FRET-based fluorescent probe for drug assay from amino acid@gold-carbon nanoparticles View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

2021-01-06

AUTHORS

Mehavesh K. Hameed, Javad B. M. Parambath, Sofian M. Kanan, Ahmed A. Mohamed

ABSTRACT

Biocompatible and luminescent nanostructures synthesized by capping gold-carbon nanoparticles (HOOC-4-C6H4-AuNPs) with amino acids tyrosine, tryptophan, and cysteine were used for the quantitative estimation of ranitidine (RNH), a peptic ulcer and gastroesophageal reflux drug. We applied a fluorescence quenching mechanism to investigate the viability of the energy transfer based on gold-carbon nanosensors. Förster resonance energy transfer (FRET) calculations showed a donor–acceptor distance of 1.69 nm (Tyr@AuNPs), 2.27 nm (Trp@AuNPs), and 2.32 nm (Cys@AuNPs). The constant time-resolved fluorescence lifetime measurements supported the static quenching nature. This method was successfully utilized in the detection and quantification of RNH, with a limit of detection (LOD) of 0.174, 0.56, and 0.332 μM for Tyr@AuNP, Trp@AuNP, and Cys@AuNP bioconjugates, respectively. This approach was also successful in the quantification of RNH in spiked serum samples. More... »

PAGES

1117-1125

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/s00216-020-03075-9

DOI

http://dx.doi.org/10.1007/s00216-020-03075-9

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1134393999

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/33409672


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