Development of a method for the quantification of haddock (Melanogrammus aeglefinus) in commercial products using real-time PCR View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2004-12-02

AUTHORS

H. J. Hird, G. L. Hold, J. Chisholm, P. Reece, V. J. Russell, J. Brown, R. Goodier, R. MacArthur

ABSTRACT

A method is presented for the quantification of haddock in complex food matrices based on real-time PCR. The proportion of haddock muscle tissue to the numbers of a single copy gene has been shown to remain relatively constant, throughout the year and across a number of fishing grounds, using conventional muscle nitrogen content as a comparator. This indicates that quantification of fish, based upon nitrogen content or the numbers of a single copy gene, would return data with a similar degree of accuracy. Thus, a haddock-specific, real-time PCR primer and probe set was designed and optimised, and when used in conjunction with a haddock calibration curve was shown to be able to quantify model samples to within 7% of the true percentage. This is the first report of a real-time assay for the quantification of white fish, which would allow enforcement of the legislation in a complex food matrix containing haddock with other fish species. More... »

PAGES

633-637

References to SciGraph publications

  • 2001-02. Validation of a PCR-RFLP based method for the identification of salmon species in food products in EUROPEAN FOOD RESEARCH AND TECHNOLOGY
  • 2003-07-15. Identification of gadoid fish species using DNA-based techniques in EUROPEAN FOOD RESEARCH AND TECHNOLOGY
  • 2003-05-22. Detection of peanut using real-time polymerase chain reaction in EUROPEAN FOOD RESEARCH AND TECHNOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/s00217-004-1050-y

