Functional Consequences of Sulfhydryl Modification of the γ-Aminobutyric Acid Transporter 1 at a Single Solvent-Exposed Cysteine Residue View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2012-12

AUTHORS

Jaison J. Omoto, Matthew J. Maestas, Ali Rahnama-Vaghef, Ye E. Choi, Gerardo Salto, Rachel V. Sanchez, Cynthia M. Anderson, Sepehr Eskandari

ABSTRACT

The aims of this study were to optimize the experimental conditions for labeling extracellularly oriented, solvent-exposed cysteine residues of γ-aminobutyric acid transporter 1 (GAT1) with the membrane-impermeant sulfhydryl reagent [2-(trimethylammonium)ethyl]methanethiosulfonate (MTSET) and to characterize the functional and pharmacological consequences of labeling on transporter steady-state and presteady-state kinetic properties. We expressed human GAT1 in Xenopus laevis oocytes and used radiotracer and electrophysiological methods to assay transporter function before and after sulfhydryl modification with MTSET. In the presence of NaCl, transporter exposure to MTSET (1-2.5 mM for 5-20 min) led to partial inhibition of GAT1-mediated transport, and this loss of function was completely reversed by the reducing reagent dithiothreitol. MTSET treatment had no functional effect on the mutant GAT1 C74A, whereas the membrane-permeant reagents N-ethylmaleimide and tetramethylrhodamine-6-maleimide inhibited GABA transport mediated by GAT1 C74A. Ion replacement experiments indicated that MTSET labeling of GAT1 could be driven to completion when valproate replaced chloride in the labeling buffer, suggesting that valproate induces a GAT1 conformation that significantly increases C74 accessibility to the extracellular fluid. Following partial inhibition by MTSET, there was a proportional reduction in both the presteady-state and steady-state macroscopic signals, and the functional and pharmacological properties of the remaining signals were indistinguishable from those of unlabeled GAT1. Therefore, covalent modification of GAT1 at C74 results in completely nonfunctional as well as electrically silent transporters. More... »

PAGES

841-857

References to SciGraph publications

  • 2004-02. Synaptic uptake and beyond: the sodium- and chloride-dependent neurotransmitter transporter family SLC6 in PFLÜGERS ARCHIV - EUROPEAN JOURNAL OF PHYSIOLOGY
  • 2002-11. Presteady-State and Steady-State Kinetics and Turnover Rate of the Mouse g-Aminobutyric Acid Transporter (mGAT3) in THE JOURNAL OF MEMBRANE BIOLOGY
  • 2005-09. Crystal structure of a bacterial homologue of Na+/Cl--dependent neurotransmitter transporters in NATURE
  • 1991-11. Cloning and expression of a functional serotonin transporter from rat brain in NATURE
  • 2005-01. Novel Properties of a Mouse γ-Aminobutyric Acid Transporter (GAT4) in THE JOURNAL OF MEMBRANE BIOLOGY
  • 2007-12. Assessing the ability of sequence-based methods to provide functional insight within membrane integral proteins: a case study analyzing the neurotransmitter/Na+ symporter family in BMC BIOINFORMATICS
  • 1995-11. A method for determining the unitary functional capacity of cloned channels and transporters expressed in Xenopus laevis oocytes in THE JOURNAL OF MEMBRANE BIOLOGY
  • 2007-12. Turnover Rate of the γ-Aminobutyric Acid Transporter GAT1 in THE JOURNAL OF MEMBRANE BIOLOGY
  • 1997-01. Presteady-State Currents of the Rabbit Na+/Glucose Cotransporter (SGLT1) in THE JOURNAL OF MEMBRANE BIOLOGY
  • 2006-01. Role of the conserved glutamine 291 in the rat γ-aminobutyric acid transporter rGAT-1 in CELLULAR AND MOLECULAR LIFE SCIENCES
  • 2007-04. Direct evidence that two cysteines in the dopamine transporter form a disulfide bond in MOLECULAR AND CELLULAR BIOCHEMISTRY
  • 2007-08. Site-directed alkylation of cysteine to test solvent accessibility of membrane proteins in NATURE PROTOCOLS
  • 2006-09. Serotonin Transporters – Structure and Function in THE JOURNAL OF MEMBRANE BIOLOGY
  • 1991-03. Expression cloning of a cocaine-and antidepressant-sensitive human noradrenaline transporter in NATURE
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00232-012-9492-9

    DOI

    http://dx.doi.org/10.1007/s00232-012-9492-9

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1047171906

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/22918627


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