Mechanical Force and Cytoplasmic Ca2+ Activate Yeast TRPY1 in Parallel View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2009-02

AUTHORS

Zhenwei Su, Xinliang Zhou, Stephen H. Loukin, Yoshiro Saimi, Ching Kung

ABSTRACT

The ability to sense mechanical and osmotic stimuli is vital to all organisms from mammals to bacteria. Members of the transient receptor potential (TRP) ion-channel family have attracted intense attention for their involvement in mechanosensation. The yeast homologue TRPY1 can clearly be activated by hypertonic shock in vivo and by stretch force under patch clamp. Like its animal counterparts, TRPY1 is polymodal, being gated by membrane stretch force and by cytoplasmic Ca(2+). Here, we investigated how these two gating principles interact. We found that stretch force can induce some channel activation without cytoplasmic Ca(2+). Tens of micromolar Ca(2+) greatly enhance the observed force-induced activities, with open probabilities following well the Boltzmann distribution, in which the two gating energies are summed as exponents. To map this formalism to structures, we found Ca(2+)-binding proteins such as calmodulin or calcineurin to be unnecessary. However, removing a dense cluster of negative charges in the C-terminal cytoplasmic domain of TRPY1 greatly diminishes the Ca(2+) activation as well as its influence on force activation. We also found a strategic point upstream of this charge cluster, at which insertion of amino acids weakens Ca(2+) activation considerably but leaves the mechanosensitivity nearly intact. These results led to a structure-function model in which Ca(2+) binding to the cytoplasmic domain and stretching of the membrane-embedded domain both generate gating force, reaching the gate in parallel. More... »

