Differentiation of raw or processed eel by PCR-based techniques: restriction fragment length polymorphism analysis (RFLP) and single strand conformation polymorphism ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2002-02

AUTHORS

Hartmut Rehbein, Carmen G. Sotelo, Ricardo I. Perez-Martin, M.-Jose Chapela-Garrido, Georgina L. Hold, Valerie J. Russell, Susan E. Pryde, Ana T. Santos, Carla Rosa, Javier Quinteiro, Manuel Rey-Mendez

ABSTRACT

PCR-based methods have been developed to differentiate four species of commercially important eels (Anguilla anguilla, A. rostrata, A. japonica, A. australis). By means of universal primers a 464 base pair (bp) section of the cytochrome b gene was amplified. The amplicon was sequenced and restriction enzymes were selected for RFLP analysis; a short part (123 bp) of the 464 bp section was amplified by another set of primers and used for SSCP analysis.The suitability of RFLP and SSCP for differentiating hot-smoked eel was verified by a collaborative study. More... »

PAGES

171-177

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00217-001-0457-y

DOI

http://dx.doi.org/10.1007/s00217-001-0457-y

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1036465888


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176 schema:name Rowett Research Institute, Greenburn Road, Aberdeen, AB21 9SB, Scotland, UK, UK
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