "higher number", 
          "factors", 
          "carcinoma", 
          "later stages", 
          "mortality", 
          "prevalence", 
          "disease", 
          "diagnosis", 
          "progression", 
          "biomarkers", 
          "Prevotella", 
          "saliva", 
          "sample types", 
          "Fusobacteria", 
          "past decade", 
          "pathogens", 
          "years", 
          "gordonii", 
          "sequencing", 
          "Corynebacterium", 
          "study", 
          "increase", 
          "microbial species", 
          "research efforts", 
          "results", 
          "bacteria", 
          "diagnostics", 
          "stage", 
          "adhesion", 
          "Bacteroidetes", 
          "relationship", 
          "number", 
          "types", 
          "decades", 
          "organisms", 
          "focus", 
          "USA", 
          "efforts", 
          "functional content", 
          "method", 
          "phyla", 
          "content", 
          "numerous research efforts", 
          "metaproteomics", 
          "species", 
          "composition", 
          "genus", 
          "rDNA"
        ], 
        "name": "Oral Microbial Species and Virulence Factors Associated with Oral Squamous Cell Carcinoma", 
        "pagination": "1030-1046", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1132365773"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/s00248-020-01596-5"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "33155101"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/s00248-020-01596-5", 
          "https://app.dimensions.ai/details/publication/pub.1132365773"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2022-12-01T06:40", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20221201/entities/gbq_results/article/article_830.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1007/s00248-020-01596-5"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00248-020-01596-5'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00248-020-01596-5'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00248-020-01596-5'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00248-020-01596-5'


     

    This table displays all metadata directly associated to this object as RDF triples.

    338 TRIPLES      21 PREDICATES      132 URIs      108 LITERALS      14 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/s00248-020-01596-5 schema:about N4cef569949664bf4ba459ce15562f1e7
    2 N5ba8ae74758843859bcbbd7fc94c8315
    3 N66a750ccac384599be38354cae96ee47
    4 Nb58119177fce4d2fa3e12ea35e2853c9
    5 Nd7aa0575b3b8468da3deadc2666dd379
    6 Ne5daf18dc76f41d4bcabe6df17433208
    7 Ne5feb43b808c4d589d5c45eb0fb5fc47
    8 anzsrc-for:05
    9 anzsrc-for:0503
    10 anzsrc-for:06
    11 anzsrc-for:0602
    12 anzsrc-for:0605
    13 schema:author Ne211f009c7da4ab88c096071029d9143
    14 schema:citation sg:pub.10.1007/bf00181082
    15 sg:pub.10.1007/s00248-011-9989-2
    16 sg:pub.10.1016/1044-0305(94)80016-2
    17 sg:pub.10.1038/nmeth.f.303
    18 sg:pub.10.1038/nrmicro3451
    19 sg:pub.10.1038/s41368-018-0037-7
    20 sg:pub.10.1038/s41522-018-0072-3
    21 sg:pub.10.1038/s41579-018-0129-6
    22 sg:pub.10.1038/s41598-017-11779-9
    23 sg:pub.10.1186/1471-2180-12-144
    24 sg:pub.10.1186/1471-2180-9-259
    25 sg:pub.10.1186/s13059-014-0550-8
    26 sg:pub.10.1186/s13073-017-0405-5
    27 schema:datePublished 2020-11-06
    28 schema:datePublishedReg 2020-11-06
    29 schema:description The human microbiome has been the focus of numerous research efforts to elucidate the pathogenesis of human diseases including cancer. Oral cancer mortality is high when compared with other cancers, as diagnosis often occurs during late stages. Its prevalence has increased in the USA over the past decade and accounts for over 40,000 new cancer patients each year. Additionally, oral cancer pathogenesis is not fully understood and is likely multifactorial. To unravel the relationships that are associated with the oral microbiome and their virulence factors, we used 16S rDNA and metagenomic sequencing to characterize the microbial composition and functional content in oral squamous cell carcinoma (OSCC) tumor tissue, non-tumor tissue, and saliva from 18 OSCC patients. Results indicate a higher number of bacteria belonging to the Fusobacteria, Bacteroidetes, and Firmicutes phyla associated with tumor tissue when compared with all other sample types. Additionally, saliva metaproteomics revealed a significant increase of Prevotella in five OSCC subjects, while Corynebacterium was mostly associated with ten healthy subjects. Lastly, we determined that there are adhesion and virulence factors associated with Streptococcus gordonii as well as from known oral pathogens belonging to the Fusobacterium genera found mostly in OSCC tissues. From these results, we propose that not only will the methods utilized in this study drastically improve OSCC diagnostics, but the organisms and specific virulence factors from the phyla detected in tumor tissue may be excellent biomarkers for characterizing disease progression.
