Community Analysis of Plant Biomass-Degrading Microorganisms from Obsidian Pool, Yellowstone National Park View Full Text


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Article Info

DATE

2014-10-16

AUTHORS

Tatiana A. Vishnivetskaya, Scott D. Hamilton-Brehm, Mircea Podar, Jennifer J. Mosher, Anthony V. Palumbo, Tommy J. Phelps, Martin Keller, James G. Elkins

ABSTRACT

The conversion of lignocellulosic biomass into biofuels can potentially be improved by employing robust microorganisms and enzymes that efficiently deconstruct plant polysaccharides at elevated temperatures. Many of the geothermal features of Yellowstone National Park (YNP) are surrounded by vegetation providing a source of allochthonic material to support heterotrophic microbial communities adapted to utilize plant biomass as a primary carbon and energy source. In this study, a well-known hot spring environment, Obsidian Pool (OBP), was examined for potential biomass-active microorganisms using cultivation-independent and enrichment techniques. Analysis of 33,684 archaeal and 43,784 bacterial quality-filtered 16S rRNA gene pyrosequences revealed that archaeal diversity in the main pool was higher than bacterial; however, in the vegetated area, overall bacterial diversity was significantly higher. Of notable interest was a flooded depression adjacent to OBP supporting a stand of Juncus tweedyi, a heat-tolerant rush commonly found growing near geothermal features in YNP. The microbial community from heated sediments surrounding the plants was enriched in members of the Firmicutes including potentially (hemi)cellulolytic bacteria from the genera Clostridium, Anaerobacter, Caloramator, Caldicellulosiruptor, and Thermoanaerobacter. Enrichment cultures containing model and real biomass substrates were established at a wide range of temperatures (55–85 °C). Microbial activity was observed up to 80 °C on all substrates including Avicel, xylan, switchgrass, and Populus sp. Independent of substrate, Caloramator was enriched at lower (<65 °C) temperatures while highly active cellulolytic bacteria Caldicellulosiruptor were dominant at high (>65 °C) temperatures. More... »

PAGES

333-345

References to SciGraph publications

  • 2013-04-22. Insights into archaeal evolution and symbiosis from the genomes of a nanoarchaeon and its inferred crenarchaeal host from Obsidian Pool, Yellowstone National Park in BIOLOGY DIRECT
  • 2012-12-13. Sediment microbial communities in Great Boiling Spring are controlled by temperature and distinct from water communities in THE ISME JOURNAL: MULTIDISCIPLINARY JOURNAL OF MICROBIAL ECOLOGY
  • 2011-11-06. Metagenomic analysis of a permafrost microbial community reveals a rapid response to thaw in NATURE
  • 2013-06-03. Metabolic engineering of Caldicellulosiruptor bescii yields increased hydrogen production from lignocellulosic biomass in BIOTECHNOLOGY FOR BIOFUELS
  • 2006-08-07. UniFrac – An online tool for comparing microbial community diversity in a phylogenetic context in BMC BIOINFORMATICS
  • 1991-12. Isolation and characterization of a strictly xylan-degrading Dictyoglomus from a man-made, thermophilic anaerobic environment in ARCHIVES OF MICROBIOLOGY
  • 2007-01-15. Prediction of effective genome size in metagenomic samples in GENOME BIOLOGY
  • 2010-06-16. Metabolic engineering to improve ethanol production in Thermoanaerobacter mathranii in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2008-09-19. The metagenomics RAST server – a public resource for the automatic phylogenetic and functional analysis of metagenomes in BMC BIOINFORMATICS
  • 2010-08-20. Characterization of Archaeal Community in Contaminated and Uncontaminated Surface Stream Sediments in MICROBIAL ECOLOGY
  • 2013-01-24. Thermodesulfobacterium geofontis sp. nov., a hyperthermophilic, sulfate-reducing bacterium isolated from Obsidian Pool, Yellowstone National Park in EXTREMOPHILES
  • 1992-10. Thermophilic and alkalophilic xylanases from several Dictyoglomus isolates in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2007-11. Metagenomic and functional analysis of hindgut microbiota of a wood-feeding higher termite in NATURE
  • 2006. Cellulose-decomposing Bacteria and Their Enzyme Systems in THE PROKARYOTES
  • 2013-02-28. Single-step ethanol production from lignocellulose using novel extremely thermophilic bacteria in BIOTECHNOLOGY FOR BIOFUELS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00248-014-0500-8

    DOI

    http://dx.doi.org/10.1007/s00248-014-0500-8

    DIMENSIONS

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    40 schema:description The conversion of lignocellulosic biomass into biofuels can potentially be improved by employing robust microorganisms and enzymes that efficiently deconstruct plant polysaccharides at elevated temperatures. Many of the geothermal features of Yellowstone National Park (YNP) are surrounded by vegetation providing a source of allochthonic material to support heterotrophic microbial communities adapted to utilize plant biomass as a primary carbon and energy source. In this study, a well-known hot spring environment, Obsidian Pool (OBP), was examined for potential biomass-active microorganisms using cultivation-independent and enrichment techniques. Analysis of 33,684 archaeal and 43,784 bacterial quality-filtered 16S rRNA gene pyrosequences revealed that archaeal diversity in the main pool was higher than bacterial; however, in the vegetated area, overall bacterial diversity was significantly higher. Of notable interest was a flooded depression adjacent to OBP supporting a stand of Juncus tweedyi, a heat-tolerant rush commonly found growing near geothermal features in YNP. The microbial community from heated sediments surrounding the plants was enriched in members of the Firmicutes including potentially (hemi)cellulolytic bacteria from the genera Clostridium, Anaerobacter, Caloramator, Caldicellulosiruptor, and Thermoanaerobacter. Enrichment cultures containing model and real biomass substrates were established at a wide range of temperatures (55–85 °C). Microbial activity was observed up to 80 °C on all substrates including Avicel, xylan, switchgrass, and Populus sp. Independent of substrate, Caloramator was enriched at lower (<65 °C) temperatures while highly active cellulolytic bacteria Caldicellulosiruptor were dominant at high (>65 °C) temperatures.
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