Ontology type: schema:ScholarlyArticle
2017-11-21
AUTHORSBum Jin Kim, Chanyoung Han, Hantae Moon, Joseph Kwon, Ik-Soon Jang, Si-Keun Lim, Ki-Won Park, Jong-Soon Choi, Hyun Joo An
ABSTRACTThe estimation of post-mortem interval (PMI) is a crucial part for investigations of crime and untimely deaths in forensic science. However, standard methods of PMI estimation are easily confounded by extenuating circumstances and/or environmental factors. Therefore, a panel of PMI markers obtained from a more acceptable and accurate method is necessary to definitely determine time of death. Saliva, one of the vital fluids encountered at crime scenes, contains various glycoproteins that are highly affected by biochemical environment. Here, we investigated saliva N-glycans between live and dead rats to determine the alteration of N-glycans using an animal model system because of the limitation of saliva collection from recently deceased humans. Rat saliva samples were collected both before and after death. N-Glycans were enzymatically released by PNGase F without any glycoprotein extraction. Released native glycans were purified and enriched by PGC-SPE. About 100 N-glycans were identified, profiled, and structurally elucidated by nano LC/MS and tandem MS. Sialylated N-glycans were exclusively present in abundance in live rat saliva whereas non-sialylated N-glycans including LacdiNAc disaccharides were detected in high level following death. Through in-depth investigations using quantitative comparison and statistical analysis, 14 N-glycans that significantly changed after death were identified as the potential marker candidates for PMI estimation. To the best of our knowledge, this is the first study to monitor the post-mortem changes of saliva glycosylation, with obvious forensic applications. More... »
PAGES45-56
http://scigraph.springernature.com/pub.10.1007/s00216-017-0702-2
DOIhttp://dx.doi.org/10.1007/s00216-017-0702-2
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1092807916
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/29164281
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/03",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Chemical Sciences",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/09",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Engineering",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Animals",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Autopsy",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Chromatography, Liquid",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Forensic Medicine",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Glycosylation",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Humans",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Polysaccharides",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Postmortem Changes",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Rats",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Rats, Sprague-Dawley",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Saliva",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Tandem Mass Spectrometry",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Asia-Pacific Glycomics Reference Site, Chungnam National University, Daejeon, Korea",
"id": "http://www.grid.ac/institutes/grid.254230.2",
"name": [
"Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, Korea",
"Asia-Pacific Glycomics Reference Site, Chungnam National University, Daejeon, Korea"
],
"type": "Organization"
},
"familyName": "Kim",
"givenName": "Bum Jin",
"id": "sg:person.0632042225.42",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0632042225.42"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Asia-Pacific Glycomics Reference Site, Chungnam National University, Daejeon, Korea",
"id": "http://www.grid.ac/institutes/grid.254230.2",
"name": [
"Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, Korea",
"Asia-Pacific Glycomics Reference Site, Chungnam National University, Daejeon, Korea"
],
"type": "Organization"
},
"familyName": "Han",
"givenName": "Chanyoung",
"id": "sg:person.01211264107.34",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01211264107.34"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Asia-Pacific Glycomics Reference Site, Chungnam National University, Daejeon, Korea",
"id": "http://www.grid.ac/institutes/grid.254230.2",
"name": [
"Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, Korea",
"Asia-Pacific Glycomics Reference Site, Chungnam National University, Daejeon, Korea"
],
"type": "Organization"
},
"familyName": "Moon",
"givenName": "Hantae",
"id": "sg:person.010341734101.19",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010341734101.19"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Biological Disaster Analysis Team, Korea Basic Science Institute, Daejeon, Korea",
"id": "http://www.grid.ac/institutes/grid.410885.0",
"name": [
"Biological Disaster Analysis Team, Korea Basic Science Institute, Daejeon, Korea"
],
"type": "Organization"
},
"familyName": "Kwon",
"givenName": "Joseph",
"id": "sg:person.0662776456.12",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0662776456.12"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Biological Disaster Analysis Team, Korea Basic Science Institute, Daejeon, Korea",
"id": "http://www.grid.ac/institutes/grid.410885.0",
"name": [
"Biological Disaster Analysis Team, Korea Basic Science Institute, Daejeon, Korea"
],
"type": "Organization"
},
"familyName": "Jang",
"givenName": "Ik-Soon",
"id": "sg:person.01317070447.13",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01317070447.13"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Forensic DNA Division, National Forensic Service, Wonju, Korea",
"id": "http://www.grid.ac/institutes/grid.419645.b",
"name": [
"Forensic DNA Division, National Forensic Service, Wonju, Korea"
],
"type": "Organization"
},
"familyName": "Lim",
"givenName": "Si-Keun",
"id": "sg:person.011217423071.29",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.011217423071.29"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Forensic DNA Division, National Forensic Service, Wonju, Korea",
"id": "http://www.grid.ac/institutes/grid.419645.b",
"name": [
"Forensic DNA Division, National Forensic Service, Wonju, Korea"
],
"type": "Organization"
},
"familyName": "Park",
"givenName": "Ki-Won",
"id": "sg:person.01107410511.39",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01107410511.39"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Biological Disaster Analysis Team, Korea Basic Science Institute, Daejeon, Korea",
"id": "http://www.grid.ac/institutes/grid.410885.0",
"name": [
"Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, Korea",
"Biological Disaster Analysis Team, Korea Basic Science Institute, Daejeon, Korea"
],
"type": "Organization"
},
"familyName": "Choi",
"givenName": "Jong-Soon",