PAGES

141

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00232-009-9153-9

DOI

http://dx.doi.org/10.1007/s00232-009-9153-9

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1011152241

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/19219385


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Biomechanical Phenomena", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Calcineurin", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Calcium", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Calmodulin", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cytoplasm", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Electrophysiology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutagenesis, Site-Directed", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Patch-Clamp Techniques", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Saccharomyces cerevisiae Proteins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transient Receptor Potential Channels", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Wisconsin\u2013Madison", 
          "id": "https://www.grid.ac/institutes/grid.14003.36", 
          "name": [
            "Laboratory of Molecular Biology, University of Wisconsin\u2013Madison, 305 R. M. Bock Laboratories, 1525 Linden Drive, 53706, Madison, WI, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Su", 
        "givenName": "Zhenwei", 
        "id": "sg:person.014756600347.32", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014756600347.32"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Wisconsin\u2013Madison", 
          "id": "https://www.grid.ac/institutes/grid.14003.36", 
          "name": [
            "Laboratory of Molecular Biology, University of Wisconsin\u2013Madison, 305 R. M. Bock Laboratories, 1525 Linden Drive, 53706, Madison, WI, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Zhou", 
        "givenName": "Xinliang", 
        "id": "sg:person.01341063771.97", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01341063771.97"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Wisconsin\u2013Madison", 
          "id": "https://www.grid.ac/institutes/grid.14003.36", 
          "name": [
            "Laboratory of Molecular Biology, University of Wisconsin\u2013Madison, 305 R. M. Bock Laboratories, 1525 Linden Drive, 53706, Madison, WI, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Loukin", 
        "givenName": "Stephen H.", 
        "id": "sg:person.010440252567.79", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010440252567.79"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Wisconsin\u2013Madison", 
          "id": "https://www.grid.ac/institutes/grid.14003.36", 
          "name": [
            "Laboratory of Molecular Biology, University of Wisconsin\u2013Madison, 305 R. M. Bock Laboratories, 1525 Linden Drive, 53706, Madison, WI, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Saimi", 
        "givenName": "Yoshiro", 
        "id": "sg:person.0606070372.01", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0606070372.01"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Wisconsin\u2013Madison", 
          "id": "https://www.grid.ac/institutes/grid.14003.36", 
          "name": [
            "Laboratory of Molecular Biology, University of Wisconsin\u2013Madison, 305 R. M. Bock Laboratories, 1525 Linden Drive, 53706, Madison, WI, USA", 
            "Department of Genetics, University of Wisconsin\u2013Madison, 53706, Madison, WI, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Kung", 
        "givenName": "Ching", 
        "id": "sg:person.01360027630.66", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01360027630.66"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1074/jbc.m803568200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000709385"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m607849200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002873906"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.141036198", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004129775"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1152/physrev.00005.2008", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005159133"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0708584104", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005869529"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.271.38.23357", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007347238"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1083/jcb.200111004", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008266374"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m411089200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1009525895"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0092-8674(02)00719-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010221844"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.4067/s0716-97602006000300003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013801155"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1085/jgp.20028721", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014497440"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nn1843", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015824551", 
          "https://doi.org/10.1038/nn1843"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0960-9822(03)00431-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018517565"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0960-9822(03)00431-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018517565"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0092-8674(91)90547-c", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018521885"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrn2149", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018899702", 
          "https://doi.org/10.1038/nrn2149"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.physiol.64.100301.111649", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020835305"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1085/jgp.20028605", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021385339"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ceca.2005.06.028", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021561626"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.ceca.2005.06.028", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021561626"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nn1665", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021696391", 