    DOI

    http://dx.doi.org/10.1007/s00217-004-1050-y

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1044927627


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Central Science Laboratory, Sand Hutton, YO41 1LZ, York, UK", 
              "id": "http://www.grid.ac/institutes/grid.470556.5", 
              "name": [
                "Central Science Laboratory, Sand Hutton, YO41 1LZ, York, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Hird", 
            "givenName": "H. J.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Department of Medicine and Therapeutics, Aberdeen University, AB25 2ZD, Aberdeen, UK", 
              "id": "http://www.grid.ac/institutes/grid.7107.1", 
              "name": [
                "Department of Medicine and Therapeutics, Aberdeen University, AB25 2ZD, Aberdeen, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Hold", 
            "givenName": "G. L.", 
            "id": "sg:person.01015442527.89", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01015442527.89"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Central Science Laboratory, Sand Hutton, YO41 1LZ, York, UK", 
              "id": "http://www.grid.ac/institutes/grid.470556.5", 
              "name": [
                "Central Science Laboratory, Sand Hutton, YO41 1LZ, York, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Chisholm", 
            "givenName": "J.", 
            "id": "sg:person.0671643765.91", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0671643765.91"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Central Science Laboratory, Sand Hutton, YO41 1LZ, York, UK", 
              "id": "http://www.grid.ac/institutes/grid.470556.5", 
              "name": [
                "Central Science Laboratory, Sand Hutton, YO41 1LZ, York, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Reece", 
            "givenName": "P.", 
            "id": "sg:person.01073152370.70", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01073152370.70"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Rowett Research Institute, Greenburn Road, Bucksburn, AB21 9SB, Aberdeen, UK", 
              "id": "http://www.grid.ac/institutes/grid.7107.1", 
              "name": [
                "Rowett Research Institute, Greenburn Road, Bucksburn, AB21 9SB, Aberdeen, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Russell", 
            "givenName": "V. J.", 
            "id": "sg:person.01020112137.42", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01020112137.42"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Central Science Laboratory, Sand Hutton, YO41 1LZ, York, UK", 
              "id": "http://www.grid.ac/institutes/grid.470556.5", 
              "name": [
                "Central Science Laboratory, Sand Hutton, YO41 1LZ, York, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Brown", 
            "givenName": "J.", 
            "id": "sg:person.01153566173.61", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01153566173.61"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Central Science Laboratory, Sand Hutton, YO41 1LZ, York, UK", 
              "id": "http://www.grid.ac/institutes/grid.470556.5", 
              "name": [
                "Central Science Laboratory, Sand Hutton, YO41 1LZ, York, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Goodier", 
            "givenName": "R.", 
            "id": "sg:person.0664355546.05", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0664355546.05"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Central Science Laboratory, Sand Hutton, YO41 1LZ, York, UK", 
              "id": "http://www.grid.ac/institutes/grid.470556.5", 
              "name": [
                "Central Science Laboratory, Sand Hutton, YO41 1LZ, York, UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "MacArthur", 
            "givenName": "R.", 
            "id": "sg:person.01250433155.04", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01250433155.04"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1007/s002170000237", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052518813", 
              "https://doi.org/10.1007/s002170000237"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00217-003-0735-y", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020884966", 
              "https://doi.org/10.1007/s00217-003-0735-y"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/s00217-003-0726-z", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019864314", 
              "https://doi.org/10.1007/s00217-003-0726-z"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "2004-12-02", 
        "datePublishedReg": "2004-12-02", 
        "description": "A method is presented for the quantification of haddock in complex food matrices based on real-time PCR. The proportion of haddock muscle tissue to the numbers of a single copy gene has been shown to remain relatively constant, throughout the year and across a number of fishing grounds, using conventional muscle nitrogen content as a comparator. This indicates that quantification of fish, based upon nitrogen content or the numbers of a single copy gene, would return data with a similar degree of accuracy. Thus, a haddock-specific, real-time PCR primer and probe set was designed and optimised, and when used in conjunction with a haddock calibration curve was shown to be able to quantify model samples to within 7% of the true percentage. This is the first report of a real-time assay for the quantification of white fish, which would allow enforcement of the legislation in a complex food matrix containing haddock with other fish species.", 
        "genre": "article", 
        "id": "sg:pub.10.1007/s00217-004-1050-y", 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1312167", 
            "issn": [
              "0044-3026", 
              "1438-2385"
            ], 
            "name": "European Food Research and Technology", 
            "publisher": "Springer Nature", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "5-6", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "220"
          }
        ], 
        "keywords": [
          "single-copy gene", 
          "copy gene", 
          "real-time PCR", 
          "quantification of fish", 
          "real-time PCR primers", 
          "fish species", 
          "PCR primers", 
          "probe sets", 
          "nitrogen content", 
          "real-time assay", 
          "fishing grounds", 
          "genes", 
          "haddock", 
          "fish", 
          "muscle tissue", 
          "first report", 
          "PCR", 
          "white fish", 
          "complex food matrices", 
          "species", 
          "primers", 
          "assays", 
          "food matrices", 
          "tissue", 
          "quantification", 
          "commercial products", 
          "number", 
          "content", 
          "similar degree", 
          "development", 
          "matrix", 
          "proportion", 
          "products", 
          "true percentage", 
          "percentage", 
          "data", 
          "set", 
          "conjunction", 
          "ground", 
          "years", 
          "report", 
          "samples", 
          "degree", 
          "legislation", 
          "method", 
          "enforcement", 
          "curves", 
          "accuracy", 
          "model samples", 
          "calibration curve", 
          "comparator"
        ], 
        "name": "Development of a method for the quantification of haddock (Melanogrammus aeglefinus) in commercial products using real-time PCR", 
        "pagination": "633-637", 
        "productId": [
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1044927627"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/s00217-004-1050-y"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/s00217-004-1050-y", 
          "https://app.dimensions.ai/details/publication/pub.1044927627"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2022-11-24T20:51", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-springernature-scigraph/baseset/20221124/entities/gbq_results/article/article_385.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "https://doi.org/10.1007/s00217-004-1050-y"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00217-004-1050-y'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00217-004-1050-y'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00217-004-1050-y'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00217-004-1050-y'


     

    This table displays all metadata directly associated to this object as RDF triples.