    30 schema:genre article
    31 schema:isAccessibleForFree true
    32 schema:isPartOf Nb53ffa1a3a01466db4285301c446ef14
    33 Ncf78183123874a8ba80151e54c336620
    34 sg:journal.1081458
    35 schema:keywords Bacteroidetes
    36 Corynebacterium
    37 Firmicutes phylum
    38 Fusobacteria
    39 Fusobacterium genus
    40 OSCC patients
    41 OSCC subjects
    42 OSCC tissues
    43 Prevotella
    44 Streptococcus gordonii
    45 USA
    46 adhesion
    47 bacteria
    48 biomarkers
    49 cancer
    50 cancer mortality
    51 cancer pathogenesis
    52 cancer patients
    53 carcinoma
    54 cell carcinoma
    55 composition
    56 content
    57 decades
    58 diagnosis
    59 diagnostics
    60 disease
    61 disease progression
    62 efforts
    63 excellent biomarker
    64 factors
    65 focus
    66 functional content
    67 genus
    68 gordonii
    69 healthy subjects
    70 higher number
    71 human diseases
    72 human microbiome
    73 increase
    74 later stages
    75 metagenomic sequencing
    76 metaproteomics
    77 method
    78 microbial composition
    79 microbial species
    80 microbiome
    81 mortality
    82 new cancer patients
    83 non-tumor tissues
    84 number
    85 numerous research efforts
    86 oral cancer mortality
    87 oral cancer pathogenesis
    88 oral microbial species
    89 oral microbiome
    90 oral pathogens
    91 oral squamous cell carcinoma
    92 organisms
    93 past decade
    94 pathogenesis
    95 pathogens
    96 patients
    97 phyla
    98 prevalence
    99 progression
    100 rDNA
    101 relationship
    102 research efforts
    103 results
    104 saliva
    105 sample types
    106 sequencing
    107 significant increase
    108 species
    109 specific virulence factors
    110 squamous cell carcinoma
    111 stage
    112 study
    113 subjects
    114 tissue
    115 tumor tissue
    116 types
    117 virulence factors
    118 years
    119 schema:name Oral Microbial Species and Virulence Factors Associated with Oral Squamous Cell Carcinoma
    120 schema:pagination 1030-1046
    121 schema:productId N0e537ba26ab7449fb0b2664f0e8c9255
    122 N335e8b3557d841dca3dc683fa61d96e7
    123 N5bf5cd33220c47d08ed107986a6c0ff1
    124 schema:sameAs https://app.dimensions.ai/details/publication/pub.1132365773
    125 https://doi.org/10.1007/s00248-020-01596-5
    126 schema:sdDatePublished 2022-12-01T06:40
    127 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    128 schema:sdPublisher Nbf4b9940ed1544c18f409c9af18b8b76
    129 schema:url https://doi.org/10.1007/s00248-020-01596-5
    130 sgo:license sg:explorer/license/
    131 sgo:sdDataset articles
    132 rdf:type schema:ScholarlyArticle
    133 N0e537ba26ab7449fb0b2664f0e8c9255 schema:name doi
    134 schema:value 10.1007/s00248-020-01596-5
    135 rdf:type schema:PropertyValue
    136 N240ce83da14a400ea8228d7e991f142e rdf:first sg:person.01110441327.81
    137 rdf:rest N537d4b38ff334520be61669dee67015e
    138 N335e8b3557d841dca3dc683fa61d96e7 schema:name dimensions_id
    139 schema:value pub.1132365773
    140 rdf:type schema:PropertyValue
    141 N40791183472748b2a648277c6da72bca rdf:first sg:person.01010676621.08
    142 rdf:rest N54ac6af8da824252b0adf6ee60bb6426
    143 N4cef569949664bf4ba459ce15562f1e7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    144 schema:name RNA, Ribosomal, 16S
    145 rdf:type schema:DefinedTerm
    146 N537d4b38ff334520be61669dee67015e rdf:first sg:person.0602050063.09
    147 rdf:rest Na734043c0453417da286fd30f9c64461
    148 N54ac6af8da824252b0adf6ee60bb6426 rdf:first sg:person.01323423765.55
    149 rdf:rest N96e8b426ad8f414c9b67ab29022122fe
    150 N5ba8ae74758843859bcbbd7fc94c8315 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    151 schema:name Virulence Factors
    152 rdf:type schema:DefinedTerm