          "https://doi.org/10.1038/nn1665"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(89)90358-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021797759"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(89)90358-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021797759"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.biocel.2007.03.013", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026327326"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0711835105", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030044977"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nn.2169", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031246864", 
          "https://doi.org/10.1038/nn.2169"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.physiol.68.040204.100431", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032055653"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.physiol.68.040204.100431", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032055653"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1096/fj.07-101410", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032898175"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1074/jbc.m210810200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1032933590"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.biochem.75.103004.142819", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033906151"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature02732", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034791748", 
          "https://doi.org/10.1038/nature02732"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature02732", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034791748", 
          "https://doi.org/10.1038/nature02732"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1085/jgp.110.3.257", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036251821"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1152/ajpcell.00365.2003", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036948462"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature03896", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040837309", 
          "https://doi.org/10.1038/nature03896"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature03896", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040837309", 
          "https://doi.org/10.1038/nature03896"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nature03896", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040837309", 
          "https://doi.org/10.1038/nature03896"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.1230540100", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043219465"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nrn2117", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044482013", 
          "https://doi.org/10.1038/nrn2117"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/417515a", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044978667", 
          "https://doi.org/10.1038/417515a"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.0704039104", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048500497"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.87.20.7824", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049609324"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/j.neuron.2004.05.001", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053145641"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078848045", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "2009-02", 
    "datePublishedReg": "2009-02-01", 
    "description": "The ability to sense mechanical and osmotic stimuli is vital to all organisms from mammals to bacteria. Members of the transient receptor potential (TRP) ion-channel family have attracted intense attention for their involvement in mechanosensation. The yeast homologue TRPY1 can clearly be activated by hypertonic shock in vivo and by stretch force under patch clamp. Like its animal counterparts, TRPY1 is polymodal, being gated by membrane stretch force and by cytoplasmic Ca(2+). Here, we investigated how these two gating principles interact. We found that stretch force can induce some channel activation without cytoplasmic Ca(2+). Tens of micromolar Ca(2+) greatly enhance the observed force-induced activities, with open probabilities following well the Boltzmann distribution, in which the two gating energies are summed as exponents. To map this formalism to structures, we found Ca(2+)-binding proteins such as calmodulin or calcineurin to be unnecessary. However, removing a dense cluster of negative charges in the C-terminal cytoplasmic domain of TRPY1 greatly diminishes the Ca(2+) activation as well as its influence on force activation. We also found a strategic point upstream of this charge cluster, at which insertion of amino acids weakens Ca(2+) activation considerably but leaves the mechanosensitivity nearly intact. These results led to a structure-function model in which Ca(2+) binding to the cytoplasmic domain and stretching of the membrane-embedded domain both generate gating force, reaching the gate in parallel.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/s00232-009-9153-9", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isFundedItemOf": [
      {
        "id": "sg:grant.2515135", 
        "type": "MonetaryGrant"
      }, 
      {
        "id": "sg:grant.2513824", 
        "type": "MonetaryGrant"
      }
    ], 
    "isPartOf": [
      {
        "id": "sg:journal.1012006", 
        "issn": [
          "0022-2631", 
          "1432-1424"
        ], 
        "name": "The Journal of Membrane Biology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "3", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "227"
      }
    ], 
    "name": "Mechanical Force and Cytoplasmic Ca2+ Activate Yeast TRPY1 in Parallel", 
    "pagination": "141", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "35221a1ee5f03f0e52384ce57dcfc008e1911799cea3eb8605b19dc10bf18569"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "19219385"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0211301"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/s00232-009-9153-9"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1011152241"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/s00232-009-9153-9", 
      "https://app.dimensions.ai/details/publication/pub.1011152241"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T14:26", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000373_0000000373/records_13071_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007%2Fs00232-009-9153-9"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00232-009-9153-9'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00232-009-9153-9'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00232-009-9153-9'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00232-009-9153-9'


