Overproduction, purification, and property analysis of an extracellular recombinant fructosyltransferase View Full Text


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Article Info

DATE

2016-07

AUTHORS

Wenwen Guo, Haiquan Yang, Shumin Qiang, You Fan, Wei Shen, Xianzhong Chen

ABSTRACT

Fructooligosaccharides (FOS) have widely used for the manufacture of low-calorie and functional foods, because they can inhibit intestinal pathogenic microorganism growth and increase Ca2+ and Mg2+ absorption. In this study, the fructosyltransferase (FruSG) from Aspergillus niger strain SG610, which is used in the industrial production of FOS, was successfully overexpressed in Pichia pastoris strain GS115 and characterized. Recombinant FruSG had optimum temperature and pH of 50 °C and 5.5, respectively, and it was stable below 40 °C and at from pH 3.0 to 8.0. Km and kcat values of the recombinant FruSG were 424.5 mmol L−1 and 3.8 × 103 min−1, respectively. On addition of 50 and 100 g L−1 glucose, the Ki was 130.6 and 170.0 mmol L−1, respectively. The recombinant FruSG had the highest activity (2294.7 U mL−1) after induction for 144 h in a 5-L fermenter, which was 956.1-fold higher than that of native FruSG from A. niger SG610, and produced a high yield of FOS (64.5 %, w/w). The recombinant FruSG was activated by 5 mmol L−1 Mg2+ and 1 mmol L−1 Fe2+ (relative activity of 125.2 and 120.9 %, respectively), indicating that the enzyme was Mg2+ and Fe2+ dependent. Moreover, toluene, n-butanol, trichloromethane, and ethyl acetate significantly activated FruSG. Together, these results indicate that the recombinant FruSG is an excellent candidate for the industrial production of FOS. More... »

PAGES

1159-1168

References to SciGraph publications

  • 2002-01. Use of certain alcohol ethoxylates to maintain protease stability in the presence of anionic surfactants in JOURNAL OF SURFACTANTS AND DETERGENTS
  • 2000-09. Recombinant protein expression in Pichia pastoris in MOLECULAR BIOTECHNOLOGY
  • 2014-02. Enzymatic Trends of Fructooligosaccharides Production by Microorganisms in APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
  • 1991-05. Production of fructooligosaccharides from sucrose by a transfructosylase from Aspergillus niger in WORLD JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
  • 2008-06. Purification and characterisation of a fructosyltransferase from Rhodotorula sp. in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2010-08. Optimized bioprocess for production of fructofuranosidase by recombinant Aspergillus niger in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 2014-05. Purification and evaluation of the enzymatic properties of a novel fructosyltransferase from Aspergillus oryzae: a potential biocatalyst for the synthesis of sucrose 6-acetate in BIOTECHNOLOGY LETTERS
  • 2009-01. Production of fructosyltransferase by Aureobasidium sp. ATCC 20524 in batch and two-step batch cultures in JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY
  • 2011-02. Gene cloning and enzyme structure modeling of the Aspergillus oryzae N74 fructosyltransferase in MOLECULAR BIOLOGY REPORTS
  • 2013-02. High-level expression, purification, and enzymatic characterization of truncated poly(vinyl alcohol) dehydrogenase in methylotrophic yeast Pichia pastoris in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • Journal

    TITLE

    European Food Research and Technology

    ISSUE

    7

    VOLUME

    242

    Author Affiliations

    Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00217-015-2620-x

    DOI

    http://dx.doi.org/10.1007/s00217-015-2620-x

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1034370080


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