    153 N5bf5cd33220c47d08ed107986a6c0ff1 schema:name pubmed_id
    154 schema:value 33155101
    155 rdf:type schema:PropertyValue
    156 N60b09a49cee64c8e8efd881c2e323efb rdf:first sg:person.01274735371.00
    157 rdf:rest Na1d37a9bb6c24a1d993f38a92b3d2fd9
    158 N66a750ccac384599be38354cae96ee47 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    159 schema:name Humans
    160 rdf:type schema:DefinedTerm
    161 N75f3ee1637354fbcb7635f8452e45048 rdf:first sg:person.01326137204.33
    162 rdf:rest N972385b336bb40f594f6f15d78c03767
    163 N96a2b5dfe98940b59567a5cf477a32bc rdf:first sg:person.01102657767.81
    164 rdf:rest N60b09a49cee64c8e8efd881c2e323efb
    165 N96e8b426ad8f414c9b67ab29022122fe rdf:first sg:person.0751341246.61
    166 rdf:rest Na4bd24c63563473abf7752ae39709374
    167 N972385b336bb40f594f6f15d78c03767 rdf:first sg:person.0754470467.52
    168 rdf:rest N240ce83da14a400ea8228d7e991f142e
    169 Na1d37a9bb6c24a1d993f38a92b3d2fd9 rdf:first sg:person.01231256733.66
    170 rdf:rest N40791183472748b2a648277c6da72bca
    171 Na4bd24c63563473abf7752ae39709374 rdf:first sg:person.01213406210.22
    172 rdf:rest rdf:nil
    173 Na734043c0453417da286fd30f9c64461 rdf:first sg:person.01314141327.80
    174 rdf:rest N96a2b5dfe98940b59567a5cf477a32bc
    175 Nb53ffa1a3a01466db4285301c446ef14 schema:issueNumber 4
    176 rdf:type schema:PublicationIssue
    177 Nb58119177fce4d2fa3e12ea35e2853c9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    178 schema:name Mouth Neoplasms
    179 rdf:type schema:DefinedTerm
    180 Nbf4b9940ed1544c18f409c9af18b8b76 schema:name Springer Nature - SN SciGraph project
    181 rdf:type schema:Organization
    182 Ncf78183123874a8ba80151e54c336620 schema:volumeNumber 82
    183 rdf:type schema:PublicationVolume
    184 Nd7aa0575b3b8468da3deadc2666dd379 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    185 schema:name Squamous Cell Carcinoma of Head and Neck
    186 rdf:type schema:DefinedTerm
    187 Ne211f009c7da4ab88c096071029d9143 rdf:first sg:person.01016467116.43
    188 rdf:rest N75f3ee1637354fbcb7635f8452e45048
    189 Ne5daf18dc76f41d4bcabe6df17433208 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    190 schema:name Carcinoma, Squamous Cell
    191 rdf:type schema:DefinedTerm
    192 Ne5feb43b808c4d589d5c45eb0fb5fc47 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    193 schema:name Head and Neck Neoplasms
    194 rdf:type schema:DefinedTerm
    195 anzsrc-for:05 schema:inDefinedTermSet anzsrc-for:
    196 schema:name Environmental Sciences
    197 rdf:type schema:DefinedTerm
    198 anzsrc-for:0503 schema:inDefinedTermSet anzsrc-for:
    199 schema:name Soil Sciences
    200 rdf:type schema:DefinedTerm
    201 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    202 schema:name Biological Sciences
    203 rdf:type schema:DefinedTerm
    204 anzsrc-for:0602 schema:inDefinedTermSet anzsrc-for:
    205 schema:name Ecology
    206 rdf:type schema:DefinedTerm
    207 anzsrc-for:0605 schema:inDefinedTermSet anzsrc-for:
    208 schema:name Microbiology
    209 rdf:type schema:DefinedTerm
    210 sg:grant.4241920 http://pending.schema.org/fundedItem sg:pub.10.1007/s00248-020-01596-5
    211 rdf:type schema:MonetaryGrant
    212 sg:journal.1081458 schema:issn 0095-3628
    213 1432-184X
    214 schema:name Microbial Ecology
    215 schema:publisher Springer Nature
    216 rdf:type schema:Periodical
    217 sg:person.01010676621.08 schema:affiliation grid-institutes:grid.418300.e
    218 schema:familyName Lamperska
    219 schema:givenName Katarzyna
    220 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01010676621.08
    221 rdf:type schema:Person
    222 sg:person.01016467116.43 schema:affiliation grid-institutes:grid.469946.0
    223 schema:familyName Torralba
    224 schema:givenName Manolito G.