 

This table displays all metadata directly associated to this object as RDF triples.

263 TRIPLES      21 PREDICATES      77 URIs      31 LITERALS      19 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/s00232-009-9153-9 schema:about N6140e67249304cff9bd32c2ffd690cfa
2 N6a0fac89ea1245728c8f7cd887ce227a
3 N732ca1079e8745c49b191bd54c214ce2
4 N80afe28d07934fa38b419dd0a6d0ae5b
5 N851f17fe6dde47bdaf701e1ea3ff9478
6 N856b38e038e34d92b1bd15a9cdc20eae
7 N85ec3756786c42b9a047ac43feb56511
8 Ndadbaad828014c9085e5abb4be6ed031
9 Ne4eeccb35df24780a9dad3cd2f7edd54
10 Nf7c1f63ef498478c907bef021de0cf74
11 anzsrc-for:06
12 anzsrc-for:0601
13 schema:author N491f95c05c524489a73c7cd5640db34c
14 schema:citation sg:pub.10.1038/417515a
15 sg:pub.10.1038/nature02732
16 sg:pub.10.1038/nature03896
17 sg:pub.10.1038/nn.2169
18 sg:pub.10.1038/nn1665
19 sg:pub.10.1038/nn1843
20 sg:pub.10.1038/nrn2117
21 sg:pub.10.1038/nrn2149
22 https://app.dimensions.ai/details/publication/pub.1078848045
23 https://doi.org/10.1016/0092-8674(91)90547-c
24 https://doi.org/10.1016/0378-1119(89)90358-2
25 https://doi.org/10.1016/j.biocel.2007.03.013
26 https://doi.org/10.1016/j.ceca.2005.06.028
27 https://doi.org/10.1016/j.neuron.2004.05.001
28 https://doi.org/10.1016/s0092-8674(02)00719-5
29 https://doi.org/10.1016/s0960-9822(03)00431-7
30 https://doi.org/10.1073/pnas.0704039104
31 https://doi.org/10.1073/pnas.0708584104
32 https://doi.org/10.1073/pnas.0711835105
33 https://doi.org/10.1073/pnas.1230540100
34 https://doi.org/10.1073/pnas.141036198
35 https://doi.org/10.1073/pnas.87.20.7824
36 https://doi.org/10.1074/jbc.271.38.23357
37 https://doi.org/10.1074/jbc.m210810200
38 https://doi.org/10.1074/jbc.m411089200
39 https://doi.org/10.1074/jbc.m607849200
40 https://doi.org/10.1074/jbc.m803568200
41 https://doi.org/10.1083/jcb.200111004
42 https://doi.org/10.1085/jgp.110.3.257
43 https://doi.org/10.1085/jgp.20028605
44 https://doi.org/10.1085/jgp.20028721
45 https://doi.org/10.1096/fj.07-101410
46 https://doi.org/10.1146/annurev.biochem.75.103004.142819
47 https://doi.org/10.1146/annurev.physiol.64.100301.111649
48 https://doi.org/10.1146/annurev.physiol.68.040204.100431
49 https://doi.org/10.1152/ajpcell.00365.2003
50 https://doi.org/10.1152/physrev.00005.2008
51 https://doi.org/10.4067/s0716-97602006000300003
52 schema:datePublished 2009-02
53 schema:datePublishedReg 2009-02-01
54 schema:description The ability to sense mechanical and osmotic stimuli is vital to all organisms from mammals to bacteria. Members of the transient receptor potential (TRP) ion-channel family have attracted intense attention for their involvement in mechanosensation. The yeast homologue TRPY1 can clearly be activated by hypertonic shock in vivo and by stretch force under patch clamp. Like its animal counterparts, TRPY1 is polymodal, being gated by membrane stretch force and by cytoplasmic Ca(2+). Here, we investigated how these two gating principles interact. We found that stretch force can induce some channel activation without cytoplasmic Ca(2+). Tens of micromolar Ca(2+) greatly enhance the observed force-induced activities, with open probabilities following well the Boltzmann distribution, in which the two gating energies are summed as exponents. To map this formalism to structures, we found Ca(2+)-binding proteins such as calmodulin or calcineurin to be unnecessary. However, removing a dense cluster of negative charges in the C-terminal cytoplasmic domain of TRPY1 greatly diminishes the Ca(2+) activation as well as its influence on force activation. We also found a strategic point upstream of this charge cluster, at which insertion of amino acids weakens Ca(2+) activation considerably but leaves the mechanosensitivity nearly intact. These results led to a structure-function model in which Ca(2+) binding to the cytoplasmic domain and stretching of the membrane-embedded domain both generate gating force, reaching the gate in parallel.
55 schema:genre research_article
56 schema:inLanguage en
57 schema:isAccessibleForFree false
58 schema:isPartOf N6807adf50bfc4651a78a7b88910f217c
59 Nd60662e975b54f86869751191f0374ce
60 sg:journal.1012006
61 schema:name Mechanical Force and Cytoplasmic Ca2+ Activate Yeast TRPY1 in Parallel
62 schema:pagination 141
63 schema:productId N1ef8c6c02ab74d689523a17cb9773e73
64 N665361711a3e4b55ab8cd1f7d8b39a86
65 N88f2fc2c424e49c38ef0c25ed93e0dbf
66 N97ea3638972a44cfbca3c084a9cb2b1a
67 Ncd4ce3cb11044dbe874ebf468ad287d4
68 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011152241
69 https://doi.org/10.1007/s00232-009-9153-9
70 schema:sdDatePublished 2019-04-11T14:26
71 schema:sdLicense https://scigraph.springernature.com/explorer/license/
72 schema:sdPublisher N4dad3c56778247a99556f6ae8d607851
73 schema:url http://link.springer.com/10.1007%2Fs00232-009-9153-9
74 sgo:license sg:explorer/license/
75 sgo:sdDataset articles
76 rdf:type schema:ScholarlyArticle
77 N15062e38fdd9488380309db0e7c61eeb rdf:first sg:person.010440252567.79
78 rdf:rest Nec6d6f93816447f48ab4f7acb3fac509
79 N1afd82ed192c477aac49949570c155dd rdf:first sg:person.01341063771.97
80 rdf:rest N15062e38fdd9488380309db0e7c61eeb
81 N1ef8c6c02ab74d689523a17cb9773e73 schema:name pubmed_id
82 schema:value 19219385
83 rdf:type schema:PropertyValue