    173 TRIPLES      21 PREDICATES      78 URIs      67 LITERALS      6 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/s00217-004-1050-y schema:about anzsrc-for:06
    2 anzsrc-for:0604
    3 schema:author N4deceed6c7a54090b57507224380853a
    4 schema:citation sg:pub.10.1007/s00217-003-0726-z
    5 sg:pub.10.1007/s00217-003-0735-y
    6 sg:pub.10.1007/s002170000237
    7 schema:datePublished 2004-12-02
    8 schema:datePublishedReg 2004-12-02
    9 schema:description A method is presented for the quantification of haddock in complex food matrices based on real-time PCR. The proportion of haddock muscle tissue to the numbers of a single copy gene has been shown to remain relatively constant, throughout the year and across a number of fishing grounds, using conventional muscle nitrogen content as a comparator. This indicates that quantification of fish, based upon nitrogen content or the numbers of a single copy gene, would return data with a similar degree of accuracy. Thus, a haddock-specific, real-time PCR primer and probe set was designed and optimised, and when used in conjunction with a haddock calibration curve was shown to be able to quantify model samples to within 7% of the true percentage. This is the first report of a real-time assay for the quantification of white fish, which would allow enforcement of the legislation in a complex food matrix containing haddock with other fish species.
    10 schema:genre article
    11 schema:isAccessibleForFree false
    12 schema:isPartOf N15b99842d8124bc59c119319a20cf561
    13 Ncbd53348fa7e440ea3ef4d83617b9938
    14 sg:journal.1312167
    15 schema:keywords PCR
    16 PCR primers
    17 accuracy
    18 assays
    19 calibration curve
    20 commercial products
    21 comparator
    22 complex food matrices
    23 conjunction
    24 content
    25 copy gene
    26 curves
    27 data
    28 degree
    29 development
    30 enforcement
    31 first report
    32 fish
    33 fish species
    34 fishing grounds
    35 food matrices
    36 genes
    37 ground
    38 haddock
    39 legislation
    40 matrix
    41 method
    42 model samples
    43 muscle tissue
    44 nitrogen content
    45 number
    46 percentage
    47 primers
    48 probe sets
    49 products
    50 proportion
    51 quantification
    52 quantification of fish
    53 real-time PCR
    54 real-time PCR primers
    55 real-time assay
    56 report
    57 samples
    58 set
    59 similar degree
    60 single-copy gene
    61 species
    62 tissue
    63 true percentage
    64 white fish
    65 years
    66 schema:name Development of a method for the quantification of haddock (Melanogrammus aeglefinus) in commercial products using real-time PCR
    67 schema:pagination 633-637
    68 schema:productId Nb32959a78896483f97f900e58b1ceea3
    69 Nc5618d9356bc499d956610ec8f8eaaa6
    70 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044927627
    71 https://doi.org/10.1007/s00217-004-1050-y
    72 schema:sdDatePublished 2022-11-24T20:51
    73 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    74 schema:sdPublisher N73464d1e5b7c487cb0ec4c346ae8614b
    75 schema:url https://doi.org/10.1007/s00217-004-1050-y
    76 sgo:license sg:explorer/license/
    77 sgo:sdDataset articles
    78 rdf:type schema:ScholarlyArticle
    79 N0452bf7ff55943e294f895da5ba943f5 rdf:first sg:person.0671643765.91
    80 rdf:rest Ned0b944449734f6dbb58786666a0b696
    81 N15b99842d8124bc59c119319a20cf561 schema:volumeNumber 220
    82 rdf:type schema:PublicationVolume
    83 N4deceed6c7a54090b57507224380853a rdf:first N808ac3864b0543b5849b68cc3aeab18f
    84 rdf:rest Nf0b59bc39ee24d989535f50454f2d042
    85 N55e34e4f629440a7afac7ec3352e01e7 rdf:first sg:person.0664355546.05
    86 rdf:rest N661f14ead69e4b96a93e068bb042054c
    87 N661f14ead69e4b96a93e068bb042054c rdf:first sg:person.01250433155.04
    88 rdf:rest rdf:nil
    89 N73464d1e5b7c487cb0ec4c346ae8614b schema:name Springer Nature - SN SciGraph project
    90 rdf:type schema:Organization
    91 N808ac3864b0543b5849b68cc3aeab18f schema:affiliation grid-institutes:grid.470556.5
    92 schema:familyName Hird
    93 schema:givenName H. J.
    94 rdf:type schema:Person
    95 N8f206d33b3fa4285b4e9ba2d8085fdbe rdf:first sg:person.01020112137.42
    96 rdf:rest Nfaa384f8da214b5f8803fe726febe8c8
