Determination of citric and isocitric acids in fruit juices by capillary isotachophoresis View Full Text


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Article Info

DATE

2001-08

AUTHORS

Jana Sádecká, Jozef Polonský, Peter Šimko, Gabriela Karasová

ABSTRACT

. An isotachophoretic method was developed to determine directly citric and isocitric acids in juice samples. Isotachophoretic separations were performed using a Villa Labeco ZKI 02 column-coupling isotachophoretic analyzer equipped with a conductivity detector. The analytical capillary (160 mm×0.3 mm I.D.) was connected with a preseparation capillary (80 mm×0.8 mm I.D.). The leading electrolyte contained hydrochloric acid (10 mmol/l), β-alanine (pH 3.0), and hydroxyethylcellulose (0.1%). The terminating electrolyte was 5 mmol/l acetic acid. Limit of quantitation for citric acid in the preseparation capillary was 20 mg/l and for isocitric acid in the analytical capillary was 1.2 mg/l. More... »

PAGES

161-164

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s002170100338

DOI

http://dx.doi.org/10.1007/s002170100338

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1052159968


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