84 N491f95c05c524489a73c7cd5640db34c rdf:first sg:person.014756600347.32
85 rdf:rest N1afd82ed192c477aac49949570c155dd
86 N4dad3c56778247a99556f6ae8d607851 schema:name Springer Nature - SN SciGraph project
87 rdf:type schema:Organization
88 N6140e67249304cff9bd32c2ffd690cfa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
89 schema:name Saccharomyces cerevisiae Proteins
90 rdf:type schema:DefinedTerm
91 N665361711a3e4b55ab8cd1f7d8b39a86 schema:name dimensions_id
92 schema:value pub.1011152241
93 rdf:type schema:PropertyValue
94 N6807adf50bfc4651a78a7b88910f217c schema:issueNumber 3
95 rdf:type schema:PublicationIssue
96 N6a0fac89ea1245728c8f7cd887ce227a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
97 schema:name Calcineurin
98 rdf:type schema:DefinedTerm
99 N732ca1079e8745c49b191bd54c214ce2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
100 schema:name Mutagenesis, Site-Directed
101 rdf:type schema:DefinedTerm
102 N80afe28d07934fa38b419dd0a6d0ae5b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
103 schema:name Biomechanical Phenomena
104 rdf:type schema:DefinedTerm
105 N851f17fe6dde47bdaf701e1ea3ff9478 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
106 schema:name Electrophysiology
107 rdf:type schema:DefinedTerm
108 N856b38e038e34d92b1bd15a9cdc20eae schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
109 schema:name Calcium
110 rdf:type schema:DefinedTerm
111 N85ec3756786c42b9a047ac43feb56511 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name Patch-Clamp Techniques
113 rdf:type schema:DefinedTerm
114 N88f2fc2c424e49c38ef0c25ed93e0dbf schema:name nlm_unique_id
115 schema:value 0211301
116 rdf:type schema:PropertyValue
117 N97ea3638972a44cfbca3c084a9cb2b1a schema:name readcube_id
118 schema:value 35221a1ee5f03f0e52384ce57dcfc008e1911799cea3eb8605b19dc10bf18569
119 rdf:type schema:PropertyValue
120 Ncd4ce3cb11044dbe874ebf468ad287d4 schema:name doi
121 schema:value 10.1007/s00232-009-9153-9
122 rdf:type schema:PropertyValue
123 Nd60662e975b54f86869751191f0374ce schema:volumeNumber 227
124 rdf:type schema:PublicationVolume
125 Ndadbaad828014c9085e5abb4be6ed031 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
126 schema:name Cytoplasm
127 rdf:type schema:DefinedTerm
128 Ndbc645883c314626b5905be233f69b8d rdf:first sg:person.01360027630.66
129 rdf:rest rdf:nil
130 Ne4eeccb35df24780a9dad3cd2f7edd54 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
131 schema:name Transient Receptor Potential Channels
132 rdf:type schema:DefinedTerm
133 Nec6d6f93816447f48ab4f7acb3fac509 rdf:first sg:person.0606070372.01
134 rdf:rest Ndbc645883c314626b5905be233f69b8d
135 Nf7c1f63ef498478c907bef021de0cf74 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
136 schema:name Calmodulin
137 rdf:type schema:DefinedTerm
138 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
139 schema:name Biological Sciences
140 rdf:type schema:DefinedTerm
141 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
142 schema:name Biochemistry and Cell Biology
143 rdf:type schema:DefinedTerm
144 sg:grant.2513824 http://pending.schema.org/fundedItem sg:pub.10.1007/s00232-009-9153-9
145 rdf:type schema:MonetaryGrant
146 sg:grant.2515135 http://pending.schema.org/fundedItem sg:pub.10.1007/s00232-009-9153-9
147 rdf:type schema:MonetaryGrant
148 sg:journal.1012006 schema:issn 0022-2631
149 1432-1424
150 schema:name The Journal of Membrane Biology
151 rdf:type schema:Periodical
152 sg:person.010440252567.79 schema:affiliation https://www.grid.ac/institutes/grid.14003.36
153 schema:familyName Loukin
154 schema:givenName Stephen H.
155 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010440252567.79
156 rdf:type schema:Person
157 sg:person.01341063771.97 schema:affiliation https://www.grid.ac/institutes/grid.14003.36
158 schema:familyName Zhou
159 schema:givenName Xinliang
160 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01341063771.97
161 rdf:type schema:Person
162 sg:person.01360027630.66 schema:affiliation https://www.grid.ac/institutes/grid.14003.36
163 schema:familyName Kung
164 schema:givenName Ching
165 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01360027630.66
166 rdf:type schema:Person
167 sg:person.014756600347.32 schema:affiliation https://www.grid.ac/institutes/grid.14003.36
168 schema:familyName Su
169 schema:givenName Zhenwei
170 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014756600347.32
171 rdf:type schema:Person
172 sg:person.0606070372.01 schema:affiliation https://www.grid.ac/institutes/grid.14003.36
173 schema:familyName Saimi
174 schema:givenName Yoshiro
175 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0606070372.01
176 rdf:type schema:Person
177 sg:pub.10.1038/417515a schema:sameAs https://app.dimensions.ai/details/publication/pub.1044978667
178 https://doi.org/10.1038/417515a
179 rdf:type schema:CreativeWork
180 sg:pub.10.1038/nature02732 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034791748
181 https://doi.org/10.1038/nature02732
182 rdf:type schema:CreativeWork
183 sg:pub.10.1038/nature03896 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040837309
184 https://doi.org/10.1038/nature03896
185 rdf:type schema:CreativeWork
186 sg:pub.10.1038/nn.2169 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031246864