    97 Nb32959a78896483f97f900e58b1ceea3 schema:name dimensions_id
    98 schema:value pub.1044927627
    99 rdf:type schema:PropertyValue
    100 Nc5618d9356bc499d956610ec8f8eaaa6 schema:name doi
    101 schema:value 10.1007/s00217-004-1050-y
    102 rdf:type schema:PropertyValue
    103 Ncbd53348fa7e440ea3ef4d83617b9938 schema:issueNumber 5-6
    104 rdf:type schema:PublicationIssue
    105 Ned0b944449734f6dbb58786666a0b696 rdf:first sg:person.01073152370.70
    106 rdf:rest N8f206d33b3fa4285b4e9ba2d8085fdbe
    107 Nf0b59bc39ee24d989535f50454f2d042 rdf:first sg:person.01015442527.89
    108 rdf:rest N0452bf7ff55943e294f895da5ba943f5
    109 Nfaa384f8da214b5f8803fe726febe8c8 rdf:first sg:person.01153566173.61
    110 rdf:rest N55e34e4f629440a7afac7ec3352e01e7
    111 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    112 schema:name Biological Sciences
    113 rdf:type schema:DefinedTerm
    114 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    115 schema:name Genetics
    116 rdf:type schema:DefinedTerm
    117 sg:journal.1312167 schema:issn 0044-3026
    118 1438-2385
    119 schema:name European Food Research and Technology
    120 schema:publisher Springer Nature
    121 rdf:type schema:Periodical
    122 sg:person.01015442527.89 schema:affiliation grid-institutes:grid.7107.1
    123 schema:familyName Hold
    124 schema:givenName G. L.
    125 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01015442527.89
    126 rdf:type schema:Person
    127 sg:person.01020112137.42 schema:affiliation grid-institutes:grid.7107.1
    128 schema:familyName Russell
    129 schema:givenName V. J.
    130 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01020112137.42
    131 rdf:type schema:Person
    132 sg:person.01073152370.70 schema:affiliation grid-institutes:grid.470556.5
    133 schema:familyName Reece
    134 schema:givenName P.
    135 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01073152370.70
    136 rdf:type schema:Person
    137 sg:person.01153566173.61 schema:affiliation grid-institutes:grid.470556.5
    138 schema:familyName Brown
    139 schema:givenName J.
    140 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01153566173.61
    141 rdf:type schema:Person
    142 sg:person.01250433155.04 schema:affiliation grid-institutes:grid.470556.5
    143 schema:familyName MacArthur
    144 schema:givenName R.
    145 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01250433155.04
    146 rdf:type schema:Person
    147 sg:person.0664355546.05 schema:affiliation grid-institutes:grid.470556.5
    148 schema:familyName Goodier
    149 schema:givenName R.
    150 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0664355546.05
    151 rdf:type schema:Person
    152 sg:person.0671643765.91 schema:affiliation grid-institutes:grid.470556.5
    153 schema:familyName Chisholm
    154 schema:givenName J.
    155 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0671643765.91
    156 rdf:type schema:Person
    157 sg:pub.10.1007/s00217-003-0726-z schema:sameAs https://app.dimensions.ai/details/publication/pub.1019864314
    158 https://doi.org/10.1007/s00217-003-0726-z
    159 rdf:type schema:CreativeWork
    160 sg:pub.10.1007/s00217-003-0735-y schema:sameAs https://app.dimensions.ai/details/publication/pub.1020884966
    161 https://doi.org/10.1007/s00217-003-0735-y
    162 rdf:type schema:CreativeWork
    163 sg:pub.10.1007/s002170000237 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052518813
    164 https://doi.org/10.1007/s002170000237
    165 rdf:type schema:CreativeWork
    166 grid-institutes:grid.470556.5 schema:alternateName Central Science Laboratory, Sand Hutton, YO41 1LZ, York, UK
    167 schema:name Central Science Laboratory, Sand Hutton, YO41 1LZ, York, UK
    168 rdf:type schema:Organization
    169 grid-institutes:grid.7107.1 schema:alternateName Department of Medicine and Therapeutics, Aberdeen University, AB25 2ZD, Aberdeen, UK
    170 Rowett Research Institute, Greenburn Road, Bucksburn, AB21 9SB, Aberdeen, UK
    171 schema:name Department of Medicine and Therapeutics, Aberdeen University, AB25 2ZD, Aberdeen, UK
    172 Rowett Research Institute, Greenburn Road, Bucksburn, AB21 9SB, Aberdeen, UK
    173 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...