"id": "sg:person.01266030275.44",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01266030275.44"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Asia-Pacific Glycomics Reference Site, Chungnam National University, Daejeon, Korea",
"id": "http://www.grid.ac/institutes/grid.254230.2",
"name": [
"Graduate School of Analytical Science and Technology, Chungnam National University, Daejeon, Korea",
"Asia-Pacific Glycomics Reference Site, Chungnam National University, Daejeon, Korea"
],
"type": "Organization"
},
"familyName": "An",
"givenName": "Hyun Joo",
"id": "sg:person.01002675157.10",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01002675157.10"
],
"type": "Person"
}
],
"citation": [
{
"id": "sg:pub.10.1007/978-1-4615-1267-7_21",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1013361537",
"https://doi.org/10.1007/978-1-4615-1267-7_21"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s10719-007-9071-y",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1019974219",
"https://doi.org/10.1007/s10719-007-9071-y"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/srep12844",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1025259370",
"https://doi.org/10.1038/srep12844"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/srep04963",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1015809367",
"https://doi.org/10.1038/srep04963"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s00216-016-9355-9",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1021588203",
"https://doi.org/10.1007/s00216-016-9355-9"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s11306-016-1118-2",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1021468189",
"https://doi.org/10.1007/s11306-016-1118-2"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s12014-008-9021-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1048119459",
"https://doi.org/10.1007/s12014-008-9021-0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/s11306-014-0691-5",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044480201",
"https://doi.org/10.1007/s11306-014-0691-5"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/cdd.2013.50",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1046171448",
"https://doi.org/10.1038/cdd.2013.50"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/srep24197",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1030839136",
"https://doi.org/10.1038/srep24197"
],
"type": "CreativeWork"
}
],
"datePublished": "2017-11-21",
"datePublishedReg": "2017-11-21",
"description": "The estimation of post-mortem interval (PMI) is a crucial part for investigations of crime and untimely deaths in forensic science. However, standard methods of PMI estimation are easily confounded by extenuating circumstances and/or environmental factors. Therefore, a panel of PMI markers obtained from a more acceptable and accurate method is necessary to definitely determine time of death. Saliva, one of the vital fluids encountered at crime scenes, contains various glycoproteins that are highly affected by biochemical environment. Here, we investigated saliva N-glycans between live and dead rats to determine the alteration of N-glycans using an animal model system because of the limitation of saliva collection from recently deceased humans. Rat saliva samples were collected both before and after death. N-Glycans were enzymatically released by PNGase F without any glycoprotein extraction. Released native glycans were purified and enriched by PGC-SPE. About 100 N-glycans were identified, profiled, and structurally elucidated by nano LC/MS and tandem MS. Sialylated N-glycans were exclusively present in abundance in live rat saliva whereas non-sialylated N-glycans including LacdiNAc disaccharides were detected in high level following death. Through in-depth investigations using quantitative comparison and statistical analysis, 14 N-glycans that significantly changed after death were identified as the potential marker candidates for PMI estimation. To the best of our knowledge, this is the first study to monitor the post-mortem changes of saliva glycosylation, with obvious forensic applications.",
"genre": "article",
"id": "sg:pub.10.1007/s00216-017-0702-2",
"inLanguage": "en",
"isAccessibleForFree": false,
"isPartOf": [
{
"id": "sg:journal.1358693",
"issn": [
"1618-2642",
"1618-2650"
],
"name": "Analytical and Bioanalytical Chemistry",
"publisher": "Springer Nature",
"type": "Periodical"
},
{
"issueNumber": "1",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "410"
}
],
"keywords": [
"estimation",
"post-mortem interval",
"intervals",
"crucial part",
"part",
"investigation",
"untimely death",
"death",
"forensic science",
"science",
"standard methods",
"method",
"PMI estimation",
"circumstances",
"environmental factors",
"factors",
"panel",
"markers",
"accurate method",
"time of death",
"time",
"saliva",
"vital fluids",
"fluid",
"glycoprotein",
"biochemical environment",
"glycans",
"dead rats",
"rats",
"alterations",
"animal model systems",
"model system",
"system",
"limitations",
"saliva collection",
"collection",
"deceased humans",
"humans",
"saliva samples",
"samples",
"PNGase F",
"LC/MS",
"MS",
"tandem MS",
"Sialylated N",
"rat saliva",
"disaccharides",
"high levels",
"levels",
"depth investigation",
"quantitative comparison",
"comparison",
"statistical analysis",
"analysis",
"potential marker candidates",
"marker candidates",
"candidates",
"knowledge",
"first study",
"study",
"post-mortem changes",
"changes",
"glycosylation",
"forensic applications",
"applications",
"investigation of crimes",
"crime",
"PMI markers",
"crime scene",
"scene",
"environment",
"saliva N",
"Rat saliva samples",
"glycoprotein extraction",
"extraction",
"native glycans",
"PGC-SPE",
"nano LC/MS",
"abundance",
"live rat saliva",
"LacdiNAc disaccharides",
"saliva glycosylation",
"obvious forensic applications"
],
"name": "Monitoring of post-mortem changes of saliva N-glycosylation by nano LC/MS",
"pagination": "45-56",
"productId": [
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1092807916"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/s00216-017-0702-2"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"29164281"
]
}
],
"sameAs": [
"https://doi.org/10.1007/s00216-017-0702-2",
"https://app.dimensions.ai/details/publication/pub.1092807916"
],
"sdDataset": "articles",
"sdDatePublished": "2022-01-01T18:42",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-springernature-scigraph/baseset/20220101/entities/gbq_results/article/article_739.jsonl",
"type": "ScholarlyArticle",
"url": "https://doi.org/10.1007/s00216-017-0702-2"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/s00216-017-0702-2'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/s00216-017-0702-2'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/s00216-017-0702-2'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/s00216-017-0702-2'
This table displays all metadata directly associated to this object as RDF triples.
305 TRIPLES
22 PREDICATES
133 URIs
114 LITERALS
20 BLANK NODES