187 https://doi.org/10.1038/nn.2169
188 rdf:type schema:CreativeWork
189 sg:pub.10.1038/nn1665 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021696391
190 https://doi.org/10.1038/nn1665
191 rdf:type schema:CreativeWork
192 sg:pub.10.1038/nn1843 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015824551
193 https://doi.org/10.1038/nn1843
194 rdf:type schema:CreativeWork
195 sg:pub.10.1038/nrn2117 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044482013
196 https://doi.org/10.1038/nrn2117
197 rdf:type schema:CreativeWork
198 sg:pub.10.1038/nrn2149 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018899702
199 https://doi.org/10.1038/nrn2149
200 rdf:type schema:CreativeWork
201 https://app.dimensions.ai/details/publication/pub.1078848045 schema:CreativeWork
202 https://doi.org/10.1016/0092-8674(91)90547-c schema:sameAs https://app.dimensions.ai/details/publication/pub.1018521885
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1016/0378-1119(89)90358-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021797759
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1016/j.biocel.2007.03.013 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026327326
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1016/j.ceca.2005.06.028 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021561626
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1016/j.neuron.2004.05.001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053145641
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1016/s0092-8674(02)00719-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010221844
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1016/s0960-9822(03)00431-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018517565
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1073/pnas.0704039104 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048500497
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1073/pnas.0708584104 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005869529
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1073/pnas.0711835105 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030044977
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1073/pnas.1230540100 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043219465
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1073/pnas.141036198 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004129775
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1073/pnas.87.20.7824 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049609324
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1074/jbc.271.38.23357 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007347238
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1074/jbc.m210810200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032933590
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1074/jbc.m411089200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009525895
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1074/jbc.m607849200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002873906
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1074/jbc.m803568200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000709385
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1083/jcb.200111004 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008266374
239 rdf:type schema:CreativeWork
240 https://doi.org/10.1085/jgp.110.3.257 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036251821
241 rdf:type schema:CreativeWork
242 https://doi.org/10.1085/jgp.20028605 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021385339
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1085/jgp.20028721 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014497440
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1096/fj.07-101410 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032898175
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1146/annurev.biochem.75.103004.142819 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033906151
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1146/annurev.physiol.64.100301.111649 schema:sameAs https://app.dimensions.ai/details/publication/pub.1020835305
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1146/annurev.physiol.68.040204.100431 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032055653
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1152/ajpcell.00365.2003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036948462
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1152/physrev.00005.2008 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005159133
257 rdf:type schema:CreativeWork
258 https://doi.org/10.4067/s0716-97602006000300003 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013801155
259 rdf:type schema:CreativeWork
260 https://www.grid.ac/institutes/grid.14003.36 schema:alternateName University of Wisconsin–Madison
261 schema:name Department of Genetics, University of Wisconsin–Madison, 53706, Madison, WI, USA
262 Laboratory of Molecular Biology, University of Wisconsin–Madison, 305 R. M. Bock Laboratories, 1525 Linden Drive, 53706, Madison, WI, USA
263 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...