Identification of Full-Length Dentin Matrix Protein 1 in Dentin and Bone View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

2008-05

AUTHORS

Bingzhen Huang, Izabela Maciejewska, Yao Sun, Tao Peng, Disheng Qin, Yongbo Lu, Lynda Bonewald, William T. Butler, Jian Feng, Chunlin Qin

ABSTRACT

Dentin matrix protein 1 (DMP1) has been identified in the extracellular matrix (ECM) of dentin and bone as the processed NH(2)-terminal and COOH-terminal fragment. However, the full-length form of DMP1 has not been identified in these tissues. The focus of this investigation was to search for the intact full-length DMP1 in dentin and bone. We used two types of anti-DMP1 antibodies to identify DMP1: one type specifically recognizes the NH(2)-terminal region and the other type is only reactive to the COOH-terminal region of the DMP1 amino acid sequence. An approximately 105-kDa protein, extracted from the ECM of rat dentin and bone, was recognized by both types of antibodies; and the migration rate of this protein was identical to the recombinant mouse full-length DMP1 made in eukaryotic cells. We concluded that this approximately 105-kDa protein is the full-length form of DMP1, which is considerably less abundant than its processed fragments in the ECM of dentin and bone. We also detected the full-length form of DMP1 and its processed fragments in the extract of dental pulp/odontoblast complex dissected from rat teeth. In addition, immunofluorescence analysis showed that in MC3T3-E1 cells the NH(2)-terminal and COOH-terminal fragments of DMP1 are distributed differently. Our findings indicate that the majority of DMP1 must be cleaved within the cells that synthesize it and that minor amounts of uncleaved DMP1 molecules are secreted into the ECM of dentin and bone. More... »

PAGES

401

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00223-008-9140-7

DOI

http://dx.doi.org/10.1007/s00223-008-9140-7

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1011795529

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/18488132


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