    225 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01016467116.43
    226 rdf:type schema:Person
    227 sg:person.01102657767.81 schema:affiliation grid-institutes:grid.170430.1
    228 schema:familyName Masternak
    229 schema:givenName Michal M.
    230 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01102657767.81
    231 rdf:type schema:Person
    232 sg:person.01110441327.81 schema:affiliation grid-institutes:grid.469946.0
    233 schema:familyName Moncera
    234 schema:givenName Kelvin Jens
    235 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01110441327.81
    236 rdf:type schema:Person
    237 sg:person.01213406210.22 schema:affiliation grid-institutes:grid.469946.0
    238 schema:familyName Nelson
    239 schema:givenName Karen E.
    240 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01213406210.22
    241 rdf:type schema:Person
    242 sg:person.01231256733.66 schema:affiliation grid-institutes:grid.28048.36
    243 schema:familyName Golusinski
    244 schema:givenName Pawel
    245 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01231256733.66
    246 rdf:type schema:Person
    247 sg:person.01274735371.00 schema:affiliation grid-institutes:grid.22254.33
    248 schema:familyName Golusinski
    249 schema:givenName Wojciech
    250 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01274735371.00
    251 rdf:type schema:Person
    252 sg:person.01314141327.80 schema:affiliation grid-institutes:grid.469946.0
    253 schema:familyName Yu
    254 schema:givenName Yanbao
    255 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01314141327.80
    256 rdf:type schema:Person
    257 sg:person.01323423765.55 schema:affiliation grid-institutes:grid.469946.0
    258 schema:familyName Edlund
    259 schema:givenName Anna
    260 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01323423765.55
    261 rdf:type schema:Person
    262 sg:person.01326137204.33 schema:affiliation grid-institutes:grid.469946.0
    263 schema:familyName Aleti
    264 schema:givenName Gajender
    265 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01326137204.33
    266 rdf:type schema:Person
    267 sg:person.0602050063.09 schema:affiliation grid-institutes:grid.469946.0
    268 schema:familyName Lin
    269 schema:givenName Yi-Han
    270 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0602050063.09
    271 rdf:type schema:Person
    272 sg:person.0751341246.61 schema:affiliation grid-institutes:grid.469946.0
    273 schema:familyName Freire
    274 schema:givenName Marcelo
    275 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0751341246.61
    276 rdf:type schema:Person
    277 sg:person.0754470467.52 schema:affiliation grid-institutes:grid.469946.0
    278 schema:familyName Li
    279 schema:givenName Weizhong
    280 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0754470467.52
    281 rdf:type schema:Person
    282 sg:pub.10.1007/bf00181082 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010440711
    283 https://doi.org/10.1007/bf00181082
    284 rdf:type schema:CreativeWork
    285 sg:pub.10.1007/s00248-011-9989-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052133554
    286 https://doi.org/10.1007/s00248-011-9989-2
    287 rdf:type schema:CreativeWork
    288 sg:pub.10.1016/1044-0305(94)80016-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018629105
    289 https://doi.org/10.1016/1044-0305(94)80016-2
    290 rdf:type schema:CreativeWork
    291 sg:pub.10.1038/nmeth.f.303 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009032055
    292 https://doi.org/10.1038/nmeth.f.303
    293 rdf:type schema:CreativeWork
    294 sg:pub.10.1038/nrmicro3451 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000287747
    295 https://doi.org/10.1038/nrmicro3451
    296 rdf:type schema:CreativeWork
    297 sg:pub.10.1038/s41368-018-0037-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1109823467
    298 https://doi.org/10.1038/s41368-018-0037-7
    299 rdf:type schema:CreativeWork
    300 sg:pub.10.1038/s41522-018-0072-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1110105630
    301 https://doi.org/10.1038/s41522-018-0072-3
    302 rdf:type schema:CreativeWork
    303 sg:pub.10.1038/s41579-018-0129-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1110584106
    304 https://doi.org/10.1038/s41579-018-0129-6
    305 rdf:type schema:CreativeWork
    306 sg:pub.10.1038/s41598-017-11779-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1091675719
    307 https://doi.org/10.1038/s41598-017-11779-9
    308 rdf:type schema:CreativeWork
    309 sg:pub.10.1186/1471-2180-12-144 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053539778
    310 https://doi.org/10.1186/1471-2180-12-144
    311 rdf:type schema:CreativeWork
    312 sg:pub.10.1186/1471-2180-9-259 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051994048
    313 https://doi.org/10.1186/1471-2180-9-259
    314 rdf:type schema:CreativeWork
    315 sg:pub.10.1186/s13059-014-0550-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015222646
    316 https://doi.org/10.1186/s13059-014-0550-8
    317 rdf:type schema:CreativeWork
    318 sg:pub.10.1186/s13073-017-0405-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083720076
    319 https://doi.org/10.1186/s13073-017-0405-5
    320 rdf:type schema:CreativeWork
    321 grid-institutes:grid.170430.1 schema:alternateName Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, 6900 Lake Nona Blvd, Central Florida Blvd, 32827, Orlando, FL, USA
    322 schema:name Burnett School of Biomedical Sciences, College of Medicine, University of Central Florida, 6900 Lake Nona Blvd, Central Florida Blvd, 32827, Orlando, FL, USA
    323 rdf:type schema:Organization
    324 grid-institutes:grid.22254.33 schema:alternateName Department of Head and Neck Surgery, Poznan University of Medical Sciences, The Greater Poland Cancer Centre, Garbary 15, 61-866, Poznan, Poland
    325 schema:name Department of Head and Neck Surgery, Poznan University of Medical Sciences, The Greater Poland Cancer Centre, Garbary 15, 61-866, Poznan, Poland
    326 rdf:type schema:Organization
    327 grid-institutes:grid.28048.36 schema:alternateName Department of Otolaryngology and Maxillofacial Surgery, University of Zielona Gora, Podgórna 50, 65-246, Zielona Góra, Poland
    328 schema:name Department of Head and Neck Surgery, Poznan University of Medical Sciences, The Greater Poland Cancer Centre, Garbary 15, 61-866, Poznan, Poland
    329 Department of Otolaryngology and Maxillofacial Surgery, University of Zielona Gora, Podgórna 50, 65-246, Zielona Góra, Poland
    330 rdf:type schema:Organization
    331 grid-institutes:grid.418300.e schema:alternateName Laboratory of Cancer Genetics, Greater Poland Cancer Centre, 15th Garbary Street, room 5025, 61-866, Poznan, Poland
    332 schema:name Laboratory of Cancer Genetics, Greater Poland Cancer Centre, 15th Garbary Street, room 5025, 61-866, Poznan, Poland
    333 rdf:type schema:Organization
    334 grid-institutes:grid.469946.0 schema:alternateName Department of Genomic Medicine, J. Craig Venter Institute, 4120 Capricorn Lane, 92037, La Jolla, CA, USA
    335 Department of Genomic Medicine, J. Craig Venter Institute, 9605 Medical Center Drive Suite 150, 20850, Rockville, MD, USA
    336 schema:name Department of Genomic Medicine, J. Craig Venter Institute, 4120 Capricorn Lane, 92037, La Jolla, CA, USA
    337 Department of Genomic Medicine, J. Craig Venter Institute, 9605 Medical Center Drive Suite 150, 20850, Rockville, MD, USA
